Preface CO2-O2-UNIT-BL [0-20; 1-95] is a Digital CO2/O2 Controller that mixes CO2, O2 and N2 to the desired concentration in the CO2 range 0-20% and O2 range 1-95%. If operated without N2, it mixes CO2 and Air to the desired CO2 concentration in the range 0-20% and you don’t need to regulate O2.
Symbol description This paragraph describes the symbols used in this manual and on the product label. Symbols used in this manual The following symbols identify important information to note: CAUTION or WARNING: this symbol warns you about the risk of electrical shock. CAUTION or WARNING or IMPORTANT: this symbol warns you of circumstances or practices that can affect the functionality of the instrument.
Safety Notes In order to achieve maximum performance and to ensure proper operation of your new equipment, please read carefully the following safety notes and the instructions. If you have any question, please contact Okolab. − The equipment must only be used as intended and as described in this Manual.
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We reserve the right to make technical variations. IN NO EVENT SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL DAMAGES OF ANY NATURE, OR LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE...
) on the rear panel of the CO2-O2-UNIT-BL [0-20; 1-95]. 5. Pressure Gauge for CO2 + regulator + assembly stirrup (x1). Install pressure gauge between the pure CO2 supply pressure regulator and the corresponding CO2 input port on the rear panel of CO2-O2-UNIT-BL [0-20; 1-95].
Figure 1. CO2-O2-UNIT-BL [0-20; 1-95] – components. GAS supplied requirements − CO2. CO2 must be available at 1.4 barg (20.3 psig), with a connector for 6mm OD rigid silicon tube. Gas source must be Standard Purity CO2 (coded as 4.5 that means 99.995 % of CO2) and humidity-free gas.
Compatibility CO2-O2-UNIT-BL [0-20; 1-95] is compatible with: • OKO-TOUCH (required), including: − Touch Screen Interface − #1 RS232 Serial cable. To connect the Touch Screen to the unit. − MINI-USB-OTG cable. To connect a USB pen to the OKO-TOUCH for data download.
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• DATA-LOG. Software used to log, view, and store data from a PC. It may be used to change parameters of the controllers via PC. Data can be exported in .xlsx format. Figure 3. DATA LOG software. • SM-BL Smart Box. To store data, to operate the controllers via web/smartphone/tablet and to allow remote assistance for service.
3. Gas Output. Mixed gas input: valved in-line connector. 4. Power Input. 5. OKO-AIR-PUMP-BL connector. Leave unplugged if CO2-O2-UNIT-BL [0-20; 1-95] is not equipped with OKO- AIR-PUMP-BL. 6. HM-ACTIVE connector. Leave unplugged if CO2-O2-UNIT-BL [0-20; 1-95] is not equipped with HM-ACTIVE.
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Figure 5. Gas Controller Rear Panel Overview.
1. Install the pressure Gauge for the CO2 between the CO2 supply and CO2-O2-UNIT-BL [0-20; 1-95]. Follow the arrow on the rear of the Pressure Gauge for the correct Gas In-Out (see Figure 6).
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Figure 8. Pressure gauge using. 2. Install the TUBE-CB on the rear panel of CO2-O2-UNIT-BL [0-20; 1-95]. It filters the mixed gas before it enters in contact with CO2-O2-UNIT-BL [0-20; 1-95] gas sensors. Connect the TUBE-CB with the Filter In and Filter Out connectors on the rear panel of CO2-O2-UNIT-BL [0-20;...
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1-95] (9 in Figure 5) by using TUBE-AF. Push the valved in-line connector of TUBE-AF into O2 Input on the rear panel of CO2-O2-UNIT-BL [0-20; 1-95] until you hear a lockout click (see Figure 11 a). Set O2 pressure within the range 0.6 – 1 barg (8.7 – 14.5 psig).
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5. Figure 12 shows the connections between the CO and O tanks and the respective connectors on the rear panel of CO2-O2-UNIT-BL [0-20; 1-95] when you work in CO2/O2/N2 mode. TUBE AF TUBE A NOT supplied...
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6. Figure 13 shows the connections between the CO2 and Air and the respective connectors on the rear panel of CO2-O2-UNIT-BL [0-20; 1-95] when you work in CO2 in Air mode. In this case, you have to connect the Air supply to the N2 connector on the rear panel of CO2-O2-UNIT-BL [0-20;...
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7. Connect the valved in-line connector of the TUBE-AL to the gas port labeled “Gas output” on the rear panel of CO2-O2-UNIT-BL [0-20; 1-95] and the other side of the TUBE-AI to your device, as shown in Figure 15. Gas Input...
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9. Connect the power adapter to CO2-O2-UNIT-BL [0-20; 1-95] (7 in Figure 1). 10. To switch CO2-O2-UNIT-BL [0-20; 1-95] on use the power button on OKO-TOUCH. The Status LED (1 in Figure 4) on the front of CO2-O2-UNIT-BL [0-20; 1-95] will turn green.
Figure 18. CO2-O2-UNIT-BL [0-20; 1-95] and H301-T-UNIT-BL-PLUS. CO2-O2-UNIT-BL [0-20; 1-95] must stack on top of the Bold Line T controller by lining up the bus ports located on the top and bottom surface of each unit. When the units are properly connected the Temperature and parameters will appear on the OKO-TOUCH Touch Screen interface Home page.
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Figure 19. CO2-O2-UNIT-BL [0-20; 1-95] and H301-T-UNIT-BL PLUS on stock. If you have CO2-O2-UNIT-BL [0-20; 1-95] and a Bold Line T controller, it is essential to read both this manual and the Bold Line T controller User Manual to familiarize yourself with the...
8. Settings. Press here to access system options and settings. 9. Home. To open the homepage. 10. Product info. Press here to know generic info about CO2-O2-UNIT-BL [0-20; 1-95] and running time. Tip ►The OKO-TOUCH is pre-set at 5% CO and 5% O 9.1.1...
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Cancel to undo. Tip ►After any change in the Setpoint value, CO2-O2-UNIT-BL [0-20; 1-95] enters into a transient regime, the Status Indicator and the TOP LED (see Figure 23) turn yellow (see paragraph 9.1.2). During the transient...
The YELLOW color indicates that the controller is in transient regime. The Yellow light will appear after the controller is turned on and after any Setpoint change. The system is working properly, it is not in alarm and no action is needed. As soon as CO2-O2-UNIT-BL [0-20; 1-95] reaches the CO and/or O composition, CO2-O2-UNIT-BL [0-20;...
C02-O2-UNIT-BL [0-20; 1-95] can work in two alternative modes: • O2/N2/CO2 mode. In this control mode CO2-O2-UNIT-BL [0-20; 1-95] is supplied with Nitrogen, Oxygen and CO2 from respective tanks and you can regulate CO2 in the range 0-20% and Oxygen in the range 1- 95%;...
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You can switch from the standard O2/N2/CO2 mode to the CO2 in Air mode and back by the Touchscreen, as described below. Press the icon to set which Control Mode you want to use (see Figure 26 a). Note ► The O2/N2/CO2 mode is already set by default. Figure 26.
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In the case you are using OKO-AIR-PUMP-BL please note that it can be turned on/off only via the Touch Screen interface (OKO-TOUCH). Thus, it is essential that you hereby set the correct Air source. Press on Air Pump button (see Figure 27 a) to change the Air pump speed. You can add an offset in the range [-10;...
Figure 30. Homepage operated in CO2 in Air mode. 9.2.1.2 Flowrates Press the Flowrates icon to check how many liters per minute of O , CO and N the system is consuming (see Figure 31 a and b). Additionally, this panel allows you to select the flowrate of the gas mixture you want to send to your incubator/device.
Figure 33. C02-O2-UNIT-BL [0-20; 1-95] flowrate for values below 0.2 l/min. Note ► You can set the flowrate at 0.0 l/min, in this case the gas flowrate will be paused and output flow will be interrupted (see Figure 33). In this working mode three dots will appear in Homepage instead of CO and O % current value and the indicator will turn yellow.
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1. Choose the two CO and O concentrations between which you want to switch (SP1: Setpoint 1 and SP2: Setpoint 2, as shown in Figure 36). 2. Set the duration in hours and minutes you want the system to dwell on each concentration (1 in Figure 37). 3.
Figure 41. You can press Abort to abort the cycle. Pressing “View” will open the current cycle settings. Press Home to undo. Figure 41. Warning shown if you try to change Setpoint during a gas cycle. 9.2.1.4 Status Press the Status icon (see Figure 42 a) to check CO2-O2-UNIT-BL [0-20; 1-95] performance.
Figure 42. Status page (a – b). Note ► The Status page contains advanced technical data. You may be asked to read these data in case of technical assistance with Okolab engineers. 9.2.1.5 Offset (with exthernal meter) Press on Offset icon to calibrate the instrument by LEO as external meter (NOT provided).
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4. Start a Gas Single Point measurement in Diffusion Mode on LEO (please refer to LEO User Manual). LEO (not supplied) CO2-O2-UNIT-BL [0-20; 1-95] TUBE-AG TUBE-A (supplied with LEO) Figure 45. External Gas Meter Connection. 5. Wait until LEO stabilizes the CO2 and O2 readings; this may take 10-15 minutes.
Figure 47. Manual Calibration Correction. Note ► By pressing Adj CO2 / Adj O2, the system will perform a calibration using the External Probe as a reference. The offset resulting from the calibration will be displayed in the Offset tab (see Figure 48). Figure 48.
Press the Digital Input icon to access to Digital Input page (see Figure 50 a). Press the CO2 Digital Input icon (see Figure 50 b) and / or O2 Digital Input icon to activate the Digital Input on CO2 and /or O2 Setpoint. Figure 50.
9.2.2.1 Touch Screen Options Press the Options icon (see Figure 53 a) to enter the Touch Screen Options page. The Display Options menu allows to set the time frame in which the minimum and maximum CO values are collected. To insert the time frame, press the + / - icons or scroll the Chart history length bar (1 in Figure 53 b). Note ►...
Never, the Top LED will never illuminate, if you select On Alarm, the TOP LED will illuminate only when CO2-O2-UNIT-BL [0-20; 1-95] is in Alarm Status (see paragraph 9.1.2), if you select Always, the TOP LED will always illuminate.
9.2.2.5 Alarms CO2-O2-UNIT-BL [0-20; 1-95] also allows activating visual and audible alarms. To set the alarms specifications, press the Alarms icon (see Figure 57 a), then follow the indications below: 1. Click on the Gas icon to set the gas deviation for a period of time then the system triggers on Alarms, as shown in the Figure 57 b.
9.2.2.6 Data Logging CO2-O2-UNIT-BL [0-20; 1-95] touch screen interface, OKO-TOUCH (see Figure 2), is equipped with on- board memory for data logging and storage. A simple routine allows downloading data to USB drive or to PC. In order to log and then download the logged data, connect a USB drive (not supplied) to OKO-TOUCH, using MINI-USB-OTG cable (provided), as shown in Figure 59.
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Tip ► When activating the logging on the internal memory, you can access the Logging page also by pressing the activated logging icon on the Homepage, as shown in Figure 62 Figure 61. Logging. (a) How to enter in the logging menu; (b) logging in internal memory. Figure 62.
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5. Write the file name suffix to be reported in the downloaded files by pressing on the keyboard icon , see Figure 64 a. Press Save, see Figure 64 b. Tip ►With the selections as in Figure 63 and in Figure 64, the data will be downloaded on the USB drive as shown in Figure 65.
Figure 67. Download to USB. You can activate the logging also on the USB drive, by flagging on USB drive, see Figure 68 a. Figure 68. Logging on USB flash drive. (a) USB drive selection; (b) Reminder to connect the USB drive. In this case, OKO-TOUCH warns you that a USB drive must be connected to the USB port, see Figure 68 b, and will store the data on the USB drive.
Press the icon to access the Info Page, as shown in Figure 72. This page contains the information related to the OKO-TOUCH (as shown in Figure 73 a) and to CO2-O2-UNIT-BL [0-20; 1-95] version (as shown in Figure 73 b).
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Figure 72. Info page selection. Figure 73. Info page OKO-Touch (a) and CO2-O2-UNIT-BL [0-20; 1-95] (b). Tip ►Please have this information handy when contacting Okolab for support...
10 Touch Screen Calibration Keep pressed the ON/OFF button on OKO TOUCH for 7 seconds to start the Touch Screen Calibration (see Figure 74). While holding the button, the pop-up message shown in Figure 75 a appears. Then tap blue calibration dots until the calibration is complete (see Figure 75 b).
Liquids should not be entered inside CO2-O2-UNIT-BL [0-20; 1-95]. Maintenance − Verify periodically the status of all hoses/tubing. If some hoses/tubing is damaged, contact Okolab to receive assistance. − After 2 years, disconnect all polyurethane tubing, cut the last 1 cm of the tubing and reconnect them.
To contact one of our engineers please write to support@oko-lab.com or contact us through the live chat in www.oko-lab.com. You can request a remote support session anytime. Please, do not hesitate to contact Okolab should you need any further commercial information or technical support. HARDWARE SUPPORT: sibillo@oko-lab.com Phone...
14 Troubleshooting We have collected in the table below some frequently asked questions, please contact Okolab if you need support. Symptom Probable cause Remedy No power supplied to Unit Power plug is disconnected Connect plug securely Check gas tanks and/or compressed lines...
CO2-O2-UNIT-BL [0-20; 1-95]............. 18 Figure 13. CO and Air tanks connections to the back panel of CO2-O2-UNIT-BL [0-20; 1-95]..............18 Figure 14. CO and Air Pump connections to the back panel of CO2-O2-UNIT-BL [0-20; 1-95]..............19 Figure 15.
Figure 71. Display modalities. (a) Icon Mode; (b) Glance Mode..........................44 Figure 72. Info page selection........................................45 Figure 73. Info page OKO-Touch (a) and CO2-O2-UNIT-BL [0-20; 1-95] (b)......................45 Figure 74. How to enter in Touch Screen Calibration..............................46 Figure 75.
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LIMITATION OF LIABILITY: the total liability of Okolab S.r.l. shall not exceed the purchase price of the component upon which liability is based. In NO event shall Okolab S.r.l. be liable for consequential, incidental or special damage.
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