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DMT MYOGRAPH SYSTEM 112PP User Manual

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USER
MANUAL, VOL.2.0
M Y O G R A P H S Y S T E M - 1 1 2 P P / 1 1 4 P

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Summary of Contents for DMT MYOGRAPH SYSTEM 112PP

  • Page 1 USER MANUAL, VOL.2.0 M Y O G R A P H S Y S T E M - 1 1 2 P P / 1 1 4 P...

  • Page 3: Trademarks

    New information may be supplied separately. This documentation is provided with the DMT Automated Multi Wire Myograph System – Model 630MA All rights reserved. No part of this manual may be reproduced or transmitted in any form or by any means without the written permission of Danish Myo Technology A/S.

  • Page 4: Table Of Contents

    CONTENTS TRADEMARKS SAFETY UNPACKING THE PRESSURE MYOGRAPH SYSTEM CHAPTER 1 - SYSTEM OVERVIEW 1.1 Interface Front Panel 1.2 Pressure Myographs 2.1 Setting up the complete Pressure Myograph System 2.2 Setting up step-by-step 2.3 The first force and pressure calibrations 2.4 External Pressure Myograph connections CHAPTER 3 - PRESSURE INTERFACE MENUS 3.1 General description of how to navigate the touch screen 3.2 Power-up screen...

  • Page 5: Safety

    SAFETY The Pressure Myograph System has been designed for use only in teaching and research applications. It is not intended for clinical or critical life-care use and should never be used for these purposes. Nor for the prevention, diagnosis, curing, treatment, or alleviation of disease, injury, or handicap. CAUTION: •...
  • Page 6 EC DECLARATION OF CONFORMITY DMT A/S Certify and declare that the following apparatus: Pressure Myograph System - DMT114P, DMT112PP Restrictive use: Only for laboratory use. Manufactured by: DMT A/S Skejbyparken 152 8200 Aarhus N. Denmark Conforms with the essential requirements of the EMC Directive 2004/108/EC.

  • Page 7: Unpacking The Pressure Myograph System

    Myograph System. In case of transport, and the original packing material is unavailable, please contact the DMT Sales Department for advice and packing instructions. After unpacking your new Pressure Myograph System, please use the following list to check that the system is complete: Pressure Interface •...
  • Page 8 Accessories: • Allen key • Spare screws • Small screwdriver • Suture wire • Tube of high-vacuum grease • Tube of grease for linear slides • Silicone tubing: internal diameter 1mm Computer, hardware, software & manuals: • USB digital camera (optional) •...

  • Page 9: Chapter 1 - System Overview

    CHAPTER 1 - SYSTEM OVERVIEW 1.1 INTERFACE FRONT PANEL Touch display 2 x 500 ml Schott Duran bottles Power indicator Figure 1.1 Pressure Interface - front...
  • Page 10 2 x 500 ml Schott Duran bottles Ground connection Inlet for oxygen/carbogen to pressure bottles (max 1 bar) 24V DC port for power Connection port for pH electrode (optional) ON/OFF switch USB connection port for Connection port for Myograph chamber computer Analogue recorder output – connection to external data acquisition software (optional) Figure 1.2 Pressure Interface - rear...

  • Page 11: Pressure Myographs

    1.2 PRESSURE MYOGRAPHS 1.2.1 PRESSURE MYOGRAPH - 114P Micropositioner for horizontal adjustment of Micropositioner for vertical Glass Cannulas adjustment of Glass Cannulas Outlet pressure transducer Force Transducer housing 25 pin serial port for connection pipe communication with Pressure Interface 3-way Inlet valve Inlet pressure Outlet pressure Force transducer pin 3-way Outlet valve transducer transducer...
  • Page 12 1.2.2 PULSATILE PRESSURE MYOGRAPH - 112PP Micropositioner for vertical Micropositioner for adjustment of Glass Cannulas horizontal adjustment of Glass Cannulas Outlet pressure transducer Force Transducer housing connection pipe 25 pin serial port for communication with Pressure Interface 3-way Inlet valve Pulsation Pulsation Force transducer pin Chamber 1 3-way Outlet valve Chamber 2 Outlet pressure transducer...

  • Page 13: Setting Up The Complete Pressure Myograph System

    In addition, the overview also includes the DMT Flowmeter - 162FM. The Flowmeter enables flow measurements in the range of 15-4000μl/min. A detailed step-by-step description is provided in the remaining sections of chapter 2.
  • Page 14 NOTE: The microscope is essential for the dimensional analysis of the mounted vessels The DMT Microscope is a standard inverted microscope with a built-in digital CCD USB camera. The DMT Microscope is very effective and recommended in setups requiring standard image analysis.

  • Page 15: Setting Up Step-By-Step

    4. Microscope/camera to computer connection: A. DMT Inverted Microscope: Inside the DMT Microscope there is a USB camera wired to a USB cable and one USB cable to power the microscope light. Connect the two USB cables from the DMT Microscope to the computer USB ports.
  • Page 16 NOTE: If you have purchased a computer from dmt with your pressure myograph system then myoview data, acquisition software has already been installed with drivers for the digital usb camera. Follow the next procedure to setup the pressure myograph system NOTE: If you have not purchased a computer from dmt follow the myoview quick installation guide to install myoview on your own computer 5. Pressure Interface to external Data Acquisition System connection (optional): If you have not obtained...

  • Page 17: The First Force And Pressure Calibrations

    However in order to ensure that the Pressure Myograph System is working at highest performance, DMT recommends that new force and pressure calibrations are performed before the first use. The force and pressure calibration procedures are described in detail...

  • Page 18: External Pressure Myograph Connections

    Perfusion outlet P2 Suction pipe to vacuum Extra Perfusion inlet P1 Extra Perfusion inlet Superfusion Access hole for Extra Perfusion inlet pipe temperature or pH outlet Access hole for probe adding buffer and drugs Figure 2.1 Example of a complete set-up of Pressure Myograph System with DMT inverted Microscope...
  • Page 19 Perfusion inlet P1 Superfusion outlet Suction pipe for vacuum Inlet pipe for Oxygen/ carbogen supply Perfusion outlet P2 Extra Perfusion inlet Extra Perfusion outlet Access hole for adding Access hole for buffer and drugs temperature or pH probe Superfusion inlet pipe Figure 2.3 External Pressure Myograph connections for 112PP Figure 2.4 Pressure Interface tube connections with Pressure Myograph Example with Pressure Myograph - 114P...
  • Page 20 NOTE: To fill the tubing and avoid air bubbles, mount the calibration shunt (resistance tube) as shown below and described in chapter 3.7.2.1. Inlet pipe for Oxygen/ carbogen supply Calibration Shunt tube (delivered in a small box with the system) Figure 2.5 Mounting of the Calibration Shunt tube in the 114P and 112PP chamber...
  • Page 21 Fill the p1 schott bottle with a maximum of 300 ml of buffer. In the pressure interface menu set the pressure p1 and p2 as follow: P1 = 150mm Hg P2 = 70mm Hg Set the Pressure ON Set Flow ON FOR 114P USERS CONTINUE AS BELOW AND FOR 112PP, USERS GO TO SECTION 9 IMPORTANT: •...
  • Page 22 Detach the silicone from the short steel pipe here and attach a 5ml syringe with an 18-gauge needle. 4. Attach a 5ml syringe with a 18-gauge needle to the short silicone tubing on the P1 glass cannula and use the syringe to drag buffer into the P1 glass cannula and silicone tube. When all air is removed, re-connect the short silicone tube to the steel pipe inside the chamber again.
  • Page 23 7. Inspect the glass cannulas and the silicone tubing in the chamber for air bubbles using a dissection microscope. If no air bubbles are visible then continue with the mounting of the artery (See USER GUIDE). If not the try to repeat the above until all air bubbles are removed. 8.
  • Page 24 FOR 112PP USERS CONTINUE AS FOLLOWS 12. Let the pressure regulator run and open the valve into the P1 Pulsation Chamber (see figure below). As soon as the bottom part of P1 pulsation chamber is filled with buffer, quickly close the valve again. NOTE: Do not fill the pulsation chamber completely, only the bottom area otherwise, the pulsation will not work properly 13.
  • Page 25 Detach the silicone from the short steel pipe here and attach a 5ml syringe with an 18-gauge needle. 17. Attach a 5ml syringe with an 18-gauge needle to the short silicone tubing on the P1 glass cannula and use the syringe to drag buffer into the P1 glass cannula and silicone tube. When all air is removed, re-connect the short silicone tube to the steel pipe inside the chamber again.
  • Page 26 20. Inspect the glass cannulas and the silicone tubing in the chamber for air bubbles using a dissection microscope. If no air bubbles are visible then continue mounting the artery. If not then try to repeat the above until all air bubbles are removed. 21.
  • Page 27 Creating a superfusion flow in the Pressure Myograph chamber requires an external peristaltic pump, which is not part of the basic Pressure Myograph System. Contact DMT for further information about recommended perfusion pump models, specifications and prices. The superfusion circuit is connected...
  • Page 28 FlowMeter as soon as it is connected to the Pressure Interface and will be ready for use immediately. The External DMT 162FM Flowmeter is connected at the P2 side of the 114P/112PP chamber using the applied silicone tubing with the internal diameter of 1mm. Connect the P2 outlet from the 114P/112PP chamber with the DMT 162FM Flowmeter Inlet and then connect the DMT 162FM Flowmeter outlet with the P2 connection on the Pressure Interface (see in figure below).
  • Page 29 2.4.6 CONNECTING A SUCTION DEVICE Connect the “large” pipe on the Pressure Myograph chamber cover (see figure 2.7) to a vacuum pump via a suction bottle and a vacuum valve as illustrated in figure 2.1. The internal diameter of the silicone tube used for this connection must be 2 mm. (a 2 mm tube is not included with the Pressure Myograph System).

  • Page 30: Chapter 3 - Pressure Interface Menus

    CHAPTER 3 - PRESSURE INTERFACE MENUS This chapter contains a detailed description of how to navigate the touch screen menus and how to use the special features of the Pressure Interface. 3.1 GENERAL DESCRIPTION OF HOW TO NAVIGATE THE TOUCH SCREEN Menus on the Pressure Interface are all accessible by a touch CH1  ARTERIES  PARAMETER screen.

  • Page 31: Power-Up Screen

    3.2 POWER-UP SCREEN After turning on the Pressure Interface, an introduction screen appears. It displays the software version number. While this screen is displayed the system is auto-initializing. After a few seconds, the Main Menu display will appear.

  • Page 32: Main Menu

    3.3 MAIN MENU The Main Menu gives a good overall picture of how the Pressure Interface is working. It displays values for the input pressure, output pressure, force, pH, probe temperature and time, and the status of systems including the time, heat, flow and pressure. See next page for a description of the Main Menu. Four sub-menus are accessible from the Main Menu screen: Pressure Menu Heat Menu, Timer and Buzzer Menu Setting Menu Icon for Pressure ON/OFF...
  • Page 33 Buzzer icon: This icon indicates the status on the buzzer. If the icon is grey the buzzer is OFF. If the icon is green the buzzer is ON (active) and will make a sound when the timer reaches zero. Timer: 00:00:00 The Timer is a countdown timer that can be set to maximum 24 hours.

  • Page 34: Pressure Menu

    3.4 PRESSURE MENU The pressure is controlled from this menu. Use SELECT to select the line to be changed. When selected the line changes to blue (edit mode). Use DEFAULT or UP and DOWN arrows to change the setpoint value. Pressing ENTER will save the new setpoint.
  • Page 35 The pressure regulator and the flow are also controlled from this menu. To turn the pressure regulator on press ON outside the line for Pressure. When activated it turns green. In Main Menu the pressure icon also turns green when pressure regulation is on.

  • Page 36: Heat Menu

    3.5 HEAT MENU The chamber and bottle heating temperature are controlled from this menu. To turn the heat on, or to change the temperature for the system, press the HEAT in the Main Menu. The display will enter the Heat Menu and allow the user to change the system temperature, as well as turn heat on or off.

  • Page 37: Timer And Buzzer Menu

    3.6 TIMER AND BUZZER MENU The timer and buzzer are controlled from this menu. Use SELECT to program the timer. The timer can be programmed in intervals from 0 to 23:59:59 (Hours : minutes : seconds). When Set Timer is selected press the UP and DOWN arrows to program the timer.

  • Page 38: Setting Menu

    Prior to shipping, the Pressure Myograph System has gone through two days of continuous testing, including final force calibrations. However, DMT recommends that new force calibrations are performed before starting to use the Pressure Myograph System for the first time. The calibration procedure needs to be performed to calibrate the system.
  • Page 39 Enter Force Calibration Menu by pressing Settings in the Main Menu and then press Force Calibration Menu to begin the transducer calibration. The display will show the force calibration procedure. The calibration procedure is listed in 6 individual steps and needs to be performed one at a time to calibrate the system.
  • Page 40 Figure 3.1 - Illustration of how to fit the T-balance just behind the P2 Pipette holder arm...
  • Page 41 Step 3 - This step initiates the heating process for the chamber. In order for the calibration to be accurate, the transducer must be heated to the same temperature as used in your experiments. This allows for heat-induced expansion of the electronic parts in the transducer.
  • Page 42 Step 6 - This step verifies that the calibration was performed correctly. The Force Chamber reading should be 9.81 ± 0.1 mN. If the Force Chamber reading is off by more than 0.1 mN then remove the weight, press BACK to return to step 4, and repeat the calibration process.
  • Page 43 PRESSURE CALIBRATION PROCEDURE FOR PRESSURE MYOGRAPH 114P 1. Connect the Pressure Interface with the Pressure Myograph. 2. Connect the Big Ben Pressure Manometer to the 3-way valve at P1 on the Pressure Myograph as illustrated in figure 3.2. 3. Inside the Pressure Myograph chamber, on the right hand side, is a small stainless steel pipe, which is connected to the right glass cannula via a small silicone tube “B”, see figure 3.3.
  • Page 44 6. Close the inlet from the Pressure Interface o P1 by turning the handle on the 3-way valve towards the normal inlet way, see figure 3.5. The inlet to P1 now comes from the pressure manometer and the Pressure Myograph system is now ready for a calibration procedure. Figure 3.5 Three-way valve flow settings at P1 3.7.2.2 Set-up the pressure calibration on the Pressure Interface for the 114P System Press Settings in the Main Menu and then press Pressure Calibration.
  • Page 45 Step 2 - Apply a pressure of 50mmHg on the Pressure Myograph using the Big Ben Pressure Manometer. When the 3 blue lines of relative values stabilize, go to NEXT STEP. The relative values should be between 3300 to 3900 Step 3 - Apply a pressure of 125mmHg to the Pressure Myograph using the Big Ben Pressure Manometer.
  • Page 46 3.7.2.3 Set-up the pressure calibration on the Pressure Interface for the 112PP System Step 1 - Press NEXT STEP when the system is ready. Step 2 - Apply a pressure of 50mmHg on the Pressure Myograph using the Big Ben Pressure Manometer. When the 4 blue lines of relative values stabilize, go to NEXT STEP.
  • Page 47 Step 5 - The calibration is now finished. The readings in the four bottom lines are the actual pressure on all four pressure transducers. The values must be 200.0 +/-1.0mmHg. Carefully remove the calibration shunt tube and reconnect the tubing to the glass cannulas. 3.7.3 PH CALIBRATION PROCEDURE (OPTIONAL) Before the pH calibration is performed be sure to select the...
  • Page 48 Step 2 - Place the pH electrode and temperature probe in the high buffer solution (here pH 7) and turn on stirring of the high buffer solution. When the relative pH output in the blue line is stable, go to NEXT STEP. Step 3 - Place the pH electrode and temperature probe in the low buffer solution (here pH 4) and turn on stirring of the low buffer solution.
  • Page 49 3.7.4 SELECT ANALOG OUTPUT (OPTIONAL) The Analog Output Menu determines which data is sent to the BNC analog outputs (REC 1, REC 2 REC 3, REC 4) at the back of the Pressure Interface, four in total. Each output is individually programmable. Any changes made to the analog output will only affect the data collected by the external data acquisition system.
  • Page 50 3.7.5. INTERFACE SETTINGS The Interface Settings sub-menu in Settings has an additional three sub-menus which include: • Temperature Difference • pH Set-up • Factory Diagnostics 3.7.5.1 Temperature Difference (offset) The temperature difference function allows the user to fine- tune the temperature setpoint of the system. Although the temperature setpoint for the system can be set in the Heat Menu, the actual temperature for the system may not heat to the exact setpoint.
  • Page 51 3.7.5.3 Factory Diagnostics Entering Factory Diagnostics will display the Login code to diagnostics window. This window is for trained technicians and is used for diagnostics and troubleshooting purposes. The general user will not have access to this window. However, entering the proper five-digit login code, will give the trained technician access to diagnostics panels that will provide information during a malfunction, or mechanisms to change other settings controlled by the on-board computer.
  • Page 52 3.7.7. PULSE RATE (ONLY FOR PULSATILE 112PP SYSTEM) The Select Pulse Rate Menu is only for users that have the Pulsatile Pressure 112PP chamber/system. In the Pulse Rate menu, the number of pulses per minute can be set between 50 and 600 Pulses per minute. By selecting 400 Pulse/min, the Pressure Interface will pulse between the P1 and P2 pressure 400 times a minute.
  • Page 53 When the Pressure exceed 200mmHg the number will, turn red to indicate that the pressure applied manually is close to the maximum pressure the system can handle without breaking the pressure transducers.
  • Page 54 PRESSURE MYOGRAPH SYSTEM - 112PP CHAMBER: Chamber volume (min) 3.1 ml Chamber(s) Chamber material Acid resistant stainless steel Vessel size >40 µm Vessel alignment X, Y, Z Micrometer resolution 0.01 mm Mounting type Cannulas Pulse rate 50 - 600 BPM TEMPERATURE: Range 15.0 to 50.0 °C Resolution 0.1 °C Stability...
  • Page 55 PRESSURE MYOGRAPH SYSTEM - 114P CHAMBER: Chamber volume (min) 3.1 ml Chamber(s) Chamber material Acid resistant stainless steel Vessel size >40 µm Vessel alignment X, Y, Z Micrometer resolution 0.01 mm Mounting type Cannulas TEMPERATURE: Range 15.0 to 50.0 °C Resolution 0.1 °C Stability ±0.2 °C Heating TRANSDUCER FORCE:...
  • Page 56 Danish Myo Technology A/S DMT-USA,Inc. E-mail: sales@dmt.dk E-mail: sales@dmt-usa.com T el.: +45 87 41 11 00 Tel.: +1 734 707 0250 Fax: +45 87 41 11 01 Fax: +1 678 302 7013 DMT-China DMT-Asia Pacific T el.: 86-21-6486 9685 Tel.: +61 2 8814 1597 Fax: 86-21-5877 0063...

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Myograph system 114p

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