Pipette Techniques - Thermo Scientific Orion 2111LL User Manual

Pipette Techniques

Forward Technique
Thermo Scientific Orion 2111LL Sodium Monitor User Guide
ROSS and the COIL tradedress are trademarks of Thermo Fisher Scientific Inc. US Patent 6,793,787
Special care shall be taken to avoid liquid being aspirated into the interior
General working of the pipette. Push and release the push button slowly at all times,
particularly when working with high viscosity liquids. Never allow the push
button to snap back. Make sure that the tip is firmly attached to the tip
cone. Check for foreign particles in the tip.
• Do not touch pipette tip with bare fingers. Risk of sodium
contamination is possible. Use powder free gloves for protection.
Before you begin your actual pipetting work, fill and empty the tip 3-5
times with the solution that you will be pipetting. Hold the pipette in an
upright position while aspirating liquid. The grippy should rest on your
index finger. Make sure that the tips, pipette and solution are at the same
temperature.
Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button to the first stop.
2. Dip the tip under the surface of the liquid in the reservoir to a depth of
about 1 cm and slowly release the push button. Withdraw the tip from
the liquid touching it against the edge of the reservoir to remove excess
liquid.
3. Deliver the liquid by gently depressing the push button to the first stop.
After a delay of about one second, continue to depress the push button
all the way to the second stop. This action will empty the tip.
4. Release the push button to the ready position. If necessary, change the
tip and continue pipetting.
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Appendix
A-12