Cairn TWINCAM Quick Start

TWINCAM
VERSION: MAY 2017 (SN 7039 - PRESENT)
The TwinCam is a simple, yet powerful and stable device for pixel aligning images captured using different wavelengths,
polarization states, fields of view or other imaging modalities onto two camera sensors. It is designed to meet the most
demanding requirements for the sub-pixel mapping of Super Resolution imaging and is compatible with most scientific
C mount and F mount cameras.
Please contact us if you require any assistance during setup or use (Tech@cairn-research.co.uk).
For the purpose of this Quick Start Guide, please refer to the TwinCam diagram on Page 3. All words in
labelled on this diagram.
A) Mounting to your microscope
1. Connect the TwinCam to your existing 1x camera c-mount (or confocal unit if appropriate).
2. If using an inverted microscope, two support jacks are normally supplied to support the weight of the unit. Position
the support jacks in the
spirit level
locking collar. A
C-mount camera tube
3. Remove the
2.5mm allen key provided).
4. Screw each camera onto the
still in place.
B) Aligning two images
1. View a live image (ideally of a graticule using transmitted light) on the
to be able to view all four edges of the
this stage.
rectangular aperture
2. Close the
clamping
3. Lock off the
4. Insert the calibration cube (containing a 50% mirror) into the unit by removing the magnetic
ensure the cube locates correctly.
5. Repeat steps 1 to 3 on the
6. If the sample focus of the reflected camera is not precisely matching the focus of the transmitted camera, an adjust-
ment is provided (to allow for any slight differences between positioning of the camera sensor). Remove the
ring
on the reflected port and move the camera in Z until focused. Lock off the
Each of the two images can now be overlaid in your imaging software and pixel aligned using two sets of XY controls:
7. With the rectangular aperture
transmitted H&V
adjusting the
your imaging software (if available).
8. Centre the reflected port using the
9. Note the reflected image will be mirrored horizontally, which will need to be flipped in your imaging software.
10. Replace the calibration cube with a filter cube for dual channel imaging (see section C).
rectangular aperture
11. Open the
jacking point
on each output. The height can be adjusted and locked in position with the blue
is also included in the input section to aid setup.
dust
and protective
C-mount camera tube
rectangular
until in view on the image and rotate the camera until square.
screw.
reflected port.
still in view, centre the transmitted port (using the 1.5mm allen key provided) by
controls. It's useful to use a centred square or central crosshair as a reference point in
reflected H&V controls.
to slightly larger than the camera field of view to minimize scattered light.
email: sales@cairn-research.co.uk tech@cairn-research.co.uk
+44(0)1795 590140 www.cairn-research.co.uk
cap. The clamping screw
and replace on each arm of the TwinCam with the
transmitted
aperture, therefore it is not advisable to use a fluorescent sample at
clamping screw
Q u i c k S t a r t
bold blue
text are
may need to be loosened (using the
calibration ring
port. For initial setup, it is essential
door cover. Magnets will
calibration
once again.