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Meiji Techno HD1500MET Instruction Manual

Image acquisition, managing and processing software iscapture
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HD1500MET image acquisition, managing and
processing
software
ISCapture
Instruction Manual
 Key to the Instruction Manual
 ISC is shortened name used for ISCapture.
 Square brackets are used to indicate items
such as menu names, button names and window names
that appear on the computer screen.
 Help
 Refer to [Help] >> [About ISCapture] menu for
software information and technical support.
 Provide the following information when it is
required to obtain the technical support:
Camera model and S/N (serial number);
1
2
Software version number;
3
Description of the problem. Screenshots of the
problem would be useful.
System requirements
OS
Windows XP/ Vista/ 7/ 8 (32 & 64bit)
CPU
Intel processor (Core2 Duo or higher is
recommended)
Memory
2GB or More is recommended
USB ports
USB2.0 Hi-Speed port
1

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Summary of Contents for Meiji Techno HD1500MET

  • Page 1 HD1500MET image acquisition, managing and required to obtain the technical support: processing software Camera model and S/N (serial number); ISCapture Software version number; Instruction Manual Description of the problem. Screenshots of the  Key to the Instruction Manual problem would be useful.

  • Page 2: Table Of Contents

    ......................21 ARAMETER CHAPTER 1: HD1500MET CAMERA QUICK START..........3 ..................21 MAGE EASUREMENT ..................... 3 AMERA NSTALLATION Measurement List..................22 ..................3 RIVER NSTALLATION Edit Scale Line....................23 ................... 4 OFTWARE NSTALLATION Create Calibration File.................. 24 ....................4 TART APTURE Calibration Table................... 26 ................5...

  • Page 3: Chapter 1: Hd1500Met Camera Quick Start

    Chapter 1: Driver Installation HD1500MET Camera Quick Start 1. Connect the camera to the PC. Insert the CD comes with the camera, copy the driver “Tucsen Camera Driver (H Series) Camera Installation Setup.exe” to the computer. Double click on it to start the installation.

  • Page 4: Software Installation

    created on the desktop. Software Installation Note: If previous version ISCapture was installed in your PC, the 1. Copy the software ISCapture installer to the PC. Double-click on installer will automatically detect it and ask to remove it first it to start the installation. Follow the [Next] button to finish the before install the new one.

  • Page 5: Iscapture Parameter Settings

    If get the “No Camera” or “Initialization Failed” error, it could be the camera is not detected by the PC or the driver is not installed properly. Please go to Device Manager to check the camera driver installation status. Normally Auto Exposure function can get proper brightness ISCapture Parameter Settings preview.
  • Page 6 Click the Lock tag to lock/unlock the preview and capture To get better white balance result, please move the sample to the blank area and then hit White Balance button, then move back the resolution. Unlocking it allows to set different preview and capture sample.
  • Page 7 Every time click Capture button , the file save dialog will pop up to ask to set file name, save directory and image format. b. Select Use File Save Dialog to use pop up dialog to set capture image file name, save directory and format.

  • Page 8: Chapter 2: Image Acquisition

    Chapter 2: Image Acquisition Capture tab allows to do parameter settings, capture still images, record videos and live image measurement.

  • Page 9: Basic Control

    Basic Control Note: [Lock WB] ONLY will appear when HD1500MET camera is Provide basic exposure setting, white balance and image resolution attached. settings. NO DRIVER INSTALLATION IS NEEDED WHEN USE HD1500MET.

  • Page 10: Take Still Images And Videos

     [Image Denoise]: checked to do the image noise reduction to Take Still Images and Videos save better quality image. Note: In long time exposure application, it needs to wait quite a while to save an image.  [Combined Measurement]: Checked to save the live measurements to the captured image.

  • Page 11: Capturing And Saving Individual Images

    save name to be pre-set, format, image quantity, capture software will automatically save a set of images after a single interval time and the file save directory. Upon executing [Capture] is executed. [Capture] or [Video] button , ISC will save files as set. ...

  • Page 12: Video Recording

     This set of image file names follows the same way format as set for individual image capturing. If [File Name] is used, image names will be in the form “X”, “X-1”, “X-2”... (where X is the character/s entered or “IS” by default). Video recording Click [Video] , start/ stop video...

  • Page 13: File Save Destination

    compressors on the PC. Exposure Control Note: The video taken without any compression will be very large size. ISC will automatically search the installed video compressors installed on the PC. File Save Destination Click [Browse...] to change the file save destination. The default path is the software installation folder.

  • Page 14: Auto Exposure

    Auto Exposure Manual Exposure  Check [Auto Exposure] checkbox, software start to adjust the exposure time automatically to get proper brightness of live image. Adjust the exposure time manually. Two ways to change the exposure time:  Auto exposure target value: Set the reference exposure time ...

  • Page 15: Gain, Frame Speed & Data Width

    Levels [Update] appears after [Extend] is selected. Click on it to stop the previous exposure time and restart the new one immediately. For long exposure applications, we strongly recommend that [Update] is used to start a new setting. This will help to get the new exposed image earlier.

  • Page 16: Color Control

    [Auto] checkbox: Adjust the live image levels automatically. need to go back to the original image, click restore the image. Manual adjustments of image levels:  Black Point Slider: It is used to set the black point or shadow [Area]: Manually select the dark image area as a reference to optimize the dark field imaging background.

  • Page 17: Flat Fielding Function

    Used for adjusting image color, gamma, contrast and saturation. Flat Fielding Function Flat fielding function is used to correct the uneven background brightness.  Click on [FlatFielding] to start the flat fielding parameter calculation and apply it to the live images. ...

  • Page 18: Fluorescence Mode

    Fluorescence Mode Pseudo Color function This function is ONLY available for CCD monochrome and Discovery MH15 cameras. Apply pseudo color to the live images. Two ways provided for color assignment: a. Click on the preferred color or move the slider to choose it.

  • Page 19: Black Level

    Black Level Fluorescence Wizard Black level function defines the brightness level at the darkest part of the image. In low light imaging, it can help to see more details in the dark area. Note: In low light application, it usually needs quite a long exposure time to get proper images.

  • Page 20: Temperature Control

    Short exposure time will give faster frame rate which will be easier Temperature Control for adjust the focus. High gain make the image bright but also bring significant noise. So it is normal to get noisy image in this step. Step2: When get bright enough images, adjust your microscope (or lens) to make the image in focus.

  • Page 21: Parameter

    image. Parameter  Delete function: Choose a parameter team and click to remove it from the PC.  Backup function: backup all the saved parameter teams. Click to get a pop up dialog which allows to select the parameter setting information save directory. ...

  • Page 22: Measurement List

    Measurement List...

  • Page 23: Edit Scale Line

    Edit Scale Line  Edit scale character  Edit the frame of the scale Double click on the scale to get its properties and make changes to  Edit scale line length and name...

  • Page 24: Create Calibration File

    2. Click to start to create calibration file. Create Calibration File To measure the samples real size, the corresponding calibration file needs to be created first. Take pictures of the calibration slide in all the required working objectives and resolution (if a reducing lens is also used in your application, it also requires you to take the calibration slide 3.
  • Page 25 5. Enter the name for the calibration file and the length of the line 4. Click [Distance scaling] and move the cursor to the slide image, you draw. draw a line to get the reference length. Note: If you need more than one calibration file, using Note: Using longer length as the reference length will give more...

  • Page 26: Calibration Table

    the calibration scale unit is 0.1mm; the Measure Unit is selected as Calibration Table µm; and the reference length is 10 scale units, so the length should be 10 x 0.1mm x 1000 = 1000 µm. 6. Click [OK] to confirm the calibration. The new calibration file named “10X”...

  • Page 27: Measurement List

    useful to name the calibration file with the capturing settings or Measurement objective name. ISC allows you to do line, parallel, perpendicular, rectangle, circle, Measurement List polygon, arc and angle measurement. The [Count] function allows you to manually count the objects. And the [Annotate] function offers to add comments on the images.

  • Page 28: Annotate

    Ruler Click on to show or cancel the cross-line on the images. The Annotate displayed ruler unit is determined by the selected calibration file. Double-click on the ruler to get the ruler property and change the ruler color. The default color is BLACK.

  • Page 29: Layer

    Layer Create multiple layers for loads of measurements. The layer function makes adding a large number of measurements on the processed image review simple and easy. If you have already applied some measurements on the image, the [Layer] function automatically creates “Background” and “Layer01” for the current image.
  • Page 30 Checked [Cur] means the corresponding layer is displayed currently. Click [New] to create a new layer. Allow to key in the preferred Select different [Cur] to switch between different layers. In the name for the new layer. It uses “Layer02”, “Layer03”... etc as the [Visible] column, the selected check box means all the layer name by default.

  • Page 31: Live Image Shortcut

    The layer information is saved in a text file.  Click [Browse] to choose the text file saving directory and enter file name. Then click [Save] to save the current layer information in the text file. The layer information will be saved as “LayerInfo.txt”...

  • Page 32: Chapter 3: Image Management

    Image Management and Processing Shortcuts Chapter 3: Image Management ISC provides some quick functions on the right hand side of the View images in [Browse] panel, it displays the image File name, software in Browse or Image mode. capturing time, color depth (bit), picture resolution and image size. It also allows you to add comments to any individual image.

  • Page 33: Chapter 4: Image Processing Tab

    Image Processing Chapter 4: Image Processing Tab Provide basic still image processing functions. In this section, ISC provides advanced image processing functions and also allows you to do the measurement on the still images.

  • Page 34: Level

    Control]-->>[Levels]. Note: After clicking [Apply], all the settings are applied to the image. Please note , once you choose this you can revert to Measure the original image. Click on [Measure] at the top of the ISC to get the measurement tools.

  • Page 35: Fluorescence

    Step 2: Click on image thumbnails to add corresponding images. Fluorescence The image position indicator shows the added images’ position. Maximum 4 frame images are allowed to add for fluorescence combination. This function is used to assign Black & White fluorescence images Step 3: Click on one added image indicator to start applying color with different colors and combine them together into one image.
  • Page 36 a. Click on the preferred color or slider to choose it. b. Assign the color according to the fluorescence dye in the drop-down menu [Dye Type].  Click on [Apply Color] button to add selected color on the image. Note: Optimize checkbox is recommended to select during the combination.

  • Page 37: Label

    can help to get sharper images and see more image details. If you add the wrong image or wrong color to selected image, just click on the tiny cross beside each indicator to delete it. If you want to cancel the current combination, just click on [Close All] to cancel the combination.

  • Page 38: Class Counting

    image. Class counting function allows to do 5 different types samples counting manually. Each type will be assigned with different color  The date and time will be displayed on the top right corner dots. the image.  After clicking [Save], the image with the label will be saved as image file name+ _bak.

  • Page 39: Advanced Functions

    Advanced Functions Extend DoF (Extend Depth of Focus), Stitching (Image Stitching) and HDR (High Dynamic Range Image). Push [Extend DoF]/[Stitching]/[HDR] to get below dialog box. Select the corresponding images and apply the function. This function combines multiple images to create one focused/ panorama/ high dynamic range image.
  • Page 40 Tel: (877) 877-7274 | Fax: (516) 801-2046 Email: Info@nyscopes.com [Delete] to remove it. [Delete all] will remove all the selected www.microscopeinternational.com images. Meiji Techno America 5895 Rue Ferrari San Jose, CA 95138 Tel: 1(800) 832-0060 Fax: (408) 226-0900 www.meijitechno.com info@meijitechno.com...

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