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Summary of Contents for ADInstruments Blood FlowMeter
- Page 1 Blood FlowMeter Laser Doppler Monitoring Owner’s Guide Blood FlowMeter Owner’s Guide...
- Page 2 Th is document was, as far as possible, accurate at the time of release. However, changes may have been made to the soft ware and hardware it describes since then. ADInstruments NZ Limited reserves the right to alter specifi cations as required. Late- breaking information may be supplied separately.
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Page 3: Table Of Contents
2 Setting Up Connecting the Blood FlowMeter ....... 18 Switching on the Blood FlowMeter ......19 Connecting the Laser Doppler Probe . - Page 4 A Care and Maintenance Care of the Blood FlowMeter ....... 24 Storage .
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Page 5: Safety Notes
ADInstruments products are NOT intended to be used as medical devices or in medical environments. Th at is, no product supplied by ADInstruments is intended to be used to diagnose, treat or monitor a subject. Furthermore no product is intended for the prevention, curing or alleviation of disease, injury or handicap. - Page 6 Safety Symbols Devices manufactured by ADInstruments that are designed for direct connection to humans are tested to IEC 601-1:1998 (including amendments 1 and 2) and 60601-1-2, and carry one or more of the safety symbols below. Th ese symbols appear next to those inputs and output connectors that can be directly connected to human subjects.
- Page 7 • All Bio Amp front-ends and PowerLab units with a built-in Bio Amp are not defi brillator-protected. Using the Bio Amp to record signals during defi brillator discharges may damage the input stages of the amplifi ers. Th is may result in a safety hazard. •...
- Page 8 Th erefore, under no circumstances should any other transformer be used with the Stimulus Isolator. For a replacement transformer plug pack please contact your nearest ADInstruments representative. General Safety Instructions To achieve the optimal degree of subject and operator safety, consideration...
- Page 9 While it is not possible to cover all arrangements of equipment in a system, some general guidelines for safe use of the equipment are presented below: • Any electrical equipment which is located within the SUBJECT AREA should be approved to IEC60601-1. •...
- Page 10 Cleaning and Sterilization ADInstruments products may be wiped down with a lint free cloth moistened with industrial methylated spirit. Refer to the Data Card supplied with transducers and accessories for specifi c cleaning and sterilizing instructions.
- Page 11 Equipment (WEEE) Directive symbol requires separate waste collection. For a product labeled with this symbol, either forward to a recycling center or contact your nearest ADInstruments representative for methods of disposal at the end of its working life. WEEE Directive...
- Page 12 Blood FlowMeter Owner’s Guide...
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Page 13: Introduction
C H A P T E R Introduction Th e ADInstruments Blood FlowMeter is one of a family of stand-alone instruments, designed to measure blood cell perfusion levels in the microcirculatory beds of skin and other tissues. Th is Blood FlowMeter... -
Page 14: How To Use This Guide
Th is owner’s guide describes how to set up and begin using your Blood FlowMeter. Topics discussed included how to connect the hardware, perform a simple power-up test and calibration of the Blood FlowMeter. Th e appendices provide technical information about the Blood FlowMeter and look at some potential problems and their solutions. -
Page 15: Power Indicator
Probe connected and laser operating Probe Input Connector Th e Blood FlowMeter is a single channel device and provides one probe input connector. Th e input connector is a polarized (keyed) multi-pin connector with optical interfaces for the fi ber optics in the probe cable. Th e probes are designed to push and click into the connector. -
Page 16: Usb Port (Lcc/Digital)
AC supplies. Connection of power valid when used in conjunction with the to the Blood FlowMeter is via a mini DIN connector. All power at this point power supply provided. is low voltage. -
Page 17: Setting Up
CD. Follow the onscreen instructions. Next, install the Blood FlowMeter Device Enabler Th e Blood FlowMeter Device Enabler is a soft ware add-on for LabChart soft ware. Place the CD for the ADInstruments Blood FlowMeter Device Enabler in the CD drive of your computer. -
Page 18: Connecting The Blood Flowmeter
Blood FlowMeter are turned off . Connect the power supply output of the external supply to the power input of the Blood FlowMeter. Make sure that this connector is pushed all the way in, as it does not have a locking facility. -
Page 19: Switching On The Blood Flowmeter
When the power has been connected, turn on the power switch on the rear panel of the Blood FlowMeter. Th e Power indicator on the front of the Blood FlowMeter should glow green and you should hear a double beep from the unit to indicate that it has initialized. -
Page 20: Probe Application
+ 5 V (+100 %) Temperature Warning If the internal temperature of the Blood FlowMeter moves outside the operating temperature range of the device, then it will emit a double beep every 16 seconds. If this occurs, the instruments should be moved to a cooler environment for proper operation. -
Page 21: Probe Calibration
Probe Calibration Th e Blood FlowMeter is designed to be easily confi gured and calibrated for new probes. Th is operation requires a MLA191 Calibration Kit, which includes a controlled concentration of latex spheres in suspension. It is important to follow the instructions provided with the Calibration Kit as calibration of probes is not possible without it and should not be attempted. -
Page 22: Using The Blood Flowmeter With Labchart
Th e following sections describe how to use the Blood FlowMeter with a PowerLab data acquisition unit (BNC connection) and LabChart soft ware. It is assumed that you know how to connect the Blood FlowMeter to your PowerLab system and that you are familiar with the LabChart soft ware. -
Page 23: Removing The Probes
An example of what you should see is shown in Figure 2–3. Th is fi gure also shows a typical waveform from the LDF output. You can use a fi lter Low pass setting to remove any unwanted noise. You should not use the AC coupled feature as this will result in an incorrect measurement of blood perfusion units. -
Page 24: A Care And Maintenance
Cleaning Warning: Do not spray, pour or spill any liquid on the Blood FlowMeter, its accessories, connectors, switches or openings in the enclosure. Do not submerse the instrument in liquid. -
Page 25: Storing The Probes
Th e probe connectors must be kept clean and free from dust. Connectors should be inspected before each use. Dust can be removed from the connectors using an air-duster. Th e integrity of probes may be checked by holding the probe end to a source of bright diff use light, for example a lamp, and inspecting the connector end. -
Page 26: Disinfection
• 70% alcohol in water Th e disinfectant manufacturer’s recommended immersion times should be used. Sterilization Only some of the dedicated perfusion probes may be sterilized by moist heat (steam). For further details please contact your ADInstruments representative. Blood FlowMeter Owner’s Guide... - Page 27 Appendix A Care and Maintenance...
- Page 28 In the majority of cases, the problem can be fi xed by checking connections and or resetting the Blood FlowMeter. Very rarely will there be an actual problem with the Blood FlowMeter. Th is appendix will help you to determine what kind of fault you have and an appropriate solution.
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Page 29: B Troubleshooting
Contact your ADInstruments representative for help. Th e Blood FlowMeter does not produce any laser output when the power is A faulty probe connection. • Make sure the probe is properly connected to the system by pushing its connector fi rmly into the front panel socket. - Page 30 Th ere is no signal output from the Blood FlowMeter No power to the Blood FlowMeter. • Check that the Power indicator on the Blood FlowMeter is on. Th e probe is not plugged in correctly. • Check the probe is connected correctly.
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Page 31: C Technical Aspects
A P P E N D I X Technical Aspects Blood fl ow in the skin performs an essential role in the regulation of the metabolic, hemodynamic and thermal state of the individual. Despite the fact that the skin is the body’s largest and most accessible organ, the measurement of cutaneous microcirculatory blood perfusion has until quite recently proved a formidable task. -
Page 32: Laser Doppler Flowmetry
Th is is performed using a photo diode detector. Th e frequency and magnitude of the alternating component of the photocurrent from this device (i.e. the power spectral density) is related to the mean velocity and concentration of blood cells present in the measuring volume. Blood FlowMeter Owner’s Guide... -
Page 33: How It Works
LDF signal. How it Works Th e Blood FlowMeter determines the blood fl ow perfusion by illuminating the surface of the skin with laser light and measuring the backscattered light. Various calculations are then performed on the returned signal which is then turned into a continuous signal that represents the blood perfusion in the illuminated area. -
Page 34: Probe Operation
What the Blood FlowMeter Measures Th e Blood FlowMeter is a laser Doppler fl owmeter whose primary purpose is to measure real-time microvascular red blood cell (RBC) perfusion (otherwise known as RBC fl ow or RBC fl ux). Laser Doppler signals are recorded in BPU which is a relative units scale defi ned using a carefully controlled motility standard. -
Page 35: Zero Bpu
BPU must be considered as strictly relative. Zero BPU Th e zero reading of the Blood FlowMeter has been obtained by calibrating the system against a special static scattering material where no movements occur. In such cases the back-scattered light processed by the Blood FlowMeter contains no Doppler shift ed frequencies and a true zero is obtained. - Page 36 Under these conditions, artifacts should be minimized by controlling vibration reaching the measurement site and the Blood FlowMeter. Th is may be achieved by placing the ventilator on a separate table and on a vibration absorbing medium. Blood FlowMeter Owner’s Guide...
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Page 37: D Specifi Cations
A P P E N D I X Specifi cations Doppler Flow Specifi cations Mode: Continuous laser Doppler fl owmetry Primary measurand: Microvascular blood perfusion (Relative RBC Flux) LDF units: Relative units (0-5000 Blood Perfusion Units, corresponding to 0-5 V output) BSC units: Relative units (0-100% corresponding to 0-5 V output) -
Page 38: Power Supply
(2.70" × 9.45" × 10.2") Weight: 2.5 kg Power requirements: 240 V or 120 V (internally set) @ 15 W Operating temperature: 5-35 °C Operating humidity: 0-70% (non-condensing) ADInstruments reserves the right to alter these specifi cations at any time. Blood FlowMeter Owner’s Guide... -
Page 39: Index
LDF theory 32 limitations 34 maintenance 10 zero readings 35 microvascular perfusion 32 BSC output 32 motion artifacts 35 checking the Blood FlowMeter 14 optic fi ber 33 cleaning 10, 24 connecting probes 18 connectors power indicator light 15 back panel 15... - Page 40 34 removing probes 23 Safety Notes 5–11 signal connection 18 signal outputs 15 sterilization of probes 26 storage 11 storage of probes 25 technical aspects 31 troubleshooting 28 using this guide 14 zero BPU readings 35 Blood FlowMeter Owner’s Guide...
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