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Operation Quick Reference Guide
Sampler Pre-run Checklist
Use this checklist to make sure the sampler is ready before you begin a run.
Sample vials are half full.
Cap is centered, no wrinkles, septum is flat.
Sample vial positions match the run parameters.
Tray quadrants snapped in place (with tray).
4.5 mL of fresh solvent in each solvent bottle.
Waste bottles are empty.
Two waste bottles (with tray).
There is enough solvent and waste capacity for your sample vials.
Syringe is new or clean.
Syringe design and size are correct.
Plunger is secure in plunger carrier loop.
Needle is aligned with septum retainer nut.
Syringe is rinsed with solvent.
GC inlet liner is clean and deactivated.
GC inlet liner is correct type for injection technique.
GC inlet septum type is correct and the septum is less than 200 injections old.
Correct septum nut is installed in GC inlet.
Correct nanoliter enable setting for required injection volume.
(GC or integrator control only)
Correct cool on-column inlet insert installed for your column/syringe.
Running a sample
To operate your 7683 Automatic Liquid Sampler:
1.
Install a clean syringe. See Installing A Syringe on page 16.
2.
Fill the solvent bottles and load them into the turret. See Preparing The Solvent And
Waste Bottles on page 13.
3.
Load the waste bottles into the turret. See Preparing The Solvent And Waste Bottles on
page 13.
4.
Load the sample vials into the turret or into the tray. See Preparing Sample Vials on
page 9.
5.
Load (or program) the GC sequence. See your 6890 GC or Agilent ChemStation
documentation.
6.
Run the GC sequence.
When the GC becomes ready, the 7683 Automatic Liquid Sampler begins the injections.
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Table of Contents
Related Manuals for Agilent Technologies 7683
Summary of Contents for Agilent Technologies 7683
- Page 1 Load the sample vials into the turret or into the tray. See Preparing Sample Vials on page 9. Load (or program) the GC sequence. See your 6890 GC or Agilent ChemStation documentation. Run the GC sequence. When the GC becomes ready, the 7683 Automatic Liquid Sampler begins the injections.
- Page 3 Operation Guide Agilent 7683 Automatic Liquid Sampler...
- Page 4 68 dB(A) during designed for use indoors. short burst injection pulses. Whenever the safety protection Part No. G2612-90117 CAUTION of the 7683 Automatic Liquid Schalldruckpegel Replaces Part No. G2612-90110 Sampler has been A caution calls attention to a compromised, disconnect the condition or possible situation Schalldruckpegel LP <...
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Page 5: Table Of Contents
Contents About your 7683 Automatic Liquid Sampler ........1 Capabilities ....................2 Injection Volume ................3 Pre-run Checklist ..................4 Running A Sample .................. 5 Interrupting A Run Or Sequence ............6 Sampler Response To Interruptions ..........6 Restarting An Interrupted Sequence ..........7 Running A Priority Sample .............. -
Page 7: About Your 7683 Automatic Liquid Sampler
Operation Guide About your 7683 Automatic Liquid Sampler About your 7683 Automatic Liquid Sampler The 7683 Automatic Liquid Sampler system, shown in Figure 1, can include: • One G2612A ALS interface board (6890 only) • One or 2 G2613A injector module(s) •... -
Page 8: Capabilities
Operation Guide Capabilities Capabilities Table 1 summarizes the capabilities of the 7683 Automatic Liquid Sampler. Table 1 Automatic Liquid Sampler Capabilities Parameter Range 5, 10, 25, 50, or 100 µL Syringe size Number of sample vials up to 8 without tray or 100 with tray... -
Page 9: Injection Volume
GC’s nanoliter enable setting is on or off. See Table 2. Table 2 Injection volumes for 6890 GC or integrator control 6890 Sample volume 7683 Injection volume (µL) setting (syringe stops) 5 µL 10 µL 25 µL 50 µL... -
Page 10: Pre-Run Checklist
Operation Guide Pre-run Checklist Pre-run Checklist Use this checklist to make sure the sampler is ready before you begin. Sample vials are half full. Cap is centered, no wrinkles, septum is flat. Sample vial positions match the run parameters. Tray quadrants snapped in place (with tray). 4.5 mL of fresh solvent in each solvent bottle. -
Page 11: Running A Sample
Load the sample vials into the turret or into the tray. See Preparing sample vials. Load (or program) the GC sequence. See your 6850/6890 GC or Agilent ChemStation documentation. Run the GC sequence. When the GC becomes ready, the 7683 Automatic Liquid Sampler begins the injections. -
Page 12: Interrupting A Run Or Sequence
6850/6890 or Agilent ChemStation If the interruption was caused by a problem that the sampler recognizes, a message will appear on the GC or Agilent ChemStation. For more information, refer to your Agilent 7683 Installation Guide and GC or Agilent ChemStation documentation. •... -
Page 13: Restarting An Interrupted Sequence
Operation Guide Interrupting A Run Or Sequence Restarting An Interrupted Sequence To restart an aborted sequence from the point of interruption: Resolve the problem that caused the interruption. Identify the last sample vial that was run successfully. (Check the vial number of the last successful chromatogram.) Assign the first sample vial run to be the vial number after the last successful one. -
Page 14: Running A Priority Sample
Running A Priority Sample Running A Priority Sample If the Use priority parameter is on in your 6890 GC sequence, 7683 Automatic Liquid Sampler tray gripper checks for a vial in position 100 before loading each new vial into the injector turret. If it finds a vial there, it will load that sample vial into the turret and run it according to the method you assigned for priority samples. -
Page 15: Preparing Sample Vials
Operation Guide Preparing Sample Vials Preparing Sample Vials Selecting Sample Vials The 7683 Automatic Liquid Sampler injector and the tray use clear or amber glass ® ™ sample vials with crimp caps, or Target screw-cap vials. Use amber glass vials for light-sensitive samples. Refer to your Agilent catalog for consumables and supplies for acceptable vial types. -
Page 16: Labeling Sample Vials
Preparing Sample Vials Labeling Sample Vials Vials are available with a write-on spot for easy marking. If you choose to make and apply your own labels, Agilent Technologies, Inc. recommends the positioning and maximum label thickness shown in Figure 3. No label 5.6 ±... -
Page 17: Filling Sample Vials
Operation Guide Preparing Sample Vials • Another is high-quality, low-extractable silicone rubber septa, coated with Teflon on one or both sides. This is more resistant to solvents after puncture and to coring by the needle. Refer to your Agilent catalog for consumables and supplies for more information. Figure 4 shows the diameter for vial cap apertures. -
Page 18: If You Use The Optional 100-Vial Tray
Operation Guide Preparing Sample Vials Solvent A Sample 1 Solvent A Sample 1 Solvent B Waste A Waste Solvent B Solvent B2 Sample 8 (or Sample 2*) Waste B or (Sample 3*) Standard turret 8 position turret *Requires optional insert (part no. 07673-40150) to hold sample vial. Figure 5 Vial placement in the turret If You Use The Optional 100-vial Tray... -
Page 19: Preparing The Solvent And Waste Bottles
Operation Guide Preparing The Solvent And Waste Bottles Preparing The Solvent And Waste Bottles Solvent bottles hold solvent for rinsing the syringe between injections. The injector discards the solvent washes and sample washes into waste bottles. The number of samples that can be analyzed may be limited by the wash or waste bottle capacity shown in Figure 7 and Figure 8. -
Page 20: Using Two Injectors
Operation Guide Using Two Injectors Using Two Injectors With two injectors mounted on the GC, you can use the front injector or the back injector or both injectors simultaneously. Two-injector configurations have the following characteristics: • The system sends one injector ready signal to the GC when both injectors are ready and one start signal at the beginning of the injection stroke. -
Page 21: Syringes
Operation Guide Syringes Syringes Inspecting A Syringe Before installing a syringe: Roll the syringe on the edge of a clean flat surface. If the tip of the needle moves in a circle, straighten the shaft by bending it slightly near where it connects to the syringe barrel and check it again. -
Page 22: Installing A Syringe
Operation Guide Syringes Installing A Syringe To install a syringe: Disconnect the injector cable from the GC and lay the injector on its back on a flat surface. Open the injector door. Slide the syringe carriage up (or down) until the syringe is accessible. See Figure 10. - Page 23 Operation Guide Syringes Move the plunger carrier loop down and tighten the plunger screw. Move the plunger carrier loop up and down. If the syringe plunger does not move along with the carrier, repeat the previous steps. Be sure the plunger screw is tight.
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Page 24: Removing A Syringe
Operation Guide Syringes Caution Do not operate the injector without a syringe in place because the syringe latch may interfere with the motor if it is allowed to swing freely. 10. Pull down the syringe carriage until the needle tip is near the top of the inlet septum nut. -
Page 25: Cool On-Column Injection
Cool On-column Injection Cool On-column Injection For an 6890 GC with a cool on-column inlet, the 7683 Automatic Liquid Sampler can perform injections directly onto 250-µm, 320-µm, and 530-µm columns. For a list of consumables needed to perform these injections, see Volume 2 of your GC Operating Manual. -
Page 26: Controlling Sample Vial Temperatures
Operation Guide Controlling Sample Vial Temperatures Controlling Sample Vial Temperatures This section explains how to connect tubing to the tray quadrants, how to determine the water bath temperature needed to obtain an approximate sample vial temperature, and the pressure limitations of the quadrants. This section does not explain how to set up a water bath and pump. - Page 27 Operation Guide Controlling Sample Vial Temperatures TEE manifold Outlet Connect ¼-inch tubing to underside drain fitting Tray front Tray back Inlet Figure 12 Tray quadrant plumbing Determine the water bath temperature The temperature of the sample vials depends on several factors, including the ambient temperature of the air around the tray and the temperature of the water bath.
- Page 28 Operation Guide Controlling Sample Vial Temperatures 40° C 30° C 25° C 20° C 15° C 10° C Ambient temperature Desired vial temperature (°C) Temperature of water bath (°C) Figure 13 Sample vial temperature versus water bath temperature for values of ambient air temperature...
- Page 29 Operation Guide Controlling Sample Vial Temperatures Tray quadrant temperatures and pressures Do not allow the tray inlet pressure and inlet temperature to exceed the limits shown in Figure 14. If you operate the bath outside this range, the quadrants could be damaged. Tray inlet pressure...
- Page 30 Operation Guide Controlling Sample Vial Temperatures Water bath and pump specifications The water bath and pump system used to control the sample vial temperatures must meet these specifications. • The components must meet national standards for safety requirements, be suitable for unattended operation, be suitable for continuous operation, and be controllable for high-temperature protection.