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Acea NovoCyte Maintenance Manual

Flow cytometer
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NovoCyte
Flow Cytometer
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Maintenance Guide
V2

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Summary of Contents for Acea NovoCyte

  • Page 1 NovoCyte Flow Cytometer ® Maintenance Guide...

  • Page 3: Table Of Contents

    Table of Content Prologue Revision History ........................5 Contact Information .........................6 III. Using this Guide ........................6 Conventions ..........................6 Technical Support ........................7 Scheduled Maintenance Preventative Maintenance ......................9 Replacing Fluidic System Consumables ................13 Unscheduled Maintenance Remove Bubbles ........................19 Unclog the Sample Injection Probe (SIP) ................ 20 Clean the External Surface of the Instrument ..............
  • Page 4 Table of Content...

  • Page 5: Prologue

    © ACEA Biosciences, Inc. ACEA NovoExpress software is the property of ACEA Biosciences, Inc. Each sale of a stored unit of this soft- ware grants the purchaser a nonexclusive personal license. This software may not be duplicated, reproduced, or copied in any form or by any means whatsoever, except as otherwise permitted by law.

  • Page 6: Contact Information

    III. Using this Guide This maintenance guide describes the maintenance of the ACEA NovoCyte flow cytom- eter. Most of these functions are controlled by the ACEA NovoExpress software. For ad- ® ditional information about the ACEA NovoCyte, please refer to...

  • Page 7: Technical Support

    Details of recent instrument QC test. For support within the US, please call 866-308-2232. For support within China, please call 400-600-1063. For users in other countries or regions, contact your local ACEA Biosciences representa- tives or distributors, which may be found at www.aceabio.com.

  • Page 8: Scheduled Maintenance

    This chapter describes the procedures to execute or con- duct the maintenance procedures. Table 1-1 lists the regular maintenance to be done on the NovoCyte instrument. Maintenance Recommended Maintenance Frequency Daily Start-up Daily.

  • Page 9: Preventative Maintenance

    ► Clean the Sample Injection Probe (SIP) Materials ► Clean soft cloth ► ACEA NovoClean solution (1X) ► Deionized (DI) water Procedure Turn off the power of the instrument. Open the top cover of the instrument. Locate the SIP.
  • Page 10 Scheduled Maintenance Preventative Maintenance Unscrew the brown connector by turning it counter clockwise. Figure 1-2 Unscrew the Brown Connector Sample injection probe directly contacts the biological samples which may be biohazardous. Wear gloves when handling the sample injection probe. Pay attention to the ferrule (indicated with a red arrow in Figure 1-3) at the end of the tubing after removing the brown connector from the slot.
  • Page 11 When cleaning the sample injection probe, please be careful and prevent deform- ing or bending it. Prepare a damp cloth with ACEA NovoClean solution (1X). Wipe the sample injection probe’s surface back and forth until the surface is cleaned. Prepare a damp cloth with DI water. Wipe the sample injection probe back and forth for at least five times to clean the ACEA NovoClean solution residue.
  • Page 12 ► Unclog the Flow Cell When the NovoCyte is in Ready status, click Instrument → Fluidics Maintenance → Un- clog from NovoExpress software, and the Unclog procedure will automatically be execut- ed. The progress of the procedure will be shown in the NovoExpress status bar.

  • Page 13: Replacing Fluidic System Consumables

    Wipe the outer surface of the container using medical gauze pads. Empty the reagent container. Fill the reagent container with at least 300 mL of ACEA NovoClean solution (1X) and cap the bottle. Shake the container vigorously to let the ACEA NovoClean solution cover the entire inner surface.
  • Page 14 Figure 1-7 NovoExpress Software Message for Fluidics Consumables Replacement The following fluidic system consumables should be replaced at the same time: ► ACEA NovoFlow sheath fluid in-line filter (1pcs) (Cat. No. 2030002) ► Waste filter (1pcs) (Cat. No. 2030003) ►...
  • Page 15 Maintenance → Priming on the NovoExpress software when the instrument is in Ready status. Once the Priming procedure is completed, a dialog box will be prompted for conduting the QC Test (Figure 1-12). Prepare the NovoCyte QC particle sample as described in Sec- ®...
  • Page 16 Priming process by clicking Instrument → Fluidics Maintenance → Priming. ► Replace the ACEA NovoFlow Sheath Fluid In-Line Filter Open the front panel of the NovoCyte instrument, and locate the sheath fluid in-line filter shown in Figure 1-13. Figure 1-13 Sheath Fluid In-line Filter Procedure Turn off the power of the instrument.
  • Page 17 Scheduled Maintenance Replacing Fluidic System Consumables Replace the Waste Filter ► Open the side panel of the NovoCyte instrument, and locate the waste filter as shown in Figure 1-14. Figure 1-14 Waste Filter Procedure Turn off the power of the instrument.
  • Page 18 Procedure Make sure the NovoCyte is in Ready status. Press the metal clip on the quick coupler to disconnect the tubing from the ACEA Novo- Flow sheath fluid container. Unscrew the cap and take the tubing-filter assembly out from the container and put it on a clean lint-free wipe (Figure 1-15).

  • Page 19: Unscheduled Maintenance

    In this case, the following procedure should be conducted to remove the bubbles. When the NovoCyte instrument is in Ready status, place a tube containing at least 1 mL of 70% ethanol into the sample holder. Click Instrument → Fluidics Maintenance → De-...

  • Page 20: Unclog The Sample Injection Probe (Sip)

    During a test, if much fewer events are detected than expected, there is possibility that the SIP is clogged. To solve this issue, when the NovoCyte instrument is in Ready status, click Instrument → Fluidics Maintenance → Backflush to execute the Backflush procedure to unclog the SIP.

  • Page 21: Clean The External Surface Of The Instrument

    Figure 2-2 Connect the SIP with a 10 mL Syringe Immerse the other end of the SIP into a beaker containing ACEA NovoClean solution (1X). Push the plunger of the syringe back and forth 10 times to flush the SIP with ACEA NovoClean solution.

  • Page 22: Clean The Sample Injection Probe (Sip)

    If the outer surface of the sample injection probe is dirty, follow the procedure below to manual clean the outer surface of the sample injection probe. Materials ► Clean soft cloth ► ACEA NovoClean solution (1X) ► Deionized (DI) water Procedure Click Instrument → Shut Down on the NovoExpress software.
  • Page 23 After clicking the OK button, the sample injection probe will move downwards. Prepare a damp soft cloth with ACEA NovoClean solution (1X) and gently wipe the outer surface of the sample injection probe.

  • Page 24: Decontamination

    Unscheduled Maintenance Decontamination Please put a tube of filtered DI water or ACEA NovoRinse solution (1X) in the sample holder and click OK button again ( Figure 2-6). The instrument will shut down automati- cally. Figure 2-6 Message in NovoExpress Software to Conduct the SIP Cleaning Procedure...

  • Page 25: Clean Fluidic System Before Long-Term Storage

    Clean Fluidic System before Long-Term Storage If there is no plan to run the NovoCyte flow cytometer for more than two weeks, a thor- ough cleaning process is recommended to prevent salt crystallization in the fluidic system.

  • Page 26: Purge Fluidic System Before Shipment

    The NovoCyte instrument is then ready to use. If NovoCyte instrument has not been used for more than two months, it is recommend- ed to replace the fluidic system consumables before use. Refer to 1.2 Replace Fluidic System Consumables for detailed procedure.

  • Page 27: Calibrate The Fluidics Station

    Fluidics station is calibrated at the factory and should be ready to use when connected to NovoCyte instrument after installation. However, in some cases there is a need to recali- brate the fluidics station after shipment and movement. When a false warning about the liquid level in the reagent containers is observed, follow the procedures below to reca- librate the fluidics station.

  • Page 28: Clean The Optical Filters And Mirrors

    Unscheduled Maintenance Clean the Optical Filters and Mirrors Clean the Optical Filters and Mirrors In normal operation, the optical filters and mirrors are tightly enclosed inside the optical fixtures and should remain clean. However, it might get dirty if unplugged from the slot or be replaced.
  • Page 30 Published by ACEA Biosciences, Inc. 6779 Mesa Ridge Road Ste 100, San Diego, CA 92121 U.S.A. (866) 308-2232 www.aceabio.com © 2016 ACEA Biosciences, Inc. All rights reserved. ID150058...

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