Oroboros Instruments O2k-FluoRespirometer Manual

O2k-FluoRespirometer manual
Mitochondrial Physiology Network 22.11(01):1-16 (2017)
Version 01: 2017-07-21 O2k-Series H DatLab 7 2017 Oroboros
wiki.oroboros.at/index.php/MiPNet22.11_O2k-FluoRespirometer_manual
O2k-FluoRespirometer
manual
Gnaiger E 1,2 , Sigl R 1 , Mészáros A
Capek O 1 , Gradl L 3 , Gradl P
Oroboros Instruments
Schöpfstr 18, A-6020 Innsbruck, Austria
Email: instruments@oroboros.at
www.oroboros.at
Mitochondria and Cell Research
Section 1. Unpack the O2k .......................................... 3
2. Oroboros USB-flash drive and DatLab ............ 3
3. Electric and lab space requirements ............... 4
4. Start the O2k .............................................. 6
5. Assembly of the O2k .................................... 6
6. Start DatLab ............................................... 9
7. O2k control ............................................... 14
Supplement A: Videosupport ............................. S1
Supplement B: O2k-Chamber assembly .............. S2
Supplement C: O2k-Chamber volume calibration . S3
Overview
The Oroboros O2k-FluoRespirometer provides a unique high-resolution
approach to the monitoring of cellular and mitochondrial respiratory function.
High-Resolution FluoRespirometry (HRFR) is indispensable for application of
SUIT protocols, at low respiratory activities, kinetic transitions, and controlled
oxygen levels. High-resolution is required for analysis of pathological effects
causing reduced respiration (mitochondrial and metabolic diseases,
ischemia-reperfusion injury, oxidative stress, apoptosis, aging). HRFR is
unique for simultaneous measurement of respiration and fluorometric
signals (H O
2 2
phosphorylation), for analysis of biopsies with limited amounts of sample
(diagnosis of genetic and acquired mitochondrial defects in pediatric
patients, diabetes); cell cultures with limited cell numbers or low activities;
mutants with diminished respiratory capacity; chemical oxidation rates and
antioxidant capacities
physiological intracellular oxygen levels.
Oroboros Instruments
1 ,
4
flux, mt-membrane
and oxygen
O2k-FluoRespirometer
potential, Ca
kinetic measurements
High-Resolution FluoRespirometry
Page
2+ , ADP-ATP
at low,