Related Manuals for Agilent Technologies PlateLoc
Summary of Contents for Agilent Technologies PlateLoc
- Page 1 PlateLoc Thermal Microplate Sealer Quick Reference Guide ...
- Page 2 Further, to the max- A WARNING notice denotes a prior agreement and written consent from imum extent permitted by applicable Agilent Technologies, Inc. as governed by hazard. It calls attention to an law, Agilent disclaims all warranties, United States and international copyright...
- Page 3 PlateLoc Thermal Microplate Sealer Reference for Optimal Thermal Microplate Sealing PlateLoc Thermal Microplate Sealer Reference for Optimal Thermal Microplate Sealing This reference contains the following topics: • “Optimizing the sealing quality” on page 2 • “Optimizing seal parameters” on page 5 •...
- Page 4 Sealing the wells protects the contents from evaporation, condensation, oxidation, and cross- contamination during transport or storage. The PlateLoc Sealer can be used as a standalone device or in a lab automation system. The device accepts microplates made from a variety of materials.
- Page 5 For example, place a flexible 96- well polypropylene microplate on top of a 96- well polystyrene microplate to provide positional support. Agilent Technologies also offers an adapter tray for PCR microplate support. Reference for Optimal Thermal Microplate Sealing...
- Page 6 Insert thickness Choose the right insert to support the microplate and ensure uniform sealing. A set of four inserts are presently shipping with every PlateLoc Sealer, and a special insert is available for Labcyte microplates. Reference for Optimal Thermal Microplate Sealing...
- Page 7 Note: Pierceable seals might permanently adhere to the microplate. Do not wait too long or the seals will be difficult to remove. Remove the microplate from the PlateLoc Sealer. Carefully peel off the seal material by lifting one of the corners of the seal as shown in Figure 6.
- Page 8 PlateLoc Thermal Microplate Sealer Optimizing seal parameters Figure 6 Removing the seal. Inspect the underside of the removed seal material. If there are unbroken impressions of each well on the underside of the seal material, the microplate was properly sealed.
- Page 9 PlateLoc Thermal Microplate Sealer Optimizing seal parameters If the well impressions on the seal are significantly thicker than the chimney wall (as shown in Figure 9), decrease the sealing temperature or duration. Thick impressions may indicate excessive melting of the seal, causing the seal to be difficult to remove and the resealing capacity reduced.
- Page 10 PlateLoc Thermal Microplate Sealer Requesting application support Requesting application support To help expedite your support request, you can complete and submit the Request for PlateLoc Sealer Application Support. For other questions, you can contact Automation Solutions Technical Support at one of the following:...
- Page 11 Is the PlateLoc Sealer compatible with other third- party sealing materials? Agilent Technologies does not verify, validate, test, or support the use of third- party sealing material on the PlateLoc Sealer. There is also some risk that non- Agilent Technologies sealing material may melt onto internal (non- user- serviceable) components, necessitating service.
- Page 12 PlateLoc Thermal Microplate Sealer User Guide. Maximum sealing temperature What is the highest temperature and longest duration I can set on my PlateLoc Sealer? The maximum temperature is 235 °C. The duration can be adjusted from 0.5 to 12 seconds.
- Page 13 Gas-purging option The samples we have are in DMSO and we plan to use the PlateLoc Sealer to seal our microplates with Agilent Technologies Peelable Aluminium Seal PN 06643.001 prior to putting our microplates into storage at –20 °C. How might...
- Page 14 Technologies recommends that you verify that the PlateLoc Sealer does not adversely affect your samples. Continuous (24/7) operation We intend to share our PlateLoc Sealer in a core lab. Is it OK to operate it 24/7? The PlateLoc Sealer is robust and designed for challenging, high- throughput, continuous- duty, production applications.
- Page 15 PlateLoc Thermal Microplate Sealer Frequently asked questions Evaporation verification and validation Do you have an established protocol we can use to test the PlateLoc seal for permeability and evaporation? A simple way to test for evaporation uses an experimental microplate and a control such as a 100- g weight.
- Page 16 Tall labware, such as deep- well microplates as tall as 2.5 in (63.5 mm), can be accommodated. High- volume storage and collection microplates exceeding 2.5 in are not compatible with the PlateLoc Sealer (for example, Porvair Sciences 48- well microplates of 7 and 10 mL sample volume, etc.).
- Page 17 Do you sell microplate seals that are certified to be sterile as well as RNase- / DNase- free? No, not at this time. The majority of PlateLoc Sealer customers have been able to use the existing materials. Agilent Technologies can develop seal materials with these features, provided the customer is willing to invest the time and substantial cost required to do so.
- Page 18 Application Support We have additional questions and need help with our applications. How can we request help? To help expedite your support request, you can complete and submit the Request for PlateLoc Sealer Application Support. Reference for Optimal Thermal Microplate Sealing...
- Page 20 Quick Reference Guide G5402-90003...