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ORBECO MC500 Instruction Manual

Multi-parameter colorimeter
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MC500
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Multi-Parameter
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Instruction Manual

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Summary of Contents for ORBECO MC500

  • Page 1 MC500 Multi-Parameter Colorimeter Instruction Manual...

  • Page 3: Safety Precautions

    The accuracy of the instrument is only valid if the instrument is used in an environment with controlled electromagnetic disturbances according to DIN 61326. Wireless devices, e.g. wireless phones, should not be used near the instrument. Revision_2d 06/2010 MC500 Colorimeter 06-2010...

  • Page 4: Table Of Contents

    Chlorine HR (Kl) ...............................60 Chlorine UHR......................See Sodium Hypochlorite COD, low range (LR) ...............................62 COD, middle range (MR) ............................64 COD, high range (HR) ............................66 Color ....................................68 Copper with tablet ..............................70 differentiated determination (free, combined, total) ................71 free Copper ................................72 MC500 Colorimeter 06-2010...
  • Page 5 PHMB (Biguanide) ............................... 142 Phosphate ................................144 Phosphate, ortho LR with tablet ....................... 146 Phosphate, ortho HR with tablet ......................148 Phosphate, ortho (powder packs) ......................150 Phosphate, ortho (tube test) ........................152 Phosphate 1, ortho C ............................ 154 MC500 Colorimeter 06-2010...
  • Page 6 Saving data – Important Notes ....................206 2.1.3 Replacement of batteries ......................206 2.1.4 Instrument (explosion drawing) ....................207 Overview of function keys ......................208 2.2.1 Overview ............................208 2.2.2 Displaying time and date ......................209 2.2.3 User-countdown ........................... 209 MC500 Colorimeter 06-2010...
  • Page 7 Instrument basic settings 2 ....................... 252 2.4.10 Instrument special functions / Service .................. 252 Data transfer ........................... 253 2.5.1 Print of data ............................. 253 2.5.2 Data transfer to a personal computer (PC) ................253 2.5.3 Internet-Updates ........................... 253 MC500 Colorimeter 06-2010...
  • Page 8 Unpacking ............................256 Delivery content ..........................256 blank because of technical requirements Technical data..........................257 Abbreviations ..........................258 Troubleshooting ..........................259 3.6.1 Operating messages in the display / error display ............259 3.6.2 General problems ......................... 262 MC500 Colorimeter 06-2010...

  • Page 9: Part 1 Methods

    Part 1 Methods MC500 Colorimeter 06-2010...

  • Page 10: Table Of Methods

    * = free, combined, total; PP = powder pack; T = tablet; L = liquid; TT = tube test; LR = low range; MR = middle range; HR = high range; Vacu-vial is a registered trade mark of CHEMetrics Inc. ® MC500 Colorimeter 06-2010...
  • Page 11 * = free, combined, total; PP = powder pack; T = tablet; L = liquid; TT = tube test; LR = low range; MR = middle range; HR = high range; Vacu-vial is a registered trade mark of CHEMetrics Inc. ® MC500 Colorimeter 06-2010...
  • Page 12 * = free, combined, total; PP = powder pack; T = tablet; L = liquid; TT = tube test; LR = low range; MR = middle range; HR = high range; Vacu-vial is a registered trade mark of CHEMetrics Inc. ® MC500 Colorimeter 06-2010...
  • Page 13 2) The Symbol "Ø" signifies the OUTSIDE Diameter of the Sample Vial 3) Please note the appropriate reagent platform (tablet, powder, vial, etc.) for each test. 4) Be sure to observe the proper reagent reaction time indicated for each procedure for accurate results. MC500 Colorimeter 06-2010...

  • Page 14: Acid Demand To Ph 4.3

    7. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted prepare Test 8. Press TEST key. press TEST The result is shown in the display as Acid demand to pH 4.3 in mmol/l. MC500 Colorimeter 06-2010...
  • Page 15 1.1 Methods Notes: 1. The terms total Alkalinity, Alkalinity-m, m-Value and Acid demand to pH 4.3 are identical. 2. For accurate results, be sure to test on exactly 10 ml of water sample . MC500 Colorimeter 06-2010...

  • Page 16: Alkalinity-Total (Alkalinity-M, M-Value)

    Place the vial in the sample chamber making sure that marks are aligned. Zero accepted prepare Test Press TEST key. press TEST The result is shown in the display as total Alkalinity. MC500 Colorimeter 06-2010...
  • Page 17 0.02 0.056 0.07 *Carbonate hardness (reference = Hydrogen carbonate-anions) Example: 10 mg/l CaCO = 10 mg/l x 0.056 = 0.56 °dH 10 mg/l CaCO = 10 mg/l x 0.02 = 0.2 mmol/l CaCO °dH °eH °fH °aH MC500 Colorimeter 06-2010...

  • Page 18: Alkalinity-P (P-Value)

    7. Place the vial in the sample chamber making sure that the marks are aligned. Zero accepted prepare Test 8. Press TEST key. press TEST The result is shown in the display as Alkalinity-p. MC500 Colorimeter 06-2010...
  • Page 19 Case 2: Alkalinity-p > 0 and Alkalinity-m > 2p Hydrogen carbonate = m – 2p Carbonate = 2p Hydroxide = 0 Case 3: Alkalinity-p > 0 and Alkalinity-m < 2p Hydrogen carbonate = 0 Carbonate = 2m – 2p Hydroxide = 2p – m MC500 Colorimeter 06-2010...

  • Page 20: Aluminum With Tablets

    Test 9. Press TEST key. press TEST Wait for a reaction period of 5 minutes. Countdown 5:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l Aluminum. MC500 Colorimeter 06-2010...
  • Page 21 0.45 0.09 0.20 0.29 0.37 0.48 Example: If the result of Aluminum determination is 0.15 mg/l Al and the Fluoride concentration is known to be 0.4 mg/l F. the true concentration of Aluminum is 0.17 mg/l Al. MC500 Colorimeter 06-2010...

  • Page 22: Aluminum (Powder Pack)

    (this is the sample). 10. Close the vials tightly with the caps and swirl several times to mix the contents. Countdown 2 11. Press [ ] key. 5:00 start: Wait for a reaction period of 5 minutes. MC500 Colorimeter 06-2010...
  • Page 23 0.45 0.09 0.20 0.29 0.37 0.48 Example: If the result of Aluminum determination is 0.15 mg/l Al and the Fluoride concentration is known to be 0.4 mg/l F, the true concentration of Aluminum is 0.17 mg/l Al. MC500 Colorimeter 06-2010...

  • Page 24: Ammonia With Tablets

    Test 9. Press TEST key. press TEST Wait for a reaction period of 10 minutes. Countdown 10:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l Ammonium. MC500 Colorimeter 06-2010...
  • Page 25 Fill the test tube with the sample to the 10 ml mark and add one level spoonful of Conditioning Powder. Mix to dissolve, then continue as described in the test instructions. 5. Conversion: mg/l NH = mg/l N x 1.29 mg/l NH = mg/l N x 1.22 MC500 Colorimeter 06-2010...

  • Page 26: Ammonia (Powder Pack)

    12. Place the vial (the sample) in the sample chamber making sure that the marks are aligned. Zero accepted 13. Press TEST key. prepare Test press TEST The result is shown in the display in mg/l Ammonium. MC500 Colorimeter 06-2010...
  • Page 27 Glycine, Hydrazine, Less common interferences such as Hydrazine and Color, Turbidity Glycine will cause intensified colors in the prepared sample. Turbidity and color will give erroneous high values. Samples with severe interferences require distillation. MC500 Colorimeter 06-2010...

  • Page 28: Ammonia, Low Range (Lr)

    10. Place the vial (the sample) in the sample chamber making sure that the marks are l aligned. Zero accepted 11. Press TEST key. prepare Test press TEST The result is shown in the display in mg/l Ammonium. MC500 Colorimeter 06-2010...
  • Page 29 Determine the amount of total iron present in the water sample. To produce the blank add an iron standard solution with the same iron concentration to the vial (point 1) instead of deionized water 4. Conversion: mg/l NH = mg/l N x 1.29 mg/l NH = mg/l N x 1.22 MC500 Colorimeter 06-2010...

  • Page 30: Ammonia, High Range (Hr)

    10. Place the vial (the sample) in the sample chamber making sure that the marks are l aligned. Zero accepted 11. Press TEST key. prepare Test press TEST The result is shown in the display in mg/l Ammonium. MC500 Colorimeter 06-2010...
  • Page 31 Determine the amount of total iron present in the water sample. Add an iron standard solution with the same concentration to the vial (point 1) instead of deionized water to produce the blank. 4. Conversion: mg/l NH = mg/l N x 1.29 mg/l NH = mg/l N x 1.22 MC500 Colorimeter 06-2010...

  • Page 32: Boron

    Test 9. Press TEST key. press TEST Wait for a reaction period of 20 minutes. Countdown 20:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l Boron. MC500 Colorimeter 06-2010...
  • Page 33 2. The sample solution should have a pH value between 6 and 7. 3. Interferences are prevented by the presence of EDTA in the tablets. 4. The rate of color development depends on the temperature. The temperature of the sample must be 20°C ± 1°C. MC500 Colorimeter 06-2010...

  • Page 34: Bromine

    8. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted 9. Press TEST key. prepare Test press TEST The result is shown in the display in mg/l Bromine. MC500 Colorimeter 06-2010...
  • Page 35 0 mg/l. In this case, the water sample must be diluted with water free of Bromine. 10 ml of the diluted sample will be mixed with the reagent and the measurement repeated. Oxidizing agents such as Chlorine, Ozone etc. interfere as they react like Bromine. MC500 Colorimeter 06-2010...

  • Page 36: Chloramines, (Mono) And Free Ammonia

    Sample temperature in Reaction period in min °F / °C 41 / 5 50 / 10 60.8 / 16 68 / 20 73.4 / 23 77 / 25 MC500 Colorimeter 06-2010...
  • Page 37 Wait for a reaction period of 5 minutes. press TEST Countdown After the reaction period is finished the measurement 5:00 starts automatically. The result is shown in the display in mg/l Monochloramine. mg/l Cl mg/l NH mg/l N Notes: see previous page MC500 Colorimeter 06-2010...
  • Page 38 After the reaction period is finished the measurement 5:00 starts automatically. 11. Remove the vial from the sample chamber. 12. Add the contents of one Monochlor F RGT Powder Pack straight from the foil into the ammonia vial. MC500 Colorimeter 06-2010...
  • Page 39 (T2) is calculated. If T2 exceeds the range limit the following message is displayed: Cl + NH > 0.5 mg/l In this case the sample has to be diluted and the measurement repeated. 3. see also page 34 MC500 Colorimeter 06-2010...

  • Page 40: Chloride

    Test 9. Press TEST key. press TEST Wait for a reaction period of 2 minutes. Countdown After the reaction period is finished the measurement 2:00 starts automatically. The result is shown in the display in mg/l Chloride. MC500 Colorimeter 06-2010...
  • Page 41 3. Ions which also form deposits with Silver nitrate in acidic media, such as Bromides, Iodides and Thiocyanates, interfere with the analysis. 4. Highly alkaline water should - if necessary - be neutralized using Nitric acid before analysis. MC500 Colorimeter 06-2010...

  • Page 42: Chlorine

    Chlorine. >> free for the determination of free Chlorine. for the determination of total Chlorine. >> total Select the desired determination with the arrow keys [ ] and [ ]. Confirm with [ ] key. MC500 Colorimeter 06-2010...
  • Page 43 * it is not possible to give exactly values, because the development of turbidity depends on nature and ingredients of the sample. 7. If ??? is displayed at a differentiated test result see page 260. Oxidizing agents such as Bromine, Ozone etc. interfere as they react like Chlorine. MC500 Colorimeter 06-2010...

  • Page 44: Differentiated Determination (Free, Combined, Total)

    11. Add one DPD No. 3 tablet straight from the foil to the same water sample and crush the tablet using a clean stirring rod. 12. Close the vial tightly with the cap and swirl several times until the tablet is dissolved. MC500 Colorimeter 06-2010...
  • Page 45 After the reaction period is finished the measurement starts automatically. The result is shown in the display in: *,** mg/l free Cl mg/l free Chlorine *,** mg/l comb Cl mg/l combined Chlorine *,** mg/l total Cl mg/l total Chlorine Notes: See page 41. MC500 Colorimeter 06-2010...

  • Page 46: Free Chlorine

    8. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted prepare Test 9. Press TEST key. press TEST The result is shown in the display in mg/l free Chlorine. Notes: See page 41. MC500 Colorimeter 06-2010...

  • Page 47: Total Chlorine

    Wait for a reaction period of 2 minutes. press TEST Countdown 2:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l total Chlorine. Notes: See page 41. MC500 Colorimeter 06-2010...

  • Page 48: Chlorine With Liquid Reagent

    10. Remove the vial from the sample chamber. 11. Add 3 drops of DPD 3 solution to the same water sample. 12. Close the vial tightly with the cap and swirl several times to mix the contents. MC500 Colorimeter 06-2010...
  • Page 49 *,** mg/l total Cl mg/l total Chlorine Notes: 1. After use replace the bottle caps securely noting the color coding. 2. Store the reagent bottles in a cool, dry place ideally between 6°C and 10°C. 3. Also see page 41. MC500 Colorimeter 06-2010...

  • Page 50: Free Chlorine

    Notes (free and total Chlorine): 1. After use replace the bottle caps securely noting the color coding. 2. Store the reagent bottles in a cool, dry place ideally between 6°C and 10°C. 3. Also see page 41. MC500 Colorimeter 06-2010...

  • Page 51: Total Chlorine

    9. Press TEST key. press TEST Wait for a reaction period of 2 minutes. Countdown 2:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l total Chlorine. MC500 Colorimeter 06-2010...

  • Page 52: Differentiated Determination (Free, Combined, Total)

    10. Add the contents of one Chlorine TOTAL-DPD / F10 Powder Pack straight from the foil to the water sample. 11. Close the vial tightly with the cap and swirl several times to mix the contents (approx. 20 seconds). MC500 Colorimeter 06-2010...
  • Page 53 After the reaction period is finished the measurement starts automatically. The result is shown in the display in: *,** mg/l free Cl mg/l free Chlorine *,** mg/l comb. Cl mg/l combined Chlorine *,** mg/l total Cl mg/l total Chlorine Notes: See page 41. MC500 Colorimeter 06-2010...

  • Page 54: Free Chlorine

    7. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted prepare Test 8. Press TEST key. press TEST The result is shown in the display in mg/l free Chlorine. Notes: See page 41. MC500 Colorimeter 06-2010...

  • Page 55: Total Chlorine

    TEST Wait for a reaction period of 3 minutes. Countdown 3:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l total Chlorine. Notes: See page 41. MC500 Colorimeter 06-2010...

  • Page 56: Chlorine Dioxide

    >> with Cl of Chlorine. for the determination of Chlorine dioxide in the absence of >> without Cl Chlorine. Select the desired determination with the arrow keys [ ] and [ ]. Confirm with [ ] key. MC500 Colorimeter 06-2010...
  • Page 57 Chlorine dioxide. 10 ml of the diluted sample will be mixed with the reagent and the measurement repeated. 5. If ??? is displayed at a differentiated test result see page 260. Oxidizing agents such as Chlorine, Ozone etc. interfere as they react like Chlorine dioxide. MC500 Colorimeter 06-2010...

  • Page 58: In Presence Of Chlorine

    10. Close the vial tightly with the cap and swirl several times until the tablet is dissolved. 11. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted prepare T1 press TEST 12. Press TEST key. MC500 Colorimeter 06-2010...
  • Page 59 Chlorine dioxide in mg/l Chlorine, *,** mg/l ClO as Chlorine dioxide in mg/l ClO mg/l free Chlorine *,** mg/l free Cl mg/l combined Chlorine *,** mg/l comb. Cl mg/l total Chlorine *,** mg/l total Cl Notes: See next page. MC500 Colorimeter 06-2010...
  • Page 60 2. The total Chlorine result given includes the contribution by the Chlorine dioxide (as Chlorine) reading. For true total Chlorine value subtract the Chlorine dioxide (as Chlorine) reading from the quoted total Chlorine reading. 3. Also see page 55. MC500 Colorimeter 06-2010...

  • Page 61: In Absence Of Chlorine

    Test 9. Press TEST key. press TEST The result is shown in the display *,** mg/l ClO [Cl] as Chlorine dioxide in mg/l Chlorine, *,** mg/l ClO as Chlorine dioxide in mg/l ClO Notes: See page 55. MC500 Colorimeter 06-2010...

  • Page 62: Chlorine Hr (Kl)

    8. Place the vial in the sample chamber making sure that the marks are l aligned. Zero accepted prepare Test 9. Press TEST key. press TEST The result is shown in the display in mg/l Chlorine. MC500 Colorimeter 06-2010...
  • Page 63 1.1 Methods Notes: 1. Oxidizing agents interfere as they react like Chlorine. MC500 Colorimeter 06-2010...

  • Page 64: Cod, Low Range (Lr)

    9. Place the vial (the sample (Note 3, 4)) in the sample chamber making sure that the marks are l aligned. Zero accepted prepare Test 10. Press TEST key. press TEST The result is shown in the display in mg/l COD. MC500 Colorimeter 06-2010...
  • Page 65 5. Samples can be measured when the Chloride contents does not exceed 1000 mg/l. 6. Be aware that low readings can result if the water contains compounds that cannot be oxidized completely, as compared to the reference method. MC500 Colorimeter 06-2010...

  • Page 66: Cod, Middle Range (Mr)

    9. Place the vial (the sample (Note 3, 4)) in the sample chamber making sure that the marks are l aligned. Zero accepted 10. Press TEST key. prepare Test press TEST The result is shown in the display in mg/l COD. MC500 Colorimeter 06-2010...
  • Page 67 6. Be aware that low readings can result if the water contains compounds that cannot be oxidized completely, as compared to the reference method. 7. For samples under 100 mg/l COD it is recommended to repeat the test with the tube test for COD LR. MC500 Colorimeter 06-2010...

  • Page 68: Cod, High Range (Hr)

    9. Place the vial (the sample (Note 3, 4)) in the sample chamber making sure that the marks are l aligned. Zero accepted prepare Test 10. Press TEST key. press TEST The result is shown in the display in g/l COD. MC500 Colorimeter 06-2010...
  • Page 69 7. For samples under 1 g/l COD it is recommended to repeat the test with the test kit for COD MR or for samples under 0,1 g/l COD with the tube test COD LR. MC500 Colorimeter 06-2010...

  • Page 70: Color

    10 ml filtrated water sample (from Step B). 6. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted prepare Test 7. Press TEST key. press TEST The result is shown in the display in Pt-Co units (mg/l Pt). MC500 Colorimeter 06-2010...
  • Page 71 Do not stir the sample; avoid lengthy contact with the air. The sample may be stored in a dark place at a temperature of 39.2°F (4°C) for 24 hours. Before performing measurements, the water sample must be brought up to room temperature. MC500 Colorimeter 06-2010...

  • Page 72: Copper With Tablet

    Copper. >> total Select the desired determination with the arrow keys [ ] and [ ]. Confirm with [ ] key. Note: 1. If ??? is displayed at the diffentiated test result see page 260. MC500 Colorimeter 06-2010...

  • Page 73: Differentiated Determination (Free, Combined, Total)

    T2 13. Press TEST key. press TEST The result is shown in the display in: *,** mg/l free Cu mg/l free Copper *,** mg/l comb Cu mg/l combined Copper *,** mg/l total Cu mg/l total Copper MC500 Colorimeter 06-2010...

  • Page 74: Free Copper

    7. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted 8. Press TEST key. prepare Test press TEST The result is shown in the display in mg/l free Copper. MC500 Colorimeter 06-2010...

  • Page 75: Total Copper

    7. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted 8. Press TEST key. prepare Test press TEST The result is shown in the display in mg/l total Copper. MC500 Colorimeter 06-2010...

  • Page 76: Copper Pp

    Test 8. Press TEST key. press TEST Wait for a reaction period of 2 minutes. Countdown 2:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l Copper MC500 Colorimeter 06-2010...
  • Page 77 If a turbidity remains and turns black, silver interferences is likely. Add 10 drops of saturated Potassium chloride solution to 75 ml of water sample. Filtrate through a fine filter. Use 10 ml of the filtered water sample to perform test. MC500 Colorimeter 06-2010...

  • Page 78: Cyanide

    Test 10. Press TEST key. press TEST Wait for a reaction period of 10 minutes. Countdown 10:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l Cyanide. MC500 Colorimeter 06-2010...
  • Page 79 2. In the present of Thiocyanate, heavy metal complexes, colorants or aromatic amines, the cyanide must be separated out by distillation before analysis is performed. 3. Store the reagents in closed containers at a temperature of + 15°C to + 25°C. MC500 Colorimeter 06-2010...

  • Page 80: Cyanuric Acid

    (Note 2, 3). 7. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted 8. Press TEST key. prepare Test press TEST The result is shown in the display in mg/l Cyanuric acid. MC500 Colorimeter 06-2010...
  • Page 81 1. Use deionized water or tap water free of Cyanuric acid. 2. If Cyanuric acid is present a cloudy solution will result. 3. Dissolve the tablet completely (therefore swirl the vial approx. 1 minute). Undissolved particles of the tablet can cause too high results. MC500 Colorimeter 06-2010...

  • Page 82: Deha T

    Test 10. Press TEST key. press TEST Wait for a reaction period of 10 minutes. Countdown After the reaction period is finished the measurement 10:00 starts automatically. The result is shown in the display as DEHA. MC500 Colorimeter 06-2010...
  • Page 83 0.05 mg/l Manganese 0.8 mg/l Molybdenum 80 mg/l Nickel 0.8 mg/l Phosphate 10 mg/l Phosphonates 10 mg/l Sulfate 1000 mg/l Zinc 50 mg/l 6. There is an option to change the unit from mg/l to μg/l. mg/l µg/l MC500 Colorimeter 06-2010...

  • Page 84: Deha Pp

    10. Remove the vial from the sample chamber. 11. Place the vial (the sample) in the sample chamber making sure that the marks are aligned. Zero accepted prepare Test 12. Press TEST key. press TEST The result is shown in the display as DEHA. MC500 Colorimeter 06-2010...
  • Page 85 0.05 mg/l Manganese 0.8 mg/l Molybdenum 80 mg/l Nickel 0.8 mg/l Phosphate 10 mg/l Phosphonates 10 mg/l Sulfate 1000 mg/l Zinc 50 mg/l 7. There is an option to change the unit from mg/l to μg/l. mg/l µg/l MC500 Colorimeter 06-2010...

  • Page 86: Fluoride

    Zero accepted 7. Place the vial in the sample chamber making sure that prepare Test marks are aligned. press TEST Press TEST key. The result is shown in the display in mg/l Fluoride. MC500 Colorimeter 06-2010...
  • Page 87 2. 6. SPADNS reagent solution contains Arsenite. Chlorine concentrations up to 5 mg/l do not interfere. 7. Seawater and wastewater samples must be distilled. 8. It is convenient to use larger volume vials for this test. MC500 Colorimeter 06-2010...

  • Page 88: Hardness, Calcium (Using Calcheck Tablets)

    (5x) to mix the contents. 9. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted 10. Press TEST key. prepare Test press TEST The result is shown in the display as Calcium Hardness. MC500 Colorimeter 06-2010...
  • Page 89 3. This method was developed from a volumetric procedure for the determination of calcium. Due to undefined conditions, the deviations from the standardized method may be greater. 4. It is convenient to use larger volume vials for this test. CaCO °dH °eH °fH °aH MC500 Colorimeter 06-2010...

  • Page 90: Hardness, Calcium (Using Calico Tablets)

    Test 9. Press TEST key. press TEST Wait for a reaction period of 2 minutes. Countdown 2:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display as Calcium Hardness. MC500 Colorimeter 06-2010...
  • Page 91 5. The tolerance of the method is increasing with higher concentrations. When diluting samples, this should be taken in account, always measuring in the first third of the range. 6. Interferences: • Magnesium hardness up to 200 mg/l CaCO does not interfere. • Iron concentration above 10 mg/l may cause low results. • Zinc concentration above 5 mg/l may cause high results. CaCO °dH °eH °fH °aH MC500 Colorimeter 06-2010...

  • Page 92: Hardness, Total

    8. Press TEST key. prepare Test press TEST Wait for a reaction period of 5 minutes. Countdown 5:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display as total Hardness. MC500 Colorimeter 06-2010...
  • Page 93 2. Conversion table: mg/l CaCO °dH °fH °eH 1 mg/l CaCO ---- 0.056 0.10 0.07 1 °dH 17.8 ---- 1.78 1.25 1 °fH 10.0 0.56 ---- 0.70 1 °eH 14.3 0.80 1.43 ---- CaCO °dH °eH °fH °aH MC500 Colorimeter 06-2010...

  • Page 94: Hardness, Total Hr

    Test 8. Press TEST key. press TEST Wait for a reaction period of 5 minutes. Countdown 5:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display as total Hardness. MC500 Colorimeter 06-2010...
  • Page 95 2. Conversion table: mg/l CaCO °dH °fH °eH 1 mg/l CaCO ---- 0.056 0.10 0.07 1 °dH 17.8 ---- 1.78 1.25 1 °fH 10.0 0.56 ---- 0.70 1 °eH 14.3 0.80 1.43 ---- CaCO °dH °eH °fH °aH MC500 Colorimeter 06-2010...

  • Page 96: Hydrazine

    (Note 4). 9. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted 10. Press TEST key. prepare Test press TEST The result is shown in the display as Hydrazine. MC500 Colorimeter 06-2010...
  • Page 97 If the result is above the detection limit of 0.05 mg/l, you should use new reagents, as it could result in major result deviations. 6. There is an option to change the unit from mg/l to μg/l. mg/l µg/l MC500 Colorimeter 06-2010...

  • Page 98: Hydrazine With Liquid Reagent

    Test 11. Press TEST key. press TEST Wait for a reaction period of 12 minutes. Countdown 12:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display as Hydrazine. MC500 Colorimeter 06-2010...
  • Page 99 Note: at point 7 use the unprepared water sample. Principle: Hydrazine is oxidized by the household bleach. The interference by color will be eliminated by zeroing. 5. There is an option to change the unit from mg/L to μg/L. mg/l µg/l MC500 Colorimeter 06-2010...

  • Page 100: Hydrazine With Vacu-Vials

    8. Press TEST key. prepare Test press TEST Wait for a reaction period of 10 minutes. Countdown 10:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display as Hydrazine. MC500 Colorimeter 06-2010...
  • Page 101 MSDS is also available at www.chemetrics.com. 3. Vacu-vials is a registered trade mark of the company CHEMetrics, Inc. / Calverton, VA. ® U.S.A. 4. There is an option to change the unit from mg/l to μg/l. mg/l µg/l MC500 Colorimeter 06-2010...

  • Page 102: Hydrogen Peroxide

    9. Press TEST key. press TEST Wait for a reaction period of 2 minutes. Countdown 2:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l Hydrogen peroxide. MC500 Colorimeter 06-2010...
  • Page 103 10 mg/l. In this case, the water sample must be diluted with water free of Hydrogen peroxide. 10 ml of the diluted sample will be mixed with the reagent and the measurement repeated. Oxidizing agents such as Chlorine, Ozone etc. interfere as they react like Hydrogen peroxide. MC500 Colorimeter 06-2010...

  • Page 104: Iodine

    8. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted prepare Test 9. Press TEST key. press TEST The result is shown in the display in mg/l Iodine. MC500 Colorimeter 06-2010...
  • Page 105 1.1 Methods Notes: 1. Oxidizing reagents, such as Chlorine, Bromine, etc. interfere as they react like Iodine. MC500 Colorimeter 06-2010...

  • Page 106: Iron

    *Determination of all dissolved iron and most undissolved forms of iron; most undissolved iron oxides are recovered by the reagent. *This information refers to analysis of the water sample without digestion. Further information is found in the method notes. MC500 Colorimeter 06-2010...
  • Page 107 Therefore add 1 ml concentrated sulfuric acid and 1 ml concentrated nitric acid to 100 ml water sample and boil to approx. half volume. After cooling down proceed as described above. MC500 Colorimeter 06-2010...

  • Page 108: Iron With Tablet

    8. Press TEST key. prepare Test Wait for a reaction period of 5 minutes. press TEST Countdown 5:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l Iron. MC500 Colorimeter 06-2010...
  • Page 109 2. The IRON (II) LR tablet is used for differentiation – as described above – instead of the IRON LR tablet. 3. For the determination of total dissolved and undissolved iron, a digestion is required. An example is described on page 105. MC500 Colorimeter 06-2010...

  • Page 110: Iron (Powder Packs)

    8. Press TEST key. press TEST Wait for a reaction period of 3 minutes (Note 5). Countdown 3:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l Iron. MC500 Colorimeter 06-2010...
  • Page 111 3. Very strong alkaline or acidic water samples must be adjusted to a ph-Value between 3 and 5 before analysis. 4. Accuracy is not affected by undissolved powder. 5. Water samples containing visible rust should be allowed to react at least five minutes. MC500 Colorimeter 06-2010...

  • Page 112: Iron (Tptz) (Powder Packs)

    9. Place the vial (the sample) in the sample chamber making sure that the marks are aligned. Zero accepted 10. Press TEST key. prepare Test press TEST The result is shown in the display in mg/l Iron. MC500 Colorimeter 06-2010...
  • Page 113 Cadmium 4.0 mg/l Chromium 0.25 mg/l (3+) Chromium 1.2 mg/l (6+) Cobalt 0.05 mg/l Copper 0.6 mg/l Cyanide 2.8 mg/l Manganese 50 mg/l Mercury 0.4 mg/l Molybdenum 4.0 mg/l Nickel 1.0 mg/l Nitrite Ion 0.8 mg/l MC500 Colorimeter 06-2010...

  • Page 114: Mangenese With Tablet

    Test 9. Press TEST key. press TEST Wait for a reaction period of 5 minutes. Countdown 5:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l Manganese. MC500 Colorimeter 06-2010...
  • Page 115 1.1 Methods Note: KMnO MC500 Colorimeter 06-2010...

  • Page 116: Manganese Lr (Powder Packs)

    12. Place the vial (the sample) in the sample chamber making sure that the marks are aligned. Zero accepted prepare Test 13. Press TEST key. press TEST The result is shown in the display in mg/l Manganese. MC500 Colorimeter 06-2010...
  • Page 117 The turbidity should disappear after point 7. 4. Water samples containing more than 5 mg/l iron should be allowed to react at least 10 minutes. 5. Conversion: mg/l MnO = mg/l Mn x 2.17 KMnO MC500 Colorimeter 06-2010...

  • Page 118: Manganese Hr (Powder Packs)

    Test 10. Press TEST key. press TEST Wait for a reaction period of 2 minutes. Countdown 2:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l Manganese. MC500 Colorimeter 06-2010...
  • Page 119 Sodium hydroxide before test. Do not exceed pH 5, as manganese may precipitate. 3. Interferences: Interfering substance Interference level Calcium greater than 700 mg/l Chloride greater than 70 000 mg/l Iron greater than 5 mg/l Magnesium greater than 100 000 mg/l KMnO MC500 Colorimeter 06-2010...

  • Page 120: Molybdate With Tablet

    10. Close the vial tightly with the cap. 11. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted 12. Press TEST key. prepare Test press TEST The result is shown in the display in mg/l Molybdate / Molybdenum. MC500 Colorimeter 06-2010...
  • Page 121 2. Under test conditions (pH 3.8 – 3.9) iron does not interfere nor do other metals at levels likely to be found in industrial water systems. 3. Conversions: mg/l Mo = mg/l MoO x 0.6 mg/l Na = mg/l MoO x 1.3 MC500 Colorimeter 06-2010...

  • Page 122: Molybdate Hr (Powder Pack)

    Wait for a reaction period of 5 minutes. Zero accepted prepare Test After the reaction period is finished the measurement press TEST starts automatically. Countdown The result is shown in the display in mg/l Molybdate / 5:00 Molybdenum. MC500 Colorimeter 06-2010...
  • Page 123 5 minutes is increased. Thus, it is very important to perform the test procedure continuously. 4. Substances below may interfere when when present in concentrations above these limits: Aluminium 50 mg/l Chromium 1000 mg/l Iron 50 mg/l Nickel 50 mg/l Nitrite all levels MC500 Colorimeter 06-2010...

  • Page 124: Nitrate

    10. Place the vial (the sample) in the sample chamber making sure that the marks are l aligned. Zero accepted prepare Test 11. Press TEST key. press TEST The result is shown in the display in mg/l Nitrate. MC500 Colorimeter 06-2010...
  • Page 125 1.1 Methods Notes: 1. Some solids may not dissolve. 2. Conversion: mg/l NO = mg/l N x 4.43 MC500 Colorimeter 06-2010...

  • Page 126: Nitrite With Tablet

    Test 8. Press TEST key. press TEST Wait for a reaction period of 10 minutes. Countdown 10:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l Nitrite. MC500 Colorimeter 06-2010...
  • Page 127 Copper (II)-ions may cause lower test results as they accelerate the decomposition of the Diazonium salt. It is improbable that theses interfering ions will occur in such high concentrations that they cause significant reading errors. 2. Conversion: mg/l NO = mg/l N x 3.29 MC500 Colorimeter 06-2010...

  • Page 128: Nitrite Lr (Powder Pack)

    Test 8. Press TEST key. press TEST Wait for a reaction period of 20 minutes. Countdown 20:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l Nitrite. MC500 Colorimeter 06-2010...
  • Page 129 • In samples with very high concentrations of Nitrate (> 100 mg/L N) a small amount of Nitrite will be found. Such high levels of Nitrate appear to undergo a slight amount of reduction to Nitrite, either spontaneously or during the reaction time of the test. MC500 Colorimeter 06-2010...

  • Page 130: Nitrogen, Total Lr

    14. Add 2 ml of the digested, treated water sample to the other TN Acid LR/HR vial (this is the sample). 15. Close the vials with the caps and swirl the vials gently several times to mix the contents (10 x, Note 9). (CAUTION: Vials warm up). MC500 Colorimeter 06-2010...
  • Page 131 12. Application: for water, wastewater and seawater 13. Interferences: Interfering substances that resulted in a concentration change of 10%: Bromide more than 60 mg/l and Chloride more than 1000 mg/l produce positive interferences. TN = Total Nitrogen MC500 Colorimeter 06-2010...

  • Page 132: Nitrogen, Total Hr

    14. Add 2 ml of the digested, treated water sample to the other TN Acid LR/HR vial (this is the sample). 15. Close the vials with the caps and swirl the vials gently several times to mix the contents (10 x, Note 9). (CAUTION: Vials warm up). MC500 Colorimeter 06-2010...
  • Page 133 12. Application: for water, wastewater and seawater 13. Interferences: Interfering substances that resulted in a concentration change of 10%: Bromide more than 60 mg/l and Chloride more than 1000 mg/l produce positive interferences. TN = Total Nitrogen MC500 Colorimeter 06-2010...

  • Page 134: Oxygen, Active

    8. Press TEST key. press TEST Wait for a reaction period of 2 minutes. Countdown 2:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l active Oxygen. MC500 Colorimeter 06-2010...
  • Page 135 * Active Oxygen is a synonym for a common disinfectant (based on “Oxygen“) in Swimming Pool Treatment. 1. When preparing the sample, the lost of Oxygen, e.g. by pipetting or shaking, must be avoided. 2. The analysis must take place immediately after taking the sample. MC500 Colorimeter 06-2010...

  • Page 136: Oxygen, Dissolved

    Invert the vial several times. Dry the outside of the vial. 8. Place the Vacu-vial in the sample chamber. ® 9. Press TEST key. Zero accepted prepare Test press TEST The result is shown in the display in μg/l Oxygen. MC500 Colorimeter 06-2010...
  • Page 137 2. Read the original test instruction and the MSDS (delivered with the test) before performing the test. MSDS also at www.chemetrics.com available. 3. Vacu-vials is a registered trade mark of the company CHEMetrics, Inc. / Calverton, VA, ® U.S.A. MC500 Colorimeter 06-2010...

  • Page 138: Ozone

    Ozone in the presence of Chlorine. >> without Cl for the determination of Ozone in the absence of Chlorine. Select the desired method with the arrow keys [ ] and [ ]. Confirm with [ ] key. MC500 Colorimeter 06-2010...
  • Page 139 10 ml of the diluted sample will be mixed with the reagent and the measurement repeated. 6. If ??? is displayed at the diffentiated test result see page 260. Oxidizing agents such as Bromine, Chlorine etc. interfere as they react like Ozone. MC500 Colorimeter 06-2010...

  • Page 140: In Presence Of Chlorine

    Fill the vial with a few drops of the water sample. 11. Add one DPD No. 1 tablet and one DPD No. 3 tablet straight from the foil and crush the tablets using a clean stirring rod. MC500 Colorimeter 06-2010...
  • Page 141 TEST Countdown After the reaction period is finished the measurement 2:00 starts automatically. The result is shown in the display in: *,** mg/l O mg/l Ozone *,** mg/l total Cl mg/l total Chlorine Notes: See page 137. MC500 Colorimeter 06-2010...

  • Page 142: In Absence Of Chlorine

    9. Press TEST key. press TEST Wait for a reaction period of 2 minutes. Countdown 2:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l Ozone. Notes: See page 137. MC500 Colorimeter 06-2010...
  • Page 143 This page intentionally left blank MC500 Colorimeter 06-2010...

  • Page 144: Phmb (Biguanide)

    7. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted prepare Test 8. Press TEST key. press TEST The result is shown in the display in mg/l PHMB. MC500 Colorimeter 06-2010...
  • Page 145 Rinse vials and caps thoroughly with tap water followed by deionized water. 3. The test result is influenced by Hardness and Total Alkalinity. The calibration of this method was done using water of the following concentration: Ca-Hardness: 200 mg/l CaCO Total Alkalinity: 120 mg/l CaCO MC500 Colorimeter 06-2010...

  • Page 146: Phosphate

    Tube Test 0.02 – 1.6 mg/l P Determination of ortho-Phosphate ions + condensed, inorganic Phosphates Phosphate, total with Tube Test 0.02 – 1.1 mg/l P Determination of ortho-Phosphate ions + condensed, inorganic Phosphates + organically combined Phosphates MC500 Colorimeter 06-2010...
  • Page 147 10 mg/l Iron greater than 100 mg/l Nickel greater than 300 mg/l Silica (Silicium dioxide) greater than 50 mg/l Silicate greater than 10 mg/l Sulfide at any level Zinc greater than 80 mg/l Phosphate, ortho = Phosphorus, reactive MC500 Colorimeter 06-2010...

  • Page 148: Phosphate, Ortho Lr With Tablet

    9. Press TEST key. press TEST Wait for a reaction period of 10 minutes. Countdown 10:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l ortho- Phosphate. MC500 Colorimeter 06-2010...
  • Page 149 Higher concentrations of Cu, Ni, Cr (III), V (V) and W (VI) interfere due to their color. Silicates doe not interfere (masked by Citric acid in the tablets). 5. see also page 145 6. Conversion: mg/l P = mg/l PO x 0.33 mg/l P = mg/l PO x 0.75 MC500 Colorimeter 06-2010...

  • Page 150: Phosphate, Ortho Hr With Tablet

    9. Press TEST key. press TEST Wait for a reaction period of 10 minutes. Countdown After the reaction period is finished the measurement 10:00 starts automatically. The result is shown in the display in mg/l ortho- Phosphate. MC500 Colorimeter 06-2010...
  • Page 151 320 ”Phosphate LR, ortho with Tablet“. 2. Only ortho-Phosphate ions react. 3. see also page 145 4. Conversions: mg/l P = mg/l PO x 0.33 mg/l P = mg/l PO x 0.75 MC500 Colorimeter 06-2010...

  • Page 152: Phosphate, Ortho (Powder Packs)

    8. Press TEST key. press TEST Wait for a reaction period of 2 minutes. Countdown 2:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l ortho- Phosphate. MC500 Colorimeter 06-2010...
  • Page 153 1.1 Methods Notes: 1. The reagent does not dissolve completely. 2. See also page 145 3. Conversions: mg/l P = mg/l PO x 0.33 mg/l P = mg/l PO x 0.75 MC500 Colorimeter 06-2010...

  • Page 154: Phosphate, Ortho (Tube Test)

    8. Press TEST key. press TEST Wait for a reaction period of 2 minutes. Countdown After the reaction period is finished the measurement 2:00 starts automatically. The result is shown in the display in mg/l ortho- Phosphate. MC500 Colorimeter 06-2010...
  • Page 155 Notes: 1. Use a funnel to add the reagent. 2. The reagent does not dissolve completely. 3. See also page 145 4. Conversions: mg/l P = mg/l PO x 0.33 mg/l P = mg/l PO x 0.75 MC500 Colorimeter 06-2010...

  • Page 156: Phosphate 1, Ortho C

    8. Press TEST key. press TEST Wait for a reaction period of 5 minutes. Countdown 5:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l ortho- Phosphate. MC500 Colorimeter 06-2010...
  • Page 157 MSDS also at www.chemetrics.com available. 3. Vacu-vials is a registered trade mark of the company CHEMetrics, Inc. / Calverton, VA ® U.S.A. 4. Only ortho-Phosphate ions react. 5. Sulfide, Thiosulfate und Thiocyanate cause low test results. MC500 Colorimeter 06-2010...

  • Page 158: Phosphate 2, Ortho C

    10. Press TEST key. press TEST Wait for a reaction period of 3 minutes. Countdown 3:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l ortho- Phosphate. MC500 Colorimeter 06-2010...
  • Page 159 MSDS also at www.chemetrics.com available. 3. Vacu-vials is a registered trade mark of the company CHEMetrics, Inc. / Calverton, VA ® U.S.A. 4. Only ortho-Phosphate ions react. 5. Sulfide, Thiosulfate und Thiocyanate cause low test results. MC500 Colorimeter 06-2010...

  • Page 160: Phosphate, Hydrolysable (Tube Test)

    Test press TEST Wait for a reaction period of 2 minutes. After the reaction period is finished the measurement Countdown starts automatically. 2:00 The result is shown in the display in mg/l acid hydrolyz- able Phosphate. MC500 Colorimeter 06-2010...
  • Page 161 2. Use a funnel to add the reagent. 3. The reagent will not dissolve completely. 4. See page 145 for further information 5. Conversions: mg/l PO = mg/l P x 3.07 mg/l P = mg/l P x 2.29 MC500 Colorimeter 06-2010...

  • Page 162: Phosphate, Total (Tube Test)

    14. Press TEST key. press TEST Wait for a reaction period of 2 minutes. Countdown After the reaction period is finished the measurement 2:00 starts automatically. The result is shown in the display in mg/l total Phosphate. MC500 Colorimeter 06-2010...
  • Page 163 2. Use a funnel to add the reagent. 3. The reagent will not dissolve completely. 4. see also page 145 5. Conversions: mg/l PO = mg/l P x 3.07 mg/l P = mg/l P x 2.29 MC500 Colorimeter 06-2010...

  • Page 164: Phosphonates (Persulfate Uv Oxidation Method)

    11. Add the contents of one Phosphate Rgt. F10 Powder Pack straight from the foil into each vial (blank and sample). 12. Close the vials tightly with the cap and swirl gently several times (30 sec.). (Note 6) MC500 Colorimeter 06-2010...
  • Page 165 7. The given reaction time of 2 minutes refers to a water sample temperature of more than 15°C. At a sample temperature lower than 15 °C a reaction time of 4 minutes is required. Tables: see next page MC500 Colorimeter 06-2010...
  • Page 166 0 – 2.5 0 – 5.0 0 – 12.5 0 – 25 0 – 125 Table 2: Phosphonate type Conversion factor for active phosphonate PBTC 2.840 1.050 HEDPA 1.085 EDTMPA 1.148 HMDTMPA 1.295 DETPMPA 1.207 1.490 MC500 Colorimeter 06-2010...
  • Page 167 500 mg/l Silicate 100 mg/l Sulfate 2000 mg/l Sulfide interferes at all concentrations Sulfite 100 mg/l Thiourea 10 mg/l highly buffered samples or may exceed the buffering capacity of the extreme sample pH reagents and require sample pretreatment MC500 Colorimeter 06-2010...

  • Page 168: Ph-Value Lr With Tablet

    7. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted prepare Test 8. Press TEST key. press TEST The result is shown in the display as pH-value. MC500 Colorimeter 06-2010...
  • Page 169 1 molar 2 molar 3 molar – 0.26 – 0.33 – 0.31 The values of Parson and Douglas (1926) are based on the use of Clark and Lubs buffers. 1 Mol NaCl = 58.4 g/l = 5.8 % MC500 Colorimeter 06-2010...

  • Page 170: Ph-Value With Tablet

    7. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted 8. Press TEST key. prepare Test press TEST The result is shown in the display as pH-value. MC500 Colorimeter 06-2010...
  • Page 171 1 molar 2 molar 3 molar – 0.21 – 0.26 – 0.29 The values of Parson and Douglas (1926) are based on the use of Clark and Lubs buffers. 1 Mol NaCl = 58.4 g/l = 5.8 % MC500 Colorimeter 06-2010...

  • Page 172: Ph-Value With Liquid Reagent

    7. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted prepare TEST 8. Press TEST key. press Test The result is shown in the display as pH-value. MC500 Colorimeter 06-2010...
  • Page 173 (0.18 ml PHENOL RED solution is equivalent to 6 drops). 3. After use, replace the bottle cap securely. 4. Store the reagent in a cool, dry place ideally at between 6°C / 40°F and 10°C/ 50 °F. MC500 Colorimeter 06-2010...

  • Page 174: Ph-Value Hr With Tablet

    7. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted prepare TEST 8. Press TEST key. press Test The result is shown in the display as pH-value. MC500 Colorimeter 06-2010...
  • Page 175 1 molar 2 molar 3 molar – 0.22 – 0.29 – 0.34 The values of Parson and Douglas (1926) are based on the use of Clark and Lubs buffers. 1 Mol NaCl = 58.4 g/l = 5.8 % MC500 Colorimeter 06-2010...

  • Page 176: Potassium

    7. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted 8. Press TEST key. prepare Test press TEST The result is shown in the display in mg/l Potassium. MC500 Colorimeter 06-2010...
  • Page 177 1.1 Methods Notes: 1. If Potassium is present, a cloudy solution will result. MC500 Colorimeter 06-2010...

  • Page 178: Silica

    Test 12. Press TEST key. press TEST Wait for a reaction period of 1 minute. Countdown After the reaction period is finished the measurement 1:00 starts automatically. The result is shown in the display in mg/l Silica. MC500 Colorimeter 06-2010...
  • Page 179 2. Phosphate ions generally do not interfere under the given reaction conditions. 3. If Phosphate is known to be absent, the addition of the SILICA PR tablet may be omitted. 4. Conversion: mg/l Si = mg/l SiO x 0.47 MC500 Colorimeter 06-2010...

  • Page 180: Silica Lr Pp

    9. Add the contents of one LR Silica Amino Acid F F10 Powder Pack straight from the foil into the vial (the sample). 10. Close the vial tightly with the cap and swirl several times to mix the contents. MC500 Colorimeter 06-2010...
  • Page 181 A pre-treatment with Sodium Hydrogen Carbonate and then with Sulfuric Acid will make these forms reactive with Molybdate (pre-treatment is given in “Standard Methods for the Examination of Water and Wastewater” under “ Silica-Digestion with Sodium Bicarbonate”). MC500 Colorimeter 06-2010...

  • Page 182: Silica Hr Pp

    Test 13. Press TEST key. press TEST Wait for a reaction period of 2 minutes. Countdown 2:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l Silica. MC500 Colorimeter 06-2010...
  • Page 183 The nature of these forms is not known. A pre-treatment with Sodium Hydrogencarbonate and then with Sulfuric Acid will make these forms reactive to Molybdate (pre-treatment is given in “Standard Methods for the Examination of Water and Wastewater” under “ Silica-Digestion with Sodium Bicarbonate”). MC500 Colorimeter 06-2010...

  • Page 184: Sodium Hypochlorite

    6. Add one ACIDIFYING GP tablet straight from the foil to the same water sample and crush the tablet using a clean stirring rod. 7. Close the vial tightly with the cap and swirl several times until the tablets are dissolved. MC500 Colorimeter 06-2010...
  • Page 185 Refer to precautionary information on theSodium Hypochlorite label. 2. The tablets must be added in the correct sequence. 3. This method provides a fast, simple and portable test. By carefully following the procedure, an accuracy +/- 1 weight % can be obtained. MC500 Colorimeter 06-2010...

  • Page 186: Sulfate T

    7. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted 8. Press TEST key. prepare Test press TEST The result is shown in the display in mg/l Sulfate. MC500 Colorimeter 06-2010...
  • Page 187 1.1 Methods Notes: 1. If Sulfate is present, a cloudy solution will result. MC500 Colorimeter 06-2010...

  • Page 188: Sulfate (Powder Pack)

    8. Press TEST key. prepare Test Wait for a reaction period of 5 minutes. press TEST Countdown 5:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l Sulfate. MC500 Colorimeter 06-2010...
  • Page 189 1.1 Methods Note: 1. If Sulfate ions are present, a cloudy solution result. MC500 Colorimeter 06-2010...

  • Page 190: Sulfide

    Test 9. Press TEST key. press TEST Wait for a reaction period of 10 minutes. Countdown 10:00 After the reaction period is finished the measurement starts automatically. The result is shown in the display in mg/l Sulfide. MC500 Colorimeter 06-2010...
  • Page 191 3. To avoid loss of Sulfide ,collect the sample carefully with a minimum of aeration. It is essential to test the sample immediately after collection. 4. Ideally, the temperature of the water sample should be 20°C/ 70°F. Significant variation from this temperature can influence results. 5. Conversion: S = mg/l S x 1.06 MC500 Colorimeter 06-2010...

  • Page 192: Sulfite

    8. Press TEST key. prepare Test press TEST Wait for a reaction period of 5 minutes. Countdown After the reaction period is finished the measurement 5:00 starts automatically. The result is shown in the display in mg/l Sulfite. MC500 Colorimeter 06-2010...
  • Page 193 1.1 Methods Notes: MC500 Colorimeter 06-2010...

  • Page 194: Suspended Solids

    6. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted prepare Test 7. Press TEST key. press TEST The result is shown in the display in mg/l TSS (Total Suspended Solids). MC500 Colorimeter 06-2010...
  • Page 195 4. Collect water samples in clean plastic or glass bottles and analyse the water sample as soon as possible. It is possible to store the sample at 4°C for 7 days. Before measurement warm up the sample to the temperature at collection time. 5. Interferences: • Air bubbles interfere and can be removed by swirling the vial gently. • Color interferes if light is absorbed at 660 nm. MC500 Colorimeter 06-2010...

  • Page 196: Turbidity

    6. Close the vial tightly with the cap and swirl gently several times. 7. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted prepare Test 8. Press TEST key. press TEST The result is shown in the display in FAU. MC500 Colorimeter 06-2010...
  • Page 197 4. Color interferes if light is absorbed at 530 nm. For strong colored water samples a filtrated portion of the sample can be used for zeroing instead of the deionzed water. 5. Air bubbles interfere and can be removed using an ultrasonic bath. MC500 Colorimeter 06-2010...

  • Page 198: Urea

    11. Add one AMMONIA No. 2 tablet straight from the foil to the same water sample and mix to dissolve with a clean stirring rod. MC500 Colorimeter 06-2010...
  • Page 199 8. Before analysing seawater samples, a measuring spoon of Ammonia Conditioning Powder must be added to the sample and swirled to dissolve before AMMONIA No. 1 tablet is added. 9. Fill the vial with drops of the same size by holding the bottle vertically and squeeze slowly. MC500 Colorimeter 06-2010...

  • Page 200: Zinc

    9. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted 10. Press TEST key. press ZERO press TEST The result is shown in the display in mg/l Zinc. MC500 Colorimeter 06-2010...
  • Page 201 To dechlorinate, add one DECHLOR tablet to the water sample (point 1). Crush and mix to dissolve the tablet. Then add the COPPER / ZINC LR tablet (point 2) and continue with the test procedure as described above. MC500 Colorimeter 06-2010...

  • Page 202: Important Notes

    After use, replace the bottle caps securely noting color coding. Note recommendation for storage (e.g. cool and dry). Powder Packs: Vacu-vials of CHEMetrics: ® Vacu-vials should be stored dark and at room temperature. ® For further information see MSDS. MC500 Colorimeter 06-2010...

  • Page 203: Cleaning Vials And Accessories For Analysis

    4. The vials must be positioned in the sample chamber for zeroing and test with the mark on the vial aligned with the mark on the instrument. Correct position of the vial (Ø 24 mm): MC500 Colorimeter 06-2010...
  • Page 204 10. To avoid possible errors caused by stray-light do not use the instrument in bright sunlight. Correct filling of the vial: Insertion of the adapter: adapter 16 mm Ø adapter 13 mm Ø MC500 Colorimeter 06-2010...

  • Page 205: Sample Dilution Teqniques

    10 mg/l. Total volume = 100 ml + 5 ml = 105 ml Correction factor = 105 ml / 100 ml = 1.05 Corrected result = 10 mg/l x 1.05 = 10.5 mg/l MC500 Colorimeter 06-2010...
  • Page 206 MC500 Colorimeter 06-2010...

  • Page 207: Part 2 Instrument Manual

    Part 2 Instrument Manual MC500 Colorimeter 06-2010...

  • Page 208: Operation

    8. Replace the battery compartment cover. Check the seal ring (E) of the notch if it is tight-fitting 9. Tighten the screws carefully. CAUTION Dispose of used batteries in accordance with all federal, state and local regulations. MC500 Colorimeter 06-2010...

  • Page 209: Instrument (Explosion Drawing)

    (A) screws (C) notch (D) batteries CAUTION: To assure that the instrument water resistance is retained: seal ring (E) must be in position • battery compartment cover (B) must be attached with the four screws provided • MC500 Colorimeter 06-2010...

  • Page 210: Overview Of Function Keys

    Function key: description in the text if key available Confirming Menu of colorimeter settings and further functions Mode Moving the cursor up or down Storing of displayed test result Store Performing Zero Zero Performing Test Test Displaying date and time / user-countdown Decimal point MC500 Colorimeter 06-2010...

  • Page 211: Displaying Time And Date

    2 minutes, 0 seconds = [Shift] + [0][2][0][0]. Confirm with [ ] key. The display shows: Countdown 02:00 Start countdown with [ ] key. start: After countdown has finished the colorimeter reverts to the previous display automatically. MC500 Colorimeter 06-2010...

  • Page 212: Operation Mode

    100 Chlorine Line 2: Range 0.02-6 mg/l Cl Line 3: Kind of reagent Tablet 24 mm Line 4: Vial DPD No 1 Line 5-7: Used reagent DPD No 3 tube = reagent vial contained in tube test MC500 Colorimeter 06-2010...

  • Page 213: Chemical Species Information

    ZERO Prepare a clean vial as described in “Method“ and place the vial in the sample chamber making sure that the marks are aligned. Zero Press [ZERO] key. Zero accepted prepare Test The display shows: press TEST MC500 Colorimeter 06-2010...

  • Page 214: Performing Test

    In this case the operator is responsible for ensuring the necessary reaction period by himself. Non-compliance with reaction periods lead to incorrect test results. 2. The time remaining is displayed continuously. The beeper indicates the last 10 seconds. MC500 Colorimeter 06-2010...

  • Page 215: Changing Chemical Species

    [ ] directly. (The assignment for the Code-No. is then 0 automatically.) The entire data set is stored with date, time, Code-No., method and test result. The display shows: Stored! The test result is then shown again. MC500 Colorimeter 06-2010...

  • Page 216: Printing Results

    Test Zero accepted • Press [TEST] key prepare Test press TEST The display shows: Confirm with [TEST] key Test • Press [ZERO] key to perform a new zero calibration. Zero prepare Zero The display shows: press ZERO MC500 Colorimeter 06-2010...

  • Page 217: Selecting A New Method

    ZERO vial. Zero accepted The display shows: prepare Test press TEST Perform measurement of the sample. 500 mAbs The display shows e.g.: TIP: To ensure reaction times the User-Countdown may be helpful (chapter 2.2.3, page 209). MC500 Colorimeter 06-2010...

  • Page 218: Photometer Settings

    Displaying only results of a selected time period Stor., method Displaying only results of one selected method System-info Information about the instrument e.g. current software-version Temperature Selection of °C or °F for Langelier Mode 70 User calibration Storage user calibration MC500 Colorimeter 06-2010...

  • Page 219: Blank Because Of Technical Requirements Instrument Basic Settings 1

    Press [MODE], [Shift] + [1][0] keys. Mode Confirm with [ ] key. The display shows: <Language> Deutsch >> English Press arrow key [ ] or [ ] to select the required language Francais from the displayed list. Confirm with [ ] key. MC500 Colorimeter 06-2010...

  • Page 220: Setting Date And Time

    Enter hours and minutes 09-05-14 15:07 e.g.: 3.07 p.m. = [Shift] + [1][5][0][7] Confirm with [ ] key. Note: While conforming date and time with [ ] key the seconds are adjusted to zero automatically. MC500 Colorimeter 06-2010...
  • Page 221 The “user-countdown” is also available if the countdown is switched off. 2. If the countdown function is switched off, the operator is responsible for ensuring the necessary reaction period by himself. Non-compliance with reaction periods lead to incorrect test results. MC500 Colorimeter 06-2010...
  • Page 222 Press [Shift] + [1] key to switch the signal-beep on. Confirm with [ ] key. Note: In the case of methods with reaction periods, an acoustic signal still sounds during the last 10 seconds of the countdown even if the key-beep is switched off. MC500 Colorimeter 06-2010...

  • Page 223: Printing Of Stored Results

    Test No.: The display shows e.g.: After printing the photometer goes back to <Mode-Menu> automatically. Note: It is possible to cancel the entering by [ESC]. All stored data are printed out. See chapter 2.5.1 Print of data. MC500 Colorimeter 06-2010...
  • Page 224 After printing the photometer goes back to mode menu automatically. Note: It is possible to cancel the entering by [ESC]. If you want to print only results of one day enter the same date twice to characterize the period. MC500 Colorimeter 06-2010...
  • Page 225 It is possible to cancel the entering by [ESC]. If you want to print only results of one Code-Number enter the same Code-Number twice. If you want to print all results without Code-No. (Code-Nr. is 0) enter Zero [0] twice. MC500 Colorimeter 06-2010...
  • Page 226 Start: Press [ ] key and all stored results of the selected method cancel: ESC are printed. After printing the photometer goes back to mode menu automatically. Note: It is possible to cancel the entering by [ESC]. MC500 Colorimeter 06-2010...
  • Page 227 Finish with [ESC] key. Flow Control will be set to the selection displayed at “is“. Press [Shift] + [2] key to select ”Baud rate”. <Baud rate> The display shows: is: 19200 select: [ ] [ ] save: cancel: MC500 Colorimeter 06-2010...

  • Page 228: Recall / Delete Stored Results

    • Press arrow key [ ] to display the following test result. • Press arrow key [ ] to display the previous test result. no data If there are no test results in memory the display shows: MC500 Colorimeter 06-2010...
  • Page 229 Press [F2] key to print all selected results. • End with [ESC]. Note: It is possible to cancel the entering by [ESC]. If you want to recall only results of one day enter the same date twice to select the time period. MC500 Colorimeter 06-2010...
  • Page 230 It is possible to cancel the entering by [ESC]. If you want to recall only results of one Code-Number enter the same Code-Number twice. If you want to recall all results without Code-No. (Code-Nr. is 0) enter Zero [0] twice. MC500 Colorimeter 06-2010...
  • Page 231 Press [ ] key and all stored results of the selected 30 Alkalinity-tot method are displayed. Start: cancel: ESC print: F3 • Press [F3] key to print the displayed result. print all: F2 • Press [F2] key to print all selected results. • End with [ESC]. MC500 Colorimeter 06-2010...
  • Page 232 After pressing key [Shift] + [1] the following acknow- ledgment is displayed: Press [ ] key to delete. <Delete data> Delete data Do not delete: ESC NOTE / CAUTION! All stored test results will be deleted! or cancel without deleting data by pressing [ESC] key. MC500 Colorimeter 06-2010...

  • Page 233: Calibration

    7. Add 10 CALCIO H No. 2 tablets straight from the foil to the same water, crush the tablets using a clean stirring rod and dissolve the tablets completely. 8. Press [ ] key. Zero accepted Wait for a reaction period of 2 minutes. Countdown 2:00 start: MC500 Colorimeter 06-2010...
  • Page 234 Confirm with [ ] key. <Calibration> The display shows: 1: M191 Ca-Hardness 2 2: M191 0 Jus. Reset 3: M170 Fluoride L Press [Shift] + [2] keys. <Calibration> The display shows: M191 Calcium Hardness 2T Reset ? YES: 1, NO: 0 MC500 Colorimeter 06-2010...
  • Page 235 5. Add exactly 2 ml SPADNS reagent solution to the water sample. Caution: Vial is filled up to the top! 6. Close the vial tightly with the cap and swirl gently several times to mix the contents. MC500 Colorimeter 06-2010...
  • Page 236 (see Standard methods 20th, 1998, APHA, AWWA, WEF 4500 F D., S. 4-82). 2. As the test result is highly dependent on exact sample and reagent volumes, the sample and reagent volumes should always be metered by using a 10 ml resp. 2 ml volumetric pipette (class A). MC500 Colorimeter 06-2010...

  • Page 237: User Calibration

    0.5 – 1.5 mg/l Cl Chlorine L Calibration with basic test 100 Chlorine free Chlorine PP 0.5 –1 mg/l Cl Chlorine (KI) HR 70 – 150 mg/l Cl Chlorine dioxide Calibration with basic test 100 Chlorine free MC500 Colorimeter 06-2010...
  • Page 238 Nitrogen, total LR 10 mg/l N Nitrogen, total HR 50 – 100 mg/l N pH-Value LR 6.0 – 6.6 pH-Value T 7.6 – 8.0 pH-Value L 7.6 – 8.0 pH-Value HR 8.6 –9.0 PHMB 15 –30 mg/l MC500 Colorimeter 06-2010...
  • Page 239 Cancel user calibration by pressing [ESC] key. 0.02-6 mg/l Cl2 0.90 mg/l free Cl2 up: , down: The display shows: save: Now, the method name is displayed inverse and the test result is calculated with the new calibration factor. MC500 Colorimeter 06-2010...
  • Page 240 The display shows: 0.02-6 mg/l Cl2 clear user calibration? YES: 1, NO: 0 Press [Shift] + [1] key to delete user-calibration. Press [Shift] + [0] key to keep the valid user-calibration. The instrument goes back to Zero-query automatically. MC500 Colorimeter 06-2010...

  • Page 241: Lab Function

    Storage of test results is possible. In case of stored test results the display shows “Profi-Mode” additionally. The selected settings are kept by the photometer also after it was switched off. To change photometer setting a new setting is required. MC500 Colorimeter 06-2010...

  • Page 242: User Operations

    If only a few methods are required it is recommendable to perform Mode 62 first, followed by Mode 60. All user-Polynomials (1-25) and -Concentrations (1-10) are displayed in the method list, although they are not programmed by the user. Non-programmed user-methods can’t be activated! MC500 Colorimeter 06-2010...
  • Page 243 YES: 1, NO: 0 • Press [Shift] + [1] key to display only one method in the method selection list. • Press [Shift] + [0] key to keep the valid method selection list. The instrument goes back to mode-menu automatically. MC500 Colorimeter 06-2010...
  • Page 244 6: 660 nm Enter the required wavelength, e.g.: [Shift] + [2] for 560 nm. Press [ ] or [ ] keys to select the required unit. choose unit: >> mg/l mmol/l mAbs µg/l Confirm with [ ] key. MC500 Colorimeter 06-2010...
  • Page 245 S1 accepted Enter the concentration of the second standard; S2: +________ e.g.: [Shift] + [0][.][1] | ESC | F1 • One step back with [ESC]. • Press [F1] key to reset numerical input. Confirm with [ ] key. MC500 Colorimeter 06-2010...
  • Page 246 Entering a User-Polynomial: Press [MODE], [Shift] + [6][5] keys. Mode Confirm with [ ] key. The display shows: <User polynoms> choose no.: ____ (800-824) Enter a method-number in the range from 800 to 824, e.g.: [Shift] + [8][0][0] MC500 Colorimeter 06-2010...
  • Page 247 • Press [ ] or [ ] key to change between plus and minus Min mAbs: +________ sign. Max mAbs: +________ • Enter the values in Absorbance (mAbs) for the upper limit (Max) and the lower limit (Min). Confirm every input with [ ] key. MC500 Colorimeter 06-2010...
  • Page 248 Save all your polynomial data in a written form because in case of power outage (e.g. changing the battery) all polynomial data will be lost and must be entered again. You might want to use Mode 67 to transfer all polynomial data to a PC. MC500 Colorimeter 06-2010...
  • Page 249 There is displayed the query: M800 delete? YES: 1, NO: 0 • Press [Shift] + [1] key to delete the selected User-Method. • Press [Shift] + [0] key to keep the valid User-Method. The instrument goes back to mode menu automatically. MC500 Colorimeter 06-2010...
  • Page 250 The display shows: Start: Press [ ] key to print out the data (e.g. wavelength, unit, ...) of all stored User-Methods. M800 The display shows e.g.: M803 After data transfer the photometer goes back to mode menu automatically. MC500 Colorimeter 06-2010...
  • Page 251 There is displayed the query: YES: 1, NO: 0 • Press [Shift] + [1] key to start initialization. • Press [Shift] + [0] key to to cancel without initialization. The instrument goes back to mode menu automatically. MC500 Colorimeter 06-2010...

  • Page 252: Special Functions

    [ ] key. total dissol. solids The display shows: 0<=TDS<=6000 +_ _ _ _ Enter the value for TDS (Total Dissolved Solids) in the range between 0 und 6000 mg/l and confirm with [ ] key. MC500 Colorimeter 06-2010...
  • Page 253 Confirm with [ ] key. <temperature> The display shows: 1: °C 2: °F Press [Shift] + [1] key to select degrees Celsius. Press [Shift] + [2] key to select degrees Fahrenheit. The instrument goes back to mode menu automatically. MC500 Colorimeter 06-2010...

  • Page 254: Instrument Basic Settings 2

    , cancel: Esc Press arrow key [ ] to display the number of performed tests and free memory capacity. <System-Info> Number of Tests: free records left Finish with [ESC] key. cancel: Esc MC500 Colorimeter 06-2010...

  • Page 255: Data Transfer

    If the update procedure will be interrupted (eg. interruption of connection, LoBat., etc.) the instrument isn´t able to work (no display answer). The instrument will only be able to work again after a complete data transfer. RJ 45 connector MC500 Colorimeter 06-2010...
  • Page 256 MC500 Colorimeter 06-2010...

  • Page 257: Part 3 Shipping Contents

    Part 3 Contents & Delivery MC500 Colorimeter 06-2010...

  • Page 258: Unpacking

    If there is any damage or something is missing, please contact your local distributor immediately. 3.2 Delivery content Standard content of MC500: 1 Photometer in plastic case 4 batteries (size; Type AA) 1 Instruction manual...

  • Page 259: Technical Data

    5 – 40°C at max. 30 – 90% relative humidity (without condensation) Language options English, German, French, Spanish, Italian, Portuguese, Polish; further languages via Internet-Update Storage capaity 1000 data sets Subject to technical modification! * measured with standard solutions MC500 Colorimeter 06-2010...

  • Page 260: Abbreviations

    German DIN 38409 demand. Total Dissolved Solids Low Range Medium Range High Range Reagents of Chemetrics © Liquid reagent Powder (-reagent) Powder Pack Tablet Tube Test DEHA N,N-Diethylhydroxylamine Diethyl-p-phenylendiamine DTNB Ellmans reagent 1-(2-Pyridylazo)-2-napthol PDMAB Paradimethylaminobenzaldehyde PPST 3-(2-Pyridyl)-5,6-bis(4-phenylsulfonic acid)1,2,4-triazine TPTZ 2,4,6-Tri-(2-Pyridyl)-1,3,5-triazine MC500 Colorimeter 06-2010...

  • Page 261: Troubleshooting

    Underrange for a test result a user of higher/lower concentration not possible calibration is not possible Zero not Light absorption Refer to chapter 2.3.4 accepted is too great or too low Performing Zero Clean sample chamber. Repeat zeroing. MC500 Colorimeter 06-2010...
  • Page 262 The comb Cl instrument is not able to Overrange calculate the combined Chlorine. The test should be repeated with a diluted sample. Error absorbance calibration of Fluoride was not Repeat calibration e.g.: T2>T1 correct MC500 Colorimeter 06-2010...
  • Page 263 Mode 13 and/or switch the displayed. activated. Profi-Mode off with Mode 50. It seems that a method Method is not activated in Activate the required method is not available. the user method list. in the user method list with Mode 60. MC500 Colorimeter 06-2010...

  • Page 264: General Problems

    Orbeco-Hellige, Inc. 6456 Parkland Drive Sarasota, FL Tel.: 941.756.6410 Technical changes without notice Toll Free US: 800.922.5242 Printed in Germany 06/10 Rev. 2d Fax: 941.727.9654 Orbeco-Hellige is a registered ® service@orbeco.com trademark of the Orbeco-Hellige, Inc. www.orbeco.com...

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