Membrane Lifetime; O2K-Multisensor Control And Calibration; Px Signal - Oroboros Instruments O2k Manual

MiPNet15.03
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4.8. Membrane lifetime

5.

O2k-MultiSensor control and calibration

5.1.

pX signal

Graphs
Oroboros Instruments
O2k-MultiSensor-ISE
If the required performance criteria are not met, the
following steps should be tested:
Set the polarisation voltage of the OroboPOS to 0.
Observe any effects on the pX raw signal. A tiny
potential jump is acceptable. If a drift in the pX signal
is either increased or reduced by this test or an
extreme jump in the signal observed, the membrane of
the OroboPOS
polarisation voltage to 800 mV after the test.
Shake the electrode as described above to make sure
that no air bubble is trapped at the tip of the electrode.
Condition the electrode for a longer time in storage
solution.
Repeat the entire conditioning process, starting with
conditioning solution 1.
Replace the membrane.
Under experimental conditions, the
membrane is primarily determined by exposure to
organic solvents or inhibitor accumulation in biological
experiments.
different applications.
replaced when
satisfactory.
can be constructed to include both, recorded oxygen
and pX, or several graphs can be added to display
oxygen and pX data separately. All graph settings can
be saved as user-defined layouts (MiPNet19.18C).
should be replaced.
These factors vary considerably in
A membrane should only be
the performance
Mitochondria and cell research
18
Reset the
lifetime of a
is no longer