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W arning: This product contains chemicals, including triglycidyl isocyanurate, known to the State of 4 8 T 4 8 T California to cause cancer as well as birth defects or other reproductive harm. For more information, go to w ww.P65Warnings.ca.gov 3 3 T 3 3 T ¡...
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220 – 240 Voltage Part Number (Manual): 4861733 Revision: May 7, 2020 SHEL LAB is a brand of Sheldon Manufacturing, INC, an ISO 9001 certified manufacturer. Safety Certifications These units are TÜV listed as water jacket incubators for professional, industrial, or educational use where the preparation or testing of materials is done at an ambient air pressure range of 22.14 –...
TABLE OF CONTENTS INTRODUCTION ..............................7 Read this Manual ..................................7 Safety Considerations and Requirements ........................7 Contacting Assistance ................................8 Manufacturing Warranty ..............................8 Engineering Improvements ..............................8 Gas Supply ..................................9 Reference Sensor Devices ..............................10 RECEIVING YOUR UNIT ............................11 Inspect the Shipment ................................
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Calibrate the CO Display ..............................51 Anode and Water Quality ..............................55 UNIT SPECIFICATIONS ............................57 Weight ......................................57 Dimensions .....................................57 Max Shelves For Unit ................................. 58 Capacity ....................................58 ......................................58 Temperature ..................................58 Power ...................................... 58 PARTS LIST ................................59 Accessories ...................................
INTRODUCTION Thank you for purchasing a SHEL LAB incubator. We know you have many choices in today’s competitive marketplace when it comes to constant temperature equipment. We appreciate you choosing ours. We stand behind our products and will be here if you need us.
ONTACTING SSISTANCE Phone hours for Sheldon Customer Support are 6 am – 4:30 pm Pacific Coast Time (west coast of the United States, UTC -8), Monday – Friday. Please have the following information ready when calling or emailing Customer Support: the model number, serial number, and part number (see page 13).
INTRODUCTION Note: A CO gas regulator must be purchased separately from the incubator. UPPLY Supply Required The incubator must be connected to a carbon dioxide gas supply system in order to establish and maintain a CO -enriched incubation chamber atmosphere. The supply can be a building CO gas system or one or two supply cylinders (tanks).
INTRODUCTION EFERENCE ENSOR EVICES Must be purchased separately Reference sensor devices or a combined device are required for calibrating the incubator temperature and CO displays. Reference devices must meet the following standards: Accurate to at least 0.1°C • Accurate to at least 0.1% CO •...
RECEIVING YOUR UNIT NSPECT THE HIPMENT When a unit leaves the factory, safe delivery becomes the responsibility of the carrier. • Damage sustained during transit is not covered by the manufacturing defect warranty. • Save the shipping carton until you are certain that the unit and its accessories function •...
RECEIVING RIENTATION MAGE SCO5W-2 Back of Unit Back of Unit Side of Unit 2nd Fuse in Sample Water Jacket Ports Holder Fuse in Power Inlet Fill and Data Ports Siphon Anode In Ports Access Port Chamber Door Handle Control Panel Door Gasket Chamber Gasket Door Latch...
RECEIVING ECORD LATE NFORMATION The data plate contains the unit model number, serial number, and part number. Customer Support will need this information during any support call. Record it below for future reference. • The data plate is located on the inside of the incubation chamber door in the top right corner. Data Plate Information MODEL NO: SERIAL NO:...
INSTALLATION NSTALLATION ROCEDURE HECKLIST For installing the unit in a new workspace location. Pre-Installation Verify a CO gas supply source suitable for your application is available and can be connected to the incubator, page 9. Check that the required ambient conditions for the unit are met, page 16.
INSTALLATION EQUIRED MBIENT ONDITIONS These units are built for use indoors at room temperatures between 15°C and 30°C (59°F and 86°F), at no greater than 80% Relative Humidity (at 25°C / 77°F The ambient temperature should not change by 2°C (3.6°F) or more during operation. Operating outside these conditions may affect the incubator temperature performance.
INSTALLATION OWER OURCE EQUIREMENTS When selecting a location for the unit, verify each of the following requirements is satisfied. Power Source: The power source must match the voltage and amperage requirements listed on the unit data plate. This unit is intended for 220 – 240 volt, 50/60 Hz applications at 3.5 amps. The wall power source must be protective earth grounded.
INSTALLATION IFTING AND ANDLING The unit is heavy. Use appropriate lifting devices that are sufficiently rated for these loads. Follow these guidelines when lifting the unit. Lift the unit only from its bottom surface. • Doors, handles, and knobs are not adequate for lifting or stabilization. •...
INSTALLATION NSTALL THE NCUBATOR Install the unit in a workspace location that meets the criteria discussed in the previous entries of the Installation section. EIONIZED AND ISTILLED ATER Do not use deionized water to clean the unit, even if DI water is readily available in your laboratory. Use of deionized water may corrode metal surfaces and is not covered by the •...
INSTALLATION HEPA F NSTALL HAMBER ILTER AND EILING The incubator must be unplugged while carrying out this procedure. Exercise caution when installing the ceiling air duct. A plastic blower fan and the fragile head of the temperature and CO sensors are located just above the duct and just to the right of the duct. Step 2: Attach cap to filter Step 1: Attach filter to duct Ceiling Duct: Flipped for ease of...
INSTALLATION HELVING NSTALLATION Install the shelving in the incubation chamber. Install the shelf standard rails. • Align the keyhole slot of the standard with the mounting peg on the side of the chamber wall. • Mount the shelf standard. Install the shelf slides. •...
INSTALLATION ONNECT TO THE UPPLY Note: See page 9 for CO supply requirements. to Chamber Dual-Stage CO Optional Tubing Kit Connects to 3/16” Pressure Regulator with Inline HEPA Filter (4.76mm) ID Tubing Single Source Dual Source “IN” “IN” Connect the supply source to the incubator. If connecting a second supply cylinder, do so now.
INSTALLATION CCESS TOPPER Each incubator ships with a silicone stopper installed in the access port located on the right side of the incubation chamber interior. The stopper should always be installed inside the chamber to obtain the • best temperature uniformity and prevent condensation from forming inside the port.
GRAPHIC SYMBOLS The unit is provided with graphic symbols on its exterior. These identify hazards and adjustable components as well as important notes in the user manual. Symbol Definition Consult the user manual Consulter le manuel d'utilisation Temperature display Indique l'affichage de la température Over Temperature Limit system Thermostat température limite contrôle haute AC Power...
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SYMBOLS Symbol Definition Potential shock hazard Risque de choc électrique Recycle the unit. Do not dispose of in a landfill. Recycler l'unité. Ne jetez pas dans une décharge. Indicates relative humidity Indique humidité relative Indicates water level low Indique faible niveau d'eau Indicates CO Indique gaz CO2 Indicates the incubator heater is active...
CONTROL PANEL OVERVIEW Power Switch Power is supplied when the switch is in the ( I ) on position. Control Panel Power Switch Power is supplied when the switch is in the ( I ) ON position. Over Temperature Limit This graduated dial sets the mechanical heating cut off for the Over Temperature Limit system (OTL).
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CONTROL PANEL OVERVIEW Set CO Display and Controls Shows the current concentration of CO as a percentage of the incubation chamber atmosphere. The display has a range of OFF (0%) to 20% and an accuracy of 0.1%. The display shows “LO” until the CO sensor registers a concentration in the chamber greater than 0%.
OPERATION HEORY OF PERATION Heating When powered, the incubator heats to and maintains a user-selected target setpoint in the incubation chamber. The incubator senses the chamber air temperature using a solid-state probe mounted on the chamber interior wall. When the incubator detects that the chamber temperature has dropped below the target setpoint, it pulses power to heating elements on the surface of the water-jacket insulation space and in the outer chamber door.
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OPERATION Atmosphere The incubator also controls and maintains the gas concentration of CO in the chamber atmosphere by operating an internal injection solenoid valve connected to a gas input port. The incubator monitors CO concentration level in the chamber using an infrared sensor located in the recirculation duct.
OPERATION Note: From a cold start, the incubator requires 12 hours to come up to and stabilize at temperature and humidity levels prior to loading samples. Stabilization safeguards samples. UT THE NCUBATOR INTO PERATION Perform the procedures below after the unit has been installed in a new workplace location. These prepare the incubator for normal use.
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OPERATION Continued from the previous page. Run the unit for at least 12 hours (for example, overnight) with chamber doors closed prior to: Setting the Over Temperature Limit (next step). • Loading samples. • This helps ensure a stable temperature environment. Perform the Set the Over Temperature Limit.
OPERATION UMIDIFY THE NCUBATOR The humidification pan must be filled with water and placed on the floor of the incubation chamber in order for the incubator to achieve its stated temperature uniformity specification. Humidifying the chamber helps slow the drying of samples in open, “breathable” containers. Always place and secure the copper token in the pan to slow the growth of microbiological •...
OPERATION ET THE EMPERATURE ETPOINT Perform the steps below to adjust the setpoint to your process or application temperature. Set the Over Temperature Limit control to its maximum setting, if not already set to max. This prevents the heating cutoff system •...
OPERATION UTING THE UDIBLE EMPERATURE LARM Audible and visual deviation alarms activate if the incubation chamber temperature deviates by 1°C above or below the temperature setpoint. The low deviation alarm has a delay of 15 minutes to prevent the low alarm from sounding each time the doors are opened, causing a short-lived drop in temperature.
OPERATION TART A LOW OF Gas Cylinder(s) Open the cylinder valve to supply CO to the regulator. Set cylinder regulator flow setting. Single source: Set the regulator to deliver 15 – 20 Pounds per Square Inch (psi) to • the incubator CO in Port.
OPERATION ET THE ETPOINT The incubator comes from the factory with the CO setpoint set to Off. Set the setpoint percentage to the gas concentration required by your study or production protocol. Navigate to the CO Setpoint Adjustment mode Briefly push and release either the Up or Down arrow buttons to activate the CO setpoint adjustment mode.
OPERATION UTING THE UDIBLE LARM Audible and visual deviation alarms activate if the incubation chamber CO concentration deviates by 1% above or below the CO setpoint. The low deviation audible alarm has a delay of 15 minutes to prevent the low alarm from sounding each time the doors are opened, causing a short-lived drop in CO concentration.
OPERATION Note: Test the OTL system at least once per year to verify its functionality. Failure to set the OTL voids the manufacturing defect warranty if over temperature damage occurs. (OTL) ET THE EMPERATURE IMIT This procedure sets the mechanical heating cutoff to approximately 1°C above the current chamber temperature.
OPERATION OADING AMPLES The manufacturer strongly recommends waiting at least 12 hours after putting the incubator in operation before loading samples in the chamber. This safeguards against temperature instability. • Samples should be placed at least 1 inch (25 mm) away from the chamber walls. •...
OPERATION UTPUT APABILITIES USB Outputs The incubator generates data outputs describing the chamber temperature and CO concentration percentage as a digital log line, once per minute. These outputs are transmitted through a USB port located on the right side of the unit using RS232 serial protocol. Required Software Viewing and logging this output requires a terminal emulator program such as the open source (BSD license) emulator, Tera Term.
OPERATION ONDENSATION AND THE OINT Relative humidity inside the incubator chamber should never be allowed to exceed 95%. Exceeding this threshold will likely result in condensation in the chamber and subsequent leakage from the incubator. This may cause corrosion damage if allowed to continue for any significant length of time.
USER MAINTENANCE Warning: Disconnect the unit from its power supply prior to performing maintenance or services. Avertissement: Débranchez cet appareil de son alimentation électrique avant d'effectuer la maintenance ou les services. LEANING AND ISINFECTING If a hazardous material or substance has spilled in the unit chamber, immediately initiate your site Hazardous Material Spill Containment protocol.
MAINTENANCE Disinfecting For maximum effectiveness, disinfection procedures are typically performed after cleaning. Keep the following points in mind when disinfecting the unit. Turn off and disconnect the unit to safeguard against electrical hazards. • Disinfect the unit chamber using commercially available disinfectants that are non-corrosive, •...
MAINTENANCE HEPA F INES AND ILTERS For the Optional Filtered Gas Tubing Kit The manufacturer recommends replacing the in-line gas HEPA filters once per year or “IN” when a filter is noticeably discolored. HEPA filters are directional and must be installed facing in the correct direction. •...
MAINTENANCE HEPA F EPLACE THE HAMBER ILTER Note: Always turn off and unplug the incubator before carrying out this procedure. Replace the filter at least once per year, or when the filter is noticeably discolored. Exercise caution when removing the ceiling air duct. A plastic blower fan and the fragile head of the temperature and CO sensors are located just above the duct and just to the right of the duct.
MAINTENANCE ALIBRATE THE EMPERATURE ISPLAY Note: This procedure requires a temperature reference device. Please see the Reference Sensor Device entry on page 10 for the device requirements. Temperature calibrations match the temperature display to the actual air temperature inside the incubation chamber.
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MAINTENANCE 6. The unit temperature must be stable in order to perform an accurate calibration. The incubator must run for at least 12 hours prior to conducting a calibration. • The temperature is considered stabilized when the incubator has operated with the door •...
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MAINTENANCE Temperature Calibration Continued Place the display in temperature calibration mode. a. Press and hold both the Up and Down temperature arrow buttons simultaneously for approximately 5 seconds. b. Release the buttons when the temperature display shows the letters “C O”. The display will begin flashing the current temperature display value.
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MAINTENANCE Temperature Calibration Continued Compare the reference device reading with the chamber Reference Device temperature display. If the reference device and the chamber temperature • display readings are the same or the difference falls within the range of your protocol, the incubator is now calibrated for temperature.
MAINTENANCE ALIBRATE THE ISPLAY Note: This procedure requires a gas reference device. Please see the Reference Sensor Devices entry on page 10 for the device requirements. Gas calibrations are performed to match the incubator CO display to the actual CO concentration inside the incubation chamber.
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MAINTENANCE 5. The CO concentration in the incubation chamber must be stable in order to perform an accurate calibration. The unit must be thermally stable, having operated at your application temperature for at • least 12 hours prior to starting the gas calibration. After turning on a flow of CO to the chamber and setting the CO setpoint, allow the...
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MAINTENANCE Calibration Continued Place the incubator CO display in its calibration mode. a. Press and hold both the Up and Down CO arrow buttons simultaneously for approximately 5 seconds. b. Release the buttons when the CO display shows the letters “C O”. The display will then begin flashing the current CO display value.
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MAINTENANCE Calibration Continued Compare the reference device reading with the incubator CO display again. Reference Device If the reference device and the incubator display readings • are the same or the difference falls within the range of your protocol, the incubator is now calibrated for CO -OR- ...
MAINTENANCE NODE AND ATER UALITY A replaceable metal anode comes installed in a threaded port near the water-jacket fill port on the back of the unit. The anode dissolves when in contact with the mineral salts and dissolved gases found in tap water. This helps to protect the liner of the water jacket from corrosion well as well as scaling and other mineral deposits.
UNIT SPECIFICATIONS The SCO5W-2 Incubator is a 220 – 240 volt unit. Please refer to the incubator data plate for individual electrical specifications. Technical data specified applies to units with standard equipment at an ambient temperature of 25°C and at nominal voltage. The temperatures specified are determined in accordance to factory standard following DIN 12880 respecting the recommended wall clearances of 10% of the height, width, and depth of the inner chamber.
SPECIFICATIONS HELVES Shelves APACITY Cubic Feet Liters 144.0 Range Accuracy Recovery Time 0 – 20% ± 0.1% Less than 5 minutes EMPERATURE Range Uniformity Stability ± 0.2ºC at 37ºC ± 0.1°C @ 37°C Ambient +5ºC to 60ºC OWER AC Voltage Amperage Frequency 220 –...
PARTS LIST Part Part Number Part Part Number Fuse T6.3A 250V Anode, Water Jacket 5x20mm (Requires 2) 0260500 3300515 Access Port Stopper Gas Line HEPA Filter 7750506 2800525 Chamber HEPA FILTER Humidification Pan 995-00015 2800517 Chamber HEPA Filter Cap Leveling Foot 2700512 6500506 Power Cord 250-volt,...
Accessories and replacement parts can be ordered online at parts.sheldonmfg.com. If the required item is not listed online, or if you require assistance in determining which part or accessory you need contact SHEL LAB by emailing parts@sheldonmfg.com or by calling 1-800-322- 4897 ext. 3 or (503) 640-3000 ext. 3.
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