WPI UltraMicroPump II Instruction Manual page 13

Syringe Volume
Type
(defined for this length)
A 0.5 µl (54.1 mm) †
B 1.0 µl (54.1 mm) †
C** 5 µl (54.1 mm) †
D 10 µl (54.1 mm) †
E 25 µl (60.0 mm)
F 50 µl (60.0 mm)
G 100 µl (60.0 mm)
H 250 µl* (60.0 mm)
500 µl* (60.0 mm)
I
J 1000 µl* (60.0 mm)
K nanoliter 2000 † †
10 µl (60 mm)
L
M user defined
** ils005 (27 mm)
* Gas-tight syringes are not recommended for UMPII in these volumes; instead, use a liquid-tight
syringe to prevent drive motor damage.
** The ILS005 5 µL syringe must use type M with a 0.5880 nL / step entry. See page 15 for details.
† These are defined as 60 mm in Rev. H firmware.
† † WPI's Nanoliter 2000, a nanoliter injector for the 2-70 nL range, comes with its own simple controller
but may also be driven by the Micro4. For more information, enquire about WPI # B203XVY.
Note On Syringe Stroke Length
the delivery of the umPii is based on 60 mm or 54.1 mm syringes. Please note
which syringe length you are using as a factor of 0.9016 may need to be applied to
Maker Syringe
Hamilton 1700 series ,10 µl
Hamilton 700 series,10 µl
sGe 0.5 µl – 10 µl
ils 5 µl luer tip ils005
sGe 25 µl – 500 µl
WPi FlexiFil™
WPi nanoFil™
World Precision instruments
ID (mm)
0.1085 (0.102 reV H)
0.1534 (0.145 reVH)
0.343 (0.325 reV H)
0.485 (0.46 reV H)
0.73
1.03
1.46
2.3
3.26
4.61
0.48 plunger in 0.50 glass
0.4607 (0.100 rev H)
0.4856
Stroke
Length
60 mm
54.1 mm
54.1 mm
28 mm
60 mm
54.1 mm
60 mm
UltraMicroPump II
nL / step REV J
0.0294 ( 0.026 rev H)
0.0587 (0.052rev H)
0.2934 (0.263 rev H)
0.5868 (0.528 rev H)
1.329
2.646
5.315
13.191
26.501
52.995
2.3 nl /step (0.0005" step)
0.5293 (0.0249 rev H)
see page 12
0.5880 compensates for length as
tYPe m
the volume to be injected in or-
Use TYPE
der to have a precise injection.
For the ils005, use the tYPe
L
"m" values in the above table.
D
A,B,C,D
M
E - L
*Manufacturers have the right to
D
change this specification at any time.
L
Max. Rate
nl /sec or
nl /min
20
40
202
406
1022
2035
4088
9999
9999
9999
884
170
11