Related Manuals for Cairn OptoSplit III
Summary of Contents for Cairn OptoSplit III
- Page 1 Free Phone: 08453301267 (UK only) Tel: +44 (0) 1795590140 Fax: +44 (0) 1795594510...
- Page 4 The OptoSplit is supplied pre-aligned and centred, please refer to this manual before making any adjustments. When changing the Cairn Filter Cube, care should be taken not to touch any optical surfaces. If any lenses, lters, or mirrors are marked...
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Page 5: Table Of Contents
Installation guide Installing Filters into the Cairn Filter Cube Installing Dichroic Mirrors into the Cairn Filter Cube Inserting the Cairn Filter Cube into the Optopsplit III Auxilliary Component Mounts Installing the OptoSplit III in the Lightpath Operation of the OptoSplit III... -
Page 6: Introduction
In conjunction with a research microscope and a suitable CCD camera the Cairn OptoSplit III allows the researcher to do exactly this. The Cairn OptoSplit III is usually supplied with a rectangular aperture to de ne a ROI and includes controls to allow up to three images to be positioned accurately and conveniently within the camera frame. -
Page 7: Installation Guide
2.2. Always take extreme care when changing or adjusting the lter and mirror sets to avoid damage or soiling. If you purchased your OptoSplit III with a full lter set pre-installed, then you may wish to go to section 2.4 as the following may not be pertenant. -
Page 8: Installing Dichroic Mirrors Into The Cairn Filter Cube
Cairn Filter Cube is designed to mate with the bracket on the internal wall of the OptoSplit III, so that the small handle on top of the cube will be facing out. Once the Cairn Filter Cube is mounted successfully, replace the access panel using the two handles. - Page 9 Mounting bracket Remove the access panel using the two handles. Mount the lter cube onto the brackect on the interior of the OptoSplit II. Replace the access panel using the two handles. Page 4...
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Page 10: Auxilliary Component Mounts
Auxilliary component mounts. Installing the OptoSplit III in the Light Path Before installing the OptoSplit III unit it is important to rst set up the microscope, camera, and software to give a clear image of an object of less than half the size the camera frame. Ideally this should be a real sample with the appropriate optical properties for the installed lter set. -
Page 11: Operation Of The Optosplit
Operation of the OptoSplit III The OptoSplit III uses a single control for adjusting image separation and allows for di erent sizes of samples to be used. There are additional controls for re ning the ROI, and centering the image. -
Page 12: Adjusting And Locking The Aperture
In order to adjust the ROI, adjust each of the two levers on the input port of the OptoSplit III until the desired area is de ned on the camera. If you wish to lock the aperture in place, then tighten the levers by rotating them clockwise, and to loosen them rotate anti-clockwise. - Page 13 Centre image will not deviate. Note: To prevent calibration of the Transmitted Centre Re ected OptoSplit III o -axis, the Re ected (or image image image short wavelength) image has no independent horizontal controls, so it is...
- Page 14 Turning the V1 controller will adjust the vertical position of the Re ected, and the Centre images, the remaining image is the Transmitted image which should be on the left side, if it is on the right, then adjust the Split control until the outside images are exchanged Adjustments to the V2 controller will move the Centre image along the...
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Page 15: Focussing The Camera On The Aperture
Split control If the OptoSplit III aperture is not in sharp focus, then adjust the ne focus on the camera as follows: Set a convenient sized aperture using the input aperture levers so the edges of the aperture are within the eld of view. -
Page 16: Dual And Single Image Modes Of Use
OptoSplit III eld aperture. Dual and Single Image Modes of Use The OptoSplit III can be setup in single channel or two channel (OptoSplit) modes, by simply exchanging the lter cube & adjusting the eld aperture. Single image or “bypass” mode is the simplest of these, only requiring the eld diaphragm to be fully opened to enable operation in full frame mode. -
Page 17: Locating The Optosplit Images On The Camera
Locating the OptoSplit images on the camera If the OptoSplit III controls have been badly misaligned, the images can show signi cant aberrations, or in extreme cases only a single image may be observable for any setting of the ‘Split’ control. In these cases the following procedure will enable the device setup to be recon g- ured. - Page 18 plates provided. De ne a small area using the OptoSplit input aperture diaphragm and ensure the input to the OptoSplit is illuminated. Attempt to centre the re ected image pathway horizontally using the ‘Split’ control to scan the position range. If no aperture edges can be located for any position of the ‘Split’control, enlarge the input aperture and repeat step 3.
- Page 19 Locating the Centre Image Move the shutter from the centre channel to the transmitted illumination pathway, the calibration cube should remain in place. With the re ected & transmitted images located, it is likely the centre image will be seen somewhere in the eld of view.
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Page 20: Image Optimisation (Trim Adjustment)
It is important to remember to re-tighten the clamp screw once the adjustment is complete. If the OptoSplit III has been con gured into single channel (non-split) mode and Trim adjustment is required to compensate for vignetting being observed, it is likely the slider will need to be at the extreme of its range of movement to either end. -
Page 21: Technical Summary
Technical Summary The OptoSplit III uses two conventional dichroic mirrors to seperate the light into three di erent spectral bands. This wavelength selection is usually augmented by the use of bandpass lters as shown below. Angled Mirrors Aperture Direction of light... -
Page 22: Technical Support
Technical Support E-mail: tech@cairn-research.co.uk Web: http://www.cairn-research.co.uk Address: Cairn Research Graveney Road Faversham Kent ME13 8UP Telephone: +44 (0) 1795590140 Fax: +44 (0) 1795594510 Page 17... - Page 23 In addition to designing and manufacturing our own products, we are also proud distributors for the following companies Page 18...