Dolomite MITOS P-PUMP Series User Manual
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Dolomite MITOS P-PUMP Series User Manual

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MITOS P-PUMP RANGE
USER MANUAL

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Summary of Contents for Dolomite MITOS P-PUMP Series

  • Page 1 MITOS P-PUMP RANGE USER MANUAL...
  • Page 2 Mitos P-Pump Remote range (0 - 10bar) with excellent response time (Part No. 3200176) and accuracy. The design allows users to connect to Dolomite Remote Chambers or custom chambers with a push-click action. The Mitos P-Pump Remote Basic connects to Mitos P-Pump...
  • Page 3 After being used with acetone, the chamber lid should be removed to allow the pressure chamber to be ventilated. Acetone must never be stored in the Mitos P- Pump. For more information on the Mitos P-Pump product range please visit our website http://www.dolomite-microfluidics.com/webshop and download our technical datasheets.
  • Page 4 The key benefits of closed-loop flow control are: • Control flow rates instead of pressure making experiments easier to set-up and optimise. • Improved flow rate precision: even better control over introduction of micro-scale reagent volumes. • Ideal for droplet generation: higher long-term droplet monodispersity in flow control mode compared with pressure control mode.

  • Page 5: Table Of Contents

    Table of Contents Important Information Getting Started Understanding Pressure Driven Flow The Basics General Theory Mitos P-Pump (Part No. 3200016) Features The following accessories are supplied with the Mitos P-Pump: User to supply the following: Fluidic Connection 4.2.1 Opening Chamber 4.2.2 Inserting Fluid Vial 4.2.3...
  • Page 6 11.2 Using the PC software 12 Using Flow Resistors 12.1 Flow Resistors 12.2 Flow Rate Calibration 13 Accessories Dolomite offers a broad range of accessories including flow resistors, vessel holders and starter kits. 13.1 Accessories compatibility SHPT-487168127-329_v.2.0 Page 6 of 60...

  • Page 7: Important Information

    If the glass pressure chamber is scratched or cracked, do not continue to use the Mitos P-Pump. This may result in failure of the Mitos P-Pump and injury to the user. Please contact Dolomite for replacement of the glass vessel.

  • Page 8: Getting Started

    2 Getting Started atmospheric pressure liquid flow liquid flow chamber pressure The Mitos P-Pump range delivers fluid by a pressure driven pumping mechanism. The user sets the chamber pressure (gauge pressure), which determines the flow rate of liquid from the chamber. When the user vents the chamber (or when the Mitos P-Pump range is switched off) the pressure inside the chamber returns to atmospheric and flow stops.

  • Page 9: Understanding Pressure Driven Flow

    3 Understanding Pressure Driven Flow The pressure driven flow concept applies to all pumps within the Mitos P-Pump range, whether the pressure chamber is part of the pump module, i.e Mitos P-Pump and Mitos P-Pump Basic or is connected to the pump via a pneumatic connection, i.e Mitos P-Pump Remote and Mitos P-Pump Remote Basic.

  • Page 10: General Theory

    The following table show the effect of tube length, tube bore and viscosity on flow rate. Effect of tube length Effect of tube Effect of viscosity on flow rate on flow rate internal diameter on flow rate Tube Flow rate Tube ID Flow rate Viscosity...
  • Page 11 The flow resistance of a length of tube (or circular channel) is a function of the internal diameter and length. A long tube with small internal diameter gives a high flow resistance. This can be calculated with the following formula and used to calculate the flow rate in the system when pumping at a selected pressure.

  • Page 12: Mitos P-Pump (Part No. 3200016)

    4 Mitos P-Pump (Part No. 3200016) 4.1 Features Flow sensor module dock Dip tube (liquid outlet) Pressure chamber lid Glass pressure chamber Display screen Twist and click control knob Cancel button Pressure supply connection Mitos Sensor Units Power supply electrical connection connection USB B connection ON / OFF switch...

  • Page 13: The Following Accessories Are Supplied With The Mitos P-Pump

    The following accessories are supplied with the Mitos P-Pump: • Pneumatic supply cable • Tubing FEP 250 µ m ID, 1/16” OD, 2m length • Power supply unit • Mains IEC lead User to supply the following: • Vessel containing sample liquid o Liquids to be pumped must always be placed in a suitable vial within the glass chamber.

  • Page 14: Inserting Fluid Vial

    4.2.2 Inserting Fluid Vial The fluid to be pumped must always be placed inside a small vial and not directly into the chamber! Insert fluid vial Connect dip tube Guide dip tube into vial • • • insert 1/16” OD tube into place fluid vial (smaller than as the chamber lid is put in Ø30 x H88mm) into chamber...

  • Page 15: Adjusting Dip Tube (Part No.3200143)

    4.2.4 Adjusting Dip Tube (Part No.3200143) UNSAFE SAFE TIGHTEN Adjust dip tube Dip tube free (unsafe) Dip tube retained (safe) • • • ensure dip tube is below in this state the dip-tube may to ensure safety, retain dip surface of the liquid be ejected at high pressures tube by inserting into cleat 4.3 Pneumatic Connection...

  • Page 16: Power Connection

    Mitos P-Pump range via a fluidic connection for • use electrical cable supplied with the Mitos more information please see section 8. Sensor Unit For more information on Dolomite’s Mitos Sensor Units please visit our website www.dolomite-microfluidics.com/webshop SHPT-487168127-329_v.2.0 Page 16 of 60...

  • Page 17: Mitos P-Pump Basic (Part No. 3200175)

    5 Mitos P-Pump Basic (Part No. 3200175) The Mitos P-Pump Basic connects to a PC via RS232 serial interface allowing data logging and control using free PC software or your own applications. A PC is required to use this pump. 5.1 Features Flow sensor module dock...

  • Page 18: The Following Accessories Are Supplied With The Mitos P-Pump Basic

    The following accessories are supplied with the Mitos P-Pump Basic: • Pneumatic supply cable • Tubing FEP 250 µ m ID, 1/16” OD, 2m length • Power supply unit • Mains IEC lead User to supply the following: • Suitable Windows ™ PC •...

  • Page 19: Power Connection

    The Mitos P-Pump Basic connects to a PC via RS232 serial interface allowing data logging and control using free PC software. For PCs that do not support an RS232 serial interface, Dolomite offer a USB adapter cable (Part No. 3200197). SHPT-487168127-329_v.2.0...

  • Page 20: Mitos Sensor Unit Electrical Connection

    Mitos P-Pump range via a fluidic connection for • use electrical cable supplied with the Mitos more information please see section 8. Sensor Unit For more information on Dolomite’s Mitos Sensor Units please visit our website www.dolomite-microfluidics.com/webshop SHPT-487168127-329_v.2.0 Page 20 of 60...

  • Page 21: Mitos P-Pump Remote (Part No. 3200176)

    (0 - 10bar) with excellent response time and accuracy. Pressure driven flow is ideal for microfluidic systems where highly stable flow is required in the nl/min to µl/min range for applications such as droplet formation. The design allows users to connect to Dolomite Remote Chambers with a push-click action. 6.1 Features Flow sensor...

  • Page 22: The Following Accessories Are Supplied With The Mitos P-Pump Remote

    The following accessories are supplied with the Mitos P-Pump Remote: • 2 x Pneumatic supply cable • Tubing FEP 250 µ m ID, 1/16” OD, 2m length • Power supply unit • Mains IEC lead User to supply the following: •...

  • Page 23: Power Connection

    6.4 Connection to Remote Chamber The Mitos P-Pump Remote features a pneumatic fitting which allows the connection to Dolomite’s Remote Chambers or custom chambers. As shown below, the Mitos P-Pump Remote is connected to the Mitos P-Pump Remote Chamber 30 (Part No. 3200178), a glass pressure chamber which connects securely to the pump via a pneumatic fitting.

  • Page 24: Mitos Sensor Unit Electrical Connection

    Mitos P-Pump range via a fluidic connection for • use electrical cable supplied with the Mitos more information please see section 8. Sensor Unit For more information on Dolomite’s Mitos Sensor Units please visit our website www.dolomite-microfluidics.com/webshop SHPT-487168127-329_v.2.0 Page 24 of 60...

  • Page 25: Mitos P-Pump Remote Basic (Part No. 3200177)

    For PCs that do not support an RS232 serial interface, Dolomite offers a USB adapter (Part No. 3200197). All the other features of the pump are the same as for the Mitos P-Pump Remote (Part No. 3200177) including pulseless pumping performance and easy to use pneumatic fitting.

  • Page 26: The Following Accessories Are Supplied With The Mitos P-Pump Remote Basic

    The following accessories are supplied with the Mitos P-Pump Remote Basic: • 2 x Pneumatic supply cable • Tubing FEP 250 µ m ID, 1/16” OD, 2m length • Power supply unit • Mains IEC lead User to supply the following: •...

  • Page 27: Power Connection

    The Mitos P-Pump Remote Basic connects to a PC via RS232 serial interface allowing data logging and control using free PC software. For PCs that do not support an RS232 serial interface, Dolomite offer a USB adapter cable (Part No. 3200197). SHPT-487168127-329_v.2.0...

  • Page 28: Connection To Remote Chamber

    7.5 Connection to Remote Chamber The Mitos P-Pump Remote Basic features a pneumatic fitting which allows the connection to Dolomite’s Remote Chambers or custom chambers. The Mitos P-Pump Remote Basic can connect to the Mitos P-Pump Remote Chamber 30 (Part No. 3200178), a glass pressure chamber which connects securely to the pump via a pneumatic fitting.

  • Page 29: Mitos Sensor Units Connection

    8 Mitos Sensor Units Connection The Mitos Sensor Display (Part No. 3200095) and Mitos Flow Rate Sensors (Part No. 3200096-3200100) are compatible with the Mitos P-Pump range for measuring and displaying ultra low liquid rates. The high precision thermal sensor technology provides total media isolation and extremely low internal volume with no moving parts.

  • Page 30: Connection To The Top Of The Mitos P-Pump

    8.2 Connection to the top of the Mitos P-Pump Connect tube from Mitos P-Pump to Flow Sensor Connect tube to system Fig. 2. Connection to top of a Mitos P-Pump Fig. 2. Connection to top of a Mitos P-Pump • •...

  • Page 31: Electrical Connection

    8.4 Electrical Connection To connect to Mitos Sensor Unit: • use electrical cable supplied with the Mitos Sensor Unit For more information on Dolomite’s Mitos Sensor Units please visit our website www.dolomite-microfluidics.com/webshop SHPT-487168127-329_v.2.0 Page 31 of 60...

  • Page 32: Controlling The Pumps

    The Mitos P-Pump Basic (Part No. 3200175) and Mitos P-Pump Remote Basic (Part No. 3200177) can only be controlled via the PC Software as described in section 11. Please contact our Technical Support Team support@dolomite-microfluidics.com receive a link to download the software online. The pressure pump above features The pressure pump above features the basic design;...

  • Page 33: Manual User Control

    10 Manual User Control This section only applies to Mitos P-Pump (Part No. 3200016) and Mitos P-Pump Remote (Part No. 3200176) Manual Control Functions P-Pump is starting. Please wait. P-Pump is initialising. Please wait. 10.1 Pressure Control Pressure control screen. N/A indicates that no target pressure has been selected.

  • Page 34: User Controls

    For example, for the supply pressure of 3500mbars it gives the range of 20mbars to 3250mbars. For negative supply pressure the target pressure can be set within the range of: MINIMUM PRESSURE: SUPPLY PRESSURE + 80mbars MAXIMUM PRESSURE: – 20mbars For example, for the supply pressure of -800mbars it gives the range of -720mbars to - 20mbars.

  • Page 35: Menu Option: Tare

    10.3 Menu Option: Tare The tare option opens the pressure chamber to atmosphere and resets the zero point for the pressure in the chamber. Once this has been set, then the Mitos P-Pump and Mitos P-Pump Remote will maintain an accurate zero point for long periods of time. The Mitos P-Pump and Mitos P-Pump Remote will automatically adjust to changes in atmospheric pressure, so it is not necessary to use the tare function frequently.

  • Page 36: Menu Option: Timer

    10.4 Menu Option: Timer The timer option enables the pressure to be controlled at a selected value for a set period of time before venting to atmosphere. The timer can be started after the Mitos P-Pump and Mitos P-Pump Remote is already controlling at a pressure to vent the chamber in a set period of time.

  • Page 37: Menu Option: Configuration - Mode

    10.6 Menu Option: Configuration – Mode The Mitos P-Pump and Mitos P-Pump Remote have two main modes of use. The first is static mode where the user selects a target pressure and the pump controls to that pressure. This is used in the majority of situations. The second is moving mode where the user dynamically controls the target using the twist-and-click control knob and the pump follows this moving target.

  • Page 38: Menu Option: Error Log

    10.8 Menu Option: Error Log To aid troubleshooting and provide information on issues with the Mitos P-Pump and Mitos P-Pump Remote, there is an error log. In the error log, all the previous messages for this session are recorded. The 3 categories of logged messages are information messages, warning messages and error messages.

  • Page 39: Menu Option: Usb Data Export

    10.9 Menu Option: USB Data Export The USB option allows pressure control data to be exported to a PC using a USB key. Each time the Mitos P-Pump and Mitos P-Pump Remote are turned on, a new data file is created.

  • Page 40: Menu Option: Screensaver

    10.10 Menu Option: Screensaver The screensaver option allows the user to enter the screensaver mode manually. In the main menu, select option 6 to access the screensaver mode. Screensaver mode. Scrolls between current pressure and target pressure. 10.11 Menu Option: Information This menu option provides information on the software version and the current atmospheric, chamber and supply pressure readings.

  • Page 41: Pc Software User Control

    (RS232) cable or USB to RS232 adapter cable. 1) USB to RS232 cable If the pump is connected using Dolomite’s USB to RS232 Adaptor Cable (Part No. 3200197) all devices will be detected automatically by the PC software and shown in the “P-Pumps”...

  • Page 42: Using The Pc Software

    11.2 Using the PC software The PC Software offers 3 sections as shown below: 1) Devices This section allows users to monitor and set the target and chamber pressure of the pump. It is divided into a “P-Pumps” section showing all pumps connected to the PC Software and “Flow Sensors”...
  • Page 43 Please note: Some of the above icons might be hidden due to the type of pump you are using and the pump state. When connecting the Mitos P-Pump or Mitos P-Pump Remote to the PC Software, the Connection Status will automatically change to “Being Controlled” and the pump display will show “PC CTL”...

  • Page 44: Using Flow Resistors

    This section displays all important events such as control commands sent to the pump/pumps, connection/disconnection of a device or device errors. 3) Help This section contains a link to Dolomite’s website and allows users to access and download the Mitos P-Pump datasheet. 12 Using Flow Resistors 12.1 Flow Resistors...
  • Page 45 1. If highly stable flow is critical to the application, it is also recommended that the connected system should be designed to have as low flow resistance as possible. Please contact Dolomite for assistance. The third case is where 2 or more fluids of similar viscosities are pumped into a junction and a high flow rate ratio is required (10:1 to 100:1).
  • Page 46 = 0-10 bar Flow resistor required – case 3 Dolomite also offers a wide range of Mitos P-Pump Flow Resistors (Part No. 3200028 – 3200032) to optimize your microfluidic system, from a minimum flow rate of 0.01 µl/min to a maximum flow rate of 1000µl/min . For more information please visit our website http://www.dolomite-microfluidics.com/webshop...

  • Page 47: Flow Rate Calibration

    12.2 Flow Rate Calibration If the flow resistor resistance is much higher than the resistance of the connected system, then the resistance of the connected system (R .µ ) can be ignored in determining the flow rate. Therefore, the flow rate from the pump is directly proportional to the pressure. As a result, the Mitos P-Pump range can be calibrated for a specific fluid with the flow resistor before connecting to the system.

  • Page 48: Accessories

    13 Accessories Dolomite offers a broad range of accessories including flow resistors, vessel holders and starter kits. Product Name Product picture Description Advantages Enabling the Mitos Mitos P-Pump P-Pump range to be used • Minimizes sample Vessel Holder Kit with a wide range of waste (Part No.
  • Page 49 For PCs that do not connect their Mitos USB to RS232 support an RS232 serial P-Pump Basic or Adapter Cable interface, Dolomite offers Mitos P-Pump (Part No. 3200197) a USB to RS232 Adapter Remote Basic to a PC Cable. SHPT-487168127-329_v.2.0...

  • Page 50: Accessories Compatibility

    13.1 Accessories compatibility Mitos Mitos P-Pump Mitos P-Pump Mitos P-Pump Accessories P-Pump Basic Remote Remote Basic Mitos P-Pump Vessel ✓ ✓ ✓* ✓* Holder Kit (Part No. 3200017) Flow Resistor F1, F3, F10, F30, F100 ✓ ✓ ✓* ✓* (Part No. 3200028 - 3200032) ✓* Mitos P-Pump Starter Kit...
  • Page 51 14 FAQ Why can’t I select a target pressure? Please check you have connected a compressed air / gas supply correctly (see section 4.4). Why can’t I select a target pressure as high as my supply pressure? To ensure quick response times, the maximum target pressure available is limited to 250 mbar below supply pressure.
  • Page 52 Either a flow sensor is required or the system can be calibrated for a selected fluid, so that a pressure can be translated into a flow rate. Please contact Dolomite for further advice. Why is there backflow into my Mitos P-Pump? When pumping 2 or more liquids using a Mitos P-Pump there is the possibility of backflow from one pump at higher pressure to another pump at lower pressure.
  • Page 53 How do I avoid gas dissolution in the liquid which is being pumped? Some liquids can absorb air when put under pressure and then release the air in the form of bubbles when the pressure is reduced. This occasionally causes problems with bubbles appearing after a flow resistor or a constriction.
  • Page 54 If you pumping 2 or more fluids into a junction and there is significant flow resistance downstream of the junction, then a flow resistor may be required (see section 10.1). Please contact Dolomite for further advice. 15 Power consumption specification The maximum power consumption for the P Pump range is 40 Watts (1.67 Amps at 24...
  • Page 55 The unit complies with all applicable essential requirements of the Directives. Signed by: ............... Name: Andrew Lovatt Position: CEO Done at The Dolomite Centre Ltd Unit 1, Anglian Business Park, Orchard Road, Royston, Herts, SG8 5TW, UK on 8 March 2010 SHPT-487168127-329_v.2.0...
  • Page 56 The unit complies with all applicable essential requirements of the Directives. Signed by: ............... Name: Andrew Lovatt Position: CEO Done at The Dolomite Centre Ltd Unit 1, Anglian Business Park, Orchard Road, Royston, Herts, SG8 5TW, UK on 8 September 2011 SHPT-487168127-329_v.2.0...
  • Page 57 The unit complies with all applicable essential requirements of the Directives. Signed by: ............... Name: Andrew Lovatt Position: CEO Done at The Dolomite Centre Ltd Unit 1, Anglian Business Park, Orchard Road, Royston, Herts, SG8 5TW, UK on 8 September 2011 SHPT-487168127-329_v.2.0...
  • Page 58 The unit complies with all applicable essential requirements of the Directives. Signed by: ............... Name: Andrew Lovatt Position: CEO Done at The Dolomite Centre Ltd Unit 1, Anglian Business Park, Orchard Road, Royston, Herts, SG8 5TW, UK on 8 September 2011 SHPT-487168127-329_v.2.0...
  • Page 59 [Page intentionally left blank] SHPT-487168127-329_v.2.0 Page 59 of 60...
  • Page 60 Dolomite Microfluidics 3 Anglian Business Park, Royston, SG8 5TW, UK T: +44 (0)1763 242491 E: info@dolomite-microfluidics.com W: dolomite-microfluidics.com SHPT-487168127-329_v.2.0 Page 60 of 60...

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