Bugbox M is the perfect way to improve isolation rates and is an easy to justify alternative to anaerobic jars providing a more stable environment. Baker Ruskinn recognise that most general laboratories inoculate samples with cultures on the bench. That's why samples are transferred, at up to 10 at a time in the colour coded petri tubes - 15 seconds is all it takes to transfer and guarantee the best isolation rates for your Microaerophilic organisms.
Bugbox M Table of Contents Product Summary ........................... 2 Features and Benefits ..........................2 Overview ............................ 6 Safety Instructions ......................6 Regulatory compliance ....................7 Symbols ......................... 7 Installation and relocation .................... 8 Weight and dimensions ....................9 Gas and electrical supply requirement ..................10 Gas supply requirements ....................
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Bugbox M Environmental gas composition control .................. 19 Using the workstation ......................20 Using the interlock ...................... 20 Interlock overview ..................20 Opening the interlock outer door ..............20 Opening the interlock inner door ..............20 Transferring material into the workstation chamber via the interlock ..21 Removing material from the workstation chamber via the interlock ....
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Bugbox M Installing/ replacing a catalyst sachet ............. 36 Replacing the mains plug fuse – UK users only ..........37 Replacing the mains fuses ................38 Service requirements ....................39 Spare parts and accessories ..................39 Overview ......................39 Spare parts and cleaning agents ..............39 Accessories......................
Bugbox M Overview Please read this manual carefully to familiarise yourself with the operation and maintenance of your Bugbox M workstation. Note: The workstation should be located in a well-ventilated area. Safety Instructions For your safety, the safety of others using the workstation and those around you; ...
Bugbox M Regulatory compliance This product complies with the essential EEA requirements for Electrical Safety and the Low Voltage Directive 2006/95/EC as well as Electromagnetic compatibility as set out in the EMC Directive 204/108/EC. Symbols Before using the Bugbox M, please ensure that you are familiar with the symbols on the Bugbox M. Figure 1 explains the symbols found on the Bugbox M;...
Bugbox M Symbol Meaning Warning, this equipment contains high voltage circuitry. Bugbox M contains hazardous components and must not be disposed of at a household waste site. Instead it should be taken to the appropriate collection point for the recycling of electrical and electronic equipment.
Bugbox M Weight and dimensions The Bugbox M workstation weighs approximately 65kg. Figure 2 lists the dimensions of the Bugbox M workstation; Figure 2: Bugbox M workstation dimensions External width 800 mm External height 650 mm External depth 660 mm Workstation chamber internal width 500 mm Workstation chamber internal height...
Bugbox M Gas and electrical supply requirement Gas supply requirements The Bugbox M workstation requires: Oxygen free nitrogen. In addition to this, the Bugbox M workstation should be connected to ICO IC (supplied with the Bugbox M workstation). All other gas connections should be made to the ICO See UM-011 ICO IC user manual for more details.
Bugbox M Powering the workstation To switch the workstation on, press the power switch (item 2 in Figure 12). To switch the workstation off, press the power switch (item 2 in Figure 12). Note: A glove port should be left open when the workstation is switched off, as the pressure inside the workstation will decrease as the workstation cools.
Bugbox M Workstation overview Workstation layout The Bugbox M workstation consists of 2 main areas; the workstation chamber and the interlock chamber. The workstation chamber is the main working area of the workstation. Access to the workstation chamber is provided by the glove ports and the interlock. The interlock consists of the interlock chamber, with an inner and an outer door.
Bugbox M Rear view Figure 5 shows the rear view of the Bugbox M workstation; Figure 5: Bugbox M workstation rear view UM-20 R4.0 Page 13 of 47 Bugbox M User Manual...
Bugbox M Left side view – Standard humidifier Figure 6 shows the left side view of the Bugbox M workstation with the standard humidifier; Figure 6: Bugbox M workstation left side view 1. Pressure relief tank cover (pressure relief tank bung). 2.
Bugbox M Right side view Figure 7 shows the right side view of the Bugbox M workstation; Figure 7: Bugbox M right side view 1. Interlock outer door knob. 2. Interlock outer door. 3. Power entry panel. 4. Serial number label. 5.
Bugbox M Control system layout Please familiarise yourself with the control system layout of your Bugbox M workstation. Control panel Figure 8 shows the control panel of the Bugbox M workstation; Figure 8: Bugbox M workstation control panel 1. Power indicator. Illuminates to indicate the workstation is connected to the mains and is switched on.
Bugbox M Temperature controller Figure 9 shows the temperature controller; Figure 9: Temperature controller 1. Not user accessible (For service personnel only) 2. Not user accessible (For service personnel only) 3. Number scrolling button. Use to move across digits. Use with buttons 4 &...
Bugbox M Foot switch control panel Figure 11 shows the foot switch controller which is used to operate the spot light, vacuum control and interlock purge control. Figure 11: Foot switch control panel 1. VAC for glove port vacuum control 2.
Bugbox M Temperature and humidity control Temperature control The Bugbox M workstation can control the workstation chamber temperature between ambient plus C and 45 The temperature in the main chamber can be set using the temperature controller on the control panel.
Bugbox M Using the workstation Using the interlock The Bugbox M workstation has a specially designed interlock for transferring Petri dishes into and out of the workstation chamber. Interlock overview The interlock consists of 2 main components; Interlock outer door ...
Bugbox M Transferring material into the workstation chamber via the interlock To transfer material into the workstation chamber via the interlock; Place the Petri dishes in a small Petri dish holder. Open the interlock outer door (see section 6.1.2 for more details). ...
Bugbox M Hand access to the main chamber Direct hand access to the workstation chamber is provided via the glove ports and the Ezee Sleeve system. The Ezee Sleeves provide direct bare hand (or gloved) access to the workstation chamber. The glove ports and Ezee Sleeves can be used in 3 ways;...
Bugbox M Entering the workstation chamber using the Ezee Sleeves To enter the workstation via the Ezee Sleeves; Set the glove port selector switch (Figure 10) to the correct position for the desired hand entry Figure 13. Hold the desired Ezee Sleeve cuff with the opposite hand. ...
Bugbox M Figure 15: Glove port cover holder Exiting the workstation chamber using the Ezee Sleeves To exit the workstation chamber using the Ezee Sleeves; Remove the glove port cover from the glove port cover holder (Figure 15). Holding the glove port cover by the glove port cover knob, slowly pull the glove port cover into the glove port with the glove port bar horizontal.
Bugbox M Single Plate Entry System (SPES) A SPES is provided for quick and easy direct access to the workstation chamber, for loading materials. The SPES is also known as the mailbox. SPES overview The SPES consists of an external hinged flap and an internal hinged flap. The external flap is held in place by a thumb screw when not in use.
Bugbox M Using the SPES To use the SPES; Undo the thumb screw on the external flap (item 1 in Figure 16). Whilst supporting the SPES external flap, swing the thumb screw to the left. Lower the SPES external flap. Figure 18 shows the SPES when opened;...
Bugbox M Internal lights 2 internal lighting systems are provided for the workstation chamber; Main chamber light. Spot light. To switch the main chamber light on, press the chamber light switch (item 2 in Figure 8). To switch the main chamber light off, press the chamber light switch (item 2 in Figure 8). To switch the spotlight on, press and hold the spot pedal on the foot switch control panel (item 3 in Figure 11).
Bugbox M Cable gland The cable gland is used to allow cables to enter the workstation chamber without affecting the internal environment of the workstation chamber. The cable gland is suitable for cables of diameter 3.5mm to 7mm. The cable gland port is located on the left hand side. Figure 20 shows the cable gland;...
Bugbox M Gas sample port The gas sample port can be used to collect a gas sample from the workstation chamber. To use the gas sample port; Remove the outer cap. Push a needle connected to a syringe through the internal sponge of the gas sample port. ...
Bugbox M Online video user guides Further information and demonstrations can be found at Ruskinn Technology Limited’s YouTube channel; http://www.youtube.com/ruskinntechnology UM-20 R4.0 Page 30 of 47 Bugbox M User Manual...
Bugbox M Cleaning and maintenance Cleaning the workstation To ensure that your Bugbox M workstation remains at optimum working conditions, it must be cleaned on a regular basis. A basic clean is required after each use. Deep cleaning is required at regular intervals, dependent upon the nature of the materials used in the workstation.
Bugbox M Cleaning procedure – deep clean To deep clean the workstation; Preparing the workstation Remove all cells/ samples to an alternative storage facility. Switch the workstation off at the mains and remove the plug from the mains. ...
Bugbox M Maintaining the workstation –user maintenance To ensure that your Bugbox M workstation remains at optimum working conditions, it must be maintained on a regular basis. Many basic tasks can be performed by the end user. Filling the pressure relief/ humidifier tank The pressure relief/ humidifier tank requires refilling if the water level is on or below the low level indication.
Bugbox M Replacing an Ezee Sleeve To remove an Ezee Sleeve; Ensure that the glove port covers are closed. Remove the O-rings that hold the Ezee Sleeve to the glove port. Note that the O-rings will be tight. Figure 25 shows the O-ring removal; Figure 25: O-ring removal ...
Bugbox M Replacing detox sachets Detox sachets are supplied with the Bugbox M workstation. The detox sachets adsorb volatile organic compounds, improving the air quality within the workstation chamber. Detox sachets need to be replaced annually. See section 7.4.2 for ordering details. To replace the detox sachets;...
Bugbox M Lower the floor tray, ensuring that the floor tray leg has located in the floor tray locator (item.3 in Figure 26). Close the glove ports using the glove port covers. Reinstall the Ezee Sleeves (see section 7.2.2 for more details). ...
Bugbox M Replacing the mains plug fuse – UK users only To replace the mains plug fuse; Switch off Bugbox M and disconnect from the mains power supply. Remove the plug from the mains socket. Using a small flat bladed screw driver, remove the fuse cover from the mains plug. Figure 27 shows the fuse removal;...
Bugbox M Replacing the mains fuses To replace the mains fuses; Switch off the Bugbox M. Remove the plug from the mains socket. Remove the mains fuse drawer using a small flat bladed screwdriver. Figure 28 shows the removal of the mains fuse holder;...
Bugbox M Service requirements To maintain the best performance from the Bugbox M workstation, it must be serviced at regular intervals. Figure 29 lists the servicing requirements, intervals and persons capable of performing the service; Figure 29: Bugbox M servicing requirements Action Frequency Clean workstation...
Bugbox M Figure 30: Bugbox M end user spare parts and cleaning agents list Part Where used Small Ezee Sleeve (Pair) Ezee Sleeve Medium Ezee Sleeve (Pair) Ezee Sleeve Large Ezee Sleeve (Pair) Ezee Sleeve Glove port seal plate assembly Glove ports Sleeve to port O-ring Ezee Sleeve...
Bugbox M Workstation malfunction In the event of a workstation malfunction, please check section 7.6.1 for a list of common problems and solutions. If you cannot find a solution to your problem, please contact your local distributor, quoting the serial number of your workstation. If the problem is related to the ICO IC, please download the event log to a USB device (see UM-011 ICO IC user manual for more details) as it will...
Bugbox M Common problems and solutions Workstation general problems Figure 32 gives a list of common problems and solutions. Please consult this list as a first reference in the event of a malfunction of your workstation. Figure 32: Common problems and solutions Problem Cause Solution...
Bugbox M Interlock problems Figure 33 gives a list of common interlock problems and solutions; Figure 33: Interlock problems and solutions Problem Cause Solution The interlock inner interlock inner Check for obstructions to closing the interlock door will not close door is blocked inner door and remove them interlock inner...
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Bugbox M Problem Potential Causes Solution The interlock inner and Close either the interlock inner door or the The gas demand indicator outer doors are open at interlock outer door illuminates more than the same time Close the SPES. See section 0 for more details once every 20 minutes The SPES has been left whilst at rest/ high gas...
Bugbox M Warranty information Ruskinn Technology Limited warrants for the applicable time period that the Bugbox M will substantially perform in accordance with the user documentation. The terms of this Agreement do not affect or prejudice the statutory rights of a consumer acquiring the Ruskinn Technology Limited Bugbox M otherwise than in the normal course of a business.
Bugbox M Disposal information Bugbox M contains hazardous components and must not be disposed of at a household waste site. Instead it should be taken to the appropriate collection point for the recycling of electrical and electronic equipment. Alternatively please contact your local distributor for disposal instructions. Bugbox M contains recyclable parts.
Ruskinn Technology Limited is a registered company in United Kingdom, company number 05692599 Ruskinn Technology Limited is VAT registered in United Kingdom, VAT number 870194126 Ruskinn Technology Limited is a wholly owned subsidiary of the Baker Company UM-20 R4.0 Page 47 of 47...
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