Optical Path Diagram (Tirf Microscopy) - Nikon TIRF Instructions Manual

2

Optical path diagram (TIRF microscopy)

TIRF attachment
(7) ND filter (ND16)
(6) ND filter (ND2, ND8, ND32)
(5) Collimator lens
(4) Optical-fiber cable
(1) Dichroic mirror
for combining
excitation light
(2) ND filter
(ND4, ND8)
Laser from
either of
the units
I Name and Function of Each Part
2 Optical path diagram (TIRF microscopy)
(11) Specimen
(10) Objective
TIRF cassette holder
(8) Condenser lens
(3) Motorized shutter
Two-laser unit
Optical path diagram
- 10 -
(9) Dichroic mirror
Detach the excitation filter.
(1) Dichroic mirror
for combining
excitation light
(2) ND filter
(ND4, ND8)
Three-laser unit
(3) Motorized shutter