Acea RTCA SP Instrument Operator's Manual
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Acea RTCA SP Instrument Operator's Manual

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RTCA SP Instrument
Operator's Manual
Version January 2013

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Summary of Contents for Acea RTCA SP Instrument

  • Page 1 RTCA SP Instrument Operator’s Manual Version January 2013...

  • Page 3: Table Of Contents

    RTCA Cleaning and Replacement Equipment ..................27 Installation ................................. 28 Installation Warnings ............................28 Unpacking ................................28 Space and Power Requirements ........................29 Environmental Requirements ........................29 Installation of the RTCA SP Instrument ....................29 Resistor Plate Verifi cation of the RTCA SP Instrument ................31...
  • Page 4 I started an experiment. There was an error message, “ACK communication lost. ” What happened? ..................54 How do I reinitialize the RTCA SP Instrument after a power failure? ..........55 The Cell Index signal is signifi cantly lower than usual. Why? ............55 The Cell Index signal decreases with time, but there was no compound added.

  • Page 5: Prologue

    November 2009 January 2013 © Copyright 2013, ACEA Biosciences, Inc. All rights reserved. Information in this docu- ment is subject to change without notice. No part of this document may be reproduced or transmitted in any form or by any means, electronic or mechanical, for any purpose, without the express written permission of ACEA Biosciences, Inc..

  • Page 6: Declaration Of Conformity

    ACEA Biosciences. It can be used for both high throughput screening and research laboratory environments. e RTCA SP Instrument is intended for life science research and must be used exclusively by laboratory professionals who are trained in laboratory techniques and have studied the instructions for use of this instrument.

  • Page 7: License Statements For The Instrument

    Please comply with all statements made in these documents. Preamble e RTCA SP Instrument Operator’s Manual has to be used in conjunction with the corresponding RTCA So ware Manual. Before setting-up operation of the RTCA SP Instrument, it is important to read this Operator’s Manual and the corresponding RTCA So ware Manual thoroughly and completely.

  • Page 8: Use Of The Instrument Operator's Manual

    Use of the Instrument Operator’s Manual Use of the Instrument Operator’s Manual is Operator’s Manual describes the operation of the RTCA SP Instrument and contains the following chapters: Chapter A Overview contains a short introduction to the operation of the RTCA SP In- strument and describes the instrument’s speci cations.
  • Page 9 Conventions Used in this Manual Symbols In this Operator’s Manual symbols are used as a visual signal to highlight important points. Symbol Heading Description WARNING This symbol is used to indicate that noncompliance with instructions or procedures could lead to physical injury or even death or could cause damage to the instrument.

  • Page 10: Warnings And Precautions

    Warnings and Precautions Handling Requirements e RTCA SP Instrument must only be used by trained and skilled personnel. It is essential that the following safety information required for installation and operation of the RTCA SP Instrument is carefully read and observed. Please ensure that this safety information is accessible to every employee working with the RTCA SP Instrument.
  • Page 11 fi re. Keep the cover closed while the instrument is connected to the mains and do not use sprays in the vicinity of the RTCA SP Instrument. During fi refi ghting operations, disconnect the RTCA SP Instrument from the mains.

  • Page 12: Xiii. Disposal Of The Instrument

    For more information about disposal of your old product, please contact your For more information about disposal of your old product, please contact your city offi ce, waste disposal service or your local ACEA Support personnel. city offi ce, waste disposal service or your local ACEA Support personnel.

  • Page 13: Overview

    Over 95% of current biological assays are based on optical detection (e.g., uorescent and optical detection in a uorescence plate reader, optical microscopy, FACS analyzer, uorescence scanner). In contrast, the new RTCA SP Instrument developed by ACEA Biosciences is based on electronic detection of biological assay processes, thereby integrating molecular and cell biology with microelectronics.

  • Page 14: Rtca Sp Instrument

    Furthermore, the impedance change also depends on the extent to which cells attach to the electrodes (Figure 1). For example, cell spreading resulting in a large cell/electrode contact area leads to a large change in impedance. Figure 1: Schematic illustration of the impedance measurement principle. RTCA SP Instrument Operator’s Manual...
  • Page 15 RTCA SP Instrument consists of the following main components: RTCA Control Unit RTCA Analyzer E-Plate 96 RTCA SP Station Figure 2: The RTCA SP Instrument with the main components: RTCA Analyzer, RTCA SP Station, E-Plate 96 and RTCA Control Unit. Overview...
  • Page 16 RTCA So ware can be used for simpli ed experimental design and set-up; it will then direct the instrument to perform the experiment and provide fully automated data acquisition, data analysis, and data presentation. RTCA SP Instrument Operator’s Manual...

  • Page 17: Specifi Cations Of The Instrument

    Speci cations of the Instrument Specifi cations of the RTCA Analyzer Specifi cations of the Instrument e RTCA SP Instrument has to be operated at an altitude/pressure of 0 –2000 m above sea level, 80 –106 kPa. Specifi cations of the RTCA Analyzer ...

  • Page 18: Specifi Cations Of The E-Plate 96

    RTCA Resistor Plate 96 are di erent variables than raw scan data acquired during resistor plate veri cation. erefore, they are not comparable to acceptance values listed in chapter Resistor Plate Veri cation of the RTCA SP Instrument. RTCA SP Instrument Operator’s Manual...

  • Page 19: B System Description

    System Package System Description System Package e table below lists all the components required for the RTCA SP Instrument. Check for completeness and inspect packaging prior to installation. e original shipping boxes must be transferred unopened to the installation site.

  • Page 20: System Description

    96-well microplate devices. is permits the RTCA SP Instrument to measure the activity of living cells in real-time without any labels or reporters. Any number of cellular parameters such as attachment, spreading, growth, death, and even speci c morphological changes can be simply and reliably detected with the RTCA SP Instrument.

  • Page 21: Description Of The Rtca Analyzer

    System Description Description of the RTCA Analyzer Description of the RTCA Analyzer e RTCA Analyzer is an electronic analyzer that can, under the control of RTCA So ware, conduct electronic impedance measurement of sensor electrodes at vari- ous signal frequencies. e RTCA Analyzer is capable of computer-controlled signal generation, processing and analysis, automatic frequency scanning and rapid measure- ment.

  • Page 22: Description Of The Rtca Sp Station

    Contact Pins 96, each for connecting to one of the 120 conductive pads on the back of an E-Plate 96. Cradle Pocket Clamp Plate RTCA Protector Shield 96 RTCA Contact Pins 96 Lock Handle Status LEDs Figure 7: The RTCA SP Station. RTCA SP Instrument Operator’s Manual...

  • Page 23: Cradle Pocket And Clamp Plate

    System Description Description of the RTCA SP Station 2.2.1 Cradle Pocket and Clamp Plate e cradle pocket and the clamp plate of the RTCA SP Station provide mechanical support and electrical connection from the E-Plates 96 to the electronic circuits of the station. cradle pocket assembly connects the sensors in each well of the E-Plate 96 to the PCB board through the RTCA Contact Pins 96.

  • Page 24: Rtca Control Unit

    RTCA Control Unit e RTCA Control Unit consists of a laptop computer (Figure 9). e operating system and all so ware tools necessary to run the RTCA SP Instrument are already pre-installed. Figure 9: The RTCA Control Unit. RTCA Control Unit Cat.

  • Page 25: E-Plate 96 And Rtca Resistor Plate 96

    E-Plate 96 and RTCA Resistor Plate 96 E-Plates 96 are single use, disposable devices used for performing cell-based assays on the RTCA SP Instrument. e E-Plate 96 is similar to commonly used 96x well micro- titer plates. Each well of the 96 wells on the E-Plates 96 has incorporated sensor electrode arrays so that cells inside each well can be monitored and assayed.

  • Page 26: Rtca Frame 96

    RTCA Resistor Plate 96 Cat. No. 05232350001 RTCA Frame 96 Cat. No. 05232392001 6 Units Cat. No. 05232368001 E-Plate 96 6 × 6 Units Cat. No. 05232376001 RTCA Contact Pins 96 (20 Units) Cat. No. 05232384001 RTCA SP Instrument Operator’s Manual...

  • Page 27: Rtca Software

    System Description RTCA Software RTCA Software e RTCA So ware provides unparalleled instrument control for exible experiment setups, data acquisition, and data analysis. All instrument and experiment controls are embedded in the RTCA So ware to simplify instrument setup and operation. So ware is used with the RTCA Analyzer in conjunction with a single RTCA SP Station.

  • Page 28: Installation

    RTCA SP Instrument. While a stand-alone RTCA SP Instrument can work with a two-pronged AC Instrument. While a stand-alone RTCA SP Instrument can work with a two-pronged AC...

  • Page 29: Space And Power Requirements

    Do not place the RTCA Analyzer on top of a cell culture incubator. Please avoid to install the RTCA SP Instrument in direct vicinity of other laboratory Please avoid to install the RTCA SP Instrument in direct vicinity of other laboratory...
  • Page 30 Ideally, before placing the RTCA SP Station inside an incubator, it is recommended to set up the RTCA SP Instrument outside the incubator using a procedure similar to set up the RTCA SP Instrument outside the incubator using a procedure similar to that being described here in section 3.5, and to perform a Resistor Plate Verifi...

  • Page 31: Resistor Plate Verifi Cation Of The Rtca Sp Instrument

    Resistor Plate Verifi cation of RTCA SP Instrument Resistor Plate Verifi cation of RTCA SP Instrument Resistor Plate Verifi cation of the RTCA SP Instrument Resistor Plate Verifi cation of the RTCA SP Instrument Resistor Plate Veri cation is a very important step to verify the installation and the Resistor Plate Veri cation is a very important step to verify the installation and the functionality of the RTCA SP Instrument.
  • Page 32 Installation Installation Resistor Plate Verifi cation of RTCA SP Instrument Resistor Plate Verifi cation of RTCA SP Instrument Place the RTCA Resistor Plate 96 in the RTCA SP Station Place the RTCA Resistor Plate 96 in the RTCA SP Station Lift the clamp plate of the RTCA SP Station.
  • Page 33 Installation Installation Resistor Plate Verifi cation of RTCA SP Instrument Resistor Plate Verifi cation of RTCA SP Instrument Lift the lock handle of the RTCA SP Station, so that the two bearings of the clamp plate Lift the lock handle of the RTCA SP Station, so that the two bearings of the clamp plate fi...
  • Page 34 If the acceptance criteria described in 5. and 6. are fulfi lled, the resistor plate verifi cation is passed successfully. If the criteria are not fulfi lled, please contact your local ACEA is passed successfully. If the criteria are not fulfi lled, please contact your local ACEA representative.

  • Page 35: C Operation

    Introduction Introduction Operation Operation Introduction Introduction Prior to the operation of the RTCA SP Instrument, please refer to the Prior to the operation of the RTCA SP Instrument, please refer to the Overview Overview Overview and Overview Sys- Sys- tem Description...

  • Page 36: Preparing And Starting A Run On The Rtca Sp Instrument

    Preparing and Starting a Run on the RTCA SP Instrument Preparing and Starting a Run on the RTCA SP Instrument Running a Quick Experiment Running a Quick Experiment Preparing and Starting a Run on the RTCA Preparing and Starting a Run on the RTCA...
  • Page 37 Preparing and Starting a Run on the RTCA SP Instrument Preparing and Starting a Run on the RTCA SP Instrument Running a Quick Experiment Running a Quick Experiment Insert the E-Plate 96 Insert the E-Plate 96 1. Insert the E-Plate 96 front end (with corner cut) into the cradle pocket of the RTCA 1.
  • Page 38 Preparing and Starting a Run on the RTCA SP Instrument Preparing and Starting a Run on the RTCA SP Instrument Running a Quick Experiment Running a Quick Experiment Set up the Layout page Set up the Layout page Layout page...
  • Page 39 After completing all of the sweeps within a step, the RTCA SP Instrument does not After completing all of the sweeps within a step, the RTCA SP Instrument does not move on to the next step until it is manually instructed to do so.
  • Page 40 Preparing and Starting a Run on the RTCA SP Instrument Preparing and Starting a Run on the RTCA SP Instrument Running a Quick Experiment Running a Quick Experiment   Step 3 typically comes after an action (for example, compound addition) on the...
  • Page 41 Preparing and Starting a Run on the RTCA SP Instrument Preparing and Starting a Run on the RTCA SP Instrument Running a Quick Experiment Running a Quick Experiment Remove the E-Plate 96 and add cells to the E-Plate 96 wells...

  • Page 42: Important Tips For Using The Rtca Sp Instrument

    In order to obtain optimal data and to avoid any problem with the RTCA SP Instrument, In order to obtain optimal data and to avoid any problem with the RTCA SP Instrument, it is essential that you observe the following instrument handling procedures: it is essential that you observe the following instrument handling procedures: Always perform background measurement of E-Plate 96 with cell-free culture media.
  • Page 43 If cell number is too high or too low, it would a ect the data quality generated from the RTCA SP Instrument. too low, it would a ect the data quality generated from the RTCA SP Instrument.

  • Page 44: D Maintenance And Care

    RTCA SP Station to be placed inside an incubator. Avoid spraying any sterilizing agent directly on any part of the RTCA SP Instrument, Avoid spraying any sterilizing agent directly on any part of the RTCA SP Instrument,...

  • Page 45: Cleaning Instructions

    General Cleaning General Cleaning General cleaning of the RTCA SP Instrument is simple. Use some so cloth or ber free General cleaning of the RTCA SP Instrument is simple. Use some so cloth or ber free tissue paper moistened slightly with 80% alcohol, gently wipe o any dust or contaminants tissue paper moistened slightly with 80% alcohol, gently wipe o any dust or contaminants from the surface of the RTCA SP Station.

  • Page 46: Cleaning And Exchanging The Rtca Contact Pins 96

    When using blower to remove dust, always remember to blow the air towards the When using blower to remove dust, always remember to blow the air towards the outside of the cradle area. outside of the cradle area.   RTCA SP Instrument Operator’s Manual RTCA SP Instrument Operator’s Manual...
  • Page 47 Cleaning and Exchanging the RTCA Contact Pins 96 Cleaning and Exchanging the RTCA Contact Pins 96 Repeat the above procedure to clean the other row of Contact Pins. Repeat the above procedure to clean the other row of Contact Pins. Figure 28: Using the Nylon Brush to clean the RTCA Contact Pins 96 in only one direction at a Figure 28: Using the Nylon Brush to clean the RTCA Contact Pins 96 in only one direction at a time (up to down in the Figure).
  • Page 48 Spare RTCA Contact Pins 96 are included with RTCA SP Instrument. If more RTCA Spare RTCA Contact Pins 96 are included with RTCA SP Instrument. If more RTCA Contact Pins 96 are needed, please contact your local ACEA representative.
  • Page 49 Cleaning and Exchanging the RTCA Contact Pins 96 Cleaning and Exchanging the RTCA Contact Pins 96 For example, if well G10 would have a contact problem, the RTCA Contact Pin for G10 For example, if well G10 would have a contact problem, the RTCA Contact Pin for G10 and/or the corresponding Pin for ground (GND) would have to be exchanged.

  • Page 50: Exchanging Fuses

    If the new fuse gets burned again, please unplug the RTCA steps to replace the fuse. If the new fuse gets burned again, please unplug the RTCA Analyzer power and contact your local ACEA representative. Analyzer power and contact your local ACEA representative.
  • Page 51 Exchanging Fuses Exchanging Fuses Remove the old fuses from the drawer. Remove the old fuses from the drawer. Insert new fuses as shown. Insert new fuses as shown. Figure 35: Inserting new fuses in the drawer. Figure 35: Inserting new fuses in the drawer. Press the fuse drawer, along with the fuses, into the AC Receptacle.

  • Page 52: E Appendix

    Disconnect the fl at cable from the RTCA Analyzer and from the RTCA SP Station. Reconnect the fl at cable. Reconnect the fl at cable. Turn on the RTCA Analyzer. Turn on the RTCA Analyzer. Click Retry. Click Retry. Retry. Retry   RTCA SP Instrument Operator’s Manual RTCA SP Instrument Operator’s Manual...

  • Page 53: I Started An Experiment And There Was A Sound And Error Message, "No Station", What Happened

    Troubleshooting Troubleshooting I started an experiment and there was a sound and error message, “No Station”, what happened? I started an experiment and there was a sound and error message, “No Station”, what happened? I started an experiment and there was a sound and error I started an experiment and there was a sound and error message, “No Station”, what happened? message, “No Station”, what happened?

  • Page 54: I Started An Experiment. There Was An Error Message, "Ack Communication Lost." What Happened

    Click Continue button in the top left corner of the RTCA Software. Click Continue button in the top left corner of the RTCA Software. Continue button in the top left corner of the RTCA Software. Continue   RTCA SP Instrument Operator’s Manual RTCA SP Instrument Operator’s Manual...

  • Page 55: How Do I Reinitialize The Rtca Sp Instrument After A Power Failure

    Troubleshooting Troubleshooting How do I reinitialize the RTCA SP Instrument after a power failure? How do I reinitialize the RTCA SP Instrument after a power failure? How do I reinitialize the RTCA SP Instrument after a How do I reinitialize the RTCA SP Instrument after a...

  • Page 56: The Cell Index Signal Decreases With Time, But There Was No Compound Added. Why

    Continue the experiment a er putting the E-Plate 96 back in. en Continue the experiment a er putting the E-Plate 96 back in. the experiment a er putting the E-Plate 96 back in. RTCA SP Instrument Operator’s Manual RTCA SP Instrument Operator’s Manual...

  • Page 57: I Did A Background Measurement. The Rtca Software Indicated That Many Wells May Have Background Problems. What Should I Do

    96 for another Scan Plate test. If the number of open-circuit wells is the same as before, 96 for another Scan Plate test. If the number of open-circuit wells is the same as before, then please report the problems to your local ACEA representative. then please report the problems to your local ACEA representative.

  • Page 58: The Device Indicating Led Did Not Turn Green. What Should I Do

    Check whether the Device Indicating LED turns green. If the LED did not turn green (no light), then please contact your local ACEA representative. the LED did not turn green (no light), then please contact your local ACEA representative.

  • Page 59: Ordering Information

    ACEA Biosciences Service and Support ACEA Biosciences Service and Support At ACEA, we are committed to providing innovative, high-quality products and excellent At ACEA, we are committed to providing innovative, high-quality products and excellent customer service. For a complete overview of our products or to nd a local representa- customer service.
  • Page 60 6779 Mesa Ridge Rd. , Suite 100 6779 Mesa Ridge Rd. , Suite 100 San Diego, CA 92121 San Diego, CA 92121 www.aceabio.com www.aceabio.com 2013 ACEA Biosciences, Inc. 2013 ACEA Biosciences, Inc. © © All rights reserved. All rights reserved.

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