Page 1
99 Washington Street Melrose, MA 02176 Phone 781-665-1400 Toll Free 1-800-517-8431 Visit us at www.TestEquipmentDepot.com Instruction Manual HI 83203 Multiparameter Bench Photometer for Aquaculture...
STANDARD METHODS ..............................45 ACCESSORIES ................................46 WARRANTY ..................................47 HANNA LITERATURE ................................ 47 All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.
The amount of reagent is precisely dosed to ensure maximum reproducibility. HI 83203 bench photometer can be connected to a PC via an USB cable. The optional HI 92000 Windows Compatible Software helps users manage all their results.
SPECIFICATIONS Life of the instrument Light Life Light Detector Silicon Photocell Environment 0 to 50°C (32 to 122°F); max 90% RH non-condensing Power Supply external 12 Vdc power adapter built-in rechargeable battery Dimensions 235 x 200 x 110 mm (9.2 x 7.87 x 4.33") Weight 0.9 Kg For specifications related to each method (e.g.
Page 5
The optical system of HI 83203 is based on special subminiature tungsten lamps and narrow-band interference filters to guarantee both high performance and reliable results.
FUNCTIONAL DESCRIPTION INSTRUMENT DESCRIPTION 1) Open Cuvette Lid 2) Indexing mark 3) Cuvette point 4) Liquid Crystal Display (LCD) 5) Splash proof keypad 6) ON/OFF power switch 7) Power input connector 8) USB connector...
KEYPAD DESCRIPTION The keypad contains 8 direct keys and 3 functional keys with the following functions: Press to perform the function displayed above it on the LCD. Press to exit the current screen. Press to access the select method menu. Press to move up in a menu or a help screen, to increment a set value, to access second level functions.
Page 8
(c) take out the syringe and clean the outside of the syringe tip. Be sure that no drops are hanging on the tip of the syringe, if so eliminate them. Then, keeping the syringe in vertical position above the cuvette, push the plunger down into the syringe until the lower edge of the seal is exactly on the 0.5 mL mark.
Page 9
USING CUVETTES • Proper mixing is very important for reproducibility of the measurements. The right way of mixing a cuvette is specified for each method in the related chapter. (a) invert the cuvette a couple of times or for a specified time: hold the cuvette in the vertical position. Turn the cuvette upside-down and wait for all of the solution to flow to the cap end, then return the cuvette to the upright vertical position and wait for all of the solution to flow to the cuvette bottom.
• In order to avoid reagent leaking and to obtain more accurate measurements, close the cuvette first with the supplied HDPE plastic stopper and then the black cap. • Each time the cuvette is used, the cap must be tightened to the same degree.
METHOD REFERENCE TABLE Method Method Page Method Method Page description description Ammonia MR Nitrite HR Ammonia LR Nitrite LR Free Chlorine Dissolved Oxygen Total Chlorine Copper HR Phosphate HR Copper LR Phosphate LR Nitrate OPERATIONAL GUIDE POWER CONNECTION AND BATTERY MANAGEMENT The meter can be powered from an AC/DC adapter (included) or from the built-in rechargeable battery.
Page 12
• Turn the instrument ON via the ON/OFF power switch. • The meter will perform an autodiagnostic test. During this test, the Hanna Instrument logo will appear on the LCD. After 5 seconds, if the test was successful, the last method used will appear on the display.
CHEMICAL FORM Chemical form conversion factors are pre-programmed into the instrument and are method specific. In order to view the displayed result in the desired chemical form press to access the second level functions and then press the Chem Frm key to toggle between the available chemical forms for the selected method. S E T U P S E T U P S E T U P...
Page 14
Date / Time This option is used to set the instrument’s date and time. Press the Modify key to change the date/time. Press the functional keys to highlight the value to be modified (year, month, day, hour, minute or second). Use the keys to change the value.
H E L P M O D E HELP MODE HI 83203 offers an interactive contextual help mode that assists the user at any time. To access the help screens press HELP. The instrument will display additional information related to the current screen.
AMMONIA MEDIUM RANGE SPECIFICATIONS Range 0.00 to 10.00 mg/L Resolution 0.01 mg/L Accuracy ±0.05 mg/L ±5% of reading at 25 °C Typical EMC ±0.01 mg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92 , Method Nessler method.
Page 17
• Remove the cuvette. • Add 4 drops of HI 93715A-0 First reagent (6 drops for seawater analysis). Replace the cap and mix the solution. • Add 4 drops of HI 93715B-0 Second reagent (10 drops for seawater analysis). Replace the cap and mix the solution. •...
AMMONIA LOW RANGE SPECIFICATIONS Range 0.00 to 3.00 mg/L Resolution 0.01 mg/L Accuracy ±0.04 mg/L ±4% of reading at 25 °C Typical EMC ±0.01 mg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92 , Method Nessler method.
Page 19
• Remove the cuvette. • Add 4 drops of HI 93700A-0 First reagent (6 drops for seawater analysis). Replace the cap and mix the solution. • Add 4 drops of HI 93700B-0 Second reagent (10 drops for seawater analysis). Replace the cap and mix the solution. •...
FREE CHLORINE FREE CHLORINE FREE CHLORINE FREE CHLORINE FREE CHLORINE FREE CHLORINE SPECIFICATIONS Range 0.00 to 2.50 mg/L Resolution 0.01 mg/L Accuracy ±0.03 mg/L ±3% of reading at 25 °C Typical EMC ±0.01 mg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the EPA DPD method 330.5.
Page 21
• Press the Zero key. The meter will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette. Powder reagents procedure • Add the content of one packet of HI 93701-0 DPD reagent. Replace the cap and shake gently for 20 seconds (or 2 minutes for seawater analysis).
Page 22
• Reinsert the cuvette into the instrument. • Press Read to start the reading. The instrument displays the results in mg/L of free chlorine. INTERFERENCES Interference may be caused by: Bromine, Iodine, Ozone, Oxidized forms of Chromium and Manganese. In case of water with hardness greater than 500 mg/L CaCO , shake the sample for approximately 2 minutes after adding the powder reagent.
TOTAL CHLORINE SPECIFICATIONS Range 0.00 to 3.50 mg/L Resolution 0.01 mg/L Accuracy ±0.03 mg/L ±3% of reading at 25 °C Typical EMC ±0.01 mg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the EPA DPD method 330.5. The reaction between the chlorine and the DPD reagent causes a pink tint in the sample.
Page 24
• Press the Zero key. The meter will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette. Powder reagents procedure • Add 1 packet of HI 93711-0 DPD reagent. Replace the cap and shake gently for 20 seconds (or 2 minutes for seawater analysis). •...
Page 25
• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 2 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading. • The instrument displays the results in mg/L of total chlorine. Note: free and total chlorine have to be measured separately with fresh unreacted samples following the related procedure if both values are requested.
COPPER HIGH RANGE SPECIFICATIONS Range 0.00 to 5.00 mg/L Resolution 0.01 mg/L Accuracy ±0.02 mg/L ±4% of reading at 25 °C Typical EMC ±0.01 mg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 575 nm Adaptation of the EPA method. The reaction between copper and the bicinchoninate Method reagent causes a purple tint in the sample.
Page 27
• Remove the cuvette. • Add the content of one packet of HI 93702-0 Bicinchoninate. Replace the cap and shake gently for about 15 seconds. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 45 seconds and press Read.
COPPER LOW RANGE SPECIFICATIONS Range 0 to 1000 μg/L Resolution 1 μg/L Accuracy ±10 μg/L ±5% of reading at 25 °C Typical EMC ±1 μg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 575 nm Adaptation of the EPA method. The reaction between copper and the bicinchoninate Method reagent causes a purple tint in the sample.
Page 29
• Remove the cuvette. • Add the content of one packet of HI 95747-0 Bicinchoninate. Replace the cap and shake gently for about 15 seconds. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 45 seconds and press Read.
NITRATE SPECIFICATIONS Range 0.0 to 30.0 mg/L Resolution 0.1 mg/L Accuracy ±0.5 mg/L ±10% of reading at 25 °C Typical EMC ±0.1 mg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the cadmium reduction method. The reaction between nitrate and the reagent causes an amber tint in the sample.
Page 31
• Replace the cap and immediately shake vigorously up and down for exactly 10 seconds. Continue to mix by inverting the cuvette gently for 50 seconds, while taking care not to induce air bubbles. Powder will not completely dissolve. Time and way of shaking could sensitively affect the measurement.
NITRITE HIGH RANGE SPECIFICATIONS Range 0 to 150 mg/L Resolution 1 mg/L Accuracy ±4 mg/L ±4% of reading at 25 °C Typical EMC ±1 mg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 575 nm Method Adaptation of the Ferrous Sulfate method. The reaction between nitrite and the reagent causes a greenish-brown tint in the sample.
Page 33
• Add the content of one packet of HI 93708-0 reagent. Replace the cap and shake gently until completely dissolved. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 10 minutes and press Read.
NITRITE LOW RANGE SPECIFICATIONS Range 0.00 to 1.15 mg/L Resolution 0.01 mg/L Accuracy ±0.06 mg/L ±4% of reading at 25 °C Typical EMC ±0.01 mg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the EPA Diazotization method 354.1 . The reaction between nitrite and the reagent causes a pink tint in the sample.
Page 35
• Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 6 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in mg/L of nitrite.
DISSOLVED OXYGEN SPECIFICATIONS Range 0.0 to 10.0 mg/L Resolution 0.1 mg/L Accuracy ±0.4 mg/L ±3% of reading at 25 °C Typical EMC ± 0.1 mg/L Deviation Light Source Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the Standard Methods for the Examination of Water and Wastewater, Method edition , Azide modified Winkler method.
Page 37
• Let the sample stand and the flocculent agent will start to settle. • After approximately 2 minutes, when the upper half of the bottle becomes limpid, add 10 drops of HI 93732C-0. • Replace the cap and invert the bottle until the settled flocculent dissolves completely. The sample is ready for measurement when it is yellow and completely limpid.
Page 38
SPECIFICATIONS Range 6.5 to 8.5 pH Resolution 0.1 pH Accuracy ±0.1 pH at 25 °C Typical EMC ±0.1 pH Deviation Light Source Tungsten lamp with narrow band interference filter @ 525 nm Method Adaptation of the Phenol Red method. The reaction with the reagent causes a yellow to red tint in the sample.
Page 39
• Remove the cuvette and add 5 drops of HI 93710-0 Phenol Red Indicator. Replace the cap and mix the solution. • Reinsert the cuvette into the instrument. • Press the Read key to start the reading. The instrument displays the pH value.
PHOSPHATE HIGH RANGE SPECIFICATIONS 0.0 to 30.0 mg/L Range Resolution 0.1 mg/L Accuracy ±1 mg/L ±4% of reading at 25 °C Typical EMC Dev. ±0.1 mg/L Light Source Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the Standard Methods for the Examination of Water and Wastewater, Method edition, Amino Acid method.
Page 41
• Add the content of one packet of HI 93717B-0 Phosphate HR Reagent B to the cuvette. Replace the cap and shake gently until completely dissolved. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 5 minutes and press Read.
PHOSPHATE LOW RANGE SPECIFICATIONS Range 0.00 to 2.50 mg/L Resolution 0.01 mg/L Accuracy ±0.04 mg/L ±4% of reading at 25 °C Typical EMC Dev. ±0.01 mg/L Tungsten lamp with narrow band interference filter @ 610 nm Light Source Method Adaptation of the Ascorbic Acid method. The reaction between phosphate and the reagent causes a blue tint in the sample.
Page 43
• Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 3 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in mg/L of phosphate (PO ¯).
ERRORS AND WARNINGS The instrument shows clear warning messages when erroneous conditions appear and when measured values are outside the expected range. These messages are described below. No Light: The light source is not functioning properly. Light Leak: There is an excess amount of ambient light reaching the detector.
DATA MANAGEMENT The analyzed data can be managed using Hanna’s product HI 92000, Windows Compatible Software. ® STANDARD METHODS Description Range Method Ammonia MR 0.00 to 10.00 mg / L Nessler Ammonia LR 0.00 to 3.00 mg / L Nessler Chlorine, Free 0.00 to 2.50 mg / L...
ACCESSORIES REAGENT SETS HI 93700-01 100 ammonia LR tests HI 93700-03 300 ammonia LR tests HI 93701-01 100 free chlorine tests (powder) HI 93701-03 300 free chlorine tests (powder) HI 93701-F 300 free chlorine tests (liquid) HI 93701-T 300 total chlorine tests (liquid) HI 93702-01 100 copper HR tests HI 93702-03 300 copper HR tests HI 93707-01 100 nitrite LR tests...
If the repair is not covered by the warranty, you will be notified of the charges incurred. If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization Number from the Customer Service Department and then send it with shipment costs prepaid. When shipping any instrument, make sure it is properly packaged for complete protection.
Need help?
Do you have a question about the HI 83203 and is the answer not in the manual?
Questions and answers
ik heb een photometer HI83203 scherm meldt ERno 12 c err contact technical support kun je mij helpen aub
@firma mps