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Related Manuals for Baker Bugbox Plus UM-017
Summary of Contents for Baker Bugbox Plus UM-017
- Page 1 Bugbox Plus UM-017 Bugbox Plus User Manual Affix Serial Number Sticker Here...
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Page 2: Product Summary
Bugbox Plus Product Summary Baker Ruskinn’s anaerobic workstations are designed specifically to help microbiologists cope with rising workloads and provide the best primary isolation rates. The world's smallest microaerophilic workstation packs all of the innovative features into a compact bench print. -
Page 3: Table Of Contents
Bugbox Plus Table of Contents Product Summary ........................... 2 Features and Benefits ..........................2 Overview ............................ 6 Safety Instructions ......................6 Regulatory compliance ....................7 Symbols ......................... 7 Installation and relocation .................... 8 Weight and dimensions ....................9 Gas and electrical supply requirement ..................10 Gas supply requirements .................... - Page 4 Bugbox Plus Humidity control ......................21 Standard humidity control overview .............. 21 Environmental gas composition control .................. 22 Environmental control overview ................22 Using the anaerobic indicator strips ................22 Using the workstation ......................23 Using the interlock ...................... 23 Interlock overview ..................
- Page 5 Bugbox Plus Replacing an Ezee Sleeve ................36 Replacing the detox and catalyst sachets ............37 Replacing the mains plug fuse – UK users only ..........38 Replacing the mains fuses ................39 Service requirements ....................40 Spare parts and accessories ..................40 Overview ......................
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Page 6: Overview
Bugbox Plus Overview Please read this manual carefully to familiarise yourself with the operation and maintenance of your Bugbox Plus workstation. Note: The workstation should be located in a well-ventilated area. Safety Instructions For your safety, the safety of others using the workstation and those around you; ... -
Page 7: Regulatory Compliance
Bugbox Plus Regulatory compliance This product complies with the essential EEA requirements for Electrical Safety and the Low Voltage Directive 2006/95/EC as well as Electromagnetic compatibility as set out in the EMC Directive 204/108/EC. Symbols Before using the Bugbox Plus, please ensure that you are familiar with the symbols on the Bugbox Plus. -
Page 8: Installation And Relocation
Bugbox Plus Symbol Meaning Warning, this equipment contains high voltage circuitry. Bugbox Plus contains hazardous components and must not be disposed of at a household waste site. Instead it should be taken to the appropriate collection point for the recycling of electrical and electronic equipment. -
Page 9: Weight And Dimensions
Bugbox Plus Weight and dimensions The Bugbox Plus workstation weighs approximately 55kg. Figure 2 lists the dimensions of the Bugbox Plus workstation; Figure 2: Bugbox Plus workstation dimensions External width 830 mm External height 650 mm External depth 660 mm Workstation chamber internal width 500 mm Workstation chamber internal height... -
Page 10: Gas And Electrical Supply Requirement
Bugbox Plus Gas and electrical supply requirement Gas supply requirements The Bugbox Plus workstation requires 2 gas supplies; Anaerobic mixed gas (up to 5.5% hydrogen, up to 10% carbon dioxide, remainder nitrogen) Oxygen free nitrogen. The maximum permissible concentration of hydrogen in the anaerobic mixed gas is 5.5%. The use of any other gas(ses) will invalidate the warranty and may cause serious injury. -
Page 11: Powering The Workstation
Bugbox Plus Powering the workstation To switch the workstation on, press the power switch (item 2 in Figure 13). To switch the workstation off, press the power switch (item 2 in Figure 13 ). Note: A glove port should be left open when the workstation is switched off, as the pressure inside the workstation will decrease as the workstation cools. -
Page 12: Workstation Overview
Bugbox Plus Workstation overview Workstation layout The Bugbox Plus workstation consists of 2 main areas; the workstation chamber and the interlock chamber. The workstation chamber is the main working area of the workstation. Access to the workstation chamber is provided by the glove ports and the interlock. The interlock consists of the interlock chamber, with an inner and an outer door. -
Page 13: Rear View
Bugbox Plus Rear view Figure 5 shows the rear view of the Bugbox Plus workstation; Figure 5: Bugbox Plus workstation rear view UM-17 R4.0 Page 13 of 47 Bugbox Plus User Manual... -
Page 14: Left Side View - Standard Humidifier
Bugbox Plus Left side view – Standard humidifier Figure 6 shows the left side view of the Bugbox Plus workstation with the standard humidifier; Figure 6: Bugbox Plus workstation left side view 1. Pressure relief tank fill level indicator. 2. Pressure relief tank. 3. -
Page 15: Left Side View - Ultrasonic Humidifier
Bugbox Plus Left side view – Ultrasonic humidifier Figure 7 shows the left side view of the Bugbox Plus workstation with the ultrasonic humidifier; Figure 7: Bugbox Plus workstation left side view with ultrasonic humidifier 1. Humidifier screw cap. 2. Humidifier tank. 3. -
Page 16: Right Side View
Bugbox Plus Right side view Figure 8 shows the right side view of the Bugbox Plus workstation; Figure 8: Bugbox Plus workstation right side view 1. Interlock outer door. 2. Serial number label. 3. PAT test label. 4. Power entry panel. UM-17 R4.0 Page 16 of 47 Bugbox Plus User Manual... -
Page 17: Control System Layout
Bugbox Plus Control system layout Please familiarise yourself with the control system layout of your Bugbox Plus workstation. Control panel Figure 9 shows the control panel of the Bugbox Plus workstation; Figure 9: Bugbox Plus workstation control panel 1. Power indicator. Illuminates to indicate the workstation is connected to the mains and is switched on. -
Page 18: Temperature Controller
Bugbox Plus Temperature controller Figure 10 shows the temperature controller; Figure 10: Temperature controller 1. Not user accessible (For service personnel only) 2. Not user accessible (For service personnel only) 3. Number scrolling button. Use to move across digits. Use with buttons 4 &... -
Page 19: Foot Switch Control Panel
Bugbox Plus Foot switch control panel Figure 12 shows the foot switch controller which is used to operate the spot light, vacuum control and interlock purge control; Figure 12: Foot switch controller 1. VAC for glove port vacuum control 2. LOCK for interlock purge control 3. -
Page 20: Interlock Inner Door Control Panel
Bugbox Plus Interlock inner door control panel Figure 14 shows the interlock inner door control panel; Figure 14: Interlock inner door control panel 1. Interlock inner door LED indicator. Illuminates when the interlock inner door is open. 2. Interlock inner door button. Press to open the interlock inner door (when available). UM-17 R4.0 Page 20 of 47 Bugbox Plus User Manual... -
Page 21: Temperature And Humidity Control
Bugbox Plus Temperature and humidity control Temperature control The Bugbox Plus workstation can control the workstation chamber temperature between ambient plus 5 C and 45 The temperature in the main chamber can be set using the temperature controller on the control panel. -
Page 22: Environmental Gas Composition Control
Bugbox Plus Environmental gas composition control The Bugbox Plus workstation provides an anaerobic (oxygen free) environment for the incubation of samples and cultures. Environmental control overview To provide an anaerobic environment, anoxic mixed gas is injected into the workstation chamber. The hydrogen in the anaerobic mixed gas reacts with any oxygen in the workstation chamber in the presence of a catalyst to form water. -
Page 23: Using The Workstation
Bugbox Plus Using the workstation Using the interlock The Bugbox workstation has a 6.5 litre interlock for transferring materials and samples into and out of the workstation chamber. Interlock overview The interlock consists of 3 main components; Interlock outer door ... -
Page 24: Transferring Material Into The Workstation Chamber Via The Interlock
Bugbox Plus To close the interlock inner door, slide the inner door forwards. Note: The interlock inner door should only be open when transferring materials from the interlock chamber to the workstation chamber and vice versa. The interlock inner door should not be opened if the interlock outer door is open. -
Page 25: Hand Access To The Main Chamber
Bugbox Plus Hand access to the main chamber Direct hand access to the workstation chamber is provided via the glove ports and the Ezee Sleeve system. The Ezee Sleeves provide direct bare hand (or gloved) access to the workstation chamber. The glove ports and Ezee Sleeves can be used in 3 ways;... -
Page 26: Ezee Sleeve Overview
Bugbox Plus Ezee Sleeve overview The Ezee Sleeves consists of a sleeve and a cuff. There are 3 sizes of Ezee Sleeve available; small, medium and large. See section 7.4.2 for ordering details. The sleeve attaches to the glove port via 2 O-rings. The cuff is then attached to the sleeve via an O- ring. -
Page 27: Exiting The Workstation Chamber Using The Ezee Sleeves
Bugbox Plus Whilst holding the glove port cover knob, push the glove port cover and your hand into the workstation chamber slowly. Place the glove port cover in the glove port cover holder. shows the glove port cover holder; Figure 18: Glove port cover holder Exiting the workstation chamber using the Ezee Sleeves To exit the workstation chamber using the Ezee Sleeves;... -
Page 28: Internal Lights
Bugbox Plus Internal lights 2 internal lighting systems are provided for the workstation chamber; Main chamber light. Spot light. To switch the main chamber light on, press the chamber light switch (item 2 in Figure 9). To switch the main chamber light off, press the chamber light switch (item 2 in Figure 9). To switch the spotlight on, press and hold the spot pedal on the foot switch control panel (item 3 in Figure 12). -
Page 29: Optional Extras
Bugbox Plus Optional extras Your Bugbox Plus workstation may be fitted with optional extra parts to provide added functionality. The available optional extra parts for Bugbox Plus workstations are; Single Plate Entry System (SPES). Internal socket. Cable gland. ... - Page 30 Bugbox Plus Figure 20 shows the internal view of the SPES; Figure 20: SPES internal view 6.5.1.2 Using the SPES To use the SPES; Undo the thumb screw on the external flap (item 1 in Figure 19). Whilst supporting the SPES external flap, swing the thumb screw to the left. ...
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Page 31: Internal Power Socket
Bugbox Plus Internal power socket An internal power socket can be provided within the workstation chamber. The socket is located on the left hand side, underneath the rear shelf. The maximum permissible power rating of equipment connected to the internal socket is shown in Figure 22. -
Page 32: Gas Sample Port
Bugbox Plus Gas sample port The gas sample port can be used to collect a gas sample from the workstation chamber. To use the gas sample port; Remove the outer cap. Push a needle connected to a syringe through the internal sponge of the gas sample port. ... -
Page 33: Cleaning And Maintenance
Bugbox Plus Cleaning and maintenance Cleaning the workstation To ensure that your Bugbox Plus workstation remains at optimum working conditions, it must be cleaned on a regular basis. A basic clean is required after each use. Deep cleaning is required at regular intervals, dependent upon the nature of the materials used in the workstation. -
Page 34: Cleaning Procedure - Deep Clean
Bugbox Plus Cleaning procedure – deep clean To deep clean the workstation; Preparing the workstation Remove all cells/ samples to an alternative storage facility. Switch the workstation off at the mains and remove the plug from the mains. ... -
Page 35: Maintaining The Workstation - End User Maintenance
Bugbox Plus Maintaining the workstation – End user maintenance To ensure that your Bugbox Plus workstation remains at optimum working conditions, it must be maintained on a regular basis. Many basic tasks can be performed by the end user. Filling the pressure relief/ humidifier tank The pressure relief/ humidifier tank requires refilling if the water level is on or below the low level indication. -
Page 36: Replacing An Ezee Sleeve
Bugbox Plus Replacing an Ezee Sleeve To remove an Ezee Sleeve; Ensure that the glove port covers are closed. Remove the O-rings that hold the Ezee Sleeve to the glove port. Note: The O-rings will be tight. Figure 28 shows the O-ring removal; Figure 28: O-ring removal ... -
Page 37: Replacing The Detox And Catalyst Sachets
Bugbox Plus Replacing the detox and catalyst sachets A detox and a catalyst sachet are supplied with the Bugbox Plus workstation. The detox sachet adsorbs volatile organic compounds, improving the air quality within the workstation chamber. The catalyst sachet contains the catalyst required for the hydrogen to react with any oxygen in the workstation, to produce an anoxic environment. -
Page 38: Replacing The Mains Plug Fuse - Uk Users Only
Bugbox Plus Whilst lifting the floor tray at the front, slide the sachets into the sachet holder. Lower the floor tray, ensuring that the floor tray leg has located in the floor tray locator (item 3 in Figure 29). ... -
Page 39: Replacing The Mains Fuses
Bugbox Plus Replacing the mains fuses To replace the mains fuses; Switch the Bugbox Plus off. Remove the plug from the mains socket. Remove the mains fuse drawer using a small flat bladed screwdriver. Figure 31 shows the removal of the mains fuse holder;... -
Page 40: Service Requirements
Bugbox Plus Service requirements To maintain the best performance from your Bugbox Plus workstation, it must be serviced at regular intervals. Figure 32 lists the servicing requirements, intervals and persons capable of performing the service; Figure 32: Bugbox Plus servicing requirements Action Frequency Clean workstation... -
Page 41: Spare Parts And Cleaning Agents
Bugbox Plus Spare parts and cleaning agents Figure 33 lists the spare parts and cleaning agents available for your Bugbox Plus workstation. To order spare parts, please contact your local distributor for the latest pricing and availability. All items are sold individually except where stated. -
Page 42: Workstation Malfunction
Bugbox Plus Workstation malfunction In the event of a workstation malfunction, please check section 7.6.1 for a list of common problems and solutions. If you cannot find a solution to your problem, please contact your local distributor, quoting the serial number of your workstation. Common problems and solutions Workstation general problems Figure 35 gives a list of common problems and solutions. -
Page 43: Interlock Problems
Bugbox Plus Problem Cause Solution The spot light does not The bulb has blown Contact your local distributor to arrange illuminate when replacement switched on The fuse has blown Plates are drying out little humidity Place a tray of water inside the workstation as a inside workstation water source. -
Page 44: Gas Consumption/ Environmental Control Problems
Bugbox Plus Gas consumption/ environmental control problems Bugbox Plus is fitted with a gas demand indicator (item 7 in Figure 9). The indicator illuminates when gas is injected into the workstation chamber. When the workstation is at rest, the gas demand indicator should illuminate once every 20 minutes. -
Page 45: Warranty Information
Bugbox Plus Warranty information Ruskinn Technology Limited warrants for the applicable time period that the Bugbox Plus will substantially perform in accordance with the user documentation. The terms of this Agreement do not affect or prejudice the statutory rights of a consumer acquiring the Ruskinn Technology Limited Bugbox Plus otherwise than in the normal course of a business. -
Page 46: Disposal Information
Bugbox Plus Disposal information Bugbox Plus contains hazardous components and must not be disposed of at a household waste site. Instead it should be taken to the appropriate collection point for the recycling of electrical and electronic equipment. Alternatively, please contact your local distributor for disposal instructions. Bugbox Plus contains recyclable parts. -
Page 47: Contact Details
Ruskinn Technology Limited is a registered company in United Kingdom, company number 05692599 Ruskinn Technology Limited is VAT registered in United Kingdom, VAT number 870194126 Ruskinn Technology Limited is a wholly owned subsidiary of the Baker Company UM-17 R4.0 Page 47 of 47...