Table Of Contents - Awareness Technology CHROMATE Operator's Manual

1. Introduction ...................................................................................................... 1
1.1 Applications .............................................................................................................................. 1
1.1.1 Intended Use ..................................................................................................................... 1
1.1.2 Summary of the Instrument ............................................................................................... 1
1.1.3 Principles of Operation ...................................................................................................... 3
1.2 Warning Markings Inscriptions d'avertissement ...................................................................... 4
1.2.1 Safety Symbols Le Symboles de Sûreté ........................................................................... 4
1.2.2 Safety Terms Terminologie de Sûreté .............................................................................. 4
1.2.3 Disposal and Storage ........................................................................................................ 5
1.3 Safety Precautions ................................................................................................................... 6
1.4 Operating Precautions ............................................................................................................. 8
2. Technical Specifications ................................................................................. 9
3. Installation ...................................................................................................... 10
3.1 Packing List ............................................................................................................................ 11
3.2 Installation Preparation .......................................................................................................... 11
3.3 Parts and Controls ................................................................................................................. 13
3.4 Instrument Setup & Software Installation ............................................................................... 15
3.4.1 Software Installation ........................................................................................................ 15
3.4.2 USB Port Connection ...................................................................................................... 17
3.5 Instrument Check Out ............................................................................................................ 18
4. Programming Assays .................................................................................... 19
4.1 General Selections ................................................................................................................. 19
4.1.1 Selecting a Plate Format ................................................................................................. 20
4.1.2 Loading a Plate ............................................................................................................... 21
4.1.3 Mix ................................................................................................................................... 21
4.2 Running an Assay .................................................................................................................. 22
4.2.1 Plate Layout Tab ............................................................................................................. 23
4.2.1.1 Reassign ................................................................................................................. 25
4.2.2 Assay Calibration Tab ..................................................................................................... 25
4.2.2.1 Viewing Statistics .................................................................................................... 28
4.2.2.2 Viewing a Curve ..................................................................................................... 28
4.2.2.3 Edit a Curve ............................................................................................................ 29
4.2.2.4 Deleting Standards (Calibrators) ............................................................................ 32
4.2.2.5 Adjusting Curves by Percentage Factor ................................................................. 33
4.2.2.6 Adjusting curves by running less than all the Calibrator ........................................ 34
4.2.2.7 View assay parameters .......................................................................................... 35
4.2.3 Add Samples Tab ............................................................................................................ 36
4.2.3.1 Sample Dilution Factor ........................................................................................... 37
4.2.4 Test Results Tab ............................................................................................................. 39
4.2.5 Report Tab ...................................................................................................................... 40
4.3 Tips for Running Assays ........................................................................................................ 42
4.3.1 Bichromatic Differential Operation .................................................................................. 42
4.3.2 Offset Absorbance ........................................................................................................... 42
4.4 Assay Modes.......................................................................................................................... 43
4.4.1 Absorbance ..................................................................................................................... 44
4.4.2 Factor Mode .................................................................................................................... 44
4.4.3 Standard Mode ................................................................................................................ 44
4.4.4 Cut Off Mode ................................................................................................................... 45
4.4.5 Point-to-Point Mode ......................................................................................................... 47

TABLE OF CONTENTS