Apogee MC-100 Owner's Manual
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Apogee MC-100 Owner's Manual

Chlorophyll concentration meter
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OWNER'S MANUAL

CHLOROPHYLL CONCENTRATION
METER
Model MC-100, U.S. Patent No. 9,733,179
APOGEE INSTRUMENTS, INC. | 721 WEST 1800 NORTH, LOGAN, UTAH 84321, USA
TEL: (435) 792-4700 | FAX: (435) 787-8268 | WEB: APOGEEINSTRUMENTS.COM
Copyright © 2020 Apogee Instruments, Inc.

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Summary of Contents for Apogee MC-100

  • Page 1: Owner's Manual

    OWNER’S MANUAL CHLOROPHYLL CONCENTRATION METER Model MC-100, U.S. Patent No. 9,733,179 APOGEE INSTRUMENTS, INC. | 721 WEST 1800 NORTH, LOGAN, UTAH 84321, USA TEL: (435) 792-4700 | FAX: (435) 787-8268 | WEB: APOGEEINSTRUMENTS.COM Copyright © 2020 Apogee Instruments, Inc.

  • Page 2: Table Of Contents

    TABLE OF CONTENTS Owner’s Manual ....................................1 Certificate of Compliance ................................. 3 Introduction ...................................... 4 Meter Models ....................................5 Specifications ....................................6 Theory of Operation ..................................7 Operation and Measurement ................................. 10 Installing new Crop-Specific Settings .............................. 19 Maintenance and Recalibration ..............................20 Troubleshooting and Customer Support ............................

  • Page 3: Certificate Of Compliance

    RoHS 3 compliant using exemption 6c. Further note that Apogee Instruments does not specifically run any analysis on our raw materials or end products for the presence of these substances, but rely on the information provided to us by our material suppliers.

  • Page 4: Introduction

    The MC-100 provides an alternative to destructive sampling techniques for determining chlorophyll concentration. It is less time consuming and labor intensive, and allows for leaves to be measured and monitored over an entire growth cycle.

  • Page 5: Meter Models

    METER MODELS The MC-100 chlorophyll concentration meter is the only chlorophyll meter model offered by Apogee Instruments. It includes custom coefficients to determine chlorophyll concentration for over 25 different plant species (with more coming soon). It also includes a generic equation for use with all plant species, where the equation was derived from the data from all twenty-two species measured.

  • Page 6: Specifications

    SPECIFICATIONS MC-100 Displayed Output Unit µmol of chlorophyll per m of leaf surface Measurement Area 63.6 mm (9 mm standard diameter), 19.6 mm (5 mm diameter with reducer) ± 10 µmol m chlorophyll concentration for generic equation (less for specific species), 0.1...

  • Page 7: Theory Of Operation

    700 nm, termed near infrared (NIR), is not used for photosynthesis. The Apogee Instruments MC-100 chlorophyll concentration meter exploits the different absorption characteristics of chlorophyll at different wavelengths to determine relative chlorophyll content. Radiation from two light...
  • Page 8 CCI value calculated from leaf transmittance spectrum in graph above), with larger ratios indicating more chlorophyll. The MC-100 chlorophyll concentration meter is unique in that it converts CCI, a relative measure of chlorophyll content, to an estimate of actual chlorophyll concentration. There is a non-linear relationship between CCI and chlorophyll concentration, and the relationship is different for different plant species (see relationship for three different crop species in figure below).
  • Page 9 (i.e., linearization of the output). Chlorophyll meters typically output an index that is non-linearly related to chlorophyll concentration (e.g., CCI or SPAD). The MC-100 outputs an estimate of actual chlorophyll concentration in units of µmol per m of leaf surface, thus changes in the displayed output are true changes in chlorophyll concentration.

  • Page 10: Operation And Measurement

    Press the power button to turn on the MC-100. After a few seconds the main menu screen appears. Program flow is organized into three menu groups. The selectable options are Run – used to set up and take measurements, Data –...
  • Page 11 MEASUREMENTS From the main menu select Run. Select Measure to perform measurements with the MC-100 and Setup to make adjustments to data files, test types, and measurement parameters. Select Back to return to the main menu. Run  Setup: Data Files The user can choose to save a data file to memory in addition to naming the file.
  • Page 12 RS-232. Run  Setup: Custom Measurements As detailed above, the MC-100 is capable of measuring in both units of CCI (relative chlorophyll content) and in µmol m (actual chlorophyll concentration). The default is to measure in units of CCI whereby the Use custom measurement units is set to No.
  • Page 13 Status: Release Arm Back The MC-100 requires a calibration when taking the first measurement after powering on the unit. Calibration is not required between measurements. Verify the sample chamber’s optical path is clear and hold down the measuring arm until the Release Arm message is displayed.
  • Page 14 MC-100 with serial numbers 1521 and above have a “GPS:” icon in the upper left corner of the screen to indicate the status of the GPS signal and location logging ability. A “-“ = GPS enable, no signal. A “+” = GPS enabled, signal acquired.
  • Page 15 Access Back Erase Connect MC-100 to a PC via USB cable or select Access to view data points on screen. Erase to delete data files. Data  Connect to PC USB Link Active! Connect the included USB cable from the MC-100 to a PC (note that any USB cable may be used that has a standard A plug to Mini-B plug).
  • Page 16 Edit By default, the MC-100 will beep every time a key is pressed, in addition to a four minute auto-off feature to help conserve battery power. To turn these features off, select Edit to highlight the Key press beep. Pressing the up and down arrows will toggle between Yes and No.
  • Page 17 For communication over RS-232 the appropriate baud rate will need to be set, otherwise USB should be selected. Select Edit to highlight the baud rate and use the up and down arrows to adjust. System  Diag: Meter Diagnostics The diagnostics section consists of five screens that detail system operation and overall performance. The information provided in the diagnostics section is intended for troubleshooting and/or repair.
  • Page 18 Using the Field of View Reducer for Small Leaf Applications: The field of view reducer (FOVR) accessory reduces the sample chamber area from 63.6 to 19.6 mm , roughly 30 % of the original size. Field of view reduction errors were less than 5 % for all leaves tested using the steps below.

  • Page 19: Installing New Crop-Specific Settings

    2. Click on one of the the “New Coefficient Zip Files” links to download. 3. Connect the micro USB end of a micro USB to USB cable (included with your meter) to your MC-100’s USB port. Connect the other end of the USB cable to your computer.

  • Page 20: Maintenance And Recalibration

    MAINTENANCE AND RECALIBRATION Moisture or debris on the MC-100 diffusers is a common cause of inconsistent readings. If cleaning is required, organic deposits are best removed using water or isopropyl alcohol and a soft cloth or cotton swab. Never use an abrasive material or cleaner on the diffusers.

  • Page 21: Troubleshooting And Customer Support

    9 V battery, holding down the power switch for 30 seconds, reinserting the battery and powering on the unit. If any of the fixes listed below do not rectify the problem contact Apogee Instruments technical support.

  • Page 22: Return And Warranty Policy

    RETURN AND WARRANTY POLICY RETURN POLICY Apogee Instruments will accept returns within 30 days of purchase as long as the product is in new condition (to be determined by Apogee). Returns are subject to a 10 % restocking fee. WARRANTY POLICY...
  • Page 23 84321, USA 5. Upon receipt, Apogee Instruments will determine the cause of failure. If the product is found to be defective in terms of operation to the published specifications due to a failure of product materials or craftsmanship, Apogee Instruments will repair or replace the items free of charge.

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