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Related Manuals for Okolab H301-EC-LG-BL
Summary of Contents for Okolab H301-EC-LG-BL
- Page 1 User Manual H301-EC-LG-BL REV 03 H301-EC-LG-BL Compatible with the following Z-Piezo stages Compatible with the following Okolab Controllers • • LEICA super Z Galvo stage (SP5/SP8) H301-T-UNIT-BL-PLUS • UNO-COMBINED-CONTROLLER • H401-T-DUAL www.oko-lab.com...
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Page 2: Table Of Contents
User Manual H301-EC-LG-BL REV 03 Index Components and dimensions....................3 Sample Holders ........................4 2.1 Available Sample Holders ....................... 4 Insertion of the Sample Feedback Temperature Sensor ..............5 Insertion of Sample Holder into Chamber ................... 5 Working with 35 or 60 mm Petri Dish ..................5 Working with 1x3’’... -
Page 3: Components And Dimensions
User Manual H301-EC-LG-BL REV 03 1. Components and dimensions H301-EC-LG-BL includes the following components: • Chamber main body with embedded temperature sensor. • Heated glass lid with Indium Tin Oxide (ITO) conductive coating and embedded temperature sensor. • Total chamber weight: about 130 g. For details, see the table at page 4. -
Page 4: Sample Holders
For #1 standard chamber slide For #1 35mm Petri-dish holder holder Figure 2.Available sample holders. H301-EC-LG-BL has a different weight in relation to sample holder used. The following table shows the weight of all possible configurations. H301-LG-BL+SAMPLE HOLDER WEIGHT (g) …………………….H301-EC-LG-1x35... -
Page 5: Insertion Of The Sample Feedback Temperature Sensor
3. Insertion of the Sample Feedback Temperature Sensor Insert the Sample Feedback Temperature Sensor through the dedicated opening, labeled ‘T Sensor’, located on the H301-EC-LG-BL base (see Figure 3, Frontal and 3D views). Temperature Sensor dedicated opening Temperature Sensor dedicated opening 1.Frontal view... - Page 6 User Manual H301-EC-LG-BL REV 03 Figure 5. Plate adapter for 35mm Petri-dish. Figure 6. Plate adapter for 60 mm Petri-dish. www.oko-lab.com...
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Page 7: Working With 1X3'' And 1X2'' Chamber Slides
User Manual H301-EC-LG-BL REV 03 6. Working with 1x3’’ and 1x2’’ chamber slides Flat springs prevent movement of 1’’x 3’’ and 1’’x 2’’ chamber slides inside of the sample holder. Figure 7. Plate adapter for Lab-TeK 1”x2” chambered cover glass. -
Page 8: Connecting The Gas Supply
Figure 9.Connection with gas supply. 8. Working with Perfusion The H301-EC-LG-BL features 4 perfusion holes on the side for the insertion of perfusion tubing up to 2.0 mm in outer diameter. Small screws plug the perfusion holes when not in use. -
Page 9: Connecting The Chamber With Super Z-Galvo Stage
9. Connecting the Chamber with Super Z-Galvo stage H301-EC-LG-BL fits on the Super Z-Galvo stage. It can be screwed on the Galvo Stage as shown in Figure 11; place the chamber on the stage and screw the four slotted head pan screws. Screws housings are indicated with letter A in Fig- ure 11. -
Page 10: Cleaning
User Manual H301-EC-LG-BL REV 03 10. Cleaning • Turn the system off and pull the mains plug out the socket • Remove the lid from the chamber main body, and keep it separate from the chamber main body while the chamber cools down.