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Summary of Contents for Okolab H301-MINI
- Page 1 Okolab stage top incubator H301-MINI tutorial...
- Page 2 Heated & humidified 5%CO 1) Check the H O level in the bottle (if empty fill it with distilled H 2) Open the Air and CO regulators (looking to the screen the regulators are on the left wall at the end of the LSM700 row) Down = open Up = close...
- Page 3 Objective heater 1/2 If an immersion objective will be used, mount around it the objective heater. 1) Remove the holder 2) Select the objective that you will use...
- Page 4 Objective heater 2/2 3) Bring the objective heater from below the stage (heated orange band+green velcro tape) 4) Place the objective heater around the objective 5) Tighten the velcro Pay attention to do not entangle the cables if you are switching to other objectives.
- Page 5 Install the chamber 1/3 1) Install the multiwell plate holder 2) Place the chamber without the gas tube (disconnect the tube if needed)
- Page 6 Install the chamber 2/3 3) Open the chamber removing the glass lid (unscrew the two lateral screws) 4) Place the appropriate insert: 35mm dish or slide Match the red dots...
- Page 7 Install the chamber 3/3 5) Install an empty 35mm dish or a glass slide (to empty the hole) 6) Close the chamber putting back the glass lid 7) Connect the gas tube (pass the gas tube behind the condenser turret)
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Page 8: Switch On The Control Unit
Switch ON the control unit Switch on the device by pressing 5 sec the power button (all the components will start to heat) - Page 9 Set the appropriate insert Press: 4) Select: 1) Settings 1x35-M single (for 35mm dish) 1xGS-M single (for a slide) 2) Chamber & Insert 5) Press OK 3) Insert icon...
- Page 10 Objective heater settings !!! Remember to disable the objective heater if not needed!!! Press: 1) Settings 2) Obj. heater 3) Enable or disable 4) Press OK...
- Page 11 Install the sample !!! The device should be pre-warmed at least two hours before usage!!! 1) Install the sample in the insert 2) In case you are using an immersion objective add oil by removing the whole chamber...
- Page 12 Switch OFF the control unit 1/2 1) Switch off the device by pressing 5 sec the power button !!!If you press for less than 5 sec only the display will turn off!!! 2) Press OK...
- Page 13 Switch OFF the control unit 2/2 3) Close the Air and CO regulators (looking to the screen the regulators are on the left wall at the end of the LSM700 row) Down = open Up = close 4) Remove the sample 5) Disconnect the gas tube 6) Remove the chamber...
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