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Nikon TE2000 Manual page 5

Fluorescent microscope
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The difference between the objectives
Possit
ion
1
2
3
4
CFI Plan Fluor ELWD 40X
5
6
HMC - Hoffman modulation contrast (objectives in position 5-6). The method converts phase
gradients into intensity variations to create 3D-like images, allowing observation in plastic dishes.
These objectives are suited for plastic dishes.
Description
CFI Plan Fluor 4X
CFI Plan Fluor 10X
ELWD 20X
HMC LWD 20X
HMC LWD 40X
Indicates the phase-ring. Use "Ph2" for
this objective.
NA
0,13
0,30
0,40
0,60
0,40
0,55
Indicates the NA. The higher the
number the better the resolution.
Correction collar. You have to adjust
the ring to the coverslip-petridish
thickness. Turn until the image looks
the sharpest.
WD (mm)
Remarks
17,1
16,0
Ph 1
Ph1
3,7-2,7
Ph2
MC 2
MC 3

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