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Related Manuals for Raptor Photonics Owl 640 II Series
Summary of Contents for Raptor Photonics Owl 640 II Series
- Page 1 Owl 640 II Model: OW1.7-VS-CL-640-II USER MANUAL OWL 640 II/USER MANUAL/08-20/REV1.1...
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Page 2: Table Of Contents
CONTENTS 1. INTRODUCTION ......................4 1.1 Scope ......................... 4 2. CAMERA CARE ....................... 5 2.1 Cleaning the Sensor Window ................... 5 3. SPECIFICATION ......................6 3.1 Camera Overview ...................... 6 3.2 Datasheet ........................6 4. DESIGN OVERVIEW ......................7 4.1 Mechanical Model ...................... 7 4.2 Physical Interface ...................... - Page 3 8.5 Thermoelectric Cooler (TEC) .................. 21 8.6 Miscellaneous Tab ....................22 8.7 Manufacturers Data ....................23 9. XCAP CONTROL FEATURES ..................24 9.1 Recording Images on XCAP ................... 24 9.2 Saving Preset Configuration Settings ..............24 9.3 Contrast Modification (XCAP Std. Only) ..............25 10.
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Page 4: Introduction
1. INTRODUCTION This document provides detailed instructions for the operation of the Owl 640 II camera. Raptor Photonics Limited reserves the right to change this document at any time without notice and disclaims liability for editorial, pictorial or typographical errors. -
Page 5: Camera Care
2. CAMERA CARE 2.1 Cleaning the Sensor Window Raptor cameras require no regular maintenance except occasional external cleaning of the sensor window (the glass window between the camera sensor and the microscope or lens). Use optical grade isopropyl to clean this window. A cotton swab can be used, but may leave some fibres on the window, so be careful. -
Page 6: Specification
Camera Link standard frame grabber. 3.2 Datasheet For the full specification of the Owl 640 II, the datasheet for the camera can be downloaded from the Raptor Photonics website using the link below: https://www.raptorphotonics.com/products/owl-640-ii/ OWL 640 II/USER MANUAL/08-20/REV1.1... -
Page 7: Design Overview
4. DESIGN OVERVIEW 4.1 Mechanical Model Units shown in mm and [inches] [in] PDF of mechanical model available from our website: https://www.raptorphotonics.com/products/owl-640-ii/ OWL 640 II/USER MANUAL/08-20/REV1.1... -
Page 8: Physical Interface
4.2 Physical Interface 1. SMA connector: Trigger Out 2. SMA connector: Trigger In 3. Power 4. CameraLink Connector/SDR 4.3 Power Consumption Unit input power specification is +12V DC +/- 0.5V with <3W power dissipation when the TEC is disabled. An additional 5W may be required if the full drive to the TEC is required. This will be dependent on environmental conditions and the TEC temperature set point. -
Page 9: Software Compatibility
5. SOFTWARE COMPATIBILITY This section outlines the options relating to software that are available for the Owl 640 II. 5.1 XCAP Compatibility Raptor works closely with EPIX who integrate all Raptor camera models into their XCAP Imaging Software package. XCAP is the core plug and play software package that is offered with the Owl 640 II. -
Page 10: Camera Setup And Requirments
6. CAMERA SETUP AND REQUIRMENTS This section will give instruction on connecting the frame grabber to the camera, as well as outlining important frame grabber and PC requirements. 6.1 Connecting the Camera to the Frame Grabber The camera has the shrunk SDR port on the interface. The main frame grabber that Raptor offer with this camera has the normal MDR port (EPIX EB1). -
Page 11: Xcap Imaging Software
7. XCAP IMAGING SOFTWARE This section will discuss downloading and acquiring an image using XCAP. 7.1 Downloading XCAP The latest version of XCAP can be downloaded from the link below: http://www.epixinc.com/support/files.php Please select the appropriate version of XCAP for your computer. Ensure that you download from the section labelled “Pre-release version with support for the latest cameras and latest PIXCI®... - Page 12 Using the dropdown menu highlighted, search for “Owl 640 II”. You will see the configuration for “Raptor Photonics Owl 640 II”. Selecting “Open w. Default Video Setup” will open the control panel with all control parameters set to the default states. “Open w. Last used Video Setup”...
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Page 13: Acquiring A Live Image Sequence
7.3 Acquiring a Live Image Sequence There are two things to observe in the control panel that inform you that the camera is connected and ready to image. The serial connect checkbox must be ticked in the control panel. This informs you that you have established a serial connection with the camera and can control the camera. -
Page 14: Controlling The Camera (Xcap)
8. CONTROLLING THE CAMERA (XCAP) The sections below will give information on using each control of the Owl 640 II, giving a description on how to use each control parameter and their effect on the camera’s performance. The software used to illustrate the camera controls is XCAP. 8.1 Exposure Time The exposure time can be controlled under any camera control tab in the XCAP GUI and remains constant on the GUI. - Page 15 The exposure time controls on XCAP are shown in Figure 7. Figure 7: ALC and Manual Exposure Time Controls. OWL 640 II/USER MANUAL/08-20/REV1.1...
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Page 16: Automatic Light Control Adjustment
8.2 Automatic Light Control Adjustment The ALC can be fine-tuned by adjusting a few different parameters. A Region of Interest (ROI) of pixels can also be defined in which to drive the ALC. These controls are discussed below. 8.2.1 Automatic Light Control Parameters Peak and average video levels are derived and monitored for the active ROI and are used to drive the ALC. -
Page 17: Automatic Light Control Region Of Interest
8.2.2 Automatic Light Control Region of Interest A Region of Interest (ROI) within the main active region of 640 x 512 may be defined. This region is used to calculate peak and average video levels to drive the Automatic Light Control (ALC) function of the camera (discussed in section 8.3.1). -
Page 18: Gain Mode
8.2 Gain Mode The camera has two modes of operation, high gain mode and low gain mode (default). The gain mode of the camera can be toggled from the “Gain” tab on the GUI. If the ALC is enabled, the gain mode will be controlled automatically depending on the imaging scene illumination. -
Page 19: Trigger Mode And Frame Rate
8.3 Trigger Mode and Frame Rate Trigger Mode: The trigger mode of the camera and additional trigger controls can be controlled from the “Trigger” tab in the GUI, shown in Figure 11. The trigger mode of the camera can be toggled from the “Readout Mode” dropdown box. By default, the camera will be set to internal trigger, integrate then read (ITR). -
Page 20: Non-Uniformity Correction (Nuc)
8.4 Non-Uniformity Correction (NUC) The NUC state of the camera can be controlled from the “NUC” tab of the GUI. The camera has a 3 point (offset, gain, dark) NUC performed live on board the FPGA of the camera, correcting for fixed pattern noise. There is also a bad pixel correction when the 3 point NUC is active. -
Page 21: Thermoelectric Cooler (Tec)
8.5 Thermoelectric Cooler (TEC) The Owl 640 II uses a TEC to stabilise the sensor temperature at 15 ͦ C . The TEC status is shown in the “TEC” tab of the XCAP GUI, shown in Figure 13. The TEC control can be enabled/disabled from this tab. By default, the TEC will be enabled and set to a 15 ͦ... -
Page 22: Miscellaneous Tab
8.6 Miscellaneous Tab The miscellaneous tab displays some minor controls for the camera, explained below: Video Invert: Inverts the video pixel values Active Image Enhancement: Sharpens Image Bad Pixel: Selecting “Highlight” will show the bad pixel map on the image that the NUC is correcting for. -
Page 23: Manufacturers Data
8.7 Manufacturers Data The “Info” tab displays the manufacturers data of the camera, such as the firmware version and serial number etc. The PCB and sensor temperature can be read back from this tab by clicking “Update Temp.” The “Info” tab is shown in Figure 15. Figure 15: Manufacturers Data. -
Page 24: Xcap Control Features
9. XCAP CONTROL FEATURES XCAP has many different control functions and analytical tools that can be used when imaging the camera. For the full XCAP user guide, please refer to the link below: http://epixinc.com/manuals/pixci_e14el/index.htm This section will discuss in detail a few features on XCAP that Raptor thinks would be of immediate use when using the camera. -
Page 25: Contrast Modification (Xcap Std. Only)
9.3 Contrast Modification (XCAP Std. Only) The image contrast can be modified from the “Contrast Modification” section under the “Modify” tab in the XCAP imaging window. The location of this control feature is shown in Figure 17. Figure 17: Contrast Modification Location on Toolbar. In the contrast modification box, that can be seen from Figure 18, select “Stretch Contrast, Histogram Percentile Endpoints”... -
Page 26: Micro-Manager
10. MICRO-MANAGER Micro-Manager is a free open source software package that enables you to image and control the Owl 640 II. Primarily, it is a software package designed for microscopy applications, as the software provides control of microscopes as well as the camera. However, Micro-Manager can also be used to simply image the Owl 640 II and control each of its parameters. -
Page 27: Creating Camera Configuration File
10.2 Creating Camera Configuration File After loading up Micro-Manager, the start-up configuration window will pop up as seen from Figure 19. The user should ensure that “(none)” or “MMConfig_demo.cfg” is selected from the dropdown list and click “Ok”. Figure 19: Start-up Configuration – Demo. OWL 640 II/USER MANUAL/08-20/REV1.1... - Page 28 The main Micro-Manager window will now appear. To access the hardware configuration wizard to create the configuration file, go to the tools tab on the toolbar, shown in Figure 20. Figure 20: Hardware Configuration Wizard Location. Figures 21 – 25 outlines the process to create an Owl 640 II (.cfg) file. After selecting the Hardware Configuration Wizard from the main toolbar, the wizard will pop up.
- Page 29 Figure 22: Available Devices. After selecting the Owl 640 (Owl 640 & Owl 640 II use the same option), a pop up window will appear with the device and property values stated in Figure 23. Click “Ok”. Figure 23: Device and Property Values. Under “Installed Devices”, the Owl 640 II will now be listed (Figure 24).
- Page 30 Figure 24: Installed Devices. The user should now click “Next” multiple times until reaching the window shown in Figure 25. Once at this stage, the final step is to choose the name of the configuration file and where to store it. The common place is to store in the Micro-Manager-1.4 folder, usually stored in “C:\Program Files\Micro-Manager”.
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Page 31: Pre-Made Configuration File
After finishing the steps outlined above, the user will now be redirected back to the main Micro-Manager window where you can now image and control the camera. 10.3 Pre-made Configuration File Raptor can supply a premade configuration file that has already been created, with all important controls extracted from the device property browser (section 10.4) to the main Micro-Manager window. -
Page 32: Imaging And Controlling The Camera
10.4 Imaging and Controlling the Camera The full Micro-Manager User Guide can be obtained from the link below: https://micro-manager.org/wiki/Micro-Manager_User%27s_Guide This user guide gives a full breakdown on using all features and analytical tools of Micro- Manager. To access the controls of the camera, select “Device Property Browser” from the “Tools” tab on the toolbar of the main Micro-Manager window, shown in Figure 27. - Page 33 Figure 28: Device Property Browser. To understand each control parameter of the Owl 640 II, please refer to section 8 that gives detailed and important information of using each camera control and the effect that it has on the camera performance. Please Note that the controls in Micro-Manager are listed in alphabetical order in the device property browser.
- Page 34 CORPORATE HEADQUARTERS Raptor Photonics LTD Willowbank Business Park Larne, Co Antrim BT40 2SF Northern Ireland PH: +44 2828 270141 www.raptorphotonics.com OWL 640 II/USER MANUAL/08-20/REV1.1...
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