Oroboros Instruments O2k-Core Manual
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Oroboros Instruments O2k-Core Manual

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OROBOROS O2k-Core Manual
Mitochondrial Physiology Network 19.18(B04):17-22 (2016)
Version B04: 2016-08-08 DatLab 7 2014-2016 OROBOROS
B:
http://wiki.oroboros.at/index.php/MiPNet19.18B_POS-Service
Service of the
polarographic
oxygen sensor
OroboPOS
Gnaiger E
OROBOROS INSTRUMENTS
Schöpfstr 18, A-6020 Innsbruck, Austria
Email:
instruments@oroboros.at
www.oroboros.at
Section
1.
OroboPOS-Service Kit ............................... 18
2.
Cleaning ................................................. 18
3.
Membrane mounting ................................ 20
4.
Electrical cable connection ........................ 21
5.
Storage of the OroboPOS .......................... 21
6.
7.
References .............................................. 22
Summary
Service of the polarographic oxygen sensors
POS) is the basis of signal stability, low noise and high time resolution.
Performance specifications of the OROBOROS O2k can be met only with
oxygen sensors which are maintained in a well monitored functional state.
(5) the oxygen signal at zero calibration does not decline rapidly to 0% to
max. 5% of the signal at air saturation. For each sensor, the
frequency of POS service is optimized on the basis of long-term
calibration records for quality control (O2-calibration.xlsx).
OROBOROS INSTRUMENTS
POS service may not be required
for several months of operation.
POS service is necessary if (1) a
new sensor is prepared, (2) the
signal at air calibration is not stable
over time, (3) signal noise is high,
(4) the time response is prolonged
and biphasic (time constant >10 s),
high-resolution respirometry
B
Page
(OroboPOS,

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Summary of Contents for Oroboros Instruments O2k-Core

  • Page 1: Table Of Contents

    OROBOROS O2k-Core Manual Mitochondrial Physiology Network 19.18(B04):17-22 (2016) Version B04: 2016-08-08 DatLab 7 2014-2016 OROBOROS http://wiki.oroboros.at/index.php/MiPNet19.18B_POS-Service Service of the polarographic oxygen sensor OroboPOS Gnaiger E OROBOROS INSTRUMENTS Schöpfstr 18, A-6020 Innsbruck, Austria Email: instruments@oroboros.at www.oroboros.at Section OroboPOS-Service Kit ....... 18 Page Cleaning ..........

  • Page 2: Orobopos-Service Kit

    Remove the OroboPOS- 3. Pull the PEEK membrane ring from membrane ring 2,3 and membrane the sensor head Wash off electrolyte with distilled water. Removal of a used membrane Clip B2 from the OroboPOS. O2k – tested and trusted OROBOROS INSTRUMENTS...
  • Page 3 With severely aged sensors it is necessary to prolong the exposure to ammonia up to several hours (overnight), sealing the ammonia under a membrane and under the POS cover slip. Protect the POS from light, since the silver/silver chloride anode is light sensitive. high-resolution respirometry OROBOROS INSTRUMENTS...

  • Page 4: Membrane Mounting

    4 To slide the OroboPOS- Membrane Ring over the POS head, slide the membrane ring holder into the membrane guide, and press firmly down in a single movement to the final position. O2k – tested and trusted OROBOROS INSTRUMENTS...

  • Page 5: Electrical Cable Connection

    This ensures a longer life time of the sealing rings. The receptacle of the stopper is completely filled with ethanol from the top, and is sealed with a black cover slip to avoid high-resolution respirometry OROBOROS INSTRUMENTS...

  • Page 6: Replacement Of The Orobopos Head

    Hitchman ML, Gnaiger E (1983) A thermodynamic consideration of permeability coefficients of membranes. In: Polarographic oxygen sensors, Gnaiger E and Forstner H, eds., Springer, Berlin, Heidelberg, New York:31-6. » Updates »http://wiki.oroboros.at/index.php/MiPNet19.18B_POS-Service »http://wiki.oroboros.at/index.php/MiPNet19.18A_O2k-Start Next step – O2k-Core Manual D »MiPNet19.18D O2k-Calibration O2k – tested and trusted OROBOROS INSTRUMENTS...

  • Page 7: Supplement: Videosupport

    B1_OroboPOS from Perspex housing  B2 Disassembly of OroboPOS  B3 Cathode cleaning  OroboPOS-Polishing Cloth, OroboPOS-Polishing Powder. B4 Anode cleaning  B5 Membrane mounting  Supplement B. Links to O2k-Catalogue » http://wiki.oroboros.at/index.php/OroboPOS » http://wiki.oroboros.at/index.php/O2k-Catalogue:_OroboPOS_components » http://wiki.oroboros.at/index.php/OroboPOS-Service_Kit high-resolution respirometry OROBOROS INSTRUMENTS...

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