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Baker Invivo2 UM-014 User Manual

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UM-014
Invivo
Plus User Manual
2
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Summary of Contents for Baker Invivo2 UM-014

  • Page 1 UM-014 Invivo Plus User Manual Affix Serial Number Sticker Here...

  • Page 2: Product Summary

    Invivo Plus Product Summary Baker Ruskinn's Invivo hypoxia workstation allows you to isolate cells in a chamber that accurately maintains and controls temperature, humidity, oxygen and carbon dioxide. With the Invivo , you can study even the most complex cell interactions under perfect microaerophilic or anaerobic conditions.

  • Page 3: Table Of Contents

    Invivo Plus Table of Contents Product Summary ........................... 2 Features and Benefits ..........................2 Overview and Safety Instructions ....................6 Regulatory compliance ....................7 Symbols ......................... 7 Installation and relocation .................... 8 Weight and dimensions ....................9 Gas and electrical supply requirements .................. 10 Gas supply requirements ....................
  • Page 4 Invivo Plus 4.2.1 Standard humidity control ................23 Environmental gas composition control .................. 23 Using the workstation chamber....................25 Using the interlock ...................... 25 6.1.1 Interlock overview ..................25 6.1.2 Opening the interlock outer door ..............25 6.1.3 Opening the interlock inner door ..............25 6.1.4 Transferring material into the workstation chamber via the interlock ..
  • Page 5 Invivo Plus 7.4.2 Replacing an Ezee Sleeve ................44 7.4.3 Replacing detox sachets ................. 44 7.4.4 Installing/ replacing a catalyst sachet ............. 46 7.4.5 Replacing the mains plug fuse – UK users only ..........48 7.4.6 Replacing the mains fuses ................49 Service requirements ....................

  • Page 6: Overview And Safety Instructions

    Invivo Plus Overview and Safety Instructions Please read this manual carefully to familiarise yourself with the operation and maintenance of your Invivo Plus workstation. Note: The workstation should be located in a well-ventilated area. For your safety, safety of others using the workstation and those around you; •...

  • Page 7: Regulatory Compliance

    Invivo Plus Regulatory compliance This product complies with the essential EEA requirements for Electrical Safety and the Low Voltage Directive 2006/95/EC as well as Electromagnetic compatibility as set out in the EMC Directive 204/108/EC. Symbols Before using the Invivo Plus, please ensure that you are familiar with the symbols on the Invivo Plus.

  • Page 8: Installation And Relocation

    Invivo Plus Warning, this equipment contains high voltage circuitry. Warning: Do not use toxic or Flammable substances inside the incubator. Invivo Plus contains hazardous components and must not be disposed of at a household waste site. Instead it should be taken to the appropriate collection point for the recycling of electrical and electronic equipment.

  • Page 9: Weight And Dimensions

    Invivo Plus Weight and dimensions The Invivo Plus workstations weigh approximately 130kg. Figure 2 lists the dimensions of the Invivo Plus workstation; Figure 2: Invivo2 Plus workstation dimensions External width 1586 mm External height 725 mm External depth 714 mm Workstation chamber internal width 1050 mm Workstation chamber internal height...

  • Page 10: Gas And Electrical Supply Requirements

    Invivo Plus Gas and electrical supply requirements Gas supply requirements The Invivo Plus workstation requires; • Oxygen free nitrogen. • In addition to this, the Invivo Plus workstation should be connected to ICO IC (supplied with the Invivo Plus workstation). All other gas connections should be made to the See UM-011 ICO IC user manual for more details.

  • Page 11: Powering The Workstation

    Invivo Plus Note: The power consumption is for normal operating conditions with no equipment connected to the internal power supply. Power consumption will vary dependent upon the conditions inside the workstation chamber and the conditions inside the room the workstation is located in. Powering the workstation To switch the workstation on, press the power switch (item 6 in Figure 15).

  • Page 12: Workstation Overview

    Invivo Plus Workstation overview Workstation layout The Invivo Plus workstation consists of 2 main areas; the workstation chamber and the interlock chamber. The workstation chamber is the main working area of the workstation. Access to the workstation chamber is provided by the glove ports, the Single Plate Entry System (SPES) and the interlock.

  • Page 13: Rear View

    Invivo Plus 3.2.2 Rear view Figure 5 shows the rear view of the Invivo Plus workstation; Figure 5: Invivo2 Plus workstation rear view 1. Cooling vent outlet. UM-14 Version R3.0 Page 13 Invivo Plus User Manual...

  • Page 14: Left Side View - Standard Humidifier

    Invivo Plus 3.2.3 Left side view – Standard humidifier Figure 6 shows the left side view of the Invivo Plus workstation with the standard humidifier; Figure 6: Invivo2 Plus workstation left side view with standard humidifier 1. Exhaust valve outlet. 2.

  • Page 15: Left Side View - Ultrasonic Humidifier

    Invivo Plus 3.2.4 Left side view – Ultrasonic humidifier Figure 7 shows the left side view of the Invivo Plus workstation with the ultrasonic humidifier; Figure 7: Invivo Plus workstation left side view with ultrasonic humidifier 1. Exhaust valve outlet. 2.

  • Page 16: Right Side View

    Invivo Plus 3.2.5 Right side view Figure 8 shows the right side view of the Invivo Plus workstation; Figure 8: Invivo Plus workstation right side view 1. Serial number /Rating label. 2. Portable appliance test (PAT test) label. 3. Mixed gas inlet. 4.

  • Page 17: Control System Layout

    Invivo Plus Control system layout The control system layout varies by model. Please familiarise yourself with the control system layout of the Invivo Plus workstation. 3.3.1 Control panel – Standard humidity control Figure 9 shows the control panel of Invivo Plus workstations equipped with standard humidity control;...

  • Page 18: Control Panel - Ultrasonic Humidity Control

    Invivo Plus 19. Socket on indicator. Illuminates to indicate that the internal socket is switched on. 20. Condenser fan indicator. Illuminates to indicate the condenser fan is running. 3.3.2 Control panel – Ultrasonic humidity control Ultrasonic humidity control is available as an optional accessory on the Invivo Plus.

  • Page 19: Temperature Controller / Ultrasonic Humidity Controller

    Invivo Plus 19. Interlock cycle status purge. Illuminates to indicate that the interlock purge cycle is in progress. 20. Interlock cycle status anaerobic. Illuminates to indicate that the interlock chamber is in an anaerobic condition. Note: For both control panels, the ANO , Com.

  • Page 20: Interlock Internal Door Control Panel

    Invivo Plus 3.3.4 Interlock internal door control panel Figure 12 shows the interlock internal door control panel; Figure 12: Interlock internal door control panel 1. Inner door LED indicator. Illuminates to indicate when the interlock inner door is available. 2. Inner door button. Press to unlock the interlock inner door (when available). UM-14 Version R3.0 Page 20 Invivo...

  • Page 21: Interlock Outer Door Control Panel

    Invivo Plus 3.3.5 Interlock outer door control panel Figure 13 shows the interlock outer door control panel; Figure 13: Interlock outer door control panel 1. Outer door open / close. Press individually to open the interlock outer door. Press and hold with outer door close (item 3) to close the interlock outer door.

  • Page 22: Power Entry Panel

    Invivo Plus 3.3.7 Power entry panel Figure 15 shows the power entry panel; Figure 15: Power entry panel 1. Gas mixer umbilical connection. 2. Earth Leakage Circuit Breaker (ELCB). 3. Nitrogen inlet 4. Primary earth stud. 5. Mains fuse holder. 6.

  • Page 23: Temperature And Humidity Control

    Invivo Plus Temperature and humidity control Temperature control The Invivo Plus workstation can control the workstation chamber temperature between ambient plus 5 C and 45 The temperature in the main chamber can be set using the temperature controller on the control panel.
  • Page 24 Invivo Plus The environmental gas composition of the workstation chamber is controlled by the ICO Advanced Gas mixing system. For more information, please see UM-011 ICO IC user manual for more details. UM-14 Version R3.0 Page 24 Invivo Plus User Manual...

  • Page 25: Using The Workstation Chamber

    Invivo Plus Using the workstation chamber Using the interlock The Invivo Plus workstation has a 28.8 litre interlock for transferring materials and samples into and out of the workstation chamber. Invivo Plus workstations can enable a purge facility in the interlock when placing items into the workstation.

  • Page 26: Removing Material From The Workstation Chamber Via The Interlock

    Invivo Plus • Place the required items on the interlock tray. The weight limit for the interlock floor tray is 4kg. • Close the interlock outer door. • Start the interlock purge cycle by pressing the interlock cycle start button (item 2 in Figure •...

  • Page 27: Vacuum Operation

    Invivo Plus Note: The workstation should not be used without the Ezee Sleeves attached. Figure 16: Ezee glove port and sleeve 6.2.2 Vacuum Operation To ensure that no external atmosphere contaminates the workstation, a single vacuum operation is required before Glove Port access. To minimise the time it is recommended to eliminate as much external atmosphere from the sleeve as possible prior to arm entry.
  • Page 28 Invivo Plus Note: Failure to grasp the Handle at this stage will make grasping it after the vacuum operation much more difficult. Figure 18: Arm inserted into sleeve (Shown with and without sleeve) 1. Glove port handle Figure 19: Foot pedals for right and left glove ports 1.

  • Page 29: Workstation Entry

    Invivo Plus The vacuum operation should be continued until the maximum amount of external atmosphere has been removed from the sleeve and the sleeve exerts pressure on the arm/hand. This take approx 15 to 20 seconds. Figure 21: Sleeve before vacuum operation Figure 20: Sleeve after vacuum operation The Sleeve should pull forcefully against both the inner surface of the Glove Port interior and the users arm and hand when adequate vacuum has been achieved.
  • Page 30 Invivo Plus Once the vacuum has been achieved, the Glove Port Handle can now be rotated in either direction to unlock the Glove Port Cap. As there is a strong vacuum within the Sleeve, removal of the Cap can require a reasonable amount of force.
  • Page 31 Invivo Plus Figure 25: Glove port cap storage location inside workstation Repeat the procedure for the other hand (if both hands are entering the workstation chamber). UM-14 Version R3.0 Page 31 Invivo Plus User Manual...

  • Page 32: Workstation Exit

    Invivo Plus Workstation Exit Remove Cap from storage brackets, and ensure the handle is oriented in a vertical position on the Cap. The Handle is designed with “indexing” detent features to help locate the Handle relative to the Cap. Drawing the handle into the Glove Port, replace the cap, using the location posts to orient the Cap correctly on the Glove Port.

  • Page 33: Single Plate Entry System (Spes)

    Invivo Plus Single Plate Entry System (SPES) A SPES is provided for quick and easy direct access to the workstation chamber, for loading materials. The SPES is also known as the mailbox. 6.4.1 SPES overview The SPES consists of an external hinged flap and an internal hinged flap. The external flap is held in place by a thumb screw when not in use.

  • Page 34: Using The Spes

    Invivo Plus Figure 28 shows the internal view of the SPES; Figure 28: SPES internal view 6.4.2 Using the SPES To use the SPES; • Undo the thumb screw on the external flap (item 1 in Figure 27). • Whilst supporting the SPES external flap, swing the thumb screw to the left. •...

  • Page 35: Internal Power Socket

    Invivo Plus Internal power socket An internal power socket is provided within the workstation chamber. The socket is located on the left hand side, underneath the rear shelf. The maximum permissible power rating of equipment connected to the internal socket is shown in Figure 30;...

  • Page 36: Optional Accessories

    Invivo Plus Optional accessories Your Invivo Plus workstation may be fitted with optional accessories to provide added functionality. The available optional accessories for Invivo Plus workstations are; • Cable gland. • Universal cable gland. • Gas sample port. • Ultrasonic humidity control. •...

  • Page 37: Gas Sample Port

    Invivo Plus Figure 32: Multi port 6.8.3 Gas sample port The gas sample port can be used to collect a gas sample from the workstation chamber. To use the gas sample port; • Remove the outer cap. • Push a needle connected to a syringe through the internal sponge of the gas sample port. •...
  • Page 38 Invivo Plus Figure 34: Internal part of vacuum port • When the vacuum is no longer required, remove the internal vacuum hose from the vacuum port by pressing the top of the metal part of the internal section of the vacuum port and pulling the vacuum hose.

  • Page 39: Online Video User Guides

    Invivo Plus Online video user guides Further information and demonstrations can be found at Ruskinn Technology Limited’s YouTube channel; http://www.youtube.com/ruskinntechnology Video demonstrations are available for; • Entering and exiting the workstation chamber via the Ezee Sleeves. • Setting oxygen and carbon dioxide values. •...

  • Page 40: Cleaning And Maintenance

    Invivo Plus Cleaning and maintenance Cleaning the workstation To ensure that your Invivo Plus workstation remains at optimum working conditions, it must be cleaned on a regular basis. A basic clean is required after each use. Deep cleaning is required at regular intervals, dependent upon the nature of the materials used in the workstation.

  • Page 41: Removing/ Reinstalling The Front Screen

    Invivo Plus Removing/ reinstalling the front screen Invivo Plus is fitted with a removable front screen, to facilitate easier cleaning and installation/ removal of equipment to and from the workstation chamber. To remove the front screen; • Remove all cells/ samples to an alternative storage facility. •...
  • Page 42 Invivo Plus • The left hand wall. • The right hand wall. • The interlock inner door. • The inside of the SPES. • The glove port covers. • For all workstation components, wipe with a paper towel soaked in an appropriate cleaning agent.

  • Page 43: Maintaining The Workstation - End User Maintenance

    Invivo Plus Maintaining the workstation – End user maintenance To ensure that your Invivo Plus workstation remains at optimum working conditions, it must be maintained on a regular basis. Many basic tasks can be performed by the end user. 7.4.1 Filling the pressure relief/ humidifier tank The pressure relief/ humidifier tank requires refilling if the water level is on or below the low level indication.

  • Page 44: Replacing An Ezee Sleeve

    Invivo Plus 7.4.2 Replacing an Ezee Sleeve To remove an Ezee Sleeve; • Ensure that the glove port covers are closed. • Remove the O-rings that hold the Ezee Sleeve to the glove port. Note that the O-rings will be tight.
  • Page 45 Invivo Plus To replace the detox sachets via the interlock; • Remove the new detox sachets from their packaging. • Open the interlock outer door (see section 6.1.2, for more details). • Place the new detox sachets in the interlock chamber. •...

  • Page 46: Installing/ Replacing A Catalyst Sachet

    Invivo Plus To reinstall the detox sachets with the front screen removed; • Remove the new detox sachets from their packaging. • Whilst lifting the front of the floor tray, slide the detox sachets into the detox sachet holders. • Lower the front of the floor tray back to its original position.
  • Page 47 Invivo Plus • Remove the old detox sachets. • Close the interlock outer door (see section 6.1.2, for more details). To install a catalyst sachet with the front screen removed; • Remove all cells/ samples to an alternative storage facility. •...

  • Page 48: Replacing The Mains Plug Fuse - Uk Users Only

    Invivo Plus 7.4.5 Replacing the mains plug fuse – UK users only To replace the mains plug fuse; • Switch off Invivo Plus and disconnect from the mains power supply. • Remove the plug from the mains socket. • Using a small flat bladed screw driver, remove the fuse cover from the mains plug. Figure 37 shows the fuse removal;...

  • Page 49: Replacing The Mains Fuses

    Invivo Plus 7.4.6 Replacing the mains fuses To replace the mains fuses; • Remove the plug from the mains socket. • Remove the mains fuse drawer using a small flat bladed screwdriver. Figure 38 shows the removal of the mains fuse holder; Figure 38: mains fuse holder removal •...

  • Page 50: Service Requirements

    Invivo Plus Service requirements To maintain the best performance from your Invivo Plus workstation, it must be serviced at regular intervals. Figure 39 lists the servicing requirements, intervals and persons capable of performing the service; Figure 39: Invivo2 Plus servicing requirements Action Frequency Clean workstation...

  • Page 51: Spare Parts And Cleaning Agents

    Invivo Plus 7.6.2 Spare parts and cleaning agents Figure 40 lists the spare parts and cleaning agents available for your Invivo Plus workstation. To order spare parts, please contact your local distributor for the latest pricing and availability. All items are sold individually except where stated.

  • Page 52: Accessories

    Invivo Plus 7.6.3 Accessories A range of accessories are available to enhance the functionality of your Invivo Plus workstation. Figure 41 is the list of accessories for the Invivo Plus workstation. Please contact your local distributor for the latest pricing and availability. Figure 41: Invivo2 Plus accessories Item Description...

  • Page 53: Common Problems And Solutions

    Invivo Plus Common problems and solutions 7.8.1 Workstation general problems Figure 42: Common problems and solutions Figure 42 gives a list of common problems and solutions. Please consult this list as a first reference in the event of a malfunction of your workstation. Figure 42: Common problems and solutions Problem Cause...

  • Page 54: Interlock Problems

    Invivo Plus 7.8.2 Interlock problems Figure 43 gives a list of common interlock problems and solutions; Figure 43: Interlock problems and solutions Problem Cause Solution The interlock inner The interlock outer Close the interlock outer door and run the door will not open door is open purge cycle The interlock has not...

  • Page 55: Warranty Information

    Invivo Plus Warranty information Ruskinn Technology Limited warrants for the applicable time period that the Invivo Plus will substantially perform in accordance with the user documentation. The terms of this Agreement do not affect or prejudice the statutory rights of a consumer acquiring the Ruskinn Technology Limited Invivo otherwise than in the normal course of a business.

  • Page 56: Disposal Information

    Invivo Plus Disposal information Invivo Plus contains hazardous components and must not be disposed of at a household waste site. Instead it should be taken to the appropriate collection point for the recycling of electrical and electronic equipment. Alternatively, please contact your local distributor for disposal instructions. Invivo Plus contains recyclable parts.

  • Page 57: Contact Details

    Ruskinn Technology Limited is a registered company in United Kingdom, company number 05692599 Ruskinn Technology Limited is VAT registered in United Kingdom, VAT number 870194126 Ruskinn Technology Limited is a wholly owned subsidiary of the Baker Company UM-14 Version R3.0...

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