Oxford Instruments Andor Sona 4.2B-11 Hardware Manual
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Oxford Instruments Andor Sona 4.2B-11 Hardware Manual

Back-illuminated scmos camera
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Sona & Marana
Version 1.5 rev 4 Dec 2019
Hardware Guide
for Sona 4.2B-11, 2.0B-11
and Marana 4.2B-11 Models
andor.com
© Andor Technology Ltd. 2019.

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Summary of Contents for Oxford Instruments Andor Sona 4.2B-11

  • Page 1 Sona & Marana Version 1.5 rev 4 Dec 2019 Hardware Guide for Sona 4.2B-11, 2.0B-11 and Marana 4.2B-11 Models andor.com © Andor Technology Ltd. 2019.

  • Page 2: Table Of Contents

    Sona & Marana TABLE OF CONTENTS SECTION 1: INTRODUCTION ......................10 TECHNICAL SUPPORT ......................11 DISCLAIMER .......................... 12 COPYRIGHT AND PROTECTIVE NOTICES ................12 TRADEMARKS AND PATENT INFORMATION ............... 12 SUPPLIED COMPONENTS ....................13 1.5.1 ....................14 amera odel ptions 1.5.2 ....................
  • Page 3 Sona & Marana CONNECTING THE LIQUID COOLING SYSTEM ..............24 3.9.1 ................... 24 onneCting the oolant oses 3.9.2 ................24 isConneCting the oolant oses 3.10 INSTALLING SOFTWARE AND USB DRIVERS ..............25 3.10.1 m ................25 inimum omputer equirements 3.10.2 i usB d ..............
  • Page 4 Sona & Marana SECTION 5: MAINTENANCE ......................41 REGULAR CHECKS ....................... 41 ANNUAL ELECTRICAL SAFETY CHECKS ................41 GENERAL CLEANING & DECONTAMINATION INFORMATION ..........41 CLEANING THE CAMERA WINDOW ..................41 5.4.1 ......................42 ools equired 5.4.2 ..................42 indow leaning roCedure FUSE REPLACEMENT ......................
  • Page 5 Sona & Marana APPENDIX A: TECHNICAL SPECIFICATIONS ................... 45 APPENDIX B: MECHANICAL DRAWINGS ..................48 APPENDIX C: DEWPOINT INFORMATION ..................49 APPENDIX D: REFERENCE INFORMATION ..................50 APPENDIX E: OTHER INFORMATION ....................51 version 1.5 rev 4 Dec 2019...
  • Page 6 Sona & Marana evision istoRy Version Released Description Initial Release 04 Sep 2018 11 Apr 2019 Updated USA and Asia-Pacific addresses Updated altitude information on page 46, updated backplate images and 19 Jun 2019 mechanical drawings for Marana. USB terminology amended throughout. Updated China office address, table on page 39 and power supply information.
  • Page 7 Sona & Marana afety and aRning nfoRMation READ THIS INFORMATION FIRST If the equipment is used in a manner not specified by Andor, the protection provided by the equipment may be impaired. Before using the system, please follow and adhere to all warnings, safety, manual handling and operating instructions located either on the product or in this Hardware Guide.
  • Page 8 Sona & Marana 20. If any ingress of liquids has occurred or is suspected, unplug the mains cables, do not use, and contact Andor Customer Support. 21. When using a liquid cooling system it is recommended that an overpressure device is fitted to avoid leaks that may find their way to the mains electricity supply and create a hazard.
  • Page 9 Sona & Marana aRning and afety abels The following warning labels appear on the product: There are no user serviceable components inside. The serial label of the camera (above) is located on the back of the camera. It shows the product model, serial number and the build date.

  • Page 10: Section 1: Introduction

    Sona & Marana SECTION 1: INTRODUCTION This manual provides an overview of the Sona and Marana camera series. The Sona and Marana feature the latest back-illuminated sCMOS sensor technology for an exceptional sensitivity, speed and field of view. This makes it particularly suitable for demanding applications - Sona for life science imaging: such as live-cell imaging, embryo imaging;...

  • Page 11: Technical Support

    Sona & Marana 1.1 t ecHnical UppoRt If you have any questions regarding the use of this equipment, please contact the representative* from whom your system was purchased, or: Europe Andor Technology Ltd. Andor Technology 7 Millennium Way 300 Baker Avenue Springvale Business Park Suite # 150 Belfast...

  • Page 12: Disclaimer

    RadeMaRks and atent nfoRMation Andor and the Andor logo are trademarks of Andor Technology Ltd. Andor Technology Ltd. is an Oxford Instruments company. All other marks are property of their owners. Manufacturers Information Andor Technology Ltd., Belfast, BT12 7AL, UK.

  • Page 13: Supplied Components

    Sona & Marana 1.5 s Upplied oMponents Description Quantity Sona or Marana back illuminated sCMOS Camera (model as ordered) Software & Driver Hardware Guide in CDs or Flash Drive electronic format (Solis is ordered separately) Power Supply Power Cord (Country specific) 1 x 15 V USB 3 Card Quick Start Guide...

  • Page 14: C Amera M Odel O Ptions

    Sona & Marana 1.5.1 c aMeRa odel ptions There are a number of models of Sona and Marana camera based off of a common architecture and shared design (see also Section 2.1). The differences for each model can be identified from the product codes and descriptions outlined below: Model Type Product Code...

  • Page 15: Section 2: Product Overview

    Sona & Marana SECTION 2: PRODUCT OVERVIEW This section provides an overview of the Sona and Marana. Please note that Sona configuration is shown below which will also cover the Marana model. Please refer to the additional information supplied for details of any model specific differences.

  • Page 16: Rear Panel

    Sona & Marana 2.2 R anel External I/O ON/Off Power Input Connection Switch USB 3 Type-B port Water Connections: Compatible with tailpipes for 1/4” bore soft PVC hose (2 plcs) Figure 3: Rear Panel USB Connectivity USB 3 connection provides a robust high speed connection to the control PC. External I/O: TTL / Logic The TTL/Logic connection permits connection to other devices for synchronisation and control of fire, trigger and shutter operations.

  • Page 17: Power Requirements

    Sona & Marana Ext Trigger (and Spare*)Inputs Camera 3.3V (5 V tolerant) > 2.4V Logic level 1 Input triggers on positive edge < 0.8V Logic level 0 10K Ohms Arm (Fire*, Fire n*) and AUX_OUT Outputs Camera > 2.0V > 1K Ohms 10K Ohms <...

  • Page 18: Section 3: Installation

    Sona & Marana SECTION 3: INSTALLATION WARNINGS: • PRIOR TO COMMENCING INSTALLATION, THE USER SHOULD REFER TO THE SAFETY AND WARNING INFORMATION AND UNPACKING INSTRUCTIONS AT THE BEGINNING OF THIS MANUAL. • DUE CARE MUST BE TAKEN WHEN LIFTING THE CAMERA. ENSURE THAT THE MOUNTING AND CONNECTED ASSEMBLY IS SECURE AND ABLE TO SUPPORT THE WEIGHT OF THE CAMERA.

  • Page 19: Mount And Mount Daptors

    Sona & Marana 3.2.2 f- MoUnt and MoUnt daptoRs Note: F-Mount and T-mounts- Use the inner set of mounting points (shown in section 3.2.1). Removing the F/T-mount 1. Place the camera on its side (to prevent debris and dust landing on the window). 2.

  • Page 20: Connecting The Camera To The Pc

    Sona & Marana 3.5 c onnecting tHe aMeRa to tHe The camera connects to a PC via USB 3 this provides a standard, robust and high speed connection with the control PC. It is recommended to use the supplied USB 3 card as this will ensure consistent performance. Other USB ports on the PC may share bandwidth with other devices and peripheral components.

  • Page 21: C Onnecting The C Amera To The Pci E C Ard

    Sona & Marana 3.5.2 c onnecting tHe aMeRa to tHe • Connect the USB cable from the camera to the appropriate PCIe card on the control PC. 3.6 c onnecting tHe aMeRa to tHe oWeR Upply • Connect the mains power cable between the camera power input (15 V DC) and the 15 V DC Power Supply Unit.

  • Page 22: Connecting A Cooling System

    Sona & Marana 3.7 c onnecting a ooling ysteM The camera can use either air cooling, or optional liquid cooling for deeper cooling. 3.7.1 i MpoRtant onsideRations WHen Using iqUid ooling ysteMs • Before attempting to insert or remove the coolant hose connections, ensure that all coolant has been drained from the hoses and integral coolant channel within the camera head.

  • Page 23: Coolant Recommendations

    Sona & Marana Hose inserts are provided to enable connection to coolant hoses. • Coolant Hose Inserts: Two barbed coolant hose inserts (replacement part ) are supplied as 6MM-HOSE-BARBS standard, suitable for connection to 6 mm [0.25”] internal diameter soft PVC tubing / hose. •...

  • Page 24: Connecting The Liquid Cooling System

    Sona & Marana 3.9 c onnecting tHe iqUid ooling ysteM An overview for connecting a liquid cooling system is outlined below- please refer to the information supplied with your cooling system for further information. 3.9.1 c onnecting tHe oolant oses 1.

  • Page 25: 3.10 Installing Software And Usb Drivers

    Sona & Marana 3.10 INSTALLING SOFTWARE AND USB DRIVERS 3.10.1 M iniMUM oMpUteR eqUiReMents • 3.0 GHz single core or 2.4 GHz dual or quad core processor • 4 GB RAM • Hard drive: 400MB/sec write speed recommended for the data rate associated with the max. frame rates. 100 MB free hard disc to install software •...

  • Page 26: Nstalling The Usb

    Sona & Marana 3.10.4 i Usb 3 (pci nstalling tHe RiveRs • The drivers for the PCIe card must be installed manually by the user as they are not part of the SDK installation. Refer to the instructions on the driver CD supplied with the USB 3 Card. •...

  • Page 27: Section 4: Operation

    Sona & Marana SECTION 4: OPERATION WARNINGS: • IF THE EQUIPMENT IS USED IN A MANNER NOT SPECIFIED BY ANDOR OR ITS DISTRIBUTORS, THE PROTECTION PROVIDED BY THE EQUIPMENT MAY BE IMPAIRED. • PLEASE READ THE USER GUIDES SUPPLIED WITH YOUR SYSTEM COMPONENTS AND CAMERA CONTROL SOFTWARE PRIOR TO USE.

  • Page 28: E Xtended D Ynamic R Ange (Edr)

    Sona & Marana Light (photons) hits the sensor and generates charge (electrons). The photo-generated charge is converted to an analog voltage for each pixel amplifier. These pixel voltages are transferred to the column bus via a row select signal. The analog voltages are then converted to digital signals via columns of analog to digital (A/D) converters. The final digitized signals are then read out sequentially at a pixel readout speed of up to 200 MHz.

  • Page 29: U Singing Roi Sing (Aoi Sing )

    Sona & Marana 4.4.3 U (aoi sing Region of Interest (ROI) also called Area of Interest (AOI) can be selected so that only a defined region of the sensor is used. This smaller “cropped” region of the sensor can subsequently be read out much faster than the full sensor area so that frame rates may be significantly higher (see the preceding table).

  • Page 30: B Lemish C Orrection F Ilter

    Sona & Marana 4.4.6 b leMisH oRRection ilteR The Blemish Correction Filter identifies and compensates for three types of blemishes during the FPGA processing step: Hot Pixels Noisy Pixels Unresponsive Pixels sCMOS sensors are particularly susceptible to hot pixel blemishes. These are spurious noise pixels that have significantly higher darkcurrent than the average.

  • Page 31: Software Acquisition Events

    Sona & Marana 4.6 s oftWaRe cqUisition vents Software Acquisition Events are only accessible via SDK - these are not available in Solis, Fusion, iQ or other software but may be used internally. Refer to the SDK3 manual for further information on configuration of Software Acquisition Events.

  • Page 32: Hutter Olling S Huttehutter Echanism Echanisechanism

    Sona & Marana camcorders were the image readout rate was not fast enough to keep up with a panned image. In modern sCMOS cameras however, the readout speeds are so high that rolling shutter is most suited for the majority of scientific applications.

  • Page 33: T Iming P Arameters And E Xternal T Riggering

    Sona & Marana 4.7.3 t iMing aRaMeteRs and xteRnal RiggeRing The timing tables accompanying each of the triggering diagrams that follow indicate the exposure and cycle times achievable in each triggering mode. These are based on Frame and Row Periods as shown below. Timing Parameters based on Sensor Clock Speed: 1 Row 20.52 μs...
  • Page 34 Sona & Marana Figure 8: Internal triggering “long” (non-overlap) Parameter Minimum Maximum Exposure 1 Row 30 s Cycle Time (1/Frame Rate) Exposure + 1 Frame + 1 Row 44,000 s Note: actual minimum Exposure time is 15 μs in 12-bit mode and 9.48 μs in 16-bit mode. The exposure time is incremented in 1 Row time steps.

  • Page 35: R Olling S Hutter I Nternal T Riggering (O Verlap M Ode )

    Sona & Marana 4.7.5 R olling HUtteR nteRnal RiggeRing veRlap Internal Triggering in Overlap Mode allows the user to perform an exposure and acquire images from the sensor simultaneously. This is achieved by starting a new exposure for a new frame while the current frame’s exposure is being read out from the sensor.

  • Page 36: R Olling S Hutter E Xternal / S Oftware T Riggering (N On -O Verlap M Ode )

    Sona & Marana Parameter Minimum Maximum Exposure 1 Row 30 s Greater of: Cycle Time (1/Frame Rate) 1 Frame + 1 Row or Exposure time + 1 Row FIRE Any low period ~5 μs in 12-bit and ~10 μs in 16-bit (1 Row) 4.7.6 R olling HUtteR...
  • Page 37 Sona & Marana Figure 12: External Triggering “long” (non-overlap) Parameter Minimum Maximum Exposure 3 Rows 30 s Cycle Time (1/Frame Rate) Exposure + 1 Frame + 1 Row External Start Delay 1 Row EXT Trig Pulse Width 6.6 ns version 1.5 rev 4 Dec 2019...

  • Page 38: Olling Hutter Xternal Xposure Riggering On Verlap Ode )

    Sona & Marana 4.7.7 R olling HUtteR xteRnal xposURe RiggeRing veRlap On detection of the trigger event a reset read out is initiated, this effectively begins a new exposure. When the external trigger input goes LOW, a signal frame read out phase begins. When the frame has been read out completely, the Arm goes high and the camera waits for the next trigger event to be detected.

  • Page 39: Olling Hutter Xternal Xposure Riggering Verlap Ode )

    Sona & Marana 4.7.8 R olling HUtteR xteRnal xposURe RiggeRing veRlap In overlap mode, every positive edge of an external trigger will trigger a frame read out and start a new exposure for the next frame. The period of external trigger pulse defines exposure and cycle time for each frame read out. On detection of the positive edge a frame read out is initiated at the start of the next Row read period.

  • Page 40: Hutarttarter Olling S Hutarttartehutarttarter E Xtartehutarttarternal S Tartahutarttartertart Tart Hutarttarteriggehutarttartering

    Sona & Marana 4.7.9 R olling HUtteR xteRnal taRt RiggeRing In this mode the camera will wait for a single external trigger event. Once this external trigger event is detected, the camera will progress as if the camera was in internal trigger mode (see Section 4.7.4 and 4.7.5). The ARM signal indicates to the user when the camera is ready to detect an External Start Trigger.
  • Page 41 Sona & Marana SECTION 5: MAINTENANCE THERE ARE NO USER-SERVICEABLE PARTS INSIDE THE CAMERA. DAMAGE CAUSED BY UNAUTHORISED MAINTENANCE OR PROCEDURES WILL INVALIDATE THE WARRANTY. 5.1 R egUlaR Hecks • The state of the product should be checked regularly, especially the integrity of the PSU and the mains cable. •...
  • Page 42 Sona & Marana 5.4.1 t ools eqUiRed • Compressed Air Can (or source of clean compressed air) • Optics Brush 5.4.2 W indoW leaning RocedURe Remove the camera from your microscope (or other optical equipment) and place it on a clean dry surface. Use the camera control software to open the shutter (if fitted).
  • Page 43 Sona & Marana SECTION 6: TROUBLESHOOTING 6.1 p Reventing ondensation Key Risks • Take special care during installation as the temperature of the camera may be low from shipping or storage. When moved to a warmer environment such as a lab, there is a higher risk of condensation forming. Therefore, ensure that sufficient time is allowed for the product to reach the ambient temperature of the operating environment before use (this may take several hours).
  • Page 44 Sona & Marana 6.2 q Uick RoUblesHooting Uide Issue Possible Cause Action Camera buzzer does not sound on start-up Communication error. Ensure that power is connected to the camera and the On/Off switch is set to On Camera is not recognized by PC Camera not switched on.

  • Page 45: Appendix A: Technical Specifications

    Sona & Marana APPENDIX A: TECHNICAL SPECIFICATIONS Performance Specifications •1 Model Sona and Marana 4.2B-11 Sona 2.0B-11 Sensor Type Back-Illuminated Scientific CMOS 2048 (W) x 2048 (H) 1400 (W) x 1400 (H) Array Size 4.2 Megapixel 2.0 Megapixel Pixel Size 11 x 11 22.5 mm x 22.5 mm 13.2 mm x 13.2 mm...
  • Page 46 Sona & Marana General Specifications •1 Model Sona and Marana 4.2B-11 Sona 2.0B-11 O: Fire Row 1, Fire Row n, Fire All, Fire Any, Arm I: External Trigger Modes Internal, External, External Start, External Exposure, Software Software Exposure Events Start exposure - End exposure (row 1), Start exposure - End exposure (row n) •5 Hardware Timestamp Accuracy 25 ns...
  • Page 47 Sona & Marana External Power Supply Requirements Low Voltage Supply Input 15 V +/- 5% Low Voltage Supply Current Right-angle Plug: Fischer WSO 104 A037-130+ Low Voltage Supply Cable Connector Straight Plug: Fischer S 104 A037-130+ Required Cable Clamp Set: Fischer E3 104.3/6.7+B Pins 1 &...

  • Page 48: Appendix B: Mechanical Drawings

    Sona & Marana APPENDIX B: MECHANICAL DRAWINGS Sona Marana column 1 row 1 pixel 1, 1 Focal Distance Weight: approx. 2.7 kg [5.95 lbs] version 1.5 rev 4 Dec 2019...

  • Page 49: Appendix C: Dewpoint Information

    Sona & Marana APPENDIX C: DEWPOINT INFORMATION To avoid issues with condensation, the coolant temperature must be set above the dewpoint- the temperature at which condensation (dew) will form. In the relatively dry conditions of an air conditioned lab, or a cool dry climate, use of a coolant temperature of 10 C should not cause any problems.

  • Page 50: Appendix D: Reference Information

    Sona & Marana APPENDIX D: REFERENCE INFORMATION ltRa ecHnology UltraVac is Andors proprietary vacuum technology that provides a permanent, hermetically sealed enclosure (without O-rings) for the sensor. This ensures maximum cooling performance, with a reliability proven through years of use in Andor cameras such as the iXon EMCCD, iKon and Newton series- the Mean Time Between Failure (MTBF) value is >...

  • Page 51: Appendix E: Other Information

    Sona & Marana APPENDIX E: OTHER INFORMATION erms and ondiTions of ale and arranTy nformaTion The terms and conditions of sale, including warranty conditions, will have been made available during the ordering process. The current version may be viewed at: www.andor.com/pdfs/literature/Andor_Standard_Warranty.pdf 2006 (Weee) asTe...

This manual is also suitable for:

Andor sona 2.0b-11Andor marana 4.2b-11Sona-4bv11Sona-2bv11Marana-4bv11Marana-4bu11

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