Biocontrol LIGHTNING MVP ICON Manual

Surface Sampling Device
GENERAL DESCRIPTION
The LIGHTNING MVP ICON Surface Sampling Device is a self‐
contained device for use with the LIGHTNING MVP ICON™ or
®
LIGHTNING MVP systems for quality and HACCP‐related
monitoring of the environment. The MVP ICON and
LIGHTNING MVP systems detect food and microbiological
residues by measuring Adenosine triphosphate (ATP) using
luciferase bioluminescence. ATP is found in most food
residues and all bacteria, yeast and mold cells.
Part No. 64003‐100 (100 tests)
KIT COMPONENTS
One box of 100 surface sampling devices
IMPORTANT USAGE GUIDELINES
It is critical to insert the sampling device
into the MVP ICON with the two flat
sides of the sampling device's base
chamber facing left and right. Improper
sampling device orientation may result in
damage to the instrument.
Do not insert wet or contaminated
sampling devices into the instrument. Wipe condensate from
exterior of device before use with a clean, lint‐free cloth.
Avoid handling the device with wet or contaminated hands.
Always remove sampling devices from instrument after
reading.
Do not store the MVP ICON or MVP instrument on its side or
back. Avoid storing in humid areas where water leaks are
possible.
TEST PROCEDURE
A. Collecting Sample
(a) Twist to remove sampling device from body tube. The
swab tip must be moist. If fully saturated, any excess
wetting agent should be blotted off by gently pressing
the swab tip against the interior of the body tube. Be
careful not to touch the swab or inside the sampling
device with fingers.
(b) Thoroughly swab a standard 10 x 10 cm (4 x 4 in) area of
interest for a typical flat surface. For irregularly shaped
surfaces, ensure swabbing technique remains consistent
each time you swab. Reinsert the swab into the body
tube using a twisting motion to ensure it is sealed.
Note: After swabbing, the sampling device is stable for one
hour prior to activation.
Flat Side
B. Activating Device and Reading Results
(a) To activate the sampling device, hold upright and push
the plunger down until it is fully depressed. The reagents
will be dispensed through the sampling device washing
the sample off the swab tip and into the collection tube.
(b) Insert the sampling device into the MVP ICON or MVP
and read the results. When inserting into the MVP ICON,
ensure the flat sides of the sampling device base
chamber face left and right in the instrument.
Note: Results should be read within two minutes of
activation. Please refer to the MVP ICON User Guide or MVP
System Guide for operating instructions.
INTERPRETING RESULTS
(a) When using default thresholds in the MVP ICON or MVP
systems, readings of 2.5 Zones or less indicate the
surface is considered clean.
(b) Readings between 2.6 ‐ 3.0 Zones indicate a warning the
surface is not adequately clean.
(c) If the reading is greater than 3.0 Zones, the surface is
considered dirty and should be re‐cleaned and re‐tested.
CONTROLS
A. Positive Control
It is recommended LIGHTNING MVP ICON ATP Positive
Controls (Part No. 64001‐25) be run every other day to verify
integrity of the reagent system.
(a) A positive control should yield a result from 3.1 to 4.3
Zones. See ATP Positive Control Directions for Use for
additional information.
B. Negative Control
A negative control is an unused yet activated LIGHTNING MVP
ICON Surface Sampling Device.
(a) Negative control results should read between 1.0 to 2.5
Zones.
(b) Readings above 2.5 may indicate contamination of the
MVP ICON or MVP sampling chamber or sampling device.
See ATP Positive Control Directions for Use for additional
information. Contact BioControl Technical Support at
800.245.0113 for more information.
PRECAUTIONS
The appearance of a yellow band on the swab tip is normal
and will not affect performance.
If accidental activation prior to sampling occurs, do not use
device.