Phcbi MCO-5M Series Operating Instructions Manual

Phcbi MCO-5M Series Operating Instructions Manual

O2/co2 incubator
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Operating Instructions
O
/CO
Incubator
2
2
MCO-5M 
 
Series
Please read the operating instructions carefully before using this product, and keep the operating
instructions for future use.
See page 52 for model number.
 

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Summary of Contents for Phcbi MCO-5M Series

  • Page 1 Operating Instructions Incubator MCO-5M    Series Please read the operating instructions carefully before using this product, and keep the operating instructions for future use. See page 52 for model number.  ...
  • Page 3: Table Of Contents

    CONTENTS INTRODUCTION ....................2 PRECAUTIONS FOR SAFE OPERATION ............3 LABELS ON THE INCUBATOR ................8 ENVIRONMENTAL CONDITIONS ..............8 INCUBATOR COMPONENTS ................9 Control panel and keypad ................11 Remote alarm terminals ................13 INSTALLATION SITE ..................14 INSTALLATION ....................15 Connecting a CO gas cylinder ..............
  • Page 4: Introduction

    INTRODUCTION ■ Read the operating instructions carefully before using the Product and follow the instructions for safety operation. ■ PHC Corporation disavows any responsibility for safety if the Product is used for other than the intended use or used with any procedures other than those given in the operating instructions. ■...
  • Page 5: Precautions For Safe Operation

    PRECAUTIONS FOR SAFE OPERATION It is imperative that the user complies with the operating instructions as it contains important safety advice. Items and procedures are described so that you can use this unit correctly and safely. If the precautions advised are followed, this will prevent possible injury to the user and any other person.
  • Page 6 PRECAUTIONS FOR SAFE OPERATION USA Only (Model with a lamp): This product has a lamp that contains mercury. Disposal may be regulated in your community due to environmental considerations. For disposal or information, please visit PHC website: https://www.phchd.com. Contains mercury / Contenu avec mercure For more information on safe handling Pour plus de renseignements sur les procedures, the measures to be taken in...
  • Page 7 WARNING Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to rain water. Only qualified engineers or service personnel should install the unit. The installation by unqualified personnel may cause electric shock or fire. Install the unit on a sturdy floor and take an adequate precaution to prevent the unit from turning over.
  • Page 8 PRECAUTIONS FOR SAFE OPERATION WARNING Ensure you do not inhale or consume medication or aerosols from around the unit at the time of maintenance. These may be harmful to your health. Never splash water directly onto the unit as this may cause electric shock or short circuit. Never put containers with liquid on the unit as this may cause electric shock or short circuit when the liquid is spilled.
  • Page 9 CAUTION This unit must be plugged into a dedicated circuit protected by branch circuit breaker. Use a dedicated power source as indicated on the rating label attached to the unit. A multiple-tap may cause fire resulting from abnormal heating. Never store corrosive substances such as acid or alkali in this unit if the container cannot be sealed.
  • Page 10: Labels On The Incubator

    LABELS ON THE INCUBATOR Warning and caution labels are attached to the incubator. The following table describes the labels. This label is attached to covers that access high-voltage electrical components to prevent electric shock. Only a qualified engineer or service personnel should be allowed to open these covers.
  • Page 11: Incubator Components

    INCUBATOR COMPONENTS 7 (inside) When MCO-19UVS is installed 13 ,15 ,16(inside) Changeabl Switch cover Connecting port for N Connecting port for CO When MCO-19UVS is installed Rear right side Rear left side...
  • Page 12 INCUBATOR COMPONENTS 1. Outer door: Sticks to frame with magnetic seal. Door heater is installed in the door panel. The door opening is reversible. Contact our sales representative or agent to change the door hinge from left to right or vice versa. 2.
  • Page 13: Control Panel And Keypad

    Control panel and keypad 1. Digital temperature indicator (TEMPERATURE C): Normally, this indicator shows the chamber temperature. In the setting mode, it shows the set value of the chamber temperature. If the self diagnostic function detects any abnormality, an error code will be displayed. 2.
  • Page 14 INCUBATOR COMPONENTS 12. Set key (SET): Pressing this key to enter the setting mode, and the digits to be set will blink. 13. Calibration key (CAL): By pressing this key for approximately 5 seconds, the unit enters calibration function mode. See page 39 for the details. Also, used to change the UV lamp ON period. See page 34 for the details.
  • Page 15: Remote Alarm Terminals

    Remote alarm terminals The remote alarm terminal is located at the rear right side. Remote alarm terminal The remote alarm terminal is a contact output. Normal : OPEN Abnormal , blackout : CLOSE Contact capacity : DC 30 V, 2 A Note: •...
  • Page 16: Installation Site

    INSTALLATION SITE For correct operation of the incubator, install it in a location with the following conditions. WARNING When using CO gas for control, make sure that there is adequate ventilation. Using CO gas in a small room without adequate ventilation may cause gas poisoning or oxygen deprivation. In addition, when opening the incubator doors, do not directly inhale the air in the chamber.
  • Page 17: Installation

    INSTALLATION 1. Remove the packing tape and clean up. Remove all the tape that is securing the doors and inner attachments. Open the doors for ventilation. If the outer panels are dirty, dampen a cloth with a diluted neutral detergent and wipe them. (Using an undiluted solution may damage the plastic.
  • Page 18: Connecting A Co Gas Cylinder

    INSTALLATION Connecting a CO gas cylinder WARNING When connecting a gas cylinder to the incubator, confirm the gas type. Confirm that the connections are secure and that no gas will escape. Be sure to use the specified pressure. Using an incorrect gas or pressure may result in explosion or fire, or in gas poisoning or oxygen deprivation due to escaping gas.
  • Page 19: Connecting A N (Or O ) Gas Cylinder

    Connecting a N (or O ) gas cylinder WARNING Check the gas type and ensure that it is fit for the purpose. Make sure that all pipes are connected correctly and are not liable to become disconnected. Ensure that the gas pressure is set at the specified value.
  • Page 20: Connecting A Gas Injection Nozzle

    INSTALLATION Connecting a gas injection nozzle In the case of control of O density in the chamber, connect the gas injection nozzle to the gas injection port by using the gas injection nozzle tube enclosed (inner diameter; 5 mm, outer diameter; 9 mm, length; 300 mm).
  • Page 21: Preventing Contamination

    PREVENTING CONTAMINATION To prevent contamination of the chamber, select a suitable installation site.  Avoid locations with high temperatures or humidity. Avoid locations with high temperatures or humidity, because of a greater presence of microorganisms in the air.  Avoid locations with passers-by or drafts. Avoid locations near doors, air conditioners, fans, etc., where passers-by or drafts can facilitate the entry of microorganisms into the chamber.
  • Page 22: Precautions For Cultures

    PRECAUTIONS FOR CULTURES  Leave space between culture containers. Always leave space for ventilation between culture containers (Petri dishes, flasks, etc.). Inadequate spacing may result in uneven temperature distribution and CO gas density.  Do not place harmful materials in the chamber. Never place samples that release acidic, alkali, or corrosive gas in the chamber.
  • Page 23: Initial Cleaning Method

    INITIAL CLEANING METHOD Before using the incubator for the first time, clean dirt (tape residue, smear, etc.) from the chamber and the inner attachments thoroughly. To keep the chamber clean is essential to get the proper performance out of the incubator. Use the following steps to clean the incubator properly.
  • Page 24: Removing Inner Attachments

    REMOVING INNER ATTACHMENTS CAUTION Wear rubber gloves when performing maintenance on the chamber. Failure to wear gloves may result in cuts or abrasions from sharp edges or corners. Be careful not to damage the water level sensor or the UV lamp in the rear duct. (When an optional UV system set MCO-19UVS is installed.) Do not use detergents or antiseptic solutions with acid, alkali, or chlorine.
  • Page 25 8. Lift the rear duct and remove it from the pins at the rear side. (Fig. 7) 9. Remove the fan by pulling out the central spring and then by pulling out the fan. (Fig. 8) 10. Remove the silicon caps of the access port, one each from interior (Fig. 9) and exterior (Fig. 10). 1.
  • Page 26: Installing Inner Attachments

    INSTALLING INNER ATTACHMENTS Use the following steps to install the inner attachments properly. 1. To reinstall all the attachments, perform the procedure in reverse order from step 10 on page 23. 2. When installing the fan, insert it to the motor shaft securely. Lightly turn the fan manually to make sure that it does not strike the rear panel.
  • Page 27: Filling The Humidifying Pan

    FILLING THE HUMIDIFYING PAN Use the following procedure to fill the humidifying pan or to replace the water. 1. Lift the front side of the humidifying pan cover. (Fig. 1) 2. Pull the humidifying pan forward. (Fig. 2) 3. Dispose of the remaining water in the humidifying pan and clean the humidifying pan with a diluted detergent.
  • Page 28: Water Level Sensor

    WATER LEVEL SENSOR This incubator is equipped with a water level sensor. The water level sensor is set automatically when the humidifying pan is installed. Take care not to damage the water level sensor when removing or installing the humidifying pan. When the humidifying pan is removed (side view) Water level sensor Humidifying pan...
  • Page 29: Routine Maintenance

    ROUTINE MAINTENANCE To use this unit in a clean condition, clean the chamber and the inner attachments at least once a month. 1. Remove the inner attachments by the procedures shown on page 22. 2. Clean the chamber and the inner attachments by the procedures shown on page 21. 3.
  • Page 30: Operation Of Keys On The Control Panel

    OPERATION OF KEYS ON THE CONTROL PANEL Table below shows the basic procedure for setting the chamber temperature, CO and O density. The high limit alarm temperature setting is also shown in the table. Perform key operations in the sequence indicated in the table.
  • Page 31: Setting Of Alarm Resume Time

    SETTING OF ALARM RESUME TIME The alarm buzzer is silenced by pressing the alarm buzzer stop key (BUZZER) on the control panel during alarm condition. The buzzer will be activated again after certain suspension if the alarm condition is continued. The suspension time can be set by following the procedure shown in the table below.
  • Page 32: Key Lock Function

    KEY LOCK FUNCTION This unit is provided with a key lock function. When the key lock is ON, change of temperature, CO density setting through the key pad is not available. Note: The key lock is set in OFF mode at the factory. Display Mode Function...
  • Page 33: Automatic Gas Cylinder Changeover

    AUTOMATIC GAS CYLINDER CHANGEOVER This incubator is provided with an automatic N (or O ) gas cylinder changeover system, which switches the gas (N or O ) cylinder when one gas cylinder becomes empty. Also, a gas auto changer (MCO-5GC) is available as an optional accessory. This kit switches the gas supply line when one CO gas cylinder becomes empty.
  • Page 34 AUTOMATIC GAS CYLINDER CHANGEOVER 3. Following shows the procedure to changeover the gas supply line (N gas cylinder and CO cylinder) manually. (Example: Change of CO gas cylinder from B to A, Change of N gas cylinder from A to B) Gas supply line Operation Indication after operation...
  • Page 35: Uv Lamp

    UV LAMP The clauses below are applicable when an optional UV system set MCO-19UVS is installed. A UV lamp is located inside the rear duct to sterilize the water in the humidifying pan and air circulating in the chamber. Following shows precautions and instructions about the UV lamp. •...
  • Page 36: Change Of Setting For Uv Lamp On Period

    UV LAMP Change of setting for UV lamp ON period The clauses below are applicable when an optional UV system set MCO-19UVS is installed. Follow the procedure below when changing the setting for UV lamp ON period. Basic operation sequence (Example: change of UV lamp ON period from 5 minutes to 3 minutes) Description of operation Key operated Indication after operation...
  • Page 37: Precautions When Using The Uv Lamp

    Precautions when using the UV lamp The cautions below are applicable when an optional UV system set MCO-19UVS is installed. ● Always use humidifying pan and humidifying pan cover The humidifying pan and humidifying pan cover prevent the UV light from escaping. Make sure they are installed even if you do not need humidity.
  • Page 38: Lighting The Uv Lamp For 24 Hours

    UV LAMP Lighting the UV lamp for 24 hours Follow the procedure below when lighting UV lamp for 24 hours. Before sterilization by UV lamp, remove all attachments in the chamber (tray, side support, rear duct, fan, humidifying pan, humidifying pan cover, gas injection nozzle, gas injection nozzle tube) and clean the interior, inner door and UV lamp thoroughly.
  • Page 39: Alarms, Safety And Self-Diagnosis

    ALARMS, SAFETY AND SELF-DIAGNOSIS This unit has the alarms and safety functions shown in table below, and also self diagnostic functions. Alarms and safety functions Alarm & Safety Situation Indication Buzzer Safety operation Over heat lamp lights. If the chamber temperature exceeds High limit E12 or E16 and chamber Heater OFF...
  • Page 40 ALARMS, SAFETY AND SELF-DIAGNOSIS Alarms and safety functions Alarm & Safety Situation Indication Buzzer Safety operation E09 is displayed alternately If the ambient temperature sensor is with the temperature on the disconnected. Ambient digital temperature indicator. temperature sensor Intermittent tone Remote alarm E10 is displayed alternately abnormality...
  • Page 41: Calibration

    CALIBRATION Temperature calibration 1. Press the calibration key (CAL) for approximately 5 seconds. 2. The left digit of the digital temperature indicator blinks, and the other indicators go out. 3. Set the present correct temperature with the digit shift key ( ) and numerical value shift key ( then press the enter key (ENT).
  • Page 42: Calibration

    CALIBRATION calibration [Zero setting] Zero setting should be done when CO and O has not been injected yet and the stable condition (about 8 hours) of temperature/humidity inside the unit has been attained. 1. Press the calibration key (CAL) for 5 seconds. 2.
  • Page 43: Co 2 Calibration

    calibration [Zero setting] Zero setting should be done when CO and O has not been injected yet and the stable condition (about 8 hours) of temperature/humidity inside the unit has been attained. 1. Press the calibration key (CAL) for 5 seconds. 2.
  • Page 44: Troubleshooting

    TROUBLESHOOTING If the unit malfunctions, check out the following before calling for service. Malfunction Check/Remedy • The unit is not plugged correctly into a power outlet. • The circuit breaker at the power source is active or a power failure The unit does not operate has occurred.
  • Page 45 Malfunction Check/Remedy • The environment around the unit is not normal. The source of the If normal cultivation cannot contaminated gas is nearby. be done and chamber gas • The unit is installed in an enclosed space. density is suspect •...
  • Page 46 DISPOSING OF THE O INCUBATOR When disposing of the O incubator, contact our sales representative or agent. WARNING The O incubator must be dismantled and disposed of by qualified personnel only. If the incubator is left where outsiders enter, it may result unexpected accident (for example, children to become locked inside).
  • Page 47 Note: This symbol mark and recycle system apply only to EU countries. They do not applied to the countries in other areas of the world. (English) Information on Disposal for Users of Waste Electrical & Electronic Equipment (private households) This symbol on the products and/or accompanying documents means that used electrical and electronic products should not be mixed with general household waste.
  • Page 48: Disposing Of The O /Co Incubator

    DISPOSING OF THE O INCUBATOR (French) Informations relatives à l’évacuation des déchets, destinées aux utilisateurs d’appareils électriques et électroniques (appareils ménagers domestiques) Lorsque ce symbole figure sur les produits et/ou les documents qui les accompagnent, cela signifie que les appareils électriques et électroniques ne doivent pas être jetés avec les ordures ménagères.
  • Page 49 (Portuguese) Informações sobre a eliminação de resíduos para utilizadores de equipamentos eléctricos e electrónicos (utilizadores particulares) Este símbolo nos produtos e/ou documentos anexos significa que os produtos eléctricos e electrónicos usados não devem ser misturados com os resíduos urbanos indiferenciados. Para efectuar um tratamento, recuperação e reciclagem correctos, leve estes produtos para pontos de recolha próprios para o efeito, onde serão aceites gratuitamente.
  • Page 50 DISPOSING OF THE O INCUBATOR (Dutch) Informatie over het weggooien van elektrische en elektronische apparatuur (particulieren) Dit symbool betekent in Europa dat gebruikte elektrische en elektronische producten niet bij het normale huishoudelijke afval mogen. Lever deze producten in bij de aangewezen inzamelingspunten, waar ze gratis worden geaccepteerd en op de juiste manier worden verwerkt, teruggewonnen en hergebruikt.
  • Page 51: Stacking Incubators

    STACKING INCUBATORS Use the following procedure to stack incubators. This work is potentially dangerous, so contact our sales representative or agent. CAUTION  Select a floor that is strong enough to support the stacked incubators.  Never stack 4 or more incubators. Doing so is dangerous. (When stacking 2 incubators) 1.
  • Page 52 STACKING INCUBATORS Front panel Stacking plate B Stacking plate A Protective sticker Fig.A <View from quarter rear side>...
  • Page 53: Specifications

    SPECIFICATIONS Product name Incubator MCO-5M External dimensions W480 mm x D548 mm x H575 mm (W18.9 inch x D21.6 inch x H22.6 inch) Internal dimensions W350 mm x D378 mm x H375 mm (W13.8 inch x D14.9 inch x H14.8 inch) Interior volume 49 L (1.73 cu.ft.) Exterior...
  • Page 54: Performance

    SPECIFICATIONS Product name Incubator MCO-5M Accessories 1 power supply removable cord* , 3 trays, 3 gas tube, 1 humidifying pan 1 gas injection nozzle, 1 gas injection nozzle tube 1 pair of stacking plates A and B, 4 protective stickers, 6 tube bands Weight 50 kg Optional accessories...
  • Page 55: Safety Check Sheet

    CAUTION Please fill in this form before servicing. Hand over this form to the service engineer to keep for his and your safety. Safety check sheet 1. Unit contents □ □ Risk of infection: □ □ Risk of toxicity: □ □...
  • Page 56 1-1-1 Sakada, Oizumi-machi, Ora-gun, Gunma 370-0596, Japan Printed in Japan LDCL041900-0 © PHC Corporation 2018 S0418-0...

This manual is also suitable for:

Mco-5m-ptMco-5m-paMco-5m-pkMco-5m-pe

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