TABLE OF CONTENTS Foreword Safety precautions and application limits Pipette handling Maintenance and sterilisation Charging stand, power handle and power supply Instrument description View Keys and functions Start button LCD display Power handle Optional charging stands Power supply Use of the instrument Supply contents Inserting power handle Replacing power handle...
Foreword Congratulation for choosing a Socorex instrument. You purchased a superior quality product built to last, and to satisfy you for a long time. The Acura ® electro pipette allows precision liquid handling without hand fatigue. The microproces- sor-controlled instrument includes an extended life NiMH battery pack for high performance.
SAFETY PRECAUTIONS AND APPLICATION LIMITS Before using the instrument for the first time, read the safety precautions and application limits carefully. Socorex will not assume any responsibility for problems related to erroneous use of the instrument. Pipette handling Refer to and follow regulations about handling...
INSTRUMENT DESCRIPTION ® The Acura electro is a motorized, microprocessor controlled air displacement pipette. Energy is provided by a long life NiMH battery pack located in the pipette handle. This power handle can be replaced with a fully charged one within seconds, thus ensuring continuous work-flow without loss ®...
USE OF THE INSTRUMENT Supply contents ® Accessories supplied with the Acura electro may vary, depending on country. Check exact contents on packaging label. All elements also available separately. Refer to chapter “ Ordering information ” for more details. Note : Keep original packaging for adequate instrument protection during future transport or shipment. Inserting power handle (fig.
PARAMETER SETTING Holding the electronic pipette (fig. 1) ® The ergonomic shape of the Acura electro allows long pipetting series without hand fatigue. Place finger rest on the phalanx of forefinger. The thumb reaches naturally start button (1) and ejector button (7), both easily activated. Multichannel casing revolves to allow the selection of the best working position.
PARAMETER SETTING Forward mode, programming and pipetting In forward mode, the exact volume desired is aspirated. Liquid dispensing is automatically fol- lowed by a short excess plunger movement (blow out). Plunger returns back to its initial position one second later. OPERATION ACTION KEY / BUTTON...
PARAMETER SETTING Reverse mode, programming and pipetting In reverse mode, the liquid is aspirated in excess of the selected volume. However, the set volume only will be delivered. The excess volume remains in the tip and can be kept or discarded. The reverse pipetting mode is recommended for viscous, volatile or foaming liquids.
PARAMETER SETTING Stepper mode, programming and pipetting In stepper mode, the volume aspirated is in small excess of the sum of every single aliquot. It is distributed step by step according to volume and number of aliquots programmed by the user. OPERATION ACTION KEY / BUTTON...
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PARAMETER SETTING Pipetting in stepper mode Press start button gently until first stop to work at the slow pipetting speed. Press fully (second stop) to work at selected pipetting speed (fig. 2A). OPERATION ACTION KEY / BUTTON LCD DISPLAY Aspiration Press (Ex: 15 x 50 μL) Ready...
PARAMETER SETTING Dilution mode, programming and pipetting In dilution mode, up to 3 volumes of different liquids (each separated by an air bubble) will be aspirated. The total resulting volume is then dispensed in a single dose. OPERATION ACTION KEY / BUTTON LCD DISPLAY Choice of dilution pipetting Select...
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PARAMETER SETTING Pipetting in dilution mode Press start button gently until first stop to work at the slow pipetting speed. Press fully (second stop) to work at the selected pipetting speed (fig. 2A). OPERATION ACTION KEY / BUTTON LCD DISPLAY Aspiration 1st volume Press Aspiration air bubble...
PARAMETER SETTING Dilution mode, interruption of pipetting sequence OPERATION ACTION KEY / BUTTON LCD DISPLAY Current display End of pipetting Press Blow out residual volume Double click Ready for new filling...
PARAMETER SETTING Tactile mode, programming In tactile mode, aspiration or pipetting is activated when start button is pressed. The process stops when button is released. It starts again by pressing start button. This mode is useful for liquid measurement/titration or gel loading. OPERATION ACTION KEY / BUTTON...
PARAMETER SETTING Tactile mode, pipetting – Titration or gel loading Press start button until second stop to aspire set volume. Press gently button until stop for dispens- ing. Release button to stop dispensing, or press again to continue distribution. OPERATION ACTION KEY / BUTTON LCD DISPLAY...
PARAMETER SETTING Mixing Available in any pipetting modes (exept tactile mode), the mixing performs consecutive back and forth aspiration/dispensing of the set volume. It is only possible after pipetting or purge steps are over. OPERATION ACTION KEY / BUTTON LCD DISPLAY Mixing with 3 cycles of Press 1 x aspiration/dispensing...
OPERATION Selection of pipetting speed (fig. 2B) Three pipetting speeds are available. Simply move the selector from left to right (slow/fast) to adapt working speed to the type of liquid or to a specific application. Independently from the speed selection, it is always possible to aspirate or distribute at the slow speed by gently pressing start button half way (feel first stop).
OPERATION Changing volumetric module ® Lower assembly of the Acura electro is interchangeable on a single control unit (upper assembly). Pipetting sequence must be completed before disassembling. Disassembling volumetric module Assembling up 2 mL (fig. 9 and 9a inside front cover) Remove protection cap and pull out plunger Press ejector button to the bottom Hold plunger between thumb and index...
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OPERATION Changing volumetric module (continued) Disassembling multichannel volumetric Assembling module (fig. 12 inside front cover) Remove protection cap Press ejector button Pull out plunger rod and introduce small pin Turn ejector nut to the left till its lowest (i.e. paper clip) in side hole to prevent from position, unclip ejector ring and release retracting ejector button...
OPERATION Programming of volumetric module When new volumetric module is inserted in control unit, user must enter corresponding parameters prior to start working. OPERATION ACTION KEY / BUTTON LCD DISPLAY Choice of volumetric module Last settings appear by default Ex : module ranging 20-200 μL Validate the choice Instrument performs a self-control...
OPERATION Correction of volumetric module selection (continued) OPERATION ACTION KEY / BUTTON LCD DISPLAY Correction of volumetric module (continued) Instrument performs self-control test automatically Select pipetting mode according to chapter “ Programming ” Warning: The programmed volumetric module must correspond to the one fitted on the pipette assembly. Use of Pasteur pipette (Acura 936, 2 mL and 5 mL) Glass Pasteur pipettes are of advantage in handling PP affecting solvents.
MAINTENANCE AND STERILISATION ® The Acura electro requires little maintenance. To obtain long term trouble free use, special attention during pipetting and regular cleaning are recommanded. Cleaning External parts of control unit, power handle On volumetric modules up to 1000 μL, and charging stands are cleaned with damped plunger tightness is provided by PTFE sleeve cloth...
MAINTENANCE AND STERILISATION Replacement of tightness parts (continued) Barrel change, multichannel volumetric modules Note : Tightness O-ring cannot be removed from barrel. Change barrel if tightness is deficient. Remove volumetric module from control unit Apply thin, even grease layer on all surface according to instructions in chapter «Opera- length of plunger before reassembling tion»...
INSTRUMENT CALIBRATION ® Each Acura electro pipette is factory tested for conformity according to ISO 8655 standards. Calibration parameters are permanently memorised in the instrument's micro processor. If performance results are no longer within recommended values, for instance after QC check, or replacing parts, or if changes occur in physical parameters (liquid density, temperature, atmospheric pressure), re-calibration is easily performed through the calibration menu.
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INSTRUMENT CALIBRATION New calibration When performance results are no longer within recommended values, a calibration should be performed using an analytical balance after insuring perfect working condition of the instrument. Proceed as follow for each calibration point. OPERATION ACTION KEY / BUTTON LCD DISPLAY Finish pipetting sequence Be sure desired...
TROUBLE SHOOTING Error messages LCD DISPLAY CAUSE SOLUTION Plunger sticking or dragging Disassemble volumetric module according to chapter “ Operation ” Clean plunger according to chapter “ Maintenance ” Reset instrument (see below) Plunger rod not clipped Reconnect plunger rod according step 5 and 6 of page 21 and 22.
TROUBLE SHOOTING Instrument reset (continued) OPERATION ACTION KEY / BUTTON LCD DISPLAY Or change calibration Press settings then Refer to chapter “ Calibration ” for new setting Other failures Observation Possible cause Action Power handle does not fit Control if compatible Exchange with 4.8V power handle pipette control unit 4.8V power handle...
PERFORMANCE Performance values obtained in Forward pipetting mode with bi-dist. water at constant tem- perature (± 0.5°C) between 20 and 25°C according to ISO 8655. ® electro 926 XS (reduced length) Acura Volume Division Inaccuracy (E%) Imprecision (CV%) Tip style μL μL Min.
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PERFORMANCE Performance values obtained in Forward pipetting mode with bi-dist. water at constant tem- perature (± 0.5°C) between 20 and 25°C according to ISO 8655. Acura ® electro 926 (regular length) Volume Division Inaccuracy (E%) Imprecision (CV%) Tip style Volumetric μL μL Min.
WARRANTY ® Your Acura electro and power handle are guaranteed against any material or manufacturing defects for the period of time specified in its QC certificate. Damages due to non-respect of manufacturer’s instructions, safety precautions or autoclaving conditions, as well as material colour alteration are excluded from the warranty.
ORDERING INFORMATION Micropipettes Initial package includes electronic pipette, individual QC certificat, two power handles, charging stand, power supply, accessories and operating instructions. Pipette alone is supplied with individual QC certificate, power handle, Qualitips ® pipette tips samples and operating instructions. ®...
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ORDERING INFORMATION Volumetric module – all models Volumetric Volume Tip style Cat. No. Volumetric Volume Tip style Cat. No. module module Reduced 0.1 – 2 μL Ultra 10 μL 800.0002XS Macro 0.1 - 2 mL 2 mL 800.2000 length 0.5 – 10 μL Ultra 10 μL 800.0010XS 0.25 - 5 mL 5 mL 800.5000...
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ORDERING INFORMATION ® Qualitips pipette tips Acura® electro 926 XS and 800 module Qualitips® compatibility chart microtips, 10 μL Natural ultra-microtip 309.0010B + R Natural ultra-microtip with filter 309.0010FR Natural microtip 302.0020B + R Natural microtip with filter 302.0010FR + AFB Natural gel loading tip 302.0010GR microtips, 20 μL...
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