TABLE OF CONTENTS Overview Features Components Directions for use Casting a gel Running a gel Document the run Troubleshooting and maintenance Troubleshooting Care & maintenance Specifications & operating conditions support@minipcr.com bluegel user’s guide...
Intuitive two-button operation: one for Run, one for Light. 3 x 9 inch footprint, 4 inches high. Storage pouch included. Fold-a-View™ Documentation Hood A portable, foldable darkroom. Image capture even in brightly lit rooms. CE Conformity Mark indicates CE certification. support@minipcr.com bluegel user’s guide...
Let agarose/buffer mix cool for ~2-3 minutes and add 2 μl of GelGreen DNA stain 10,000X stock (1 μl per 10 ml of TBE). Swirl well to mix. See Appendix B for additional DNA staining dyes that work with blueGel support@minipcr.com bluegel...
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DNA gel stains differ in stability and may fade if gels are stored – refer to the manufacturers’ recommendations. support@minipcr.com bluegel user’s guide...
Wipe gently, do not rub clean. 5 -- Place the orange cover on the blueGel base. The cover contains the electrodes and will only fit in one direction, with the (+) electrode positioned to attract the negatively charged DNA.
Pop up the Fold-a-View following the instructions on its side and place it on the blueGel orange cover, sliding it down until it fits snugly around the cover’s edges. Place your camera on top, and align the camera...
Gel edges shrinking after prolonged runs: ensure you are using 0.5X or 1X TBE buffer. If you need to contact miniPCR bio: Phone: 781-990-8PCR Email: support@minipcr.com Mail: 1770 Massachusetts Ave., Suite 167 Cambridge, MA 02140 support@minipcr.com...
CARE & MAINTENANCE OF YOUR BLUEGEL ELECTROPHORESIS SYSTEM Please follow these recommendations to maintain your blueGel system in optimal working condition: Rinse the casting platform, combs, gel tray, buffer chamber and cover in distilled water after each use. Do not wipe or handle platinum wire. Air dry.
Input voltage 100-240V AC~47-63Hz, 0.58A Max Output 48V DC 0.5A 24W Max Indoor use only Operating temperature 9°C - 30°C, max. humidity 70% Appendix A – blueGel™ accessories and replacement parts available at www.minipcr.com Description Part No. GelGreen® Agarose Tabs™...
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