Optical Setup ......................12 Settings/Additional Information ................. 13 Routine Care and Maintenance .................. 14 Fitting the pE-100 to a Different Microscope ............. 15 pE-100 Liquid Light Guide Option ................16 Product Specifications ....................18 Product Options and Order Codes................18 Warranty and Repairs ....................
Introduction CoolLED’s pE-100 has been developed for applications requiring a single LED excitation wavelength. It is perfect for clinical applications such as routine screening (e.g. using Auramine for Tuberculosis), for research applications requiring precise intensity control and fast-switching, or for electrophysiology applications where light has to be delivered to a specific location.
Safety Precautions While LEDs are a much safer illumination system than the mercury and metal halide lamps that they replace in microscopy applications, precautions should still be taken with this product. When operating or maintaining this product, please observe the following safety precautions at all times.
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2.6. Any electronic equipment connected to this product must comply with the requirements of EN/IEC 60950. 2.7. To clean the exterior of the Light Source, use a slightly dampened cloth with a simple water/detergent solution only. Avoid the optical surfaces and lenses.
Getting Started – System Components The CoolLED pE-100 Illumination System is supplied with the following components: 1. LED Light Source. 2. Manual Control Pod. 3. Microscope adaptor for specific microscope model (Direct attachment version only). 4. Liquid light guide (light guide version only).
Connect the power connector from the DC power supply to the Control Pod as shown. Ensure that the DC power supply is the one supplied with the product. Using non CoolLED power supplies may damage the Light Source and will invalidate the warranty. At this stage do not connect the mains power lead to the DC power supply.
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4.4. Attach the LED Light Source to the epi-fluorescence port on your microscope. Your pE-100 will have been supplied with a compatible fitting for the microscope you specified at order. Attach the Light Source ensuring that it is secure and true/flush with the microscope.
Operation - Manual Control 5.1. Manual Control Pod Operation on/off. The pE-100 is easily controlled from the manual Control Pod. LEDs are switched on and off by pressing the ‘on/off’ button. State of light source displayed here. Either OFF or ON.
Remote Operation – TTL 6.1. The pE-100 can be controlled remotely via a TTL signal. 6.2. TTL control uses the single BNC socket on the reverse of the Control Pod. 6.3. The TTL signal controls the on/off function of the Light Source. A TTL ‘high’...
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6.4. The TTL input circuit has been designed to maximise the switching speed of the LEDs to give the user precise control of the excitation light reaching the sample. Note: Switching speeds vary slightly depending on LED wavelength and the intensity that is used. 6.5.
Optical Setup 7.1. The pE-100 has been designed to work on the majority of fluorescence microscopes, both new and old. As would be expected, there is some variation in the optical path and elements within every microscope. In order to accommodate these variations, the pE-100 is supplied with a small adjustment which allows the user to optimise the performance of the illumination system when it is first fitted.
Settings/Additional Information 8.1. Display Backlight and Contrast settings. Upon switching on the Control Pod a chequered pattern shall appear on the display. If any button is pressed at this stage, then you shall be taken through a setup sequence. The first option displayed is the contrast adjustment.
Routine Care and Maintenance 9.1. The pE-100 requires little or no maintenance throughout its life. There are no field serviceable parts so there is no need to remove the covers. 9.2. Cleaning of the external surfaces can be carried out with a mild soap and water solution used to lightly dampen a lint-free cloth.
10.2. If you intend to use the pE-100 on a different microscope than the make and/or model that it was originally intended for, please contact CoolLED to check whether the Light Source will be compatible in its current form. We will be able to advise whether an adaptor swap or optical adjustment is required.
Liquid Light Guide Option 11.1. The pE-100 is available with a 3 mm liquid light guide rather than using a direct attachment adaptor. This enables the Illumination System to be used on microscopes which just have a liquid light guide input, the collimating optics being an integral part of the microscope.
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11.4. The use of a liquid light guide will be attractive for use in electrophysiology as this allows the Light Source to be placed outside the Faraday cage to reduce electrical noise close up to the samples. The pE-Universal Collimator is available for these applications.
- weight 0.19 kg 12.4. Environmental Operating Conditions Operating 5 – 35 °C Product Options and Order Codes See website (Microscope Illuminators | LED Illumination Systems | CoolLED) for full details of product options and order codes. DOC-012 Iss 7...
End-of-Life Light Sources. You can help us by filling in our online contact form and providing us with your contact details and the serial number of the CoolLED Light Source that you wish to return and we will collect it free of charge.
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