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Okolab UNO-COMBINED-CONTROLLER Manual

Сo2 microscope electric top stage incubation system
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UNO-COMBINED-CONTROLLER
CO
Microscope Electric Top Stage Incubation System
2
Manual
Vers. 03.16
1

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Summary of Contents for Okolab UNO-COMBINED-CONTROLLER

  • Page 1 UNO-COMBINED-CONTROLLER Microscope Electric Top Stage Incubation System Manual Vers. 03.16...
  • Page 2 This page was left blank...

  • Page 3: Table Of Contents

    UNO-COMBINED-CONTROLLER. OVERVIEW ..................13 Temperature Regulation ....................13 Regulation ....................... 13 Humidity Regulation......................14 UNO-COMBINED-CONTROLLER. INSTALLATION AND USER GUIDE..........15 Digital Temperature Controller connection ports................ 15 Tubing Connections......................16 Pressure Gauge set up and use ................... 19 Assembling Instruction of the Bubbling Column ................. 21 Cables connection.
  • Page 4 Screen Settings Submenu ....................46 Product Information Submenu ..................47 Stop Mode ........................47 UNO-TS software Active Message ..................48 OBJECTIVE HEATER MANUAL CALIBRATION .................. 48 FLOW METER REGULATION ......................... 49 MAINTENANCE ............................51 SUPPORT ..............................52 Web Conference For Assistance And Training ................52 Troubleshooting ......................

  • Page 5: Revision Table

    Revision table Revision Number Additions or changes Date Editor Edited November 2014 Amelia Liccardo Silvia Foppiano Review March 2015 Amelia Liccardo Minor Review June 2016 Francesco Marino...

  • Page 6: Preface

    An Objective Heater is REQUIRED when using oil immersion objectives. Additionally, UNO-COMBINED-CONTROLLER can be equipped with an optional air pump: OKO- AP, which is a plug and play solution for Air inlet and a convenient alternative to 100% Air tanks/compressed Air lines.

  • Page 7: Symbols Descriptions

    Safety Notes Before operating the equipment please read carefully the instructions and the safety notes. If you have any questions, please contact Okolab. The equipment must only be used as intended and as described in this Manual. Technically qualified personnel should only operate equipment.
  • Page 8 the instrument. Not following these instructions can result in damage or breakdown of the device and its accessories. The products labels can be found on the rear panel of the Main Unit. Do not exceed voltage indicated in this manual and on the product label. Avoid excessive induction noise, static electricity, magnetic fields.

  • Page 9: Ac Section Wiring Diagram

    We reserve the right to make technical variations. IN NO EVENT SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL DAMAGES OF ANY NATURE, FINANCIAL OR OTHER LOSSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE OF ANY PRODUCT.
  • Page 10 2.5A 2.5A Power Connector OM-BZ03105 1mmq BROWN Power Supply Power Supply TDK-Lambda TDK-Lambda ZWS150-24 ZWS5-5 85 → 265V c.a. 85 → 265V c.a. 24V c.c. 5V c.c. 6.5 A POSITIVE CLAMPS 24 V DC 24V-DC 24V-DC 24V-DC 24V-DC 24V-DC BLUE 5V-DC 5V-DC NEGATIVE...

  • Page 11: Uno-Combined-Controller. Description

    Air port labeled “Air In” on rear panel of the Control Unit. In this working modality, Okolab recommends setting a flow rate of 0.3 L/min using the floating ball flow meter on the front Panel of the Control Unit.

  • Page 12: Equipment And Connections

    Equipment And Connections Equipment Supplied UNO includes: 1. Control-Unit 2. Humidifying Module Heater 3. Glass Bottle - Humidifying Module (attention: FRAGILE) with TUBE E (140 mm silicon tube of 6 mm OD with Filter). 4. Pressure gauge with filter, regulator and assembly stirrup for CO , degree of filtration 20μm and condensate drain.

  • Page 13: Equipment Required (Not Supplied)

    3. Alternatively to 100% CO2 + air UNO can be operated with a pre-mixed 5% CO supply. UNO-COMBINED-CONTROLLER. Overview UNO-COMBINED-CONTROLLER is at the same time a temperature and a gas controller. It is designed to maintain all the required thermal conditions for live cell imaging and is ideal for time-lapse imaging applications.

  • Page 14: Humidity Regulation

    Humidity Regulation Humidifying and pre-heating modules prevent medium evaporation and avoid water condensation on glass and plastic surfaces.

  • Page 15: Uno-Combined-Controller. Installation And User Guide

    UNO-COMBINED-CONTROLLER. Installation And User Guide The following paragraphs illustrate how to install and use UNO-COMBINED-CONTROLLER. The following instructions will guide you through a quick installation. For safe operation of the unit please read carefully all the instructions and safety notes.

  • Page 16: Tubing Connections

    7. Heater 1: Chamber Base connector 8. Fuse 1: 1.6 A Fast Fuse 9. Heater 2: Chamber Lid connector 10. Fuse 2: 1.6 A Fast Fuse. 11. H.M.: Humidifying module-heating connector. 12. Fuse 3: 1.6 A Fast Fuse. 13. Obj. Heater: Objective Heater Connector. 14.
  • Page 17 Swift-Fit Connector for rigid plastic tube with 6mm O.D. and connect TUBE D (supplied, see paragraph 7.1). If you need further assistance please contact Okolab. Secure the Pressure Gauge for CO with regulator to the wall by using the supplied assembling stirrup (supplied accessories).
  • Page 18 the controller and labeled “CO2 IN”. Push the tube all the way in into the Swift-Fit connectors. To remove the tube, pull the tube out while pushing the black ring on the Swift-Fit connector. Follow the arrow on the Pressure Gauge for the correct gas flow direction as illustrated in Figure 7.

  • Page 19: Pressure Gauge Set Up And Use

    Swift-Fit connector on the rear panel of the Control Unit to the Inlet barb connector on the Humidifier bottle. Replace that filter every 6 months. Contact Okolab for further information. Avoid pinching and kinking of all tubing as it will hinder gas flow and may cause condensation.
  • Page 20 Figure 8. How to close the purging valve of the Pressure Gauge. To close the gauge, pull the knob up and rotate it counterclockwise. Push the knob down to lock it. Open valve on gas supply tank. Adjust Pressure Gauge by pulling the knob up and rotating it clockwise until the pressure reaches the desired value (See Figure 9).

  • Page 21: Assembling Instruction Of The Bubbling Column

    Assembling Instruction of the Bubbling Column Outlet tubing Connector Inlet tubing Connector Head Screw Cap with o-ring Tubing Connector O-ring TUBE E Filter Figure 10. Assembling instruction of the Bubbling Column and its parts 1. Unscrew the red cap with O-ring and the head of the bubbling column from the bottle, without removing the head from the plug.
  • Page 22 Control Unit (see Figure 11). Place this thermocouple in a Reference Well filled with water. Make sure that the end of the Fine Gauge Thermocouple is fully immersed in liquid. Serial Communication Cable UNO-COMBINED-CONTROLLER To PC Humidifying Module Heater...
  • Page 23 Figure 12. Objective Band Heater Selection Chart. If your system is also equipped with the Objective Heater, (OBJ-COLLAR ####, optional) its objective collar should be installed before connecting the heater to rear panel of the Temperature Control Unit. Follow the steps shown in Figure 13 Remove the microscope objective and place it on the desk Place the orange colored Heating Band around the microscope Objective, cover it with the Velcro tape and fix the Velcro Tape as illustrated in Figure 13...

  • Page 24: Incubation Chamber

    Figure 13. Objective Heater (OBJ-COLLAR ####) Assembly 8. Connect the power cord to the Control Unit. Once the system has been initialized, the UNO-COMBINED-CONTROLLER display will show its homepage. Tip ► Wait until the system has reached the steady state before loading your samples.
  • Page 25 Tip ► The only value that you have to insert, on the push button display, is the base offset, while the lid and HM offsets are automatically calculated when you insert the base offset. If your sample adapter is not present in Table 1, you can use the Custom configuration and calibrate the chamber in your lab (See paragraph 13.5.2) Tip ►...
  • Page 26 Incubation Chamber Sample Adapter Base Offset 1x35-M; 2x35-M, -3.3 2x35-PRIOR-NZ500 2x60-M; 2x60-PRIOR-NZ500 -1.6 4x35-M; 4x35-PRIOR-NZ500 -1.6 H301-K-FRAME 2xGS-M; 2xGS-PRIOR-NZ500 -4.2 H301-NIKON-TI-S-ER 6MW; 6MW-PRIOR-NZ500 -1.9 H301-OLYMPUS-IX3-SVR 12 MW; 12 MW-PRIOR-NZ500 -2.3 H301-PRIOR-H117 24 MW; 24 MW-NUNC; H301-NIKON-NZ100/200/500-N 24 MW-PRIOR-NZ500; -0.6 H301-MCL-Z100/500 24 MW-NUNC -PRIOR-NZ500 H301-LUDL 96A602 GS35-M;...

  • Page 27: Fine Gauge Thermocouple (Free Sensor-Optional)

    Fine gauge thermocouple (Free Sensor-optional) Connect them togheter Free Sensor Fine gauge thermocouple Figure 14. Free Sensor with male to male extending cable. The incubating system can be equipped with an external Free Sensor (Fine Gauge Thermocouple- Optional added to the system with the purchase of UNO-TS software), which allows measuring the temperature inside a reference well.

  • Page 28: Control Unit Front Panel

    10 Control Unit Front Panel Figure 15. Front Panel of the Control Unit The front panel of the Control Unit consists of a display with four function keys and a gas mixer. After the unit has been switched on and the warm up screen disappears the display will show the “Main Page”.

  • Page 29: Temperature Control Mechanism In Standard Configuration

    Enter the Offset Submenu (see paragraph 13.5.1). Reading Offsets were evaluated with a careful calibration performed by Okolab at ambient temperature of 23+/-1°C. This guarantees that the sample is maintained at the desired set point temperature without the need of further calibrations.
  • Page 30 Once you have selected Standard mode, you can change the set point according to your needs: From the ‘Main Page’, press ‘Enter’ to change the Set point Temperature. Press ‘up’ or ‘down’ arrows button to increase or to reduce the Set Point Temperature while it is flashing.

  • Page 31: Temperature Control Mechanism In Custom Configuration At 37.0°C

    SENSOR LID-# to close the dish (see the “Tip” below). Tip ► It is recommended using an Okolab SENSOR LID (available for purchase) to secure the Fine Gauge Thermocouple in place in the Reference Well during calibration (See Figure 17).
  • Page 32 SENSOR LID CODES SENSOR LID-35 Temperature sensor lid. To be used in specimen feedback in a 35 mm Petri Dish Temperature sensor lid. To be used in specimen feedback in a 60 mm Petri Dish SENSOR LID-60 Temperature sensor lid. To be used in specimen feedback in chamber slides and SENSOR LID-GS chambered cover glass Table 2.
  • Page 33 Press ‘up’ or ‘down’ arrows keys to increase or to reduce the Set Point Temperature while it is flashing. Press ‘Enter’ to confirm the new Set Point temperature. Go to the Sub Menu ‘Offset’ and change the actual Reading Offset of BASE, LID and HM temperatures, in order to have the sample at the set point temperature, as described below.

  • Page 34: Unit Operation Through Push Button Interface

    Desired well Base H.M. Ambient temperature temperature Offset Offset Offset [°C] [°C] [°C] [°C] [°C] 32.0 -1.1 -2.2 -1.7 33.0 -1.1 -2.4 -2.0 34.0 -1.2 -2.7 -2.2 35.0 -1.3 -2.9 -2.5 23.0 ± 1.0 36.0 -1.4 -3.2 -2.7 (Suggested ambient temperature) 37.0 (Default) -1.5 -3.5...

  • Page 35: Button Functions

    The controller has four Function Buttons: “Menu”, “up”, “down” and “Enter” buttons. Button functions ‘Menu’ button To access the main menu, press “Menu”. While a parameter is being modified, press “Menu” to escape without saving the parameter. Use “Menu” button to go up or to exit from a “Sub Menu”. ‘Up and down’...

  • Page 36: Main Page And Main Menu Navigation

    Main Page and Main Menu Navigation OKOlab 5 seconds Combined Controller Set Point 37.0°C Base 37.0 HM 37.0 Lid 37.0 OBH 37.0 Menu 1/3 à Offset Mode Offset â Objective Heater Menu 2/3 à Humidity Module Alarm Settings â Screen Settings Menu 3/3 à...

  • Page 37: Changing Set Points

    Changing Set Points Change the Set Point Confirm new Set Point Figure 20. Main Menu Display. Changing Set Points Description. ‘Main Page’ displays actual temperatures of Base, Lid, Objective Heater and Humidity Module and the Set Point value. Tip ► The Objective Heater and humidity module temperatures are shown on display only if they are active (See paragraphs 13.6 and 13.7) Press ‘Enter’...
  • Page 38 Figure 22. Offset Mode Selecting. Enter the Main Menu pressing the ‘Menu’ Key Move the cursor to ‘Offset Mode’ and press ‘Enter’. This will show the current base offset in Standard Working Mode. Tip ►The system is shipped from the factory already set according to the incubating chamber model and sample adapter you have ordered and in standard working mode.
  • Page 39 Once you have entered the Main Menu pressing the ‘Menu’ Key: Move the cursor to the item ‘Offset’ with the ‘Arrow’ Keys and press ‘Enter’. Here you can read the offset values. If you are using a CUSTOM PLATE ADAPTER, not included in the list of standard adapters or if the room temperature differs more than 2°C from 23°C (temperature of the factory calibration) follow this procedure: To change the ‘Reading Offset’...

  • Page 40: Custom Offset Mode

    2°C from 23°C (temperature of the factory calibration) or if you use a custom plate adapter, non-included in the list reported in Table 1, Okolab recommends this working mode recalibrating the chamber following the procedure reported in paragraph 12.

  • Page 41: Objective Heater Submenu

    Set Point 37.0°C Base 37.0 HM 37.0 Lid 37.0 OBH 37.0 Menu 1/3 à Offset Mode Offset â Objective Heater Menu 1/3 Offset Mode à Offset Objective Heater Insert the Offset à Base: -01.2 Enter to Lid: -03.2 Insert Offset Exit confirm Values...
  • Page 42 Heater Temperature is no longer displayed, as illustrated in Figure 28 Moreover, in the Objective Heater sub Menu the User can change the offset and select the option ‘Reset to factory’ to reset Objective Heater Settings to the default values. (See Figure 29) Set Point 37.0°C Base 37.0...
  • Page 43 Move the cursor to the item ‘Offset’ using the Arrow Keys. Press ‘Enter’ with the cursor on the item ‘Offset’ and then ‘Up/Down’ to change the offset value Press ‘Enter’ to Confirm Tip ► The range of the ramp rate values goes from -10°C to 10°C. The User can change the Ramp rate if needed.

  • Page 44: Humidity Module Submenu

    Humidity Module Submenu Set Point 37.0°C Base 37.0 HM 37.0 Lid 37.0 OBH 37.0 Menu 1/3 à Offset Mode Offset â Objective Heater Menu 2/3 à Humidity Module Alarm Settings â Screen Settings Humidity Module á à Enabled: Choose Change Confirm On/Off Exit...

  • Page 45: Alarm Settings Submenu

    Alarm Settings Submenu Menu 2/2 à Alarm Settings Screen Settings Product info Alarm Settings 1/2 à Alarm: á Move the cursor to the Buzzer: Select the value Parameter to be â to be changed Alarm Dev,°C: changesd Alarm Settings 2/2 á...
  • Page 46 Set Point 37.0°C Base Err_2 37.0 37.0 37.0 Figure 36. Temperature sensor fault. In Figure 39 above, ERR_2 means that there is no temperature reading form the base of the chamber. This happens if the sensor embedded into the base plate of the chamber is faulty or if its cable is disconnected from the outlet labeled T1.
  • Page 47 Product Information Submenu Figure 38. Product Info Menu Product Info Sub Menu enables the operator to display information such as Software Version (Ver), Serial number (S/N), Service date of the last Factory calibration and set up of the controller. From the main menu press the Menu key on the front panel. Move the cursor to ‘Product Info’...
  • Page 48 UNO-TS software Active Message Figure 40. Display when UNO-TS software is active. When UNO-TS software is active, Menu navigation is disabled 14 Objective Heater Manual Calibration Carefully read the instructions given in this paragraph and in paragraph 13.6 before starting the Objective Heater Manual Calibration. The goal of this procedure is to adjust the controlled Objective Heater temperature, manually changing its offset;...
  • Page 49 Gas flow rate values to set Percentage [Nl/min] Total 95.00 5.00 0.60 0.03 0.63 95.00 5.00 0.80 0.04 0.84 95.00 5.00 1.00 0.05 1.05 Table 4. Flow meter regulation. Nl [Normal/liter]. Tip ► If you need, ask Okolab for personalized Gas Percentage Tables.
  • Page 50 ‘Target Gas Percentage’ indicates the stream gas percentage that the operator wants during the experiment. ‘Gas flow rate values to set’, in Normal liter per minute [Nl/min], are the values of gas flow rate to set on the graduated scale (see Figure 41). ‘Total’...
  • Page 51 Never use thinners, benzene, solvents on or near the devices, since these could corrode their surfaces. To clean the Stage Incubator and the Humidifying Module, if it is present, you can use distilled water or alcohol Verify the status of all cables and if some cable is damaged, contact Okolab to receive assistance...
  • Page 52 17 Support Web Conference For Assistance And Training Together with your equipment you received a web cam and headset. You can request remote support over the web. Please follow these guidelines to facilitate setting up your web support: Webcam installation (instruction and Cdrom included) Updated Skype ®...
  • Page 53 Incorrect operation of the unit is often mistaken for malfunction. If you think that there is something wrong with a component, see the troubleshooting scheme below. If the problem persists even after troubleshooting as described below, please contact for Okolab support. Symptom...
  • Page 54 Technical Support Please, do not hesitate to contact Okolab should you need any further commercial information or technical support. Please, check Okolab web site www.oko-lab.com for news, events, new products and general FAQ. COMMERCIAL SUPPORT: lanzaro@oko-lab.com Phone +39 081 806 2624...
  • Page 55 18 Figures Figure 1 AC section wiring diagram ..................... 10 Figure 2. System Overview....................... 11 Figure 3. Control Unit Rear Panel Overview ................... 15 Figure 4. Complete gas lines scheme....................16 Figure 5. Complete gas lines scheme. Zoom sketches on the Inlet and Outlet connectors of the Humidifier and Zoom sketch on the Inlet connector of the Chamber are reported.
  • Page 56 Okolab S.r.l. accepts no responsibility for damage due to unsatisfactory packing. Upon examination of Okolab S.r.l., if the unit is found to be defective, it will be repaired or replaced at no charge. This warranty does not apply to defects resulting from any actions of the purchaser.

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