minipcr blueGel User Manual

minipcr blueGel User Manual

Electrophoresis system

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blueGel
Electrophoresis
System
User's guide
Integrated electrophoresis and visualization system
©2013-2020 Amplyus LLC.
miniPCR bio and the miniPCR bio logo
are trademarks of Amplyus LLC
TM
bluegel
TM
user's guide
support@minipcr.com

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Summary of Contents for minipcr blueGel

  • Page 1 Electrophoresis System User’s guide Integrated electrophoresis and visualization system ©2013-2020 Amplyus LLC. miniPCR bio and the miniPCR bio logo are trademarks of Amplyus LLC bluegel user’s guide support@minipcr.com...
  • Page 2: Table Of Contents

    TABLE OF CONTENTS Overview Features Components Directions for use Casting a gel Running a gel Document the run Troubleshooting and maintenance Troubleshooting Care & maintenance Specifications & operating conditions support@minipcr.com bluegel user’s guide...
  • Page 3: Overview

    Intuitive two-button operation: one for Run, one for Light. 3 x 9 inch footprint, 4 inches high. Storage pouch included. Fold-a-View™ Documentation Hood A portable, foldable darkroom. Image capture even in brightly lit rooms. CE Conformity Mark indicates CE certification. support@minipcr.com bluegel user’s guide...
  • Page 4: Components

    COMPONENTS Cover Gel Tray Buffer Chamber Base support@minipcr.com bluegel user’s guide...
  • Page 5 COMPONENTS (Cont.) Combs Gel Tray Casting platform support@minipcr.com bluegel user’s guide...
  • Page 6 Comb storage under casting platform System includes (1) Cover (1) Power supply (2) Gel trays (1) ClearView Spray (1) Buffer chamber (1) Lens cleaning cloth (1) Base (1) Fold-a-View Imaging Hood (1) Casting platform (1) Carrying pouch (2) Two-sided combs support@minipcr.com bluegel user’s guide...
  • Page 7: Directions For Use

    Let agarose/buffer mix cool for ~2-3 minutes and add 2 μl of GelGreen DNA stain 10,000X stock (1 μl per 10 ml of TBE). Swirl well to mix. See Appendix B for additional DNA staining dyes that work with blueGel support@minipcr.com bluegel...
  • Page 8 DNA gel stains differ in stability and may fade if gels are stored – refer to the manufacturers’ recommendations. support@minipcr.com bluegel user’s guide...
  • Page 9: Running A Gel

    Wipe gently, do not rub clean. 5 -- Place the orange cover on the blueGel base. The cover contains the electrodes and will only fit in one direction, with the (+) electrode positioned to attract the negatively charged DNA.
  • Page 10: Document The Run

    Pop up the Fold-a-View following the instructions on its side and place it on the blueGel orange cover, sliding it down until it fits snugly around the cover’s edges. Place your camera on top, and align the camera...
  • Page 11: Troubleshooting And Maintenance

    Gel edges shrinking after prolonged runs: ensure you are using 0.5X or 1X TBE buffer. If you need to contact miniPCR bio: Phone: 781-990-8PCR Email: support@minipcr.com Mail: 1770 Massachusetts Ave., Suite 167 Cambridge, MA 02140 support@minipcr.com...
  • Page 12: Care & Maintenance

    CARE & MAINTENANCE OF YOUR BLUEGEL ELECTROPHORESIS SYSTEM Please follow these recommendations to maintain your blueGel system in optimal working condition: Rinse the casting platform, combs, gel tray, buffer chamber and cover in distilled water after each use. Do not wipe or handle platinum wire. Air dry.
  • Page 13: Specifications & Operating Conditions

    Input voltage 100-240V AC~47-63Hz, 0.58A Max Output 48V DC 0.5A 24W Max Indoor use only Operating temperature 9°C - 30°C, max. humidity 70% Appendix A – blueGel™ accessories and replacement parts available at www.minipcr.com Description Part No. GelGreen® Agarose Tabs™...

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