4titude 4S2 User Manual page 16

Place the sealing film over the plate, ensuring that the sealing surface is face
down (Figure 6.A). When using heat sealing films that have a tendency to curl
(for example 4titude Peel Seal (product code 4ti-0521), DMSO Resistant Seal
(4ti-0587) and Clear Weld Seal Mark II (4ti-0575)) use the sealing frame which is
supplied with the 4ti-0625 adapter. For deep well plates place the weighted
platten on top of the sealing film (Figure 6.B). For most other plates we
recommend the use of the sealing frame (Figure 6.C).
Note: The sealing frame can only be used in conjunction with the 4ti-0625
adapter
Figure 6.A
Position sealing film on plate
Push the drawer in fully and hold until you can hear the motor pushing up the
lifting platform which activates the door locking mechanism. The microplate with
the sealing film will now be lifted up and brought into contact with the heating
element.
Note: If the drawer is not pushed in fully the locking mechanism may fail and the
motor will stop moving. An error message will be displayed and a continuous
audible beep will be emitted. To rectify this and to continue with the sealing
process push the drawer in fully until you can a beep. If the problem persists it
may be necessary to switch the unit off and then on again.
As soon as the sample plate makes contact with the heating element the display
shows the symbol "t" and the countdown of the pre-selected sealing time starts.
When the countdown timer reaches zero the unit emits an audible beep. The
motor returns to the home position and the drawer is released. The operator can
pull out the drawer and remove the sealed plate.
The unit will emit an audible beep as soon as the heating plate has reached the
pre-selected temperature again and a new cycle can be started within a few
seconds.
NOTE: Foil backed film may remain hot for a number of seconds after sealing.
Care should be taken in handling these plates.
Figure 6.B
Position weighted platten
above sealing film
Figure 6.C
Position sealing frame above
sealing film
Figure 7
Close the drawer
to start sealing.
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