Agilent Technologies Small RNA Kit Quick Start Manual

The complete Small RNA Kit Guide can be found in the online help of the 2100 Expert software.
Agilent Small RNA Kit (reorder-no 5067-1548)
Agilent Small RNA Chips
25 Small RNA Chips
3 Electrode Cleaners
Syringe Kit
1 Syringe
Tubes for Gel-Dye Mix
30 Safe-Lock Eppendorf Tubes PCR clean (DNase/RNase free) for gel-dye mix
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this product conveys to the buyer the non-transferable right to use the purchased amount of the product and
components of the product only as described in accompanying product literature. The sale of this product is expressly
conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service,
information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes;
(4) to resell, sell or otherwise transfer this product or its components to any third party, or use for any use other than
use in the subfields of research and development, quality control, forensics, environmental analysis, biodefense or food
safety testing. For information on purchasing a license to this product for purposes other than described above contact
Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene,
OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354"
Assay Principles
Agilent RNA kits contain chips and reagents designed for analysis of RNA fragments. Each RNA chip
contains an interconnected set of microchannels that is used for separation of nucleic acid fragments based
on their size as they are driven through it electrophoretically. Agilent RNA kits are designed for use with the
Agilent 2100 Bioanalyzer instrument only.
Applications and Kits
Agilent Small RNA kits are designed for the analysis of total RNA (eukaryotic and prokaryotic) and samples
enriched for small nucleic acids.
Agilent RNA kits: RNA 6000 Nano Kit (reorder-no 5067-1511), RNA 6000 Pico Kit (reorder-no 5067-1513),
and Small RNA Kit (reorder-no 5067-1548).
Storage Conditions
• Freeze unopened Small RNA ladder at -20 °C. Prepared ladder aliquots need to be stored at -70 °C. Keep
all other reagents and reagent mixes refrigerated at 4 °C when not in use to avoid poor results caused by
reagent decomposition.
• Protect marker solution (contains a fluorescent dye), dye and dye mixtures from light. Remove light
covers only when pipetting. Dye decomposes when exposed to light.
Agilent Small RNA Kit
Quick Start Guide
Agilent Small RNA Reagents (reorder-no 5067-1549) & Supplies
(blue) Small RNA Dye Concentrate
(green) Small RNA Marker (4 vials)
(white) Small RNA Conditioning Solution
(red) Small RNA Gel Matrix (2 vials)
(yellow) Small RNA Ladder (reorder-no 5067-1550)
2 Spin Filters (reorder-no 5185-5990) + 30 tubes for gel-dye mix
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