Acea NovoCyte Quick Start Manuals

NovoCyte and NovoCyte
®
STARTUP
1. Check for adequate levels of the NovoFlow
NovoClean™ in the fluidic containers. Ensure that each is at
least 1/2 full, and preferably empty waste container.
2. Press the power button on the front panel of the flow
cytometer to turn on the instrument. Launch the
NovoExpress software on the computer. Startup is
®
automated and takes approximately 6 minutes. Check the
instrument status as indicated by the LED indicator on the
front panel:
Green = Normal.
The instrument is ready to use.
Orange = Warning.
Click on the status bar in the NovoExpress software to review
the warning message.
Red = Error.
Click on the status bar to review the error message.
Refer to Section 5: Troubleshooting
Operator's Guide.
STARTUP
QC TEST
RUN
COMPENSATION
CONTROLS
(if applicable)
SETUP
SAMPLES AND PLOTS
VERIFY
CORRECT PARAMETERS AND
ACQUISITION SETTINGS
Events/Volume/Flow Rate/Threshold
RUN
SAMPLES
Quanteon Flow Cytometers
®
QUICK START & SAMPLE ACQUISITION GUIDE
, NovoRinse
®
in the Flow Cytometer
QC TEST
, and 1. Add 1 drop of the NovoCyte QC particles to 0.5 mL of dilution
®
buffer (0.4 mL PBS and 0.1 mL NovoRinse solution).
2. On the main menu, click on
Section → Click
complete the test.
3. If the QC Test results display Pass or Acceptable, the instru-
ment may be used to acquire data. If any QC Test results
display "Fail", refer to
Cytometer Operator's
FLUIDICS MANAGEMENT
1. The software will display a warning message instructing the
user to add more reagents when the volumes of NovoFlow,
NovoClean, and NovoRinse are less than the set thresholds of
0.9 L, 150 mL, and 150 mL, respectively.
2. To add reagents:
1) Make sure the instrument is Off or in the Ready status.
2) Disconnect the tubing from the container.
3) Bring the container to the sink. Unscrew the cap and add
more reagent to the container.
4) Screw the cap back on, reconnect the quick coupler, and
return the container back to the fluidics station. Ensure
that the tubing is not twisted or kinked.
5) If the instrument was in the Ready status, click
tab → Under
software to prime the system before running samples.
3. Similarly, the software will warn the user to empty the waste
container when the waste volume exceeds 2.8 L.
1) Follow the steps above to disconnect the waste container
from the instrument. Dispose of the contents according to
local safety guidelines.
2) Add 300 mL of bleach to the waste container and
reconnect the container to the instrument.
SHUTDOWN
1. Press the power button on the front panel of the flow
cytometer. The instrument will automatically perform the
shutdown cleaning process and then power off.
2. When completed the LED indicator will turn off.
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Instrument tab → Under
QC Test and follow the software prompts to
Section 5: Troubleshooting
Guide.
Fluidics Maintenance → Click Priming
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Operation
in the Flow
Instrument
in the