Pipetting Techniques - LABBOX EASY 40 Elite User Manual

4. MICROPIPETTE COMPONENTS
Push b utton
rod
Grip
cove
r
Display
cover
Tip C ollar
5. MICROPIPETTE OPERATION
5.1. Volume setting
The volume of the micropipette is clearly shown through
the handle grip window. The delivery volume is set by
turning the thumb button clockwise is anticlockwise
(Fig.3). When setting the volume, please make sure that:
 The desired delivery volume clicks into place
 The digits are completely visible in the display
window
 The selected volume is within the pipette's
specified range
Using excessive force to turn the push button outside
the range may jam the mechanism and damage the
micropipette.
5.2. Sealing and ejecting tips
Before fitting a tip makes sure that the micropipette
tip cone is clean. Press the tip on the cone of the
micropipette firmly to ensure an airtight seal. The seal
is tight when a visible sealing ring forms between the tip
and the black tip cone (Fig.4).
Each micropipette is fitted with a tip ejector to
help eliminate the safety hazards associated with
contamination. The tip ejector needs to be pressed
firmly downwards to ensure proper tip ejection (Fig.5).
Make sure the tip is disposed of into a suitable waster
container.

6. PIPETTING TECHNIQUES

6.1. Forward pipetting
Make sure that the tip is firmly attached to the tip cone. For best results the thumb
button should be operated slowly and smoothly at all times, particularly with
viscous liquids.
Hold the micropipette vertically during aspiration. Make sure that the liquid and
container vessel are clean and that the pipette, tips and the liquid are at the same
temperature.
1. Depress the thumb button to the first stop (Fig.6B).
2. Place the tip just under the surface of the liquid (2-3mm) and smoothly
release the thumb button. Carefully withdraw the tip from the liquid, touching
against the edge of container to remove excess.
3. Liquid is dispensed by gently depressing the thumb button to the first stop
(Fig.6B). After a short delay continue to depress the thumb button to the
second stop (Fig.6C). This procedure will empty the tip and ensure accurate
delivery.
Push b utton
(Thumb b utton
)
Cap
Tip E jector
Handle
Tip c one
3