FujiFilm FLA-8000 Operation Manual
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FujiFilm FLA-8000 Operation Manual

FujiFilm FLA-8000 Operation Manual

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FLA-8000
OPERATION MANUAL
Version 3
2002. Jan. Version 3.0

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Summary of Contents for FujiFilm FLA-8000

  • Page 1 FLA-8000 OPERATION MANUAL Version 3 2002. Jan. Version 3.0...

  • Page 3: Table Of Contents

    Contents 2002. Jan. Verson 3.0 Contents Part 1 PRECAUTIONS ..................3 1. Grounding Safety ....................... 5 Purposes of grounding ..........................5 Grounding work ............................5 2. Laser Safety........................6 Lasers used ..............................6 Applicable safety standards ........................6 Laser safety ..............................6 Instrument cover ............................
  • Page 4 Part 9 Daily Maintenance ................105 1. Maintenance of SHG Laser ................... 106 Part 10 Troubleshooting ................107 Troubles in FLA-8000 body ....................108 Countermeasures according to warning and error messages ........111 Other Troubles ........................127 Part 11 Specifications .................. 129 Major Specifications ......................

  • Page 5: Part 1 Precautions

    2002. Jan. Verson 3.0 Part PRECAUTIONS...
  • Page 6 2002. Jan. Verson 3.0 This section describes the matters that require special attention to the safe use of the FLA-8000. This section shows the general precautions for using the FLA-8000, some of which are also described in other sections. CAUTION Do not place a vase, flowerpot or glass containing water in it or metallic object on this instrument.

  • Page 7: Grounding Safety

    1. Grounding Safety 2002. Jan. Verson 3.0 1. Grounding Safety Purposes of grounding It is quite important to ground an electric or electronic instrument. The purposes of grounding are as shown below: (1) Preventing damages to the instrument caused by charging of the metallic casing originating from deterioration or defects of insulating material of the electrical circuits of the instrument.

  • Page 8: Laser Safety

    2. Laser Safety 2002. Jan. Verson 3.0 2. Laser Safety The FLA-8000 uses the following three lasers: Lasers used (1) LD laser, class IIIb, wavelength of 635 nm, maximum power of 20 mW (CW) (2) SHG laser, class IIIb, wavelength of 532 nm, maximum power of 5.5 mW (CW)
  • Page 9 2002. Jan. Verson 3.0...

  • Page 10: High-Voltage Safety

    3. High-Voltage Safety / 4. Regulations and Standards 2002. Jan. Verson 3.0 3. High-Voltage Safety This instrument employs a high-voltage power supply for photo-multipliers (PMTs). However, the user is completely free from the possibility of touching the high-volt- age power supply, provided the user carries out operation (including user mainte- nance) of the instrument properly.

  • Page 11: Radiation Safety

    Such pollution is greatly influenced by the sample condition, and it is quite difficult to foresee the degree of pollution. Radioisotope pollution may harm the FLA-8000 body due to improper operation or an unexpected trouble. The performances of the FLA-8000 will not be deteriorated even if it is polluted by radioisotope.

  • Page 12: Radiation Hazard Prevention Related To Radioisotope Pollution

    6. Radiation Hazard Prevention Related to Radioisotope Pollution 2002. Jan. Verson 3.0 6. Radiation Hazard Prevention Related to Radioisotope Pollu- tion Article 1, paragraph 1 of the law enforcement rules (Prime Minister's Office ordi- nance No.56) for prevention of radiation hazards due to radioisotope and so forth Controlled area defines the controlled area as "a place where the dose equivalent related to exter- nal radiation exceeds the dose equivalent determined by the Director General of...

  • Page 13: Radioisotope (Ri) Pollution

    7. Radioisotope (RI) Pollution 2002. Jan. Verson 3.0 7. Radioisotope (RI) Pollution CAUTION Follow the instructions of your radiation administrator if radio isotope pollution occurs. This instrument is capable of reading IPs. The IP surface may be polluted by RI pollution of IP radioisotope (RI), depending on the sample conditions, if a sample is in direct con- tact with the IP surface.

  • Page 14: Pollution Of Instrument Body

    8. Maintenance of SHG Laser / 9. Installation Environment / 10. Precaution for Disposal 2002. Jan. Verson 3.0 The IP surface may be polluted by radioisotope. Therefore, RI pollution may ex- Pollution of instrument pand to the inside of the instrument in the case of improper operation. body It is necessary to use the instrument properly in order to prevent expansion of RI pollution (to the inside of the instrument), though the performances of the instru-...

  • Page 15: Other Instructions

    11. Other Instructions 2002. Jan. Verson 3.0 11. Other Instruc- tions CAUTION Slide carrier The slide carrier weighs 420 g. If it drops onto your feet, your feet will be injured. The slide carrier consists of precision parts. Handle it with great care.

  • Page 16: Gel Carrier

    11. Other Instructions 2002. Jan. Verson 3.0 Gel Carrier CAUTION The gel carrier weighs 460 g. The gel carrier has built-in glass. Be very careful not to drop it. Never put any objects other than samples on the glass or apply pressure to the glass.

  • Page 17: Ip Carrier

    11. Other Instructions 2002. Jan. Verson 3.0 IP Carrier CAUTION The IP carrier weighs 370 g. If it drops onto your feet, your feet will be injured. The IP carrier becomes unusable if it drops and deforms or its ends are lost. IP Carrier...
  • Page 18 2002. Jan. Verson 3.0...

  • Page 19: Part 2 System Configuration

    2002. Jan. Verson 3.0 Part SYSTEM CONFIGURATION...

  • Page 20: Features Of Instrument

    In addition, it may read image plates (IPs) originally developed as radiation energy sensors by Fuji Photo Film Co., Ltd. After reading an IP, the FLA-8000 emits beams onto it uniformly inside the eraser to erase the after-image. It is possible to use IPs repeatedly by erasing after- images.

  • Page 21: Standard System Configuration

    2. Standard System Configuration 2002. Jan. Verson 3.0 2. Standard System The standard components of this instrument are as shown below. 1. Instrument Body Configuration The instrument body reads IPs or slide glass, gel, etc. dyed with fluorescent pigments and transfers image data immediately to the analyzer unit. (*Hereinafter, the instrument body is merely referred to as the body in this manual.) Body...
  • Page 22 2. Standard System Configuration 2002. Jan. Verson 3.0 4. Gel Carrier Gel Carrier 5. IP Carrier IP Carrier 6. IP Filter The IP filter is used to read IP. Take the optional filter block out of the instrument, remove the shielding plate from the block with the jig included in the accessories, mount the IP filter, and set the folder to the instrument.

  • Page 23: Part 3 Switching On And Off

    2002. Jan. Verson 3.0 Part Switching On and Off...
  • Page 24 (closed) securely, the SCAN lamp on the FLA-8000 blinks and a buzzer sounds. 1. Push the "I" side of the power switch on the right side panel of the FLA-8000 as shown below. 2. When the FLA-8000 is switched on, the indicator lamps indicate its conditions as shown below.

  • Page 25: Part 4 Scanning Slide Glass Samples

    2002. Jan. Verson 3.0 Part Scanning Slide Glass Samples...
  • Page 26 2. Switch on the PC. 3. Make sure that warming-up is completed and the FLA-8000 is ready. Activate the FLA-8000 Image Reader from the Startup menu or using the shortcut key. 4. The main window of the FLA-8000 Image Reader is displayed.
  • Page 27 2-2. Push slide glass with the scanning side (with a sample) upside fully to the very end through the opening of the slide glass carrier. In an image scanned by the FLA-8000, the slide numbers on the slide glass Note: carrier are displayed in the upper part of the monitor screen.
  • Page 28 Scanning Slide Glass Samples (Carrier Set Mode) 2002. Jan. Verson 3.0 2-3. Open the sample setting door. 2-4. Open the carrier locking cover.
  • Page 29 2-5. Set the slide glass carrier. Hold the handle in the center of the carrier, and set the carrier so that the poles on the FLA-8000 are engaged with the holes in the carrier. 2-6. Close the carrier locking cover firmly.
  • Page 30 Scanning Slide Glass Samples (Carrier Set Mode) 2002. Jan. Verson 3.0 2-7. Close the sample setting door. 3. Go back to the PC, and click on Setting in the PC window. (On the right of Carrier Set in the upper part of the window or lower left of the window) 4.
  • Page 31 Scanning Slide Glass Samples (Setting Mode) 2002. Jan. Verson 3.0 Setting Mode 1. The following shows the details of the scanning conditions. 1-1. Resolution ( Resolution: Select a pixel scanning size. The scanning time becomes longer in inverse proportion to the pixel scanning size. 1-2.
  • Page 32 Scanning Slide Glass Samples (Setting Mode) 2002. Jan. Verson 3.0 Note: It is impossible to set the laser conditions independently for several pieces of glass slide in one-time scanning. All pieces of slide glass are scanned in the laser conditions displayed before clicking the Scan button. You may set the filter and high voltage conditions independently for each piece of slide glass.
  • Page 33 Scanning Slide Glass Samples (Setting Mode) 2002. Jan. Verson 3.0 a) Inputting numeric values Input numeric values in the boxes shown below directly. (Integers only) b) Dragging the mouse The white area is the scannable area, and the red frame area is the scan- ning range.
  • Page 34 Scanning Slide Glass Samples (Setting Mode) 2002. Jan. Verson 3.0 1-5. Selecting Confocal Mode or Non-Confocal Mode It is possible to select the confocal mode or non-confocal mode when read- ing slide glass. Confocal Mode ..... Fluorescent light from dust or foreign matters on slide glass and background components in the slide glass may be reduced in the confocal mode.
  • Page 35 In the Preview mode, the pixel size is larger and the reading speed is higher than in the final reading. 2-3. If scanning with three lasers is specified above, for example, the FLA-8000 scans the sample preliminary with these lasers three times in all as shown...
  • Page 36 Scanning Slide Glass Samples (Setting Mode) 2002. Jan. Verson 3.0 When scanning two pieces of slide glass using three lasers, the FLA-8000 pre-scans the sample with these lasers twice each, amounting to six times in all. 2-4 On completion of PreView, the window shown below appears.
  • Page 37 Scanning Slide Glass Samples (Setting Mode) 2002. Jan. Verson 3.0 2-5. Select a contrast scale to be applied to the PreView images. You may change the contrast scale as you like in order to observe the PreView images more clearly. Select a proper scale in the pull-down menu. 2-6.
  • Page 38 Number of gradations and dynamic range Note: The number of gradations and dynamic range of the FLA-8000 are fixed to the values shown below, regardless of the sample types. Number of gradations: Sixteen bits...
  • Page 39 Scanning Slide Glass Samples (Scan Mode) 2002. Jan. Verson 3.0 4. Click the Scan icon in the upper part of the Setting window or the Scan button in the lower right of the window to start final scanning. Scan Mode 1.
  • Page 40 Scanning Slide Glass Samples (Scan Mode) 2002. Jan. Verson 3.0 Cascade ....Displays overlaid images. Tile Horizontal ... Displays images horizontally.
  • Page 41 Scanning Slide Glass Samples (Scan Mode) 2002. Jan. Verson 3.0 Tile Vertical ....Displays images vertically. Arrange Icons ... Windows illustrated as title bars are arranged.
  • Page 42 Scanning Slide Glass Samples (Scan Mode) 2002. Jan. Verson 3.0 3. When Catalog is selected on the Window menu, the minimized catalog window is enlarged and displayed in the forefront. 4. Change images scanned by three types of lasers in the slide glass window as shown below.
  • Page 43 Scanning Slide Glass Samples (Scan Mode) 2002. Jan. Verson 3.0 Click on the mark in the upper part of the window to change the images as shown below..... Image scanned by the red 635 nm laser Green ....Image scanned by the green 532 nm laser Blue ....
  • Page 44 Scanning Slide Glass Samples (Scan Mode) 2002. Jan. Verson 3.0 ...... The red frame of the small image corresponds to the part displayed in the image window...... To enlarge a displayed image, click the button, and click the mouse button in a position to be enlarged in the image window.....
  • Page 45 Scanning Slide Glass Samples (Save Mode) 2002. Jan. Verson 3.0 If you want to change the gradation of displayed images, select a scale in the pull-down menu and adjust the gradation with the adjuster bar on the histogram or by dragging the button below the histogram. 6.
  • Page 46 Scanning Slide Glass Samples (Scan Mode) 2002. Jan. Verson 3.0 It is possible to give names to image files automatically in a certain rule. (Serial numbers are added to the end of image file names.) Each file name consists of the characters shown in the following example, in principle.
  • Page 47 Scanning Slide Glass Samples (Save Mode) 2002. Jan. Verson 3.0 When Yes (continuative) and suffix type of 1, 2, 3, 4, 5 and so forth are selected and the latest scanned image is stored in the file "xxx-6", for example, the file name "xxx-7"...
  • Page 48 Scanning Slide Glass Samples (Scan Mode) 2002. Jan. Verson 3.0 You may select one of the following two file formats. Img/Inf ......... This is the standard file format of the BAS/FLA series. Each file of this format consists of a luster file (xxx.img) and an information file (xxx.inf).
  • Page 49 Scanning Slide Glass Samples (Save Mode) 2002. Jan. Verson 3.0 The box below Root Folder shows where the folder for saving the images resides. If you want to change the folder, click the button and select an intended folder. 4. Inputting a comment You may input a comment in the Comment box below the Name box.
  • Page 50 2002. Jan. Verson 3.0...

  • Page 51: Part 5 Usage Of Ips

    2002. Jan. Verson 3.0 Part Usage of IPs...

  • Page 52: Usage Of Ips

    * Erasure You may reuse a general-purpose IP by erasing an after-image. 1.2 Available IPs The FLA-8000 may use the BAS-SR 0813 (85 mm x 127 mm) only at the time when this operation manual is prepared. * Use conditions It is necessary to stick a non-curled IP to the carrier free from separation.

  • Page 53: Instructions On Handling Ips

    1. Usage of IPs 2002. Jan. Verson 3.0 * General handling 1.4 Instructions on While handling IPs, always wear gloves to maintain the quality of the IPs. Be handling IPs very careful not to bend, damage or soil them. Do not handle them in a dusty place. * Cleaning General-purpose IPs: Clean the back surfaces of IPs with proper cleaning material such as KIMWIPE,...

  • Page 54: Precaution Before Exposure

    IP carrier into the FLA-8000. Setting the exposure time The highest image quality is guaranteed when an IP is scanned by the FLA-8000 immediately after it is exposed. Therefore, it is important to set the exposure start time and exposure time so that exposure ends just before scanning starts.

  • Page 55: Erasing Ips

    1. Usage of IPs 2002. Jan. Verson 3.0 Erase an IP sufficiently before exposing it. 1.6 Erasing IPs Note: If an IP is exposed excessively, it may not be erased completely. In such a case, scan it once by way of trial to check if it is erased completely. Erasing IPs with IP Refer to the manual of the IP eraser.

  • Page 56: Exposing And Scanning Ips

    User Tip: If the sample is smaller than the IP, put the sample to the side of the IP scanned first (i.e., left side when the IP is set in the FLA-8000) and expose it. Partial exposure reduces the scanning time.
  • Page 57 2. Exposing and Scanning IPs 2002. Jan. Verson 3.0 4 Close the cassette cover. (Close it firmly until a click sound is heard.) Do not have any IP corners be caught by the cassette cover. Note: Be very careful not to apply an impact to the cassette. An impact applied to the cassette may shift the sample and IP.
  • Page 58 2002. Jan. Verson 3.0...

  • Page 59: Part 6 Scanning Ips

    2002. Jan. Verson 3.0 Part Scanning IPs...
  • Page 60 1. Switch on the FLA-8000 and peripheral devices. 2. Switch on the PC. 3. Make sure that the FLA-8000 has warmed up and is ready for operation. Activate the FLA-8000 Image Reader from the Startup menu or using the shortcut key.
  • Page 61 Scanning IPs (Carrier Set Mode) 2002. Jan. Verson 3.0 4. The main window of the FLA-8000 Image Reader is displayed. Carrier Set Mode 1. Select the IP using Select Sample in the Carrier Set mode. Click on the IP icon or the radio button on the left of the IP icon to select the IP.
  • Page 62 Scanning IPs (Carrier Set Mode) 2002. Jan. Verson 3.0 2-2. Put the IP cassette with an exposed IP on the side of the IP carrier. 2-3. Turn down the room lighting (below 20 luxes). (Keep the lighting turned down until the IP carrier is set and the main cover is closed.) 2-4.
  • Page 63 Scanning IPs (Carrier Set Mode) 2002. Jan. Verson 3.0 The following figure shows the relationship between the IP setting position and Note: the image position on the monitor. When the IP is viewed from the back as shown below, scanning starts at the upper left corner. Back surface of the IP carrier 2-5.
  • Page 64 2-7. Set the IP carrier. Set the IP carrier so that the large hole for removing the IP in the carrier is located on the front side and the poles on the FLA-8000 are engaged with the holes in the carrier.
  • Page 65 2-8. Close the carrier locking cover firmly. Close the carrier locking cover so that the poles on both sides of the cover are securely engaged with the pole holds on the FLA-8000. (The pole holds are located on the right and left.)
  • Page 66 Scanning IPs (Setting Mode) 2002. Jan. Verson 3.0 3. Go back to the PC, and click on Setting in the PC window. (On the right of Carrier Set in the upper part of the window or lower left of the window) 4.
  • Page 67 * Filter and high voltage conditions are not displayed on the screen. Note: Filters for scanning an IP Be sure to set the optional FUJI B390 filters for scanning IPs in the FLA-8000 before scanning an IP. The FLA-8000 Image Reader does not automatically check whether the B390 filters are set.
  • Page 68 Scanning IPs (Setting Mode) 2002. Jan. Verson 3.0 Drag the red frame area with the mouse to vary the scanning range. As the red frame area changes, the numeric values in the Area boxes also change. 2. Other items in the Setting mode 2-1.
  • Page 69 Scanning IPs (Setting Mode) 2002. Jan. Verson 3.0 2-3. Saving and loading the scanning conditions You may save and load (recall) the scanning conditions. Saving the scanning conditions: Select "Save Condition" in the pull-down menu of the File menu. A dialog box opens. Input an intended name in the dialog box and save the file.
  • Page 70 Scanning IPs (Scan Mode) 2002. Jan. Verson 3.0 Scan Mode 1. A scanned image is displayed in the main window and the small window in the display area as soon as it is scanned. 2. You may execute the following in the Scan mode. 2-1.
  • Page 71 Scanning IPs (Scan Mode) 2002. Jan. Verson 3.0 2-2. Enlarging or contracting the image display size ..The red frame of the small image corresponds to the part displayed in the image window...... To enlarge a displayed image, click the button, and click the mouse button in a position to be enlarged in the image window.
  • Page 72 Scanning IPs (Scan Mode) 2002. Jan. Verson 3.0 2-3. Changing the color of displayed images You may change the color of displayed images by clicking one of six buttons shown above. 2-4. Adjusting the gradation of displayed images If you want to change the gradation of displayed images, select a scale in the pull-down menu and adjust the gradation with the adjuster bar on the histogram or by dragging the button below the histogram.
  • Page 73 Scanning IPs (Save Mode) 2002. Jan. Verson 3.0 3. Click on the Save icon in the Scan mode window or the Save button in the lower left. The Scan mode window changes into the Save mode window, in which scanned images are saved. Save Mode You may set the following in the Save mode window.
  • Page 74 Scanning IPs (Save Mode) 2002. Jan. Verson 3.0 Automatic Suffix Rule No ........Automatic file naming is not executed. The operator should input a file name of each image file. Yes (continuative) ..Automatic file naming is executed. Suffices are added to file names.
  • Page 75 Scanning IPs (Save Mode) 2002. Jan. Verson 3.0 When Yes (continuative) and suffix type of 1, 2, 3, 4, 5 and so forth are selected and the latest scanned image is stored in the file "xxx-6", for example, the file name "xxx-7"...
  • Page 76 Scanning IPs (Save Mode) 2002. Jan. Verson 3.0 You may select one of the following two file formats. Img/Inf ......... This is the standard file format of the BAS/FLA series. Each file of this format consists of a luster file (xxx.img) and an information file (xxx.inf). Use this Img/Inf file format if you want to analyze scanned images with the ScienceLab or Array Gauge made by Fuji Photo Film Co., Ltd.
  • Page 77 Scanning IPs (Save Mode) 2002. Jan. Verson 3.0 The folder where the scanned data file is stored is displayed in the box below "Root Folder ". If you want to change the folder, click the button and change the folder. 4.
  • Page 78 2002. Jan. Verson 3.0...

  • Page 79: Part 7 Scanning Gel Samples

    2002. Jan. Verson 3.0 Part Scanning Gel Samples...
  • Page 80 1. Switch on the FLA-8000 and peripheral devices. 2. Switch on the PC. 3. Make sure that the FLA-8000 has warmed up and is ready for operation. Activate the FLA-8000 Image Reader from the Startup menu or using the shortcut key.
  • Page 81 Scanning Gel Samples (Carrier Set Mode) 2002. Jan. Verson 3.0 4. The main window of the FLA-8000 Image Reader is displayed. Carrier Set Mode 1. Select "Gel" in "Select Sample" in the Carrier Set mode. Click on the Slide icon or the radio button on the left of the Slide icon to select the gel.
  • Page 82 Note: Be careful not to make the gel buffer solution exceed the gel stopper inside the carrier. The carrier is transported at high speed in the FLA-8000 during scan- ning. If buffer solution is too much, it may spill inside, resulting in breakdown of...
  • Page 83 Scanning Gel Samples (Carrier Set Mode) 2002. Jan. Verson 3.0 2-3. Open the sample setting door. 2-4. Open the carrier locking cover.
  • Page 84 2002. Jan. Verson 3.0 2-5. Set the gel carrier. Set it so that the hinge of the gel setting cover is located on the left and the poles on the FLA-8000 are engaged with the holes in the carrier. 2-6. Close the carrier locking cover firmly. Close it so that the poles on both sides of the carrier locking cover are securely engaged with the pole holds on the FLA-8000.
  • Page 85 Scanning Gel Samples (Carrier Set Mode) 2002. Jan. Verson 3.0 2-7. Close the sample setting door. 3. Go back to the PC, and click on Setting in the PC window (on the right of Carrier Set in the upper part of the window or lower left of the window). 4.
  • Page 86 Scanning Gel Samples (Setting Mode) 2002. Jan. Verson 3.0 Setting Mode 1. The following shows the details of the scanning conditions. 1-1. Resolution ( The size of scanned gel pixels is fixed to 100 Note: Gel scanning speed The gel scanning speed is fixed to 400 mm/second. 1-2.
  • Page 87 Scanning Gel Samples (Setting Mode) 2002. Jan. Verson 3.0 Note: It is impossible to set the laser conditions independently for several pieces of gel in one-time scanning. All pieces of gel are scanned in the laser conditions dis- played before clicking the Scan button. You may set the filter and high voltage conditions independently for each piece of gel.
  • Page 88 Note: Number of gradations and dynamic range The number of gradations and dy- namic range of the FLA-8000 are fixed to the values shown below, regardless of the sample types. Number of gradations: Sixteen bits Dynamic range: Five digits...
  • Page 89 Scanning Gel Samples (Scan Mode) 2002. Jan. Verson 3.0 3. Click the Scan icon in the upper part of the Setting window or the Scan button in the lower right of the window to start final scanning. Scan Mode 1. Gel being scanned is marked with .
  • Page 90 Scanning Gel Samples (Scan Mode) 2002. Jan. Verson 3.0 Cascade ....Displays overlaid images. Tile Horizontal ... Displays images horizontally.
  • Page 91 Scanning Gel Samples (Scan Mode) 2002. Jan. Verson 3.0 Tile Vertical ....Displays images vertically. Arrange Icons ... Windows illustrated as title bars are arranged.
  • Page 92 Scanning Gel Samples (Scan Mode) 2002. Jan. Verson 3.0 3. When Catalog is selected on the Window menu, the minimized catalog window is enlarged and displayed in the forefront.
  • Page 93 Scanning Gel Samples (Scan Mode) 2002. Jan. Verson 3.0 4. Change images scanned by three types of lasers in the image window as shown below. Click on the mark in the upper part of the window to change the im- agesas shown below.
  • Page 94 Scanning Gel Samples (Scan Mode) 2002. Jan. Verson 3.0 5-2. Enlarging or contracting the image display size ..The red frame of the small image corresponds to the part displayed in the image window...... To enlarge a displayed image, click the button, and click the mouse button in a position to be enlarged in the image window.
  • Page 95 Scanning Gel Samples (Scan Mode) 2002. Jan. Verson 3.0 5-3. Changing the color of displayed images You may change the color of displayed images by clicking one of six but- tons shown above. 5-4. Adjusting the gradation of displayed images If you want to change the gradation of displayed images, select a scale in the pull-down menu and adjust the gradation with the adjuster bar on the histogram or by dragging the button below the histogram.
  • Page 96 Scanning Gel Samples (Scan Mode) 2002. Jan. Verson 3.0 6. Click on the Save icon in the Scan mode window or the Save button in the lower left. The Scan mode window changes into the Save mode window, in which scanned images are saved.
  • Page 97 Scanning Gel Samples (Save Mode) 2002. Jan. Verson 3.0 Save Mode You may set the following in the Save mode window. 1. Giving an image file name It is possible to give names to image files automatically in a certain rule. (Serial numbers are added to the end of image file names.) Each file name consists of the characters shown in the following example, in principle.
  • Page 98 Scanning Gel Samples (Save Mode) 2002. Jan. Verson 3.0 Automatic Suffix Rule No ........ Automatic file naming is not executed. The opera- should input a file name of each image file. Yes (continuative) ..Automatic file naming is executed. Suffices are added to file names.
  • Page 99 Scanning Gel Samples (Save Mode) 2002. Jan. Verson 3.0 2. Specifying an image file format Click the button or the File Format tag in the Preference dialog box of the Naming Rule Option described above. A dialog box for specifying a file format is displayed.
  • Page 100 Scanning Gel Samples (Save Mode) 2002. Jan. Verson 3.0 Img/Inf ......... This is the standard file format of the BAS/FLA series. Each file of this format consists of a luster file (xxx.img) and an information file (xxx.inf). Use this Img/Inf file format if you want to analyze scanned images with the ScienceLab or Array Gauge made by Fuji Photo Film Co., Ltd.
  • Page 101 Scanning Gel Samples (Save Mode) 2002. Jan. Verson 3.0 The box below Root Folder shows where the folder for saving the images resides. If you want to change the folder, click the button and change the folder. 4. Inputting a comment You may input a comment in the Comment box below the Name box.
  • Page 102 2002. Jan. Verson 3.0...

  • Page 103: Part 8 Setting Optional Filters

    Setting Optional Filters 2002. Jan. Verson 3.0 Part Setting Optional Filters...
  • Page 104 Setting Optional Filters 2002. Jan. Verson 3.0 Setting Optional Filters You may add another set of optional filters to the FLA-8000, in addition to three types of built-in standard filters Set the optional filters in the procedures shown below. 1. Click on the Option menu to display the pull-down menu. Select "Filter Change".
  • Page 105 Setting Optional Filters Setting Optional Filters 2002. Jan. Verson 3.0 4. Remove the filter ring from the filter block using the tool and remove the shield plate. 5. Prepare a filter of the following dimensions: Diameter: 25 mm Thickness: 4 mm or more, 12 mm or less 6.
  • Page 106 Setting Optional Filters 2002. Jan. Verson 3.0 7. Set the filter block with the filter on the front side. 8. Close the filter-adding door.

  • Page 107: Part 9 Daily Maintenance

    2002. Jan. Verson 3.0 Part Daily Maintenance...

  • Page 108: Maintenance Of Shg Laser

    The SHG laser employed in the FLA-8000 requires periodical calibration. When the FLA-8000 is switched on, it automatically executes calibration. Laser Thus, you need not carry out calibration intentionally if you use the FLA-8000 repeat- edly at a short time interval (shorter than a month). CAUTION Activate the FLA-8000 once every 30 days, at least, to execute calibration even though it is not used for a long time.

  • Page 109: Part 10 Troubleshooting

    2002. Jan. Verson 3.0 Part Troubleshooting...

  • Page 110: Troubles In Fla-8000 Body

    1 Request to close the cover A request to close the cover does not imply a trouble. The cover of the FLA-8000 body must be closed when it starts up or scans samples. If the cover is open in such a condition, the indicator lamps and beep sound indicate that the cover must be closed.
  • Page 111 Troubles in FLA-8000 body 2002. Jan. Verson 3.0 Causes and Countermeasures Cause Didn't you open the cover during the self-diagnosis and startup adjustment of the FLA-8000 (about fifteen minutes after turning on the power)? Countermeasure Close the cover. Cause Didn't you open the cover during scanning? Countermeasure Close the cover immediately.
  • Page 112 Refer to "Countermeasures according to warning and error messages" below. The indicator lamps and beep sound do not indicate occurrence of a warning. Errors An error occurs in the FLA-8000 body. Beep sound: "Be-beep, be-beep, be-beep" When an error occurs An error message dialog box is displayed on the Image Reader screen displayed on the analyzer unit.

  • Page 113: Countermeasures According To Warning And Error Messages

    <When an error occurs, remove the carrier, close the cover, and execute a self- diagnosis without switching off the FLA-8000.> * If you switch off the FLA-8000 by mistake, remove the carrier, switch on the FLA-8000, and execute a self-diagnosis.
  • Page 114 Countermeasures according to warning and error messages 2002. Jan. Verson 3.0 3. The following dialog box appears. Click the button. 4. When an error code is displayed, memorize it and click the OK button. Self-diagnosis is executed for about five minutes, and a text file having the following file name is created automatically.
  • Page 115 8. Print two text files created in steps 4 and 7. Facsimile the printout to the dealer you purchased the FLA-8000 (dealer having engineers) or Fuji Photo Film Co., Ltd. Terms Restarting the (FLA-8000) body: Turning off the body once and then turning it on again after several seconds. On-call: Calling the dealer you purchased the FLA-8000.
  • Page 116 9410H can not continue scanning. If the phenomenon does (Warning) "SHG473 stoppage error" Please restart FLA-8000 and try. not change, on-call, or The optimum temperature detection results in NG. execute self-diagnosis. The current exceeds the LD current upper limit. (Scanning with other la- A Peltier error bit is detected.
  • Page 117 No lasers are installed. special cases.) ** Analog warning in startup self-diagnosis ** Restart the FLA-8000 body. 9A01H FLA-8000 has a problem and can "5 um-400 mm/s analog adjustment error when start- If the phenomenon does (Warning) not scan with the current scanning ing up"...
  • Page 118 Countermeasures according to warning and error messages 2002. Jan. Verson 3.0 FLA-8000 has a problem and can "100-800 analog adjustment error when starting up" Restart the FLA-8000 body. 9A10H not scan with the current scanning Ditto If the phenomenon does (Warning) condition.
  • Page 119 Countermeasures according to warning and error messages 2002. Jan. Verson 3.0 FLA-8000 detected a problem. Main scanning unit error Execute self-diagnosis B031H Please see the user manual and Sensor ON error after moving out of the origin as is. run diagnostics.
  • Page 120 Countermeasures according to warning and error messages 2002. Jan. Verson 3.0 FLA-8000 detected a problem. B122H Filter unit error Execute self-diagnosis Please see the user manual and Sensor ON error after moving out of the origin as is. run diagnostics.
  • Page 121 Countermeasures according to warning and error messages 2002. Jan. Verson 3.0 While setting the CPVD focusing unit FLA-8000 detected a problem. Focusing unit error Execute self-diagnosis B454H Please see the user manual and Time-out error while moving to the origin after receiv- as is.
  • Page 122 Countermeasures according to warning and error messages 2002. Jan. Verson 3.0 FLA-8000 detected a problem. Light leak check error Execute self-diagnosis C402H Please see the user manual and Values over a specified level are counted more fre- as is. run diagnostics.
  • Page 123 Countermeasures according to warning and error messages 2002. Jan. Verson 3.0 FLA-8000 detected a problem. Moving when focusing Execute self-diagnosis A20DH Please see the user manual and as is. run diagnostics. CPLD processing time-out A30xH A processing complete signal was not received from the CPLD within the specified time.
  • Page 124 Countermeasures according to warning and error messages 2002. Jan. Verson 3.0 FLA-8000 detected a problem. A408H GET PINT COMMAND Execute self-diagnosis Please see the user manual and as is. run diagnostics. FLA-8000 detected a problem. A409H GET PRINT DATA Execute self-diagnosis Please see the user manual and as is.
  • Page 125 Countermeasures according to warning and error messages 2002. Jan. Verson 3.0 FLA-8000 detected a problem. SHG MAP COMMAND Execute self-diagnosis A510H Please see the user manual and as is. run diagnostics. FLA-8000 detected a problem. SHG MAP DATA Execute self-diagnosis...
  • Page 126 Countermeasures according to warning and error messages 2002. Jan. Verson 3.0 FLA-8000 detected a problem. MEMORY READ COMMAND Execute self-diagnosis A612H Please see the user manual and as is. run diagnostics. FLA-8000 detected a problem. MEMORY READ DATA Execute self-diagnosis...
  • Page 127 Countermeasures according to warning and error messages 2002. Jan. Verson 3.0 A715H FLA-8000 detected a problem. BATOOL Execute self-diagnosis Please see the user manual and as is. run diagnostics. A716H FLA-8000 detected a problem. CHECK Execute self-diagnosis Please see the user manual and as is.
  • Page 128 Countermeasures according to warning and error messages 2002. Jan. Verson 3.0 FLA-8000 detected a problem. CHECK Execute self-diagnosis A806H Please see the user manual and as is. run diagnostics. CPLD retry error A9xxH NG codes are received three times continuously from the CPLD in response to the command.

  • Page 129: Other Troubles

    IMPORTANT If a sample adheres to the equipment as a result of scanning an IP with the sample on it and drops onto the inner part of the FLA-8000, the inside of the FLA-8000 may possibly be RI-polluted. If inner parts of the FLA-8000 may be RI-polluted, use a radiation measuring instrument, etc.
  • Page 130 2002. Jan. Verson 3.0...

  • Page 131: Part 11 Specifications

    2002. Jan. Verson 3.0 Part Specifications...

  • Page 132: Major Specifications

    : 4 digits or more (L5 recording) Density gradations : 16 bits Using the FLA-8000 in the micro-array method is allowed to licensees only in U.S.A. Using the FLA-8000 in the micro-array method is not allowed in any countries other than U.S.A.

  • Page 133: Power Supply Facilities

    200 to 230 VAC +10% (2) Phase Single-phase (3) Frequency 50 or 60 Hz (4) Power consumption * FLA-8000 3.0 A (100 V) 1.5 A (200 V) * Analyzer unit Approx. 13 A (100 V) Approx. 7 A (200 V) * IP eraser 1.25 A (100 V)
  • Page 134 2002. Jan. Verson 3.0...

  • Page 135: Part 12 Warranty

    2002. Jan. Verson 3.0 Part Warranty...

  • Page 136: Warranty Of Fla-8000

    Warranty of FLA-8000 / Repair Service Period of Product 2002. Jan. Verson 3.0 Warranty of FLA-8000 The FLA-8000 is warranted as shown below. Warranty period: One year from the date of installation Warranty condition: Fuji Photo Film Co., Ltd. will repair, without charge, the defective product in the warranty period shown above.
  • Page 137 2002. Jan. Verson 3.0...

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