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18
Adjust the DIA field diaphragm.
Press the [F. STOP] button on the DS-L4 screen to adjust the
field diaphragm so that it almost circumscribes the field of view.
Size of the field diaphragm
Normally, the field diaphragm should be adjusted so that it
almost circumscribes the field of view. Excessively
opening the field diaphragm will result in stray light
entering the field of view, generating flare and reducing
the image contrast. It will also cause the sample to
become decolorized over a wider area.
Adjustment timing for the field diaphragm
Be sure to adjust the field diaphragm each time you
change the objective.
19
Adjusting the brightness
Press the [ND WHEEL] button on the DS-L4 screen to open the
sub screen, and then move the slider to adjust the transmittance
of the ND filter.
20
View the sample.
To observe another part of the sample, operate the ergo
controller or the joystick to move the stage. If the sample is not in
focus, turn the focus knob to focus on it.
To observe under a different magnification, repeat steps 15 and
later with another objective.
Glare when switching microscopy method
When switching from dark-field to bright-field microscopy,
you may feel the field of view is dazzling. To avoid this,
close your eyes or look away from the eyepiece during
the switchover.
Presetting the microscopy state
The MODE function can be used to record the current
state of the motorized devices for later use. For details,
refer to Chapter 5, "2 Using the MODE Function".
Chapter 4
Microscopy with DS-L4
1-35
Appropriate size of the field diaphragm
Field of view
Field diaphragm
Circumscribe around the field of view
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Table of Contents
Troubleshooting
