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Diatron Abacus + Service Manual

Hematology analyzer
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Hematology Analyzer
Service Manual
DIATRON Messtechnik Ges.m.B.H.
A-1141 Wien, Ameisgasse 49-51/2.
Tel.: (431) 914-85-00, 911-38-48
Web:
H-1038 Budapest, Papírgyár u. 58-59.
Web:
E-mail: support@diatron.com
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Release 1.09
AUSTRIA
Fax: 914-85-07-15
www.diatron.com
DIATRON MI Ltd.
HUNGARY
Tel.: (361) 436-98-00
Fax: (361) 436-98-00
www.diatron.com
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  • Page 1 Hematology Analyzer Service Manual Release 1.09 DIATRON Messtechnik Ges.m.B.H. A-1141 Wien, Ameisgasse 49-51/2. AUSTRIA Tel.: (431) 914-85-00, 911-38-48 Fax: 914-85-07-15 Web: www.diatron.com DIATRON MI Ltd. H-1038 Budapest, Papírgyár u. 58-59. HUNGARY Tel.: (361) 436-98-00 Fax: (361) 436-98-00 Web: www.diatron.com E-mail: support@diatron.com...
  • Page 2 Diatron MI PLC Revision history: Revision Section Modification 1.00 PRELIMINARY Support 10-11.2006 Team 1.09 Updating tubing schematic Cust.Serv May 2007 rev 1.09...

  • Page 3: Table Of Contents

    Abacus Service Manual TABLE OF CONTENTS INTRODUCTION ............................7 1.1........................7 AME AND SERIAL NUMBER 1.2............................7 NTENDED USE 1.3.......................... 8 NTEGRATED SOFTWARE FUNCTIONAL DESCRIPTION ......................... 9 2.1................... 9 AIN ELECTRONIC PARTS OF THE ANALYZER 2.1.1. Counting chambers with electrodes and measuring apertures ...........
  • Page 4 Diatron MI PLC CHECKING THE PROPER OPERATION ..................... 49 5.1.............................. 49 ELF TEST 5.1.1. Self test Screens ........................... 49 5.1.2. Normal range of Self Test parameters ..................50 5.1.3. Troubleshooting Guide for Self test ..................... 50 5.2............................51 ERVICE 5.2.1.
  • Page 5 Abacus Service Manual APPENDICES ............................80 10.1............................ 80 ARNING FLAGS 10.2....................82 ERIAL OMMUNICATION ROTOCOL 10.2.1. General Description ........................82 10.2.2. Format of Packages Sent ......................83 10.2.3. Format of Acknowledge of the Receiver ..................83 10.2.4. Detailed Description of Packages ....................83 10.2.5.
  • Page 6 Diatron MI PLC rev 1.09...

  • Page 7: Introduction

    Abacus Service Manual INTRODUCTION Although Abacus+ is a member of Diatron‟s Abacus junior instrument, it has special and different characteristics compared to other junior family members. We issue a dedicated Service Manual for this instrument; information herein applies for Abacus+.

  • Page 8: Integrated Software

    Every Abacus+ software version is upgradeable (using an USB drive) by the latest program developed by DIATRON, and it can be downloaded from: www.diatron.com...

  • Page 9: Functional Description

    Abacus Service Manual FUNCTIONAL DESCRIPTION 2.1. Main electronic parts of the analyzer Abacus+ contains the following electronic parts: Counting chambers with electrodes and measuring apertures HGB Measuring Head Cell Counter Amplifier Board (behind the chambers) CPU Board with DIMM-PC and measurement processing unit (COMB Board) Pneumatic and Power Board (PPB) with motor controllers, valve &...

  • Page 10: Counting Chambers With Electrodes And Measuring Apertures

    Diatron MI PLC 2.1.1. Counting chambers with electrodes and measuring apertures Impedance method is used for determination of volume and number of cells. In this method a known volume of dilution is drawn through a small aperture. Constant current is passed through the aperture from one side to the other.

  • Page 11: Cell Counter Amplifier Board

    Abacus Service Manual The analyzer performs enhanced Hemoglobin measurement technology for HGB measurement. The output of HGB head is frequency (TSL235 detector is light to frequency converter). This signal is counted by a digital counter in the FPGA circuit/micro-controller. This counter counts up while the LED is on and counts down while the LED is off, the LED and the counter directions are switched with a 100 Hz signal.

  • Page 12: Control And Measurement Board (Comb) With Dimm-Pc Core

    Diatron MI PLC Amplifier board includes a 3-stage main amplifier channel, which gains to the input signal to the 0...5 V range (this is the input range of the A/D converter, which is placed on the COMB card). There is an offset potentiometer, P1 in the third amplifier stage, manufacturer sets the correct offset voltage.

  • Page 13: Dimm-Pc* Module

    Abacus Service Manual 2.1.5. DIMM-PC* Module The MB4 board incorporates a credit-card sized PC, named DIMM-PC*. The processor on the DIMM-PC is a 133MHz Pentium-class core, with 32Mbytes on-board RAM, and 32Mbytes on-board FlashDisk. This is the HDD (hard disk drive) of the analyzer, so instrument software with all user settings, calibration, database, etc.

  • Page 14: Pneumatic And Power Board (Ppb)

    Diatron MI PLC 2.1.7. Pneumatic and Power Board (PPB) PPB card contains the main power regulator circuits, valve and motor driver circuits and other connections for the fluidic and pneumatic system‟s parts. PUMP MDIL X_Y_SR Peristaltic pump Micro dilutor Horizontal, Vertical &...

  • Page 15: Opto-Boards For Stepper Motors

    Abacus Service Manual The green one has 3 states: dark: (after initialization phase) error state, blinking: communication in progress - normal state on(just lighting): OK - normal state 2.1.8. Opto-boards for stepper motors There are five stepper motors in the system: Horizontal and Vertical motors, which make the movements of the sampling needle;...

  • Page 16: Pressure Sensor

    Diatron MI PLC 2.1.10. Pressure Sensor This is an MPX5100AP calibrated pressure sensor, which can measure the required air pressure and vacuum. The Pressure Sensor is connected directly to the PPB card. Connection to Puffer reservoir Connection to: The pressure sensor can operate from +5V only. It is a calibrated sensor with 0-1.1 Bar input range.

  • Page 17: Lcd Display Module With High Voltage Board

    Abacus Service Manual 2.1.12. LCD Display Module with High Voltage Board LCD & Keypad controller LCD module Start key connection and status LED LCD backlight lamp Keypad connector Display assembly contains the 240x128 dots graphics LCD display and the high voltage board.

  • Page 18: Keypad

    Diatron MI PLC 2.1.13. Keypad The analyzer has a 29-button foil keypad including numerical keypad (0-9, “.”), cursor moving, OK and Del buttons, and 6-6 function buttons, above and under the LCD display as it is shown in the picture below:...

  • Page 19: Main Mechanic And Fluidic Parts Of The Analyzer

    Abacus Service Manual 2.2. Main mechanic and fluidic parts of the Analyzer Abacus+ Hematology Analyzers consist of the following mechanic and fluidic parts: 1. Sampling needle 2. Washing head 3. H&V moving unit 4. Micro Dilutor 5. Dilutor 6. Chambers 7.

  • Page 20: Sampling Needle

    Diatron MI PLC 2.2.1. Sampling needle Sampling needle is assembled in the H&V moving unit and it makes the sample aspirations. Correct setting of sampling needle is necessary and very important (see Chapter Adjustments). 2.2.2. Washing head Washing head is located at the bottom of the H&V moving unit and it is for cleaning the outer surface of the sampling needle.

  • Page 21: H&V Moving Unit

    Abacus Service Manual 2.2.3. H&V moving unit This unit contains slides to move the sample sampling needle in Horizontal and Vertical directions, two stepper motors, XYR opto board, opto wheel, washing head and the sampling needle. It moves the needle to the desired position: from sampling position, to washing head, and to the measuring chamber.

  • Page 22: Main Dilutor

    Diatron MI PLC 2.2.4. Main Dilutor There are two stepper motors, a common motor opto board, four syringes and piston rods with gear transmission. Maintenance should be provided to the piston tips, by applying neutral silicon grease to the cogged end of the Macro and Lyse pistons, between the syringe and the tip itself.

  • Page 23: Micro Dilutor

    Abacus Service Manual 2.2.5. Micro Dilutor Micro dilutor is taking the precise sample (25 or 50μl) into the sampling needle. It includes a stepper motor, a motor opto board and the micro syringe. Connection to Valve Connection to sampling needle Connection to PPB Motor opto board Flexible clutch...

  • Page 24: Assembled Analyzer

    Diatron MI PLC 2.3. Assembled Analyzer 2.3.1. Abacus+ Front Panel Built-in thermal printer (optional) 240x128 dots Graphic LCD Foil keypad Function keys START button Rear panel: Power switch 12V DC inlet Warning labels USB ports Serial Port USB slave port...
  • Page 25 Abacus Service Manual Construction – front: Valve block Pressure sensor Electronic block H&V unit Speaker Valve block Construction – right side Valve block Needle moving mechanics Reagent sensor Puffer reservior Microdilutor Measuring chambers Pump Valve block Dilutor block rev 1.09...
  • Page 26 Diatron MI PLC Construction – left side: Pneumatic and Power Board (PPB) ID Eprom Board (IDEPROM) Control and Speaker Measurement Board (COMB) rev 1.09...

  • Page 27: Adjustment

    Abacus Service Manual ADJUSTMENT Mechanical and hardware adjustments are described in this section. Software settings are included in Section 5.2. 3.1. Mechanical settings There are two important mechanical settings in the system: Opto wheel setting (Vertical motor) Sampling needle setting The manufacturer adjusts the analyzer during production.

  • Page 28: Sampling Needle Setting

    Diatron MI PLC 3.1.2. Sampling needle setting This adjustment sets the sampling needle to the operational position. In Service menu, in Miscellaneous submenu of AJ/AJvet and in Service menu of AJB select Needle setting. The software moves the needle back and up, and turns on horizontal and vertical motors (AJ/AJvet) to keep needle in place.

  • Page 29: Operation Of The Fluidic System

    Abacus Service Manual OPERATION OF THE FLUIDIC SYSTEM This section describes the main steps of Abacus+ fluidic functions. The instrument‟s Fluidic Schematics are shown in section 2.2 of this manual. The following figures show total measurement flow diagram and detailed descriptions of basic processes for understanding the fluidic system work.

  • Page 30: Basic Processes

    Diatron MI PLC 4.1. Basic processes 4.1.1. Sampling process The aspirating needle aspirates 25 µl (50 µl in prediluted mode) of blood sample. The Micro- dilutor syringe makes the aspirating while the M5 Micro-dilutor motor moves down. There is another sampling process for the second (RBC) dilution, when 35 μl of primary dilution is aspirated from the MIX by the aspirating needle.

  • Page 31: Needle Washing Process

    Abacus Service Manual 4.1.2. Needle washing process The instruments clean the sampling needle with diluent in the washing head after sampling. It is important to clean the outer surface of the sampling needle to avoid inaccurate sampling. The Macro syringe doses and the pump drains the diluent from the washing head, while the sampling needle moves upwards so that the total length of it is washed and cleaned.

  • Page 32: Diluting Processes

    Diatron MI PLC 4.1.3. Diluting processes Before the dilutions the MIX and the RBC chambers are filled up with 1 ml of diluent. This method prevents the chamber from dirt and makes the diluting process faster. The sampling process has aspirated 25µl of sample (or 35µl of primary dilution), which is in the sampling needle.

  • Page 33: Fluid Transfer From Mix Chamber To Wbc Chamber

    Abacus Service Manual 4.1.4. Fluid transfer from MIX chamber to WBC chamber In Abacus+, during this step the liquid remaining in the mixing chamber flows via the V0 (On) into the WBC chamber, because the vacuum – which is in the puffer reservoir and by the pump –...

  • Page 34: Lysing Process

    Diatron MI PLC 4.1.5. Lysing process During this step the primary dilution remaining in the mixing chamber flows into the WBC chamber, like a simple MIX WBC transfer, but in this case simultaneously, 0.8 ml of lysing reagent is added through V9 (On), while the Lyse syringe moves upwards. This process makes the 1:192 dilution rate and good mixing with lyse.

  • Page 35: Counting Process

    Abacus Service Manual 4.1.6. Counting process The regulated vacuum (it is generated by the pump in the puffer reservoir) aspirates the diluted sample (WBC and RBC) from the chamber through V2 (On) valve. The instrument counts the cells for 5 seconds in both counting phases (at first WBC and after it the RBC). rev 1.09...

  • Page 36: Aperture Priming Process

    Diatron MI PLC 4.1.7. Aperture priming process In this process the aperture branch and backsides of the apertures are filled with diluent and . After generating vacuum in the puffer reservoir the pump continues the drainig of the aperture branch (V2 and V3 On, V1 and V5 Off) meanwhile the diluent syringe of M4 dilutor unit pushes diluent through V10 (On), V6 (On) and V8 (On).

  • Page 37: Wbc Chamber Draining Process

    Abacus Service Manual 4.1.8. WBC chamber draining process The WBC chamber draining is performed via V3 (On) and V4 (On) valves by the pump while the V5 must be off. The WBC chamber draining is always executed without using the puffer reservoir.

  • Page 38: Rbc Chamber Draining Process

    Diatron MI PLC 4.1.9. RBC chamber draining process The RBC chamber is drained by the vacuum in the puffer reservoir through V1 (On) valve (V2 must be off). This vacuum is made (and maintenated) by the peristaltic pump. During the draining the pump can perform either other task like WBC chamber draining or needle wash, or can help the draining of the RBC chamber.

  • Page 39: Cleaner Priming Process

    Abacus Service Manual 4.1.10.Cleaner priming process The cleaner solution is primed by vacuum in the following way: V7 (On), Cleaner Detector, V6 (On), back side of the RBC aperture, back side of the WBC aperture, V2 (On), V3 (On), V4 (On) and the peristaltic pump. The V1 and V5 valves are off. The flow of the cleaner is controlled by the vacuum, generated in the puffer reservoir before the start of the priming.

  • Page 40: Main Functions Of Fluidic System

    Diatron MI PLC 4.2. Main functions of fluidic system 4.2.1. Initialization Fluidic initialization process performs the following steps: Positioning all mechanical components (stepper motors) by scanning moving range (with end-switches) Checking the atmospheric pressure Checking of pump and pressure sensor by generating measuring vacuum...

  • Page 41: Measurement Cycle

    Abacus Service Manual 4.2.3. Measurement cycle Before the start of the measurement cycle the instrument is in ready state (status LED is green). In this state the sampling needle is in the sampling position: out and down. The Mix chamber is empty, the WBC chamber contains 5.0 ml of diluent (the wake up process or previous measurement put it into the chamber and the blank HGB measured with this liquid used).
  • Page 42 Diatron MI PLC First dilution (MIX chamber) Dilutior (M3 ) RBC sampling moves down Needle (short) washing Dilutior (M3) Needle positioning moves down over the RBC chamber Second dilution (RBC chamber) Dilutior (M3) moves down Needle up rev 1.09...
  • Page 43 Abacus Service Manual Microdilutor syncronization Fill up the MIX chamber with 2.5ml of diluent Fill the MIX chamber with 2.5ml of diluent Dilutior (M3) moves down Perform wake up process rev 1.09...
  • Page 44 Diatron MI PLC Chamber process HGB blank delay Dilutor (M4) moves down and primes diluent Measuring HGB blank RBC chamber Fill up the MIX draining chamber with 1 ml of diluent Dilutor (M4) moves down and primes diluent Fill up the RBC...
  • Page 45 Abacus Service Manual Dilutor (M4) moves down and primes diluent Aperture priming Generating measuring vacuum WBC counting RBC counting HGB measurement RBC chamber WBC chamber draining draining Pull out 0.2 ml of Lyse Autoclean? Fill up the RBC Fill up the RBC chamber with 0.8 ml chamber with 2.5 ml of diluent...
  • Page 46 Diatron MI PLC MIX WBC Autoclean? Cleaner Priming WBC chamber Fill up the MIX draining chamber with 2.5 ml of diluent and cleaner Fill up the RBC chamber with diluent Aperture Priming MIX WBC High voltage cleaning Dilutor (M4) moves down and primes pulses;...

  • Page 47: Standby

    Abacus Service Manual MIX WBC Autoclean? Dilutor (M4) moves down and primes diluent High voltage cleaning pulses; backflush Aperture Priming Dilutor (M4) moves down and primes diluent 4.2.4. Standby Standby process is executed when the instrument is in ready state and the standby time (see Service Settings) ellapsed In the beginning of the standby process the needle goes up and is washed and the chambers are drained.

  • Page 48: Cleaning

    Diatron MI PLC 4.2.5. Cleaning Cleaning function drains all of the chambers and fills the RBC chamber and the MIX chamber with 2.5 ml of diluent. After moving content of the MIX chamber into the WBC chamber the aperture branch is primed with cleaner (see 1.x). The remaining cleaner between the cleaner detector and the V6 valve is pushed into the MIX chamber with diluent.

  • Page 49: Checking The Proper Operation

    Abacus Service Manual CHECKING THE PROPER OPERATION There is a built-in Self test and Service menu in the analyzer. 5.1. Self test 5.1.1. Self test Screens Self test can be used to check the operation of the instrument. The first panel shows general information about the instrument.

  • Page 50: Normal Range Of Self Test Parameters

    Diatron MI PLC 5.1.2. Normal range of Self Test parameters Parameter Unit Lower bound Upper bound HGB light count 3000 60000 HGB dark Count 3000 Electrode voltage µA Current Offset -5.0 Amplifier test count 19990 20005 Peak of test pulses...

  • Page 51: Service Menu

    Abacus Service Manual 5.2. Service Menu 5.2.1. Entering to Service Menu There is a Service menu for servicing and operation checking purposes. The entry point is in the User‟s Service screen, where Service Information is displayed. Enter the code to access Service menu: 6484 5.2.2.

  • Page 52: Service Calibration

    Diatron MI PLC 5.2.5. Service Calibration The analyzer provides a menu for Service calibration purposes. In result calculations the service calibration factors are used as the user calibration factors, so they are multiplied for each parameter: RBC = Fact * Fact * RBC Disp.
  • Page 53 Abacus Service Manual Stretch histogram: setting this option to Yes will modify the WBC histogram range from 0- 300 fl instead of 0-400 fl (this is recommended for Veterinary mode). Reagent code: This code can be used to modify the offset of the HGB function. Enter a value between 0 and 30 (default is 9).
  • Page 54 Diatron MI PLC 5.2.6.2. Operation Settings LCD light off delay: LCD backlight switches off after 4 hours, by pressing key switches back. Still the light is off, the LED is flashing yellow. Disable multi-user mode: by this setting the multi-user mode can be disabled...

  • Page 55: Service Testing Menu

    Abacus Service Manual 5.2.7. Service Testing Menu Service Testing menu provides tools for checking hardware. 5.2.8. Valve Test Menu In the menu on the left you can see the valve numbers and a number under each that represents the actual state of the valve.

  • Page 56: Display And Keyboard Test

    Diatron MI PLC 5.2.10. Display and Keyboard Test Display and keyboard test is provided to check keypad and LCD panel. Press a key to test and the LCD will show the code of the pressed key, and will be invert the colors at each touch of a key for testing that every LCD dot is functioning.

  • Page 57: Miscellaneous Settings

    Abacus Service Manual 5.2.12. Miscellaneous Settings In this menu, you can directly perform Software upgrade (this will restart the instrument). Clear Device Statistics: Device statistics (number of measurements, aperture-clogging and other errors) can be cleared. Clear Database: whole Database (measurement results including histograms) can be cleared.

  • Page 58: Service Operation

    Diatron MI PLC SERVICE OPERATION 6.1. Opening the instrument On the right side of the instruments there is a side door, which allows reaching of the fluidic system and the mechanical parts. Other parts of the analyzer (electronic parts, etc.) can be reached by opening the top-left cover.
  • Page 59 Abacus Service Manual (In MDA mode, continuous changing of screen may appear. To avoid this, press left and up arrow on the keypad in any order.) Legacy Diskette A: 1.44/1.25 MB Primary Master: FlashDisk SDTE-XX DVANCED – PNP OS Installed: –...

  • Page 60: Checking The Bios Setup

    Diatron MI PLC 6.4. Checking the BIOS setup It is suggested to check it if the instrument hangs after switching on, or software does not start (or cannot be upgraded from floppy disk). Connect an external keyboard (US layout) to the instrument.
  • Page 61 Abacus Service Manual Key Function <F1> or <Alt-H> General help window <Esc> Exit this menu left or right Arrow key Select a different menu up or down Arrow key Move cursor up and down <Tap> or <Shift-Tap> Cycle cursor up and down <Home>...

  • Page 62: Dos Functions On The Instrument

    Diatron MI PLC 6.6. DOS functions on the instrument To run the computer part of the instrument like a normal PC, the Service Personnel should have a floppy drive with cables (see chapter 9.5), and an MS-DOS 6.22 operating system boot floppy disk.

  • Page 63: Troubleshooting

    Abacus Service Manual TROUBLESHOOTING The analyzer checks the operations of several mechanic, fluidic and electronic parts during measurement. The system shows the type of the error on the LCD display if any kind of malfunction is detected. The electronic parts have a very little chance to fail, only the connections and cables could disconnect, which can cause the malfunction of the electronic system.

  • Page 64: Error Codes

    Diatron MI PLC 7.1. Error codes %s: filename concerned, %d: error type concerned, %u: error specific string 7.1.1. Software/system errors Code Message Reason(s) Remedy Error %d on opening file Fatal system or The same as in case of error 1001 program error! 1000.

  • Page 65: Measure Errors

    Abacus Service Manual Vacuum error The measuring vacu- Leakage in the pneumatic system um has been (check the pump, fittings, tubings, dramatically pressure sensor, puffer reservoir 1105 decreasing during and valves). the measurement or absolutely lost! 1106 Pneumatical error Fatal error in If frequently happens this error occurred! mechanical...

  • Page 66: Printing Errors

    Diatron MI PLC 7.1.4. Printing errors Code Message Reason(s) Remedy 1400 Printer error %d Printer error! Check the connections between the instrument and the printer and check the printer setup in the Settings/Printer settings sub-menu. 1401 Printer is out of paper Printer is out of paper Feed the printer with paper.

  • Page 67: Other Errors

    Abacus Service Manual 7.1.5. Other errors Code Message Reason(s) Remedy You have to add at least Multi-user problem Follow the instruction, or change 1900 one user to use multi to single user mode in the User user mode! settings submenu. %s sensor calibration Reagent container Replace the reagent container...

  • Page 68: Warning Messages

    Diatron MI PLC 7.2. Warning messages 7.2.1. Pneumatic warnings Code Reason(s) Remedy Message 5001 First message of Remove the reagent tubing and Remove reagent tubing at rear reagent inputs preparing for leave waste connected. (Diluent, Lyse, shipment. After pressing the analyzer Cleaner).

  • Page 69: Database Relating Warnings

    Abacus Service Manual 7.2.2. Database relating warnings Code Reason(s) Remedy Message 5101 data record(s) There are %u data Press selected. records present in the database which match the selection. 5106 There are no %u Press and check the No such type of data data records present selection.

  • Page 70: Hardware Relating Warnings

    Diatron MI PLC 7.2.5. Hardware relating warnings Code Reason(s) Remedy Message 5400 The +12V is not Contact the Service Personnel. +12V too low! sufficient, the measurements are unreliable. 5401 The -12V is not Contact the Service Personnel. -12V too low!

  • Page 71: Possible Causes Of Noise

    Abacus Service Manual 7.3. Possible Causes of Noise Generally high count of any particle - even if you think it should be low, or near zero - can be caused by NOISE, i.e. something interferes with measurement. The most important thing in these cases to identify the source of NOISE, otherwise you cannot protect the system against it.

  • Page 72: External Electrical Noise

    Diatron MI PLC 7.3.3. External electrical noise If another instrument is near the analyzer can radiate electromagnetic signals in the 1 kHz - 100 kHz frequency region it can be picked up by the system (especially if they are very close to each other, or the grounding is not quite perfect).
  • Page 73 Abacus Service Manual 7.3.4.2. Bad insulation of electronic signal paths: In these cases check for any capacitive coupling of electronic signals to the chamber: interference with HGB head (high-frequency signal is coupled to the chamber). HGB head metal parts must be grounded. The ground comes externally, it must be in place, otherwise HGB head does not shield, but couples in noise.

  • Page 74: Maintenance

    Diatron MI PLC MAINTENANCE 8.1. Weekly Maintenance by User User should carry out on the first workday, before starting up the analyzer. 8.1.1. Cleaning the washing head User should clean the lower surface of the sampling needle washing head using a soft cloth, immersed in warm tap water to remove salt build-up.

  • Page 75: Checking Condition Of Lyse Tubing

    Abacus Service Manual Warning! Be careful, DO NOT twist the tube while reposition it into the head, because it will cause malfunction in a very short period of time. 8.2.7. Checking condition of lyse tubing Lyse reagent get the tubing material hard, at connections air can be aspirated to the system, which decreases volume of lyse reagent.

  • Page 76: Spare Parts

    Diatron MI PLC SPARE PARTS CODE NAME Abacus+ plastic front panel AP104 APX104 Front panel (Abacus+, excl. keyboard) AP132 Foil keyboard A133 HVB board with inverter (complete) AP136 LCD display A137 Lamp for LCD AP140 Amplifier board (Abacus+, complate) J156...
  • Page 77 Abacus Service Manual CODE NAME A402 Sealing ring for chamber A403 U-shaped metal fixing for apertures A404V2 Cone for WBC chamber A405 Shield for WBC chamber A406 Sealing ring for WBC chamber AP450 MIX chamber AP420 RBC chamber RBC aperture (80 µm) A421 A422 Ground electrode for RBC/WBC...
  • Page 78 Diatron MI PLC CODE NAME A550 Diluent cont. connector A551 Colour locking ring A552 Colour lock nut (inside) A553 Colour coding ring (outside) A554 Luer female A555 Luer male A556 2,3 mm T connector A557 Teflon regent cont. Connector J558...
  • Page 79 Abacus Service Manual CODE NAME AP7505 34P ribbon cable (amplifier board) AP7506 14P ribbon cable (microdilutor) AP7507 26P ribbon cable (dilutor) AP7508 3P ribbon cable (start button) AP7509 10P ribbon cable (HUR) AP132 Foil keyboard AP140 Amplifier board (complete) AP250 Dilutor block (complete) AP251 Lyse syringe with piston...

  • Page 80: Appendices

    Diatron MI PLC 10. APPENDICES 10.1. Warning flags In the followings we summarize the warning flags and give an explanation of their possible cause and a few hints to overcome the problem: Uppercase letters refer to WBC or HGB problems:...
  • Page 81 Abacus Service Manual Warning flags in lowercase refer to RBC or PLT problems: Meaning Flag Recommended user action PLT blank is high, Repeat the blank measurement and accept it. or no PLT blank Diluent problem. Replace diluent, open a new tank. RBC blank is high, Repeat the blank measurement and accept it.

  • Page 82: Serial Communication Protocol

    Diatron MI PLC 10.2. Serial Communication Protocol This document describes protocols 1.0, 1.7, 2.20, 2.23 and 3.0. Only protocol version 3.0 contains sending Limits for parameters. 10.2.1.General Description Abacus+ software is able to make serial connection link to a receiver device (computer) by connecting to the serial port of the instrument.

  • Page 83: Format Of Packages Sent

    Abacus Service Manual 10.2.2.Format of Packages Sent The packages sent by Abacus+ are always between a <SOH> and <EOT> character, and they consist of header, data and tail. The header consists of a package identifier and a package type descriptor. The tail includes the checksum. Typical format: <SOH>MID CMD<STX>MESSAGE<ETX>CHKSUM<EOT>...
  • Page 84 Diatron MI PLC DATA package This package contains sample and patient data, the measured parameters and the markers of histograms. One data element consists of two items: name and value, which are separated by a <HT> character, and closed by <LF>: SNO<HT>152<LF>...
  • Page 85 Abacus Service Manual The markers are given in histogram channel between 0 and 255, where 0 means that the marker could not be found. The parameter IDs in Abacus+ are: P01: WBC (10 /l); P02: RBC (10 /l); P03: HGB (g/l); P04: HCT (%);...
  • Page 86 Diatron MI PLC XML record descriptor package Each time a communication is initialized, Abacus+ will send an XML file in ASCII mode. A receiver application should be aware of this, by saving this XML file for later use. This package contains the description of the FULL record format. FULL record format means transmission of a record stored in Abacus+ as is, in binary format.
  • Page 87 Abacus Service Manual This field is an array, contains 23 entries, as indicated. Interpretation of these entries is not listed in the XML file. You will find them below: #define PAR_WBC #define PAR_RBC #define PAR_HGB // always transmitted in g/l unit #define PAR_HCT #define PAR_MCV #define PAR_MCH...

  • Page 88: Serial Protocol 1.0

    Diatron MI PLC 10.2.5.Serial Protocol 1.0 Receiver sends that it is ready to receive now. <ENQ> Abacus+ sends acknowledge. <ACK> Abacus+ sends INIT to initiate link (there is data to send): <SOH>AI<STX>Abacus+<HT>1.3<HT>20011005<HT>125027<ETX>30<EOT> Receiver sends acknowledge: <ACK><SPACE>A Abacus+ sends DATA package: <SOH>BD<STX>SNO<HT>152<LF>DATE<HT>19980715<LF>TIME<HT>114500<LF>...

  • Page 89: Serial Protocol 1.7

    Abacus Service Manual 10.2.6.Serial Protocol 1.7 Receiver sends that it is ready to receive now. <ENQ> Abacus+ sends acknowledge. <ACK> Abacus+ sends INIT next time to initiate link (there is data to send): <SOH>AI<STX>Abacus+<HT>2.22<HT>20011005<HT>135212<ETX>30<EOT> Receiver sends acknowledge: <ACK><SPACE>A Abacus+ sends DATA package: <SOH>BD<STX>SNO<HT>152<LF>DATE<HT>19980715<LF>TIME<HT>114500<LF>...

  • Page 90: Serial Protocol 2.20, 2.23

    Diatron MI PLC 10.2.7.Serial Protocol 2.20, 2.23 This version utilizes a bit upgraded header information: <SOH>BD<STX>SNO<HT>152<LF>DATE<HT>19980715<LF>TIME<HT>114500<LF> SID<HT>2<LF>PID<HT>26<LF>NAME<HT>JOE SMITH<LF>MODE<HT>0<LF>WRN<HT>0<LF> PM1<HT>12<LF>PM2<HT>204<LF>RM1<HT>51<LF>WM1<HT>23<LF>WM2<HT>57<LF> WM3<HT>92<LF>PARN<HT>22<LF>P01<HT> 6.6<HT>0<LF>P02<HT>4.29<HT>0<LF> P03<HT> 167<HT>0<LF> ... P21<HT> 8.2<HT>0<LF>P22<HT> 5.3<HT>0<LF> AGE<HT>24<LF><ETX>7C<EOT> A parameter called AGE is included in the header info. This represents the age of the given patient (if selected in service menu, and defined at sample info screen).

  • Page 91: Serial Protocol 3.0

    Abacus Service Manual 10.2.8.Serial Protocol 3.0 This version utilizes a new approach to record transmitting: It sends the INIT package, where instrument and PC should establish the communication. After connection is established, Abacus+ will send an „X‟ package with the XML file to give a description to the binary data sent in the „F‟...
  • Page 92 Diatron MI PLC Data map of TmeasData inside an F package Field name explanation note no. of bytes data type RecNo Internal Record ID WORD TimeStamp Timestamp of sample DateTime OperatorID Operator ID. Important in multiuser mode only. WORD SampleID...
  • Page 93 Abacus Service Manual Note 1: WORD data format is stored back to front. E.g. Decimal value 13553 Hexadecimal value 34 F1 Stored as: F1 34 Note 2: DateTime type is a packed binary format. It contains YEAR-MONTH-DAY-hour-minute in the following format: YYYYYYYYYYYY MMMM DDDDD hhhhh mmmmmm e.g.: 11/27/2005, 13:37 is stored as:...
  • Page 94 Diatron MI PLC Full scale and resolution of histograms: PLT – 50 fl, 1 fl x 50/256 RBC – 200 fl, 1 fl x 200/256 WBC – 400 fl, 1 fl x 400/256 Note 8: Flags for individual parameters. See page 4, remark 4 Note 9: Warning flags, see page 3, remark 1.

  • Page 95: Abacus + Cabling Diagram

    Abacus Service Manual 10.3. Abacus cabling diagram rev 1.09...

  • Page 96: Abacus+ Tubing Schematics

    Diatron MI PLC 10.4. Abacus+ tubing schematics Abacus + V 11 V 10 Csövezés Tubing schematics Version 1.4 2007.03. 14 A + Tubing . vsd PUFF Pump Left open rev 1.09...

  • Page 97: Recommended Kit Of Tools

    Abacus Service Manual 10.5. Recommended kit of tools PC standard keyboard (PS/2) Floppy drive with cables: A154+J727+J7505 Screwdrivers: Cross Slot Screwdrivers (Philips) Slot Screwdrivers Hexagon Screwdrivers (3.5, 2.5, 2.0, 1.5 mm sizes) Pocket digital multimeter Diagonal Cutter (plier) Nipper 10.6. Electronic schematics Electronic schematics can be found in the following Appendices (in PDF).

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