Awareness Technology CHROMATE Operator's Manual
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Awareness Technology CHROMATE Operator's Manual

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© Awareness Technology, Inc. 2008 ChroMate Operator's Manual Revised 2018 Rev. Q

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  • Page 1 ® MICROPLATE READER ’ ’ © Awareness Technology, Inc. 2008 ChroMate Operator’s Manual Revised 2018 Rev. Q...

  • Page 3: Table Of Contents

    TABLE OF CONTENTS 1. Introduction ...................... 1 1.1 Applications ..........................1 1.1.1 Intended Use ........................1 1.1.2 Summary of the Instrument ....................1 1.1.3 Principles of Operation ...................... 3 1.2 Warning Markings Inscriptions d’avertissement ..............4 1.2.1 Safety Symbols Le Symboles de Sûreté ................4 1.2.2 Safety Terms Terminologie de Sûreté...
  • Page 4 4.4.10 4-Parameter and 5-Parameter Logistic ................. 50 4.4.11 % Absorbance Mode ..................... 51 5. ChroMate ® Manager Software ..............52 5.1 Opening ChroMate Manager ....................52 ® 5.1.1 Toolbar Icons ........................52 5.2 Management Menu ........................ 53 5.2.1 Test Count ........................54 5.2.2 New Assay Calibration Data ...................
  • Page 5 6.2 Errors and Flags ........................80 6.3 COM Port Settings ......................... 82 6.3.1 PC Communication (COM) Port Setting ................82 6.3.2 Communication (COM) Port Setting ................85 7. Contact Information ..................86 8. Appendix A – Laboratory Information System (LIS) ........87 9.

  • Page 7: Introduction

    1.1 Applications 1.1.1 Intended Use ® is a compact, PC-controlled, multi-purpose photometer system designed to ChroMate read and calculate the results of assays, which are read in 96 well microtiter plates or strip trays. General purpose software is included. ChroMate ®...
  • Page 8 ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 9: Principles Of Operation

    Using bichromatic differential absorbance values corrects for optical imperfections in the plastic wells and removes the effects of meniscus and turbidity. The photo-detectors generate electronic signals which are amplified and interpreted by the instrument circuitry. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 10: Warning Markings Inscriptions D'avertissement

    BIOHAZARD BIOHAZARDS are biological agents that can cause disease in humans. Lab workers handling potentially infectious materials must use universal precautions to reduce the risk of exposure to these agents. CAUTION! L’ATTENTION! ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 11: Disposal And Storage

    WARNING: Treat all components during use and disposal as you would any biohazardous material. AVERTISSEMENT : Utiliser et disposer des matériaux de la même manière que vous utilisé et disposer des matières infectieuses. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 12: Safety Precautions

    Do Not Operate Without WARNING: Do not operate this instrument with covers and panels Protective Covers removed. Avoid Exposed Circuitry WARNING: Do not touch exposed connections and components when power is present. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...
  • Page 13 If needed, a mild all-purpose or nonabrasive cleaner may be used. Use as a disinfectant a 10% solution of chlorine bleach or 70% • isopropyl alcohol. Take special care not to spill liquid inside the instrument • Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 14: Operating Precautions

    Avertissement! Lors du fonctionnement, si on renverse des matériaux, coupez immédiatement le courant. Placez le commutateur électrique a AU LOIN(0). Traitez le matériel comme biohazardous, utilisant approprie nettoient et des méthodes de disposition. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 15: Technical Specifications

    PC Connection: ........ USB Port Microprocessor: ........ eZ80 Speed: Reads absorbance of 96 wells in approximately 20 seconds Plate Transport: ........ Stepper motor Certifications: ........CE, ETL listed, NRTL listed (USA/CAN), Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...
  • Page 16 Technical Specifications (Continued) • Software: PC interface with ChroMate ® Manager software included (PC not included) Features: .......... PC interface software (included) with complete user prompting, error messages, full plate reporting, automatic interpretation options, duplicate options, controls locator, curve plotting and editing.

  • Page 17: Installation

    If power fluctuations or loss are frequent, an Uninterruptible Power Supply (UPS) is recommended. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...
  • Page 18 Avertissement! Pour la protection continue contre le risque du feu, remplacez FUSIBLE le fusible seulement par une du type spécifique et des estimations courantes. Démontez l’équipement de l’alimentation d’énergie avant de remplacer le fusible. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 19: Parts And Controls

    Figure 3.3-2 Well A1 location D. Lamp access door E. Universal Serial Bus (USB) connection F. External Protective Grounding Terminal G. Power switch I=ON O=OFF H. Power Cord Connection Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...
  • Page 20 Figure 3.3-3 Back of Instrument ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 21: Instrument Setup & Software Installation

    Turn on the computer and insert the installation CD or USB drive. The installer should start automatically. If not, or if the software is on a USB flash drive, locate the software by searching from the Windows start button. Double click to open the ChroMate ®...
  • Page 22 There are 2 important steps to take when installing and accessing ChroMate Manager software on Windows 8 or 10 computer systems. You must follow the steps below for all of the software ® features to be properly installed. When installing the Manager software, right click on the setup.exe file and select “Run As Administrator”...

  • Page 23: Usb Port Connection

    Driver installation - if installation of the software does not complete, minimize the installation windows in order to click OK on this screen: The PC COM port setting does not require an adjustment before activating ChroMate ® Manager. However, instructions on how to view and adjust the setting are provided in Section 6.3.1 PC Communication (COM) Port Setting.

  • Page 24: Instrument Check Out

    Follow the instructions under the Instrument Setup & Software Installation Section 3.3 to ensure that the ChroMate ® Manager software is installed and the USB cable is attached. Push the power switch to turn ON the computer and the instrument. Then double click the ChroMate Manager ® icon to start the software.

  • Page 25: Programming Assays

    10. Will a cut off value be used to label negative samples or define an equivocal zone? If so, results less than what cut off should be labeled as negative? Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 26: Selecting A Plate Format

    To use the plate map, a test must be programmed and stored. To read raw absorbance without programming a test, use the "Read Plate Absorbance" mode located under the Tests option on the main toolbar ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 27: Loading A Plate

    If the instrument firmware version is lower than AE:26, pressing the Mix button shows this message "Instrument firmware version does not support this feature. Please update the firmware!". Check with your instrument supplier. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 28: Running An Assay

    4.2 Running an Assay The ChroMate Manager Tabs are located at the bottom of the screen: ® Tab Title: Description: Item # Plate Layout Plate Layout is the default window. Refer to Section 4.2.1. Assay Refer to Section 4.2.2 Calibration Add Samples Refer to Section 4.2.3...

  • Page 29: Plate Layout Tab

    Read Plate button to run. Figure 4.2.1-1 Plate Layout Tab Figure 4.2.1-1 displays the current status of the instrument. Also shown is the currently loaded plate. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...
  • Page 30 (a total of 96 wells). Important note: assays run, or samples run against stored curves, must all have the same filter configuration when run in the same plate. Remove Removes well assignments. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 31: Reassign

    Figure 4.2.1.1-1 Manual Plate Assignment 4.2.2 Assay Calibration Tab CAUTION! CAUTION: Errors or alterations to calibration curves may introduce error to test results. ChroMate features ® are available for user convenience but may not safely apply to all test applications.
  • Page 32 Assay Calibration Tab (Continued) Figure 4.2.2-2 Assay Calibration Tab NOTE: When ChroMate is finished reading, select New Assay Calibration Data from ® Management on the toolbar, or by pressing the F8 key on your keyboard. This window shows whether the new curves and controls were accepted.
  • Page 33 (as shown above), click on the Accept Selected button to accept. The stored curve is displayed in the window. The stored curve will then be used for sample calculations. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 34: Viewing Statistics

    “Choose an assay” box to display the curve. If the curve was valid, it will be automatically accepted and shown in the lower right box as below. Figure 4.2.2.2-1 Graph of a curve ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 35: Edit A Curve

    (and generate a new curve) by adding the new calibrators and blanks. You should answer “yes” at a minimum when a new reagent lot is being used. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...
  • Page 36 Select the assay from the drop down list (Figure 4.2.2.3-3) and then click on the graph icon (1) and the stored calibration curve will display on the right hand side of the screen (2). Figure 4.2.2.3-3 Example Standard Curve Example ChroMate ® Microplate Reader Operator’s Manual Rev. Q...
  • Page 37 Moving the mouse pointer over the plotted curve will display the curve fit parameters. 4.2.2.3-4 View Curve fit parameters Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 38: Deleting Standards (Calibrators)

    If there are sample results, they will be updated on the Test Results tab. You can now choose to accept or delete any of the sample results by clicking on the buttons. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 39: Adjusting Curves By Percentage Factor

    NOTE: Decreasing the standard curve by a percentage will result in increasing the sample concentration values. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 40: Adjusting Curves By Running Less Than All The Calibrator

    Standard 5 is read individually. Figure 4.2.2.6-1 Adjusting a curve with one or more Standards By selecting this standard and all the stored standards, the new adjusted curve can be activated. Figure 4.2.2.6-2 Adjusted Curve ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 41: View Assay Parameters

    4.2.2.7 View assay parameters The parameters of an assay can be viewed by hovering the mouse cursor over the drop down arrow at the end of the window that displays the assay. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 42: Add Samples Tab

    Click on one or more of the numeric/sample ID’s on the left Tests to highlight, choose a test to run with the chosen IDs. Click on the test and press ‘Add Test’. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 43: Sample Dilution Factor

    NOTE: Do not use zero for dilution factor. The Dilution Factor is not stored with the plate data. If a Plate is stored and reloaded, the dilution factor must be set again. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...
  • Page 44 Test Results and Report tabs will use MDF=x.xxx under column Notes to indicate what dilution factor was used. If the Add to Plate button has been selected, the screen will be directed to Plate Layout. Figure 4.2.3.1-2 Dilution Factor can be reported under Notes ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 45: Test Results Tab

    Click ‘Accept’ to accept the results of the selected ID. buttons Press ‘Delete’ to discard the selected ID. NOTE: Results must be accepted to allow them to be viewed in the Report Tab. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 46: Report Tab

    Check this box to select all of the results. Only the selected results will appear in printouts. Sorting Select the Sorting Option from the drop down menu to Option change the way the results are displayed. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...
  • Page 47 (*.xls), or XML file (*.xml). Save for future reference. Remove Selecting the Remove button will remove all selected items from the results display. Retest Adds the selected items to the Sample Tab to be re-tested. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 48: Tips For Running Assays

    Select the Offset Absorbance field and enter the user-determined offset absorbance value. Press the Save button to save the settings. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 49: Assay Modes

    4.4 Assay Modes software contains several pre-programmed general-purpose calculations selected to ChroMate ® facilitate data handling for enzyme immunoassays and other similar tests. Access the Assay Modes via ChroMate ® Manager Tests Menu (refer to Section 5.2.2) •Absorbance •Factor •Standard •Cut Off...

  • Page 50: Absorbance

    The instrument first accepts a calibrator read singly or in multiple numbers and then calculates concentrations based on a single- point standard curve passing through the point (0,0). A blank is required to determine the (0,0) point. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 51: Cut Off Mode

    • Use this equation if only a negative control is used to determine the COV. COV = Y * mean (Pc) +F • Use this equation if only a positive control is used to determine the COV. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...
  • Page 52 (positive <= cut off, negative > cut off). The instrument calculates the mean of the positives and the mean of the negatives. User programmable QC Criteria is available on all controls. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 53: Point-To-Point Mode

    8.“Y=Log(1000*ABS), X=Log(Conc)” -- the base-10 log 0.0 concentration Calibrator is of the absorbance is plotted against the base-10 used, it will be ignored when log of the concentration the regression is calculated.) Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 54: Linear Regression Mode

    The resulting calibrator curve is a smooth curve connecting the calibrator points, which may be entered in ascending or descending order of absorbance. A constraining algorithm is applied to prevent curve overshoot. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 55: Dose Response Mode

    Fourth order is defined by: Y = A + B * X + C * X^2 + D * X ^3 + E * X ^4 Graphing and editing options are available. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 56: 4-Parameter And 5-Parameter Logistic

    (concave upwards to concave downwards). = Asymptote at infinite concentration: in an ELISA assay where you have a standard curve, this can be thought of as the response value for infinite standard concentration. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 57: Absorbance Mode

    The instrument accepts a number of calibrators read singly or in multiple numbers. Blanking, graphing, and editing options are available. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 58: Chromate ® Manager Software

    Power on the computer.  Power on the instrument and wait for the initialization to complete.  Double-click on the ChroMate icon to open the software. ®  NOTE: It is not necessary to turn the instrument off when restarting the software.

  • Page 59: Management Menu

    5.2 Management Menu Figure 5.2-1 ChroMate ® Manager Menu Bar Figure 5.2-2 Management Menu Option Description Connect Establishes or re-establishes communication between the software on the PC to the instrument. Communication Opens the interactive Communication Window used for certain Window diagnostics.

  • Page 60: Test Count

    5.2.2 New Assay Calibration Data When the instrument is finished reading, the New Assay Calibration Data can be viewed by ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 61: Tests Menu

    Blanks are menus used. Blank in wells data will display in the Plate Data table when the Read Plate button is selected. Figure 5.3.1-1 Read Plate Absorbance Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 62: Kinetic Read Using Read Plate Absorbance

    30 seconds, the instrument will not mix between reads. Read intervals need to be greater than 30 seconds and the ‘Mix between reads’ box checked before the instrument will mix between reads. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 63: Excel Export Using Read Plate Absorbance

    Raw Data worksheet, (row/column A1) followed by the second run (row/column A11) and so on. 12 x 8 Way checkbox allows user to define export format. • Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 64: Create Assay

    Use the ‘Properties’ button to make any adjustments to the Concentration or Minimum number of the standards entered. Figure 5.3.2.2-1 Assign Standard Properties If reading standards are read in duplicate, triplicate, etc., enter the number in the “Minimum number to use” field. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 65: Calibration - Curve Valid Time

    ‘Issue Warning’ or ‘Invalidate Tests’ as action to take when result is out of range, and to set the Valid Time, in days/hours. If no entry is made for Day(s) or Hour(s), expiration will be set at the default of (7) days. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 66: Calibration - Samples

    Set up the Out of Range Action and the Valid Time the Control(s).If no entry is made for Day(s) or Hour(s), expiration will be set at the default of (7) days. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 67: Calibration - Qc Criteria

    If there is also an (S) in the same equation it will represent the center variable. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 68: Interpretation Settings

    Click the Positive/Negative button and specify the ‘Positive greater than or equal to’ value, and ‘Negative less than’ value. Units may be typed in or drop down menu may be used. Figure 5.3.2.8-3 Positive/Negative ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 69: Edit Assay

    The ‘Print’ button sends data to a printer. • Save Assay The ‘Save’ button saves assay settings. 5.3.3 Edit Assay Edit Assay is accessed via the Tests Menu selection on the ChroMate Manager Menu ® Bar: Figure 5.3.3-1 Select Stored Assay Select the Assay from the drop down menu.

  • Page 70: Lot # Registration Menu

    Identify the Substance to be registered by clicking on the substance title from the dropdown menu. Click “Register” Enter the lot number, expiration date and description in the Note field (optional). Click OK to Save. Figure 5.4-1 Lot Registration window ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 71: Qc Tracking Menu

    NOTE: Lot numbers are entered in the tab labeled Lot # Registration. Edit by selecting the Select QC Points button or by un-checking the checkbox for specific QC points. Select the Save QC Points button to save the QC points displayed. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 72: Sample Database

    Modify a Sample ID record by clicking on the field to edit and typing. Delete a Sample ID by highlighting the record to delete and press the Delete • key on the keyboard. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 73: Modify Sample Id

    Highlight the items to be deleted then press the Delete key on the keyboard. A dialog box will open prompting the user to confirm the deletion of the record. Press Yes to delete, press No to cancel the deletion process. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 74: View Log

    Select the ‘Import’ button at the bottom of the Sample Database window. Select ‘Open’ to locate the text file to be imported. Select the data fields to be imported. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 75: Export Patient Record

    Enter criteria into Search Database area, press OK. All records containing the specified criteria will display. All records in the Patient Database can be displayed by pressing OK and leaving all search fields blank. Search result for “Smoker” displayed Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 76: Settings

    COM Port setting to Refer to match the COM Port Section 6.2 setting of the COM Port computer Settings Refer to Section 4.2.3.1 Refer to Section 5.7.1.1.1 International Sample Dilution Factor Calibration Factor (ICF) ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 77: International Calibration Factor (Icf)

    Click the OK button once all the selections have been made. NOTE: The Sample ID field and the Keyword field must be used. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 78: Result Acceptance

    Print logo and page header must be checked to have to include these features on reports. Logo and font size can be edited. A printed space can be added for notes and signatures. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 79: Report Output

    Report Type window, allows less paper to be Instead of one test or patient per page, multiple results will print on the allowable paper space. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 80: Custom Report Settings

    Tab and preview button. Preview sample reports via the Report Tab and preview button. Modify the example Custom Report and save it to a new file name. Figure 5.7.2.1-1 Custom Report Sample ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 81: Select Language

    A list of the available language choices will display. After selecting a language, it will be necessary to close the Manager and restart the software to initialize the selected language. To open the ChroMate Manager software, right click on the ChroMate icon and select “Run as Administrator”.

  • Page 82: Maintenance

    Acceptable voltage readings are within in the “greater than 2.0” and “less than 10.0” range. These values are for AFV (amplified filter voltages.). Blue range is < 7V Green range is 7 to 9V Red range is > 9V Figure 5.8.1-2 Voltage Meter ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 83: Firmware Update

    Figure 5.8.2-1 Password Required Figure 5.8.2-2 Firmware Update 5.8.3 Calibration Lock/Unlock The calibration settings for the ChroMate ® are set by the factory. The Calibration Lock/Unlock feature is locked at the factory as well. This feature is for emergency use only by authorized personnel in case the device would need to be recalibrated.

  • Page 84: Help

    5.9 Help The Help ‘About’ option provides a dialog window that displays the version of ChroMate ® Manager software and the version of firmware that is currently installed. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 85: Troubleshooting

    Customer Service”, it is very helpful for you to provide a file along with your email. Follow these instructions to create the “Root.zip” file: Click on "My Computer" and go to C:\ChroMate. • Right click on the file labeled "Root", select "Send To" and then "Compressed •...

  • Page 86: Errors And Flags

    Error "flags" appear as codes in the lower right hand corner of the screen. A flag is temporary and users may sometimes miss them. You can navigate to C:\ChroMate\Root\Logs on your PC and open the “MgrErr.log”. This log file will contain a history of any error messages that have appeared as well as an error code number.
  • Page 87 "Run as Administrator". If the error message returns, it may be the operating system security or the location of the setup file. Be sure to copy the ChroMate installation folder to the hard drive (C:) of the computer.

  • Page 88: Com Port Settings

    6.3 COM Port Settings Generally the communication (COM) port setting of the PC and the ChroMate will not require ® adjustment. However, the following steps are included just in case the user needs to view the setting or make an adjustment to the setting.
  • Page 89 Click on the Ports (COM & LPT) folder. You should see a version of Silicon driver . If Silicon Labs is NOT shown, recheck the connection between the instrument and the PC and be sure the instrument’s power switch is set to I (ON). Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...
  • Page 90 14 from the drop down list of options.in the Manager software. Be sure to select OK to exit at the bottom of the window. ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 91: Communication (Com) Port Setting

    Click to highlight the setting and then click on the OK button to save the setting. NOTE: The COM port setting will remain at this setting until it is changed. Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...

  • Page 92: Contact Information

    1935 SW Martin Hwy. Palm City FL 34990 USA Important: When contacting us, please have the Model and Serial Number of the ChroMate ® in question. Have a description of the problem with as much detail as possible. Save any relevant jobs or logs and send or e-mail us the information.

  • Page 93: Appendix A - Laboratory Information System (Lis)

    Results from the Requested Assay preceding. Indicates the End of the file. Append this information to the preceding record; may be used on any record. Figure 8.1-1 Record Type Definitions Microplate Reader Operator’s Manual Doc. 4300 Rev. Q ChroMate ®...
  • Page 94 NOTE: A solid bar (|) indicates the field is for future use. The bar delimiter is necessary for correct formatting within the record. File Examples: Figure 8.1-2 Import File Example (.LIS file extension) (.LIS file extension) Example Figure 8.1-3 Export ChroMate ® Microplate Reader Operator’s Manual Rev. Q...

  • Page 95: Appendix B - Installing New Software

    Using the computer’s Control Panel, uninstall any previous version of ChroMate ® Manager. Locate and double click on the ChroMate software folder. Right click on the setup.exe file and “Run as Administrator”. Follow the on screen instructions to install the software.

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