Page 1 Agilent 1260 Infinity Diode Array and Multiple Wavelength Detector User Manual Agilent Technologies...
Page 2 Notices Warranty © Agilent Technologies, Inc. 2006-2017, receive no greater than Restricted Rights as 2018 defined in FAR 52.227-19(c)(1-2) (June The material contained in this docu- 1987). U.S. Government users will receive No part of this manual may be reproduced ment is provided “as is,”...
Page 3 In This Guide… In This Guide… This manual covers the Agilent 1260 Infinity Diode Array and Multiple Wavelength Detector modules: • G1315C - 1260 DAD VL+ • G1365C - 1260 MWD • G1315D - 1260 DAD VL • G1365D - 1260 MWD VL 1 Introduction This chapter gives an introduction to the detector, instrument overview and internal connectors.
Page 4 In This Guide… 7 Error Information This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions. 8 Test Functions This chapter describes the detector’s built in test functions. 9 Maintenance This chapter describes the maintenance of the detector.
Page 5: Table Of Contents
Contents Contents 1 Introduction Introduction to the Detector Optical System System Overview Bio-inert Materials 2 Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications 3 Installing the Module Unpacking the Detector Optimizing the Stack Configuration Installation Information on Leak and Waste Handling Installing the Detector Flow Connections to the Detector Installing Capillaries...
Page 6 Contents 6 Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Status Indicators User Interfaces Agilent Lab Advisor Software 7 Error Information What Are Error Messages General Error Messages Detector Error Messages 8 Test Functions Self-test Filter Test Intensity Test Holmium Oxide Test ASTM Drift and Noise Test...
Page 7 Contents 10 Parts for Maintenance Overview of Maintenance Parts Standard Flow Cell Standard Flow Cell Bio-inert Semi-Micro Flow Cell Parts Micro Flow Cell Prep Flow Cell - SST Prep Flow Cell - Quartz Nano Flow Cells High Pressure Flow Cell Accessory Kits 11 Identifying Cables Cable Overview...
Page 8 The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Radio Interference Sound Emission UV-Radiation Solvent Information Declaration of Conformity for HOX2 Filter Installation of Stainless Steel Cladded PEEK Capillaries Agilent Technologies on Internet Agilent 1260 Infinity DAD and MWD User Manual...
Page 9: Introduction
Agilent 1260 Infinity DAD and MWD User Manual Introduction Introduction to the Detector Optical System System Overview Leak and Waste Handling Bio-inert Materials This chapter gives an introduction to the detector, instrument overview and internal connectors. Agilent Technologies...
Page 10: Introduction To The Detector
Introduction Introduction to the Detector Introduction to the Detector The detector is designed for highest optical performance, GLP compliance and easy maintenance. It includes the following features: • 80 Hz data acquisition rate for (ultra-) fast LC applications (requires internal hard disk, G1315C and G1365C only), •...
Page 11: Optical System
Introduction Optical System Optical System The optical system of the detector is shown in Figure below. Its illumination source is a combination of a deuterium-arc-discharge lamp for the ultraviolet (UV) wavelength range and a tungsten lamp for the visible (VIS) and short-wave near-infrared (SWNIR) wavelength range.
Page 12 Introduction Optical System Lamps The light source for the UV-wavelength range is a deuterium lamp with a shine-through aperture. As a result of plasma discharge in low-pressure deuterium gas, the lamp emits light over the 190 nm to approximately 800 nm wavelength range.
Page 13 Introduction Optical System Grating The combination of dispersion and spectral imaging is accomplished by using a concave holographic grating. The grating separates the light beam into all its component wavelengths and reflects the light onto the photodiode array. Diode Array The diode array is a series of 1024 individual photodiodes and control circuits located on a ceramic carrier.
Page 14: System Overview
Introduction System Overview System Overview Leak and Waste Handling The 1200 Infinity Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed. Figure 2 Leak and waste handling concept (overview - typical stack configuration as an example) Agilent 1260 Infinity DAD and MWD User Manual...
Page 15 Introduction System Overview The solvent cabinet (1) is designed to store a maximum volume of 6 L solvent. The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2.5 L. For details, see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets (a printed copy of the guideline has been shipped with the solvent cabinet, electronic copies are available on the Internet).
Page 16: Bio-Inert Materials
Bio-inert Materials Bio-inert Materials For the Agilent 1260 Infinity Bio-inert LC system, Agilent Technologies uses highest quality materials in the flow path (also referred to as wetted parts), which are widely accepted by life scientists, as they are known for optimum inertness to biological samples and ensure best compatibility with common samples and solvents over a wide pH range.
Page 17 Introduction Bio-inert Materials Table 1 Bio-inert materials used in Agilent 1260 Infinity Systems Module Materials Bio-inert heat-exchanger G5616-60050 PEEK (steel-cladded) (for Agilent 1290 Infinity Thermostatted Column Compartment G1316C) Bio-inert Valve heads G4235A, G5631A, G5639A: PEEK, ceramic based) Bio-inert Connection capillaries Upstream of sample introduction: •...
Page 18 Introduction Bio-inert Materials Agilent 1260 Infinity DAD and MWD User Manual...
Page 19: Site Requirements And Specifications
Agilent 1260 Infinity DAD and MWD User Manual Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications Specifications Specification Conditions This chapter provides information on environmental requirements, physical and performance specifications. Agilent Technologies...
Page 20: Site Requirements
Site Requirements and Specifications Site Requirements Site Requirements A suitable environment is important to ensure optimal performance of the instrument. Power Considerations The module power supply has wide ranging capability. It accepts any line voltage in the range described in Table 2 on page 23.
Page 21 Never operate your instrumentation from a power outlet that has no ground connection. ➔ Never use a power cord other than the Agilent Technologies power cord designed for your region. Use of unsupplied cables WA R N I N G Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury.
Page 22 Site Requirements and Specifications Site Requirements Bench Space The module dimensions and weight (see Table 2 on page 23) allow you to place the module on almost any desk or laboratory bench. It needs an additional 2.5 cm (1.0 inches) of space on either side and approximately 8 cm (3.1 inches) in the rear for air circulation and electric connections.
Page 23: Physical Specifications
Site Requirements and Specifications Physical Specifications Physical Specifications Table 2 Physical Specifications Type Specification Comments Weight 11.5 kg (26 lbs) Dimensions 140 x 345 x 435 mm (height × width × depth) (5.5 x 13.5 x 17 inches) Line voltage 100 –...
Page 24: Performance Specifications
Site Requirements and Specifications Performance Specifications Performance Specifications Specifications Performance Specifications G1315C Table 3 Performance Specifications G1315C Type Specification Comments Detection type 1024-element photodiode array Light source Deuterium and tungsten lamps The UV-lamp is equipped with RFID tag that holds lamp typical information.
Page 25 Site Requirements and Specifications Performance Specifications Table 3 Performance Specifications G1315C Type Specification Comments Flow cells Standard: 13 µL volume, 10 mm cell All flow cells are equipped with path length and 120 bar (1740 psi) RFID tags that hold cell typical pressure maximum information.
Page 26 Site Requirements and Specifications Performance Specifications Table 3 Performance Specifications G1315C Type Specification Comments Control and data Agilent ChemStation for LC (32-bit) For 1260 systems: evaluation • Revision B.04.02 DSP2 or above For 1100/1200 systems: • Revision B.01.03 or above Local Control Agilent Instant Pilot (G4208A) For 1260 systems:...
Page 27 Site Requirements and Specifications Performance Specifications Performance Specifications G1315D Table 4 Performance Specifications G1315D Type Specification Comments Detection type 1024-element photodiode array Light source Deuterium and tungsten lamps The UV-lamp is equipped with RFID tag that holds lamp typical information. Data rate up to 20 Hz Wavelength range...
Page 28 Site Requirements and Specifications Performance Specifications Table 4 Performance Specifications G1315D Type Specification Comments Flow cells Standard: 13 µL volume, 10 mm cell All flow cells are equipped with path length and 120 bar (1740 psi) RFID tags that hold cell typical pressure maximum information.
Page 29 Site Requirements and Specifications Performance Specifications Table 4 Performance Specifications G1315D Type Specification Comments Local Control Agilent Instant Pilot (G4208A) For 1260 systems: • B.02.11 or above For other systems: • B.02.09 or above Analog outputs Recorder/integrator: 100 mV or 1 V, output range 0.001 –...
Page 30 Site Requirements and Specifications Performance Specifications Performance Specifications G1365C Table 5 Performance Specifications G1365C Type Specification Comments Detection type 1024-element photodiode array Light source Deuterium and tungsten lamps The UV-lamp is equipped with RFID tag that holds lamp typical information. Data rate up to 80 Hz Wavelength range...
Page 31 Site Requirements and Specifications Performance Specifications Table 5 Performance Specifications G1365C Type Specification Comments Flow cells Standard: 13 µL volume, 10 mm cell All flow cells are equipped with path length and 120 bar (1740 psi) RFID tags that hold cell typical pressure maximum information.
Page 32 Site Requirements and Specifications Performance Specifications Table 5 Performance Specifications G1365C Type Specification Comments Control and data Agilent ChemStation for LC (32-bit) For 1260 systems: evaluation • Revision B.04.02 DSP2 or above For 1100/1200 systems: • Revision B.01.03 or above Local Control Agilent Instant Pilot (G4208A) For 1260 systems:...
Page 33 Site Requirements and Specifications Performance Specifications Performance Specifications G1365D Table 6 Performance Specifications G1365D Type Specification Comments Detection type 1024-element photodiode array Light source Deuterium and tungsten lamps The UV-lamp is equipped with RFID tag that holds lamp typical information. Data rate up to 20 Hz Wavelength range...
Page 34 Site Requirements and Specifications Performance Specifications Table 6 Performance Specifications G1365D Type Specification Comments Flow cells Standard: 13 µL volume, 10 mm cell All flow cells are equipped with path length and 120 bar (1740 psi) RFID tags that hold cell typical pressure maximum information.
Page 35 Site Requirements and Specifications Performance Specifications Table 6 Performance Specifications G1365D Type Specification Comments Control and data Agilent ChemStation for LC (32-bit) For 1260 systems: evaluation • Revision B.04.02 DSP2 or above For 1100/1200 systems: • Revision B.01.03 SR-2 / B.02.01 SR-2 or above Local Control Agilent Instant Pilot (G4208A)
Page 36: Specification Conditions
Site Requirements and Specifications Performance Specifications Specification Conditions ASTM: “Standard Practice for Variable Wavelength Photometric Detectors Used in Liquid Chromatography”. Reference conditions: cell path length 10 mm, wavelength 254 and 750 nm with reference wavelength 360 nm/100 nm, slit width 4 nm, time constant 2 s (equal to response time 4 s), flow 1 mL/min LC-grade Methanol.
Page 37: Installing The Module
Installation Information on Leak and Waste Handling Installing the Detector Flow Connections to the Detector Installing Capillaries Setting up the LAN access This chapter gives information about the preferred stack setup for your system and the installation of your module. Agilent Technologies...
Page 38: Unpacking The Detector
Unpacking the Detector Damaged Packaging If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment. "Defective on arrival" problems C AU T I O N If there are signs of damage, please do not attempt to install the module.
Page 39: Delivery Checklist
Unpacking the Detector Delivery Checklist Ensure all parts and materials have been delivered with the detector. The delivery checklist is shown below. Please report missing or damaged parts to your local Agilent Technologies sales and service office. Table 7 Detector Checklist Description...
Page 40: Optimizing The Stack Configuration
Installing the Module Optimizing the Stack Configuration Optimizing the Stack Configuration If your detector is part of a complete Agilent 1200 Series system, you can ensure optimum performance by installing the following configuration. This configuration optimizes the system flow path, ensuring minimum delay volume.
Page 41 Installing the Module Optimizing the Stack Configuration Figure 4 Recommended Stack Configuration for 1260 Infinity (Rear View) Agilent 1260 Infinity DAD and MWD User Manual...
Page 42: Two Stack Configuration
Installing the Module Optimizing the Stack Configuration Two Stack Configuration To avoid excessive height of the stack when the autosampler thermostat is added to the system it is recommended to form two stacks. Some users prefer the lower height of this arrangement even without the autosampler thermostat.
Page 43 Installing the Module Optimizing the Stack Configuration Figure 6 Recommended Two Stack Configuration for 1260 Infinity (Rear View) Agilent 1260 Infinity DAD and MWD User Manual...
Page 44: Installation Information On Leak And Waste Handling
Installing the Module Installation Information on Leak and Waste Handling Installation Information on Leak and Waste Handling The Agilent 1200 Infinity Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed.
Page 45 Installing the Module Installation Information on Leak and Waste Handling Figure 7 Leak and waste handling (overview - typical stack configuration as an example) Solvent cabinet Leak pan Leak pan's outlet port (A), leak funnel (B) and corrugated waste tube (C) Waste tube of the sampler’s needle wash Condense drain outlet of the autosampler cooler Waste tube of the purge valve...
Page 46 Installing the Module Installation Information on Leak and Waste Handling 1 Stack the modules according to the adequate stack configuration. The leak pan outlet of the upper module must be vertically positioned above the leak tray of the lower module, see Figure 7 on page 45.
Page 47 Installing the Module Installation Information on Leak and Waste Handling Figure 8 Warning label (illustration for correct waste tubing) Agilent 1260 Infinity DAD and MWD User Manual...
Page 48: Installing The Detector
Installing the Module Installing the Detector Installing the Detector Parts required Description Power cord LAN cable (cross-over or twisted pair network cable) All modules in the stack should have the latest firmware installed. If other revisions are required, check with the Agilent support for best match. Hardware required Detector (as ordered) Software required...
Page 49 Installing the Module Installing the Detector 1 Note the MAC address of the LAN interface (rear of the module, under the configuration switch, see Figure 9 on page 49). It’s required for “LAN Configuration” on page 261. 2 Place the module in the stack or on the bench in a horizontal position. 3 Ensure the line power switch at the front of the module is OFF.
Page 50 Installing the Module Installing the Detector 9 Turn on power by pushing the button at the lower left hand side of the module. The status LED should be green. Figure 10 Front View of Detector The module is turned on when the line power switch is pressed and the green indicator N O T E lamp is illuminated.
Page 51: Flow Connections To The Detector
Installing the Module Flow Connections to the Detector Flow Connections to the Detector Parts required Description G1315-68755 Accessory kit Hardware required Other modules Preparations Detector is installed in the LC system. Toxic, flammable and hazardous solvents, samples and reagents WA R N I N G The handling of solvents, samples and reagents can hold health and safety risks.
Page 52 Installing the Module Flow Connections to the Detector The heat exchanger/capillary and the cell body can be fixed mirror symmetrically to have N O T E both capillaries routed to the bottom or to the top (depending on the routing of the capillaries to the column).
Page 53 Installing the Module Flow Connections to the Detector If another Agilent module is positioned on top of the Connect the capillary from the column to the capillary detector, route the tubing assembly waste from the holder (top). Connect the PTFE waste tubing to the flow accessory kit behind the capillary holder and connect the cell outlet fitting (bottom) and the corrugated waste top end to the other module’s waste outlet.
Page 54: Installing Capillaries
Installing the Module Installing Capillaries Installing Capillaries In May 2013, Agilent has introduced new UHP-FF fittings, which are designed for improved robustness and ease of use. Previous fittings require careful handling. Therefore it is important to know, which fittings are used in the system.
Page 55 Installing the Module Installing Capillaries Installing UHP-FF Fittings Tools required Description 5043-0915 Fitting mounting tool for bio-inert capillaries Parts required Description Capillaries and For details refer to the part section of the manual. Fittings 1 Slide the fitting on the capillary. Let the capillary jut out 5 mm. Agilent 1260 Infinity DAD and MWD User Manual...
Page 56 Installing the Module Installing Capillaries 2 Insert the fitting to the receiving port and push the capillary to the bottom of the port. 3 Finger tighten the nut into the port until snug. Agilent 1260 Infinity DAD and MWD User Manual...
Page 57 Installing the Module Installing Capillaries 4 Use Fitting mounting tool (5043-0915) or a 5 mm hex wrench for fixing the fitting (maximum torque 0.8 Nm). Potential damage of capillaries C AU T I O N ➔ Do not remove fittings from used capillaries. Agilent 1260 Infinity DAD and MWD User Manual...
Page 58 Installing the Module Installing Capillaries 5 When using UHP-FF fittings with bioinert capillaries, do not try to remove fittings from these capillaries. Bio-inert capillaries are using a PEEK front end, which may expand under pressure especially when being in contact with some organic solvents.
Page 59 Installing the Module Installing Capillaries Installation of the Bio-inert Zero Dead Volume (ZDV) Union The Bio-inert ZDV (p/n 5067-4741) union has two different connectors where capillaries need to be installed in the correct sequence. Otherwise, an inset of the union may be damaged and the connection may not be tight. Potential leak or damage of the Bio-inert ZDV Union.
Page 60: Setting Up The Lan Access
Installing the Module Setting up the LAN access Setting up the LAN access Please follow the instructions in “LAN Configuration” on page 261 Agilent 1260 Infinity DAD and MWD User Manual...
Page 61: Using The Detector
Two detectors of same type (e.g. G1315C/D and G1315C/D) Two detectors of similar type (e.g. G1315C/D and G1315A/B) Solvent Information This chapter provides information on how to set up the detector for an analysis and explains the basic settings. Agilent Technologies...
Page 62: Leak And Waste Handling
Using the Detector Leak and Waste Handling Leak and Waste Handling Toxic, flammable and hazardous solvents, samples and reagents WA R N I N G The handling of solvents, samples and reagents can hold health and safety risks. ➔ When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.
Page 63: Setting Up An Analysis
Using the Detector Setting up an Analysis Setting up an Analysis This chapter may be used to • Prepare the system, • Get to know the set up of an HPLC analysis and • Use it as an instrument check to demonstrate that all modules of the system are correctly installed and connected.
Page 64 Using the Detector Setting up an Analysis Table 8 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation Isopropanol Best solvent to flush air out of the system When switching between reverse Isopropanol Best solvent to flush air out of the phase and normal phase (both times) system After an installation...
Page 65: Requirements And Conditions
Using the Detector Setting up an Analysis Requirements and Conditions Parts and Material required Table 9 on page 65 lists the parts and material you need for the set up of the analysis. Some of these are optional (not required for the basic system). Table 9 Parts and Material required Agilent 1260...
Page 66 Using the Detector Setting up an Analysis Typical Chromatogram A typical chromatogram for this analysis is shown in Figure 14 on page 66. The exact profile of the chromatogram will depend on the chromatographic conditions. Variations in solvent quality, column packing, standard concentration and column temperature will all have a potential effect on peak retention and response.
Page 67: Optimization Of The System
Using the Detector Setting up an Analysis Optimization of the System The settings used for this analysis are specific for this purpose. For other applications the system can be optimized in various ways. Please refer to the section “Optimizing the Detector” on page 91.
Page 68: Preparing The Hplc System
Using the Detector Setting up an Analysis Preparing the HPLC System 1 Turn on the Agilent ChemStation PC and the monitor. 2 Turn on the HPLC modules. 3 Start the Agilent ChemStation software. If the pump, autosampler, thermostatted column compartment and detector are found, the ChemStation screen should look like shown in Figure below.
Page 69 Using the Detector Setting up an Analysis 4 Turn on the detector lamp, pump and autosampler by clicking the System On button or the buttons below the module icons on the graphical user interface (GUI). After some time, the pump, thermostatted column compartment and detector module will turn to green.
Page 70 Using the Detector Setting up an Analysis 5 Purge the pump. For more information see “Priming and Purging the System” on page 63. 6 Allow the detector to warm up at least 60 minutes to provide a stable baseline (see example in Figure 17 on page 70 and Table 11...
Page 71 Using the Detector Setting up an Analysis 7 For the isocratic pump, fill the solvent bottle with the mixture of HPLC-grade bi-distilled water (30 %) and acetonitrile (70 %). For binary- and quaternary pumps you can use separate bottles. 8 Click on the Load Method button and select DEF_LC.M and press OK. Alternative double-click on the method in the method window.
Page 72 Using the Detector Setting up an Analysis 9 Click on the module icons (see Figure below) and open the Setup of these modules. Figure on page 73 shows the detector settings (do not change the detector parameters at this time). Figure 19 Open the module menu Agilent 1260 Infinity DAD and MWD User Manual...
Page 73 Using the Detector Setting up an Analysis 10 Enter the pump parameters mentioned under Table 10 on page 65. • up to 8 signals (A to H) with individual wavelength settings can be selected. • spectrum settings, “Spectrum Settings (DAD only)”...
Page 74 Using the Detector Setting up an Analysis 12 Click the button and select Change... to open the Signal Plot information. Select the Pump: Pressure and the DAD A: Signal 254,4 as signals. Change the Y-range for the DAD to 1 mAU and the offset to 20% and the pressure offset to 50%.
Page 75 Using the Detector Setting up an Analysis The Online Plot (Figure 21 on page 75) shows both, the pump pressure and the detector absorbance signals. Pressing Adjust the signals can be reset to the offset value and Balance would do a balance on the detector. Figure 21 Online Plot Window 13 If both baselines are stable, set the Y-range for the detector signal to...
Page 76 Using the Detector Setting up an Analysis 14 Select the menu item RunControl -> Sample Info and enter information about this application (see figure below). Press OK to leave this screen. Figure 22 Sample Information 15 Fill the content of an isocratic standard sample ampoule into a vial and seal the vial with a cap and place the vial into autosampler tray (position #1).
Page 77: Running The Sample And Verifying The Results
Using the Detector Setting up an Analysis Running the Sample and Verifying the Results 1 To start a run select the menu item RunControl -> Run Method. 2 This will start the modules and the online plot on the Agilent ChemStation will show the resulting chromatogram.
Page 78: Special Settings Of The Detector
Using the Detector Special Settings of the Detector Special Settings of the Detector In this chapter special settings of the G1315C/D and G1365C/D are described (based on the Agilent ChemStation B.02.01). Control Settings • Lamps: turn on and off of UV- and Vis lamp •...
Page 79: Configuration Settings
Using the Detector Special Settings of the Detector Configuration Settings • Temperature Control: the optical unit is kept on constant temperature and improves the baseline stability in unstable environments. See also note below. • UV lamp tag: for Agilent lamps with I.D. tags. If no I.D. tag lamp is used, detector icon will become grey (lamp tag not ready) and analysis is disabled.
Page 80: Online Spectra (Dad Only)
Using the Detector Special Settings of the Detector Online Spectra (DAD only) 1 To view the online spectra during the run select Online Spectra. Figure 26 Online Spectra Window 2 Change the absorbance and wavelength range according your needs. Agilent 1260 Infinity DAD and MWD User Manual...
Page 81: Run Recovery Settings
Using the Detector Special Settings of the Detector Run Recovery Settings For this recovery mode the CompactFlash Card must be in the detector. C AU T I O N If the LAN communiaction is interruppted, no data is stored. ➔ Have the CompactFlash Card always inserted.
Page 82: Automated Run Recovery In Case Of Temporary Communication Failures
Using the Detector Special Settings of the Detector Automated Run Recovery in case of temporary communication failures Table 12 Automated Run Recovery in case of temporary communication failures Situation Reaction On ChemStation All OK • Run in progress - Data Analysis •...
Page 83 Using the Detector Special Settings of the Detector Table 12 Automated Run Recovery in case of temporary communication failures Situation Reaction On ChemStation Stop time • Run in progress - Data Analysis elapsed • Prerun / Rawdata • Elapsed run time stops •...
Page 84: Manual Run Recovery In Case Of Permanent Communication Failures
Using the Detector Special Settings of the Detector Manual Run Recovery in case of permanent communication failures see Note below Start a recover After a recover When during recovery an error “Method/Sequence stopped” appears, the instrument N O T E logbook shows an entry “No Run data available in device“.
Page 85: Analog Output Settings
Using the Detector Special Settings of the Detector Analog Output Settings To change the Output Range of the analog outputs see “Control Settings” page 78. 1 To change the offset and the attenuation select Analog Outputs. 2 Change the ranges for absorbance and wavelength according your needs. Zero Offset Limits: 1 to 99 % in steps of 1 % Attenuation Limits: 0.98 to 2000 mAU at discrete values for either 100 mV or 1 V full scale...
Page 86: Spectrum Settings (Dad Only)
Using the Detector Special Settings of the Detector Spectrum Settings (DAD only) To change the Spectra settings open. 1 To change the Spectra settings select Setup Detector Signals. 2 In the section Spectrum click on the drop-down list and chose a parameter. Table 13 on page 87 shows the possible parameters.
Page 87 Using the Detector Special Settings of the Detector Table 13 Spectrum Settings Store Defines at which points on “signal A” spectra will be taken and saved. Signal A is used to control the “peak controlled spectra acquisition”; the other signals have no influence on spectra acquisition. None No spectra are taken.
Page 88: Peakwidth Settings
Using the Detector Special Settings of the Detector Peakwidth Settings Do not use peak width shorter than necessary N O T E Do not use 0.025 sec response time (no filtering/high noise and no need (actually ultra-fast LC doesn't deliver peaks < 0.0025 min / < 0.15 sec) 1 To change the Peakwidth settings select Setup Detector Signals.
Page 89 Using the Detector Special Settings of the Detector Table 14 Peak Width — Response Time — Data Rate Peak Width (min) Response Time Data Rate (Hz) Detector (sec) <0.0025 0.025 G1315C/G1365C only >0.0025 0.05 G1315C/G1365C only >0.005 G1315C/G1365C only >0.01 G1315C/D and G1365C/D >0.03...
Page 90: Slit Settings
Using the Detector Special Settings of the Detector Slit Settings 1 To change the Slit settings select Setup Detector Signals. 2 In the section Slit click on the drop-down list. 3 Change the Slit width according to your needs. The Slit group allows you to select the optical bandwidth of the detector;...
Page 91: Margin For Negative Absorbance Settings
Using the Detector Special Settings of the Detector Margin for Negative Absorbance Settings 1 To change the settings select Setup Detector Signals. 2 In the section Margin for Negative Absorbance change the value according to your needs. Use this field to modify the detector’s signal handling to increase the margin for negative absorbance.
Page 92: Special Setups With Multiple Dad-Mwds
Using the Detector Special Setups with Multiple DAD-MWDs Special Setups with Multiple DAD-MWDs The G1315C/D and G1365C/D detectors are based on the same new hardware/electronic N O T E platform. The G1315A/B and G1365A/B detectors are based on the old hardware/electronic platform.
Page 93: Solvent Information
Using the Detector Solvent Information Solvent Information Observe the following recommendations on the use of solvents. • Follow recommendations for avoiding the growth of algae, see pump manuals. • Small particles can permanently block capillaries and valves. Therefore, always filter solvents through 0.4 μm filters. •...
Page 94 Using the Detector Solvent Information PEEK PEEK (Polyether-Ether Ketones) combines excellent properties regarding biocompatibility, chemical resistance, mechanical and thermal stability. PEEK is therefore the material of choice for UHPLC and biochemical instrumentation. It is stable in the specified pH range (for the Bio-inert LC system: pH 1 – 13, see bio-inert module manuals for details), and inert to many common solvents.
Page 95 Using the Detector Solvent Information Tantalum (Ta) Tantalum is inert to most common HPLC solvents and almost all acids except fluoric acid and acids with free sulfur trioxide. It can be corroded by strong bases (e.g. hydroxide solutions > 10 %, diethylamine). It is not recommended for the use with fluoric acid and fluorides.
Page 96 Using the Detector Solvent Information Diamond-Like Carbon (DLC) Diamond-Like Carbon is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. Fused silica and Quartz (SiO Fused silica is used in 1290 Infinity Flow Cells and capillaries. Quartz is used for classical flow cell windows.
Page 97 Using the Detector Solvent Information Fluorinated polymers (PTFE, PFA, FEP, FFKM) Fluorinated polymers like PTFE (polytetrafluorethylene), PFA (perfluoroalkoxy) and FEP (fluorinated ethylene propylene) are inert to almost all common acids, bases, and solvents. FFKM is perfluorinated rubber, which is also resistant to most chemicals. As an elastomer, it may swell in some organic solvents like halogenated hydrocarbons.
Page 98 Using the Detector Solvent Information Agilent 1260 Infinity DAD and MWD User Manual...
Page 99: How To Optimize The Detector
Sample and Reference Wavelength and Bandwidth Slit Width Optimizing Spectral Acquisition (DAD only) Margin for Negative Absorbance Optimizing Selectivity Quantifying Coeluting Peaks by Peak Suppression Ratio Qualifiers for Selective Detection of Compound Classes This chapter provides information on how to optimize the detector. Agilent Technologies...
Page 100: Introduction
How to optimize the Detector Introduction Introduction The detector has a variety of parameters that can be used to optimize performance. Depending on whether signal or spectral data need to be optimized, different settings are recommended. The following sections describe optimization for: •...
Page 101: Optimization Overview
How to optimize the Detector Optimization Overview Optimization Overview Table 15 Optimization Overview Parameter Impact 1 Selection of flow cell • peak resolution versus sensitivity • Choose flow cell according to used column, see Figure 32 on page 102. 2 Connection of flow cell •...
Page 102 How to optimize the Detector Optimization Overview Table 15 Optimization Overview Parameter Impact 5 Setting the slit width • Use 4 nm slit for normal applications. • spectral resolution, sensitivity and • Use narrow slit (e.g 1 nm) if your analytes have narrow absorbance bands linearity.
Page 103: Optimizing For Sensitivity, Selectivity, Linearity And Dispersion
How to optimize the Detector Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Flow Cell Path Length Lambert-Beer’s law shows a linear relationship between the flow cell path length and absorbance. where T is the transmission, defined as the quotient of the intensity of the transmitted light I divided by the intensity of the incident light, I ε...
Page 104 How to optimize the Detector Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Figure 33 Influence of Cell Path Length on Signal Height Traditionally LC analysis with UV detectors is based on comparing measurements with internal or external standards. To check photometric accuracy of the detector it is necessary to have more precise information on path lengths of the flow cells.
Page 105: Peak Width (Response Time)
How to optimize the Detector Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Peak width (response time) Response time describes how fast the detector signal follows a sudden change of absorbance in the flow cell. The detector uses digital filters to adapt response time to the width of the peaks in your chromatogram.
Page 106 How to optimize the Detector Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Table 17 Peak Width — Response Time — Data Rate Peak Width Response Time Data Rate [Hz] Detector [minutes] [seconds] <0.0025 0.025 G1315C/G1365C only >0.0025 0.05 G1315C/G1365C only >0.005 G1315C/G1365C only >0.01...
Page 107: Sample And Reference Wavelength And Bandwidth
How to optimize the Detector Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Sample and Reference Wavelength and Bandwidth The detector measures absorbance simultaneously at wavelengths from 190 to 950 nm. Two lamps provide good sensitivity over the whole wavelength range. The deuterium discharge lamp provides the energy for the UV range (190 to 400 nm) and the tungsten lamp emits light from 400 to 950 nm for the visible and short wave near infrared.
Page 108 How to optimize the Detector Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Figure 35 Optimization of Wavelength Setting A wide bandwidth has the advantage of reducing noise by averaging over a wavelength range — compared to a 4 nm bandwidth, the baseline noise is reduced by a factor of approximately 2.5, whereas the signal is about 75 % of a 4 nm wide band.
Page 109 How to optimize the Detector Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Because the detector averages absorbance values that are calculated for each wavelength, using a wide bandwidth does not negatively impact linearity. The use of a reference wavelength is highly recommended to further reduce baseline drift and wander induced by room temperature fluctuations or refractive index changes during a gradient.
Page 110: Slit Width
How to optimize the Detector Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Slit Width The detector has a variable slit at the entrance of the spectrograph. This is an effective tool to adapt the detector to changing demand of different analytical problems.
Page 111 How to optimize the Detector Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Figure 39 Influence of the Slit Width on Baseline Noise However, with a wider slit, the spectrograph’s optical resolution (its ability to distinguish between different wavelengths) diminishes. Any photodiode receives light within a range of wavelength determined by the slit width.
Page 112: Optimizing Spectral Acquisition (Dad Only)
How to optimize the Detector Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Optimizing Spectral Acquisition (DAD only) Storage of all spectra consumes a lot of disk space. It is very useful to have all spectra available during optimization of a method or when analyzing unique samples.
Page 113: Optimizing Selectivity
How to optimize the Detector Optimizing Selectivity Optimizing Selectivity Quantifying Coeluting Peaks by Peak Suppression In chromatography, two compounds may often elute together. A conventional dual-signal detector can only detect and quantify both compounds independently from each other if their spectra do not overlap. However, in most cases this is highly unlikely.
Page 114 How to optimize the Detector Optimizing Selectivity With a UV-visible detector based on a diode array and the correct choice of a reference wavelength setting, quantitative detection is possible. To suppress caffeine, the reference wavelength must be set to 282 nm. At this wavelength, caffeine shows exactly the same absorbance as at 222 nm.
Page 115: Ratio Qualifiers For Selective Detection Of Compound Classes
How to optimize the Detector Optimizing Selectivity Ratio Qualifiers for Selective Detection of Compound Classes Ratio qualifiers can be used where, in a complex sample, only one particular class needs to be analyzed — a parent drug and its metabolites in a biological sample, for example.
Page 116 How to optimize the Detector Optimizing Selectivity Figure 43 Selectivity by Ratio Qualifiers In a four-component mixture, only biphenyl was recorded. The other three peaks were suppressed because they did not meet the ratio-qualifier criterion and therefore the output was set to zero. The characteristic wavelengths 249 nm (λ...
Page 117: Troubleshooting And Diagnostics
Overview of the Module’s Indicators and Test Functions Status Indicators Power Supply Indicator Module Status Indicator User Interfaces Agilent Lab Advisor Software This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces. Agilent Technologies...
Page 118: Overview Of The Module's Indicators And Test Functions
Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Overview of the Module’s Indicators and Test Functions Status Indicators The module is provided with two status indicators which indicate the operational state (prerun, run, and error states) of the module. The status indicators provide a quick visual check of the operation of the module.
Page 119: Status Indicators
Troubleshooting and Diagnostics Status Indicators Status Indicators Two status indicators are located on the front of the module. The lower left indicates the power supply status, the upper right indicates the module status. Figure 44 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch.
Page 120: Module Status Indicator
Troubleshooting and Diagnostics Status Indicators Module Status Indicator The module status indicator indicates one of six possible module conditions: • When the status indicator is OFF (and power switch light is on), the module is in a prerun condition, and is ready to begin an analysis. •...
Page 121: User Interfaces
Troubleshooting and Diagnostics User Interfaces User Interfaces Depending on the used interface, the available tests and the screens/reports may vary. N O T E Preferred tool should be the Agilent Diagnostic Software, see “Agilent Lab Advisor Software” on page 122. Screenshots used within these procedures are based on the Agilent ChemStation.
Page 122: Agilent Lab Advisor Software
Troubleshooting and Diagnostics Agilent Lab Advisor Software Agilent Lab Advisor Software The Agilent Lab Advisor software is a standalone product that can be used with or without data system. Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet.
Page 123: Error Information
UV Lamp Current UV Lamp Voltage UV Ignition Failed UV Heater Current Calibration Values Invalid Holmium Oxide Test Failed Illegal Temperature Value from Sensor on Main Board Illegal Temperature Value from Sensor at Air Inlet Wavelength Recalibration Lost Agilent Technologies...
Page 124 Error Information Agilent Lab Advisor Software Heater at fan assembly failed Heater Power At Limit DSP Not Running No Run Data Available In Device Instrument Logbook This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions.
Page 125: What Are Error Messages
Error Information What Are Error Messages What Are Error Messages Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs which requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary).
Page 126: General Error Messages
Error Information General Error Messages General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well. Timeout Error ID: 0062 The timeout threshold was exceeded. Probable cause Suggested actions Check the logbook for the occurrence and The analysis was completed successfully, source of a not-ready condition.
Page 127: Shutdown
Error Information General Error Messages Shutdown Error ID: 0063 An external instrument has generated a shutdown signal on the remote line. The module continually monitors the remote input connectors for status signals. A LOW signal input on pin 4 of the remote connector generates the error message.
Page 128: Lost Can Partner
Error Information General Error Messages Lost CAN Partner Error ID: 0071 During an analysis, the internal synchronization or communication between one or more of the modules in the system has failed. The system processors continually monitor the system configuration. If one or more of the modules is no longer recognized as being connected to the system, the error message is generated.
Page 129: Leak Sensor Open
Error Information General Error Messages Leak Sensor Open Error ID: 0083 The leak sensor in the module has failed (open circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak-sensor current to change within defined limits.
Page 130: Compensation Sensor Short
Error Information General Error Messages Compensation Sensor Short Error ID: 0080 The ambient-compensation sensor (NTC) on the main board in the module has failed (open circuit). The resistance across the temperature compensation sensor (NTC) on the main board is dependent on ambient temperature. The change in resistance is used by the leak circuit to compensate for ambient temperature changes.
Page 131: Leak
Error Information General Error Messages Leak Error ID: 0064 A leak was detected in the module. The signals from the two temperature sensors (leak sensor and board-mounted temperature-compensation sensor) are used by the leak algorithm to determine whether a leak is present. When a leak occurs, the leak sensor is cooled by the solvent.
Page 132: Additional Information
Error Information General Error Messages Additional Information A leak error may be caused by the Aligent 1200 sampler. In each sampler injection sequence, step# 2 ejects the mobile phase stored in the metering head during the previous injection. This mobile phase is ejected through the short plastic tube connected to port# 4 of the sampler switching valve.
Page 133: Open Cover
Error Information General Error Messages 2 If it is desired that the system has only one leak drain tube, then it's possible in increase the length of the small plastic tube which is connected to port# 4 of the sampler switching valve. This tube can then be taken to waste separately.
Page 134: Detector Error Messages
Error Information Detector Error Messages Detector Error Messages These errors are detector specific. Visible Lamp Current The visible lamp current is missing. The processor continually monitors the lamp current during operation. If the current falls below the lower current limit, the error message is generated. Probable cause Suggested actions Ensure the visible lamp connector is seated firmly.
Page 135: Diode Current Leakage
Error Information Detector Error Messages Diode Current Leakage Error ID: 1041 When the detector is switched on, the processor checks the leakage current of each of the optical diodes. If the leakage current exceeds the upper limit, the error message is generated. Probable cause Suggested actions Please contact your Agilent service...
Page 136: Uv Lamp Voltage
Error Information Detector Error Messages UV Lamp Voltage Error ID: 7451 The UV lamp anode voltage is missing. The processor continually monitors the anode voltage across the lamp during operation. If the anode voltage falls below the lower limit, the error message is generated.
Page 137: Uv Heater Current
Error Information Detector Error Messages UV Heater Current Error ID: 7453 The UV lamp heater current is missing. During UV lamp ignition, the processor monitors the heater current. If the current does not rise above the lower limit within one second, the error message is generated.
Page 138: Holmium Oxide Test Failed
Error Information Detector Error Messages Holmium Oxide Test Failed Probable cause Suggested actions Ensure the lamps are switched on. Lamps switched off. Ensure the flow cell is inserted correctly, and is Defective or dirty flow cell. free from contamination (cell windows, buffers etc.).
Page 139: Illegal Temperature Value From Sensor At Air Inlet
Error Information Detector Error Messages Illegal Temperature Value from Sensor at Air Inlet Error ID: 1072 This temperature sensor delivered a value outside the allowed range. The parameter of this event equals the measured temperature in 1/100 centigrade. As a result the temperature control is switched off. Probable cause Suggested actions •...
Page 140: Heater At Fan Assembly Failed
Error Information Detector Error Messages Heater at fan assembly failed Error ID: 1073 Every time the deuterium lamp or the tungsten lamp (DAD only) is switched on or off a heater self-test is performed. If the test fails an error event is created.
Page 141: Dsp Not Running
Error Information Detector Error Messages DSP Not Running This error message comes up when the communication between the optical unit and the main board has a problem. Probable cause Suggested actions • Switch the detector off and on again at the Random communication error.
Page 142: Instrument Logbook
Error Information Detector Error Messages Instrument Logbook Figure 45 Instrument Logbook The logbook does not indicate a communication loss (power fail). It just shows the N O T E recovering (Power on, Lamps on). Agilent 1260 Infinity DAD and MWD User Manual...
Page 143: Test Functions
Holmium Oxide Test Test Evaluation ASTM Drift and Noise Test Test Evaluation Cell Test Test Evaluation Using the Built-in Test Chromatogram Procedure Using the Agilent Lab Advisor Wavelength Verification and Calibration This chapter describes the detector’s built in test functions. Agilent Technologies...
Page 144: Self-Test
Test Functions Self-test Self-test The DAD self-test (see Figure 46 on page 144) runs a series of individual tests, and evaluates the results automatically. The following tests are run: • Filter Test • Slit Test • Dark Current Test • Intensity Test •...
Page 145: Filter Test
Test Functions Filter Test Filter Test The filter test checks the correct operation of the filter assembly. When the test is started, the holmium oxide filter is moved into position. During filter movement, the absorbance signal is monitored. As the edge of the filter passes through the light path, an absorbance maximum is seen.
Page 146 Test Functions Filter Test Figure 47 Filter Test Figure 48 Filter Test (Signals) Agilent 1260 Infinity DAD and MWD User Manual...
Page 147: Test Evaluation
Test Functions Filter Test Test Evaluation Filter Test Failed Test Failed Probable cause Suggested actions Install the filter assembly. Filter assembly (lever and filter) not installed. Please contact your Agilent service Defective filter motor. representative. Holmium Oxide Maximum out of Limits Probable cause Suggested actions Install the holmium oxide filter.
Page 148: Intensity Test
Test Functions Intensity Test Intensity Test The test is for the standard flow cells (10 mm and 6 mm pathlength) only. The nano-flow N O T E cells (80 nL and 500 nL) cannot be run with this test due to its low volume. The intensity test measures the intensity of the deuterium and tungsten lamps over the full wavelength range (190 –...
Page 149 Test Functions Intensity Test Figure 49 Intensity Test Figure 50 Intensity Test (Signal) In case of low counts in one or more ranges, start the testing with the comparison of values with flow cell vs. flow cell removed. Contaminations of the cell windows and/or the lenses (there are 3 between vis-lamp and flow cell), will reduce the light throughput.
Page 150 Test Functions Intensity Test If the detector fails in the range 501 nm - 950 nm, check • is the VIS-lamp ON? If not, turn it on. • is VIS-lamp glass bulb blackended or broken? If yes, replace VIS-lamp. • does the UV-lamp show a reflective coating towards the VIS-lamp? If yes, replace UV-lamp.
Page 151: Holmium Oxide Test
Test Functions Holmium Oxide Test Holmium Oxide Test The holmium oxide test uses characteristic absorbance maxima of the built-in holmium oxide filter to verify wavelength accuracy (see also “Wavelength Verification and Calibration” on page 160). When the test is started, the 1-nm slit is moved into the light path automatically.
Page 152 Test Functions Holmium Oxide Test Figure 51 Holmium Oxide Test Figure 52 Holmium Oxide Test (Signal) Agilent 1260 Infinity DAD and MWD User Manual...
Page 153 Test Functions Holmium Oxide Test Test Failed Probable cause Suggested actions Ensure the flow cell is filled with water. Absorbing solvent or air bubble in flow cell. Recalibrate (see “Wavelength Verification and Incorrect calibration Calibration” on page 160) and repeat the test. Run the cell test (see “Cell Test”...
Page 154: Astm Drift And Noise Test
Test Functions ASTM Drift and Noise Test ASTM Drift and Noise Test The ASTM noise test determines the detector noise over a period of 20 minutes. The test is done with the flowcell removed, so the test results are not influenced by solvent or pump effects.
Page 155 Test Functions ASTM Drift and Noise Test Test Failed Probable cause Suggested actions Allow lamp to warm-up for at least 1 hour. Insufficient lamp warm-up time Exchange the lamp. Old or non-Agilent lamp. Agilent 1260 Infinity DAD and MWD User Manual...
Page 156: Cell Test
Test Functions Cell Test Cell Test The cell test measures the intensity of the deuterium and tungsten lamps over the full wavelength range (190 – 950 nm), once with the flow cell installed, and once with the flow cell removed. The resulting intensity ratio is a measure of the amount of light absorbed by the flow cell.
Page 157 Test Functions Cell Test Figure 54 Cell Test Figure 55 Cell Test (Signals) This test can be used for the standard flow cells only. The nano flow cells will give very N O T E low values due to their design. Test Failed (low ratio value) Probable cause Suggested actions...
Page 158: Using The Built-In Test Chromatogram
Test Functions Using the Built-in Test Chromatogram Using the Built-in Test Chromatogram This function is available from the Agilent ChemStation, Lab Advisor and Instant Pilot. The built-in Test Chromatogram can be used to check the signal path from the detector to the data system and the data analysis or via the analog output to the integrator or data system.
Page 159 Test Functions Using the Built-in Test Chromatogram 6 To start a test chromatogram enter in the command line: STRT Figure 56 Test Chromatogram with Agilent Lab Advisor 7 To stop the test chromatogram enter in the command line: STOP The test chromatogram is switched off automatically at the end of a run. N O T E Agilent 1260 Infinity DAD and MWD User Manual...
Page 160: Wavelength Verification And Calibration
Test Functions Wavelength Verification and Calibration Wavelength Verification and Calibration The detector uses the alpha (656.1 nm) and beta (486 nm) emission lines of the deuterium lamp for wavelength calibration. The sharp emission lines enable more accurate calibration than is possible with holmium oxide. When verification is started, the 1-nm slit is moved into the light path automatically, and the gain is set to zero.
Page 161 Test Functions Wavelength Verification and Calibration Figure 58 Wavelength Verification Figure 59 Wavelength Calibration Wavelength calibration should be done • after maintenance of the flow cell, • lamp exchange, or • after a major repair, like processor board or optical unit exchange, see also “Replacing the Module’s Firmware”...
Page 162 Test Functions Wavelength Verification and Calibration Agilent 1260 Infinity DAD and MWD User Manual...
Page 163: Maintenance
Nano Flow Cell - Replacing or Cleaning Cleaning or Exchanging the Holmium Oxide Filter Correcting Leaks Replacing Leak Handling System Parts Replacing the CompactFlash Card (G1315C/G1365C only) Replacing the Module’s Firmware This chapter describes the maintenance of the detector. Agilent Technologies...
Page 164: Introduction To Maintenance
Maintenance Introduction to Maintenance Introduction to Maintenance The module is designed for easy maintenance. Maintenance can be done from the front with module in place in the system stack. There are no serviceable parts inside. N O T E Do not open the module. Agilent 1260 Infinity DAD and MWD User Manual...
Page 165: Cautions And Warnings
Maintenance Cautions and Warnings Cautions and Warnings Toxic, flammable and hazardous solvents, samples and reagents WA R N I N G The handling of solvents, samples and reagents can hold health and safety risks. ➔ When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.
Page 166 Maintenance Cautions and Warnings Personal injury or damage to the product WA R N I N G Agilent is not responsible for any damages caused, in whole or in part, by improper use of the products, unauthorized alterations, adjustments or modifications to the products, failure to comply with procedures in Agilent product user guides, or use of the products in violation of applicable laws, rules or regulations.
Page 167: Overview Of Maintenance
Maintenance Overview of Maintenance Overview of Maintenance The following pages describe maintenance (simple repairs) of the detector that can be carried out without opening the main cover. Table 19 Overview of Maintenance Procedure Typical Frequency Notes Cleaning of module If required. Deuterium lamp or If noise and/or drift exceeds your application limits or An intensity test should be performed...
Page 168: Cleaning The Module
Maintenance Cleaning the Module Cleaning the Module The module case should be kept clean. Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent. Do not use an excessively damp cloth allowing liquid to drip into the module. Liquid dripping into the electronic compartment of your module can cause shock WA R N I N G hazard and damage the module...
Page 169: Exchanging A Lamp
Maintenance Exchanging a Lamp Exchanging a Lamp When If noise or drift exceeds application limits or lamp does not ignite Tools required Description Screwdriver, Pozidriv #1 PT3 Parts required Description 2140-0820 Longlife Deuterium lamp “C“ (with black cover and RFID tag) G1103-60001 Tungsten lamp Preparations...
Page 170 Maintenance Exchanging a Lamp Press the release buttons and remove the front cover to Disconnect lamp from the connector, unscrew the gain access to the flow cell area. Vis-lamp (left) and/or UV-lamp (right) and remove the lamp. Do not touch the glass bulb with your fingers. When replacing the Vis-lamp, assure that the Vis-lamp is Insert the lamp.
Page 171 Maintenance Exchanging a Lamp Next Steps: Replace the front cover. Reset the lamp counter as described in the user interface documentation (lamps with I.D. tag cannot be reset). Turn the lamp on and give the lamp 10 minutes to warm Perform a “Wavelength Verification and Calibration”...
Page 172: Exchanging A Flow Cell
Maintenance Exchanging a Flow Cell Exchanging a Flow Cell For bio-inert modules use bio-inert parts only! When If an application needs a different type of flow cell or the flow cell needs repair. Tools required Description Wrench, 1/4 inch for capillary connections 5043-0915 Fitting mounting tool for bio-inert capillaries...
Page 173 Maintenance Exchanging a Flow Cell Press the release buttons and remove the front cover to Open the flow cell cover. gain access to the flow cell area. N O T E Depending on the system setup, the inlet capillary might be routed directly from the module above or below to the cell and not to the capillary holder.
Page 174 Maintenance Exchanging a Flow Cell Remove the flow cell while pressing the flow cell holder. Insert the flow cell while pressing the flow cell holder. N O T E The label attached to the flow cell provides information on part number, path length, volume and maximum pressure.
Page 175 Maintenance Exchanging a Flow Cell Next Steps: Insert the flow cell capillaries into the union holder (top is inlet, bottom is outlet). Tighten the thumb screw and Reconnect the waste tubing (bottom) to the union. Perform a “Wavelength Verification and Calibration” page 160 or a “Holmium Oxide Test”...
Page 176: Maintenance Of Standard, Semi-Micro Or Micro Flow Cell
Maintenance Maintenance of Standard, Semi-Micro or Micro Flow Cell Maintenance of Standard, Semi-Micro or Micro Flow Cell For bio-inert modules use bio-inert parts only! When If the flow cell needs repair due to leaks or contaminations (reduced light throughput) Tools required Description Wrench, 1/4 inch for capillary connections...
Page 177 Maintenance Maintenance of Standard, Semi-Micro or Micro Flow Cell Use a 4 mm hex key to unscrew the window assembly Use a tooth pick to remove the quartz window from the [1] and remove the gasket [2] from the cell body. window assembly.
Page 178 Maintenance Maintenance of Standard, Semi-Micro or Micro Flow Cell Orientation of Flow Cell Parts (“Standard Flow Cell” Assemble the washers and the window assembly in page 208) correct order. N O T E Gaskets # 6 and #7 have different hole diameters. Correct orientation of spring washers [2] is required.
Page 179 Maintenance Maintenance of Standard, Semi-Micro or Micro Flow Cell Press the window assembly onto the new or cleaned Insert the window assembly [1] into the cell body. quartz window. Next Steps: Using a 4-mm hex key, tighten the window screw hand tight plus a quarter turn. Reconnect the capillaries, see “Exchanging a Flow Cell”...
Page 180: Maintenance Of High Pressure Flow Cell
Maintenance Maintenance of High Pressure Flow Cell Maintenance of High Pressure Flow Cell When If the flow cell needs repair due to leaks or contaminations (reduced light throughput) Tools required Description 1/4 inch wrench for capillary connections hexagonal key 4 mm Tooth picks Parts required Description...
Page 181 Maintenance Maintenance of High Pressure Flow Cell Use a 4 mm hex key to unscrew the window assembly Use a tooth pick to remove the quartz window from the [1] and remove the gasket [2] from the cell body. window assembly. N O T E If the washers fall out of the window assembly, they must be inserted in the correct order with the...
Page 182: Replacing Capillaries On A Standard Flow Cell
Maintenance Replacing Capillaries on a Standard Flow Cell Replacing Capillaries on a Standard Flow Cell For bio-inert modules use bio-inert parts only! When If the capillary is blocked Tools required Description Wrench, 1/4 inch for capillary connections 5043-0915 Fitting mounting tool for bio-inert capillaries Wrench, 4 mm (for capillary connections)
Page 183 Maintenance Replacing Capillaries on a Standard Flow Cell Sample degradation and contamination of the instrument C AU T I O N Metal parts in the flow path can interact with the bio-molecules in the sample leading to sample degradation and contamination. ➔...
Page 184 Maintenance Replacing Capillaries on a Standard Flow Cell To replace the inlet capillary, use a 4-mm wrench for the Unscrew the cell body from the heat exchanger and the fitting. heat exchanger from the clamp unit. Use a small flat screw driver to carefully lift off the I.D. Unscrew the fixing screw and unwrap the inlet capillary tag.
Page 185 Maintenance Replacing Capillaries on a Standard Flow Cell Take the new inlet capillary and bend it 90° about 35 mm Bend the capillary again by 90° as shown below. from its end. Insert the capillary into the hole between fixing screw The capillary lays in the grove and should be tied around and the inlet fitting.
Page 186 Maintenance Replacing Capillaries on a Standard Flow Cell Insert the fixing screw, so that the capillary cannot leave Carefully insert the I.D. tag into the new heat exchanger. the grove. Shown is the default orientation. See Note at the beginning of this section. Fix the heat exchanger to the clamp unit and the flow Fix the inlet capillary to the flow cell body handtight first.
Page 187 Maintenance Replacing Capillaries on a Standard Flow Cell Next Steps: Check for a centered holder vs. hole. If required adjust with the holder screws. Reconnect the capillaries, see “Exchanging a Flow Cell” on page 172. Perform a leak test. Insert the flow cell. Replace the front cover.
Page 188: Replacing Capillaries On A Semi-Micro And Micro Flow Cell
Maintenance Replacing Capillaries on a Semi-Micro and Micro Flow Cell Replacing Capillaries on a Semi-Micro and Micro Flow Cell When If the capillary is blocked Tools required Description Wrench, 1/4 inch for capillary connections Wrench, 4 mm (for capillary connections) Screwdriver, Pozidriv #1 PT3 Parts required Description...
Page 189 Maintenance Replacing Capillaries on a Semi-Micro and Micro Flow Cell Identify the inlet and outlet capillaries. After replacing the outlet capillary, fix it handtight first. Then do a 1/4 turn with a 4-mm wrench. To replace the inlet capillary, use a 4-mm wrench for the Unscrew the cell body from the heat exchanger and the fitting.
Page 190 Maintenance Replacing Capillaries on a Semi-Micro and Micro Flow Cell Use a small flat screw driver to carefully lift off the I.D. Carefully insert the I.D. tag into the new heat exchanger. tag. Shown is the default orientation. See Note at the Shown is the default orientation.
Page 191 Maintenance Replacing Capillaries on a Semi-Micro and Micro Flow Cell Next Steps: Check for a centered holder vs. hole. If required adjust with the holder screws. Reconnect the capillaries, see “Exchanging a Flow Cell” on page 172. Perform a leak test. Insert the flow cell.
Page 192: Nano Flow Cell - Replacing Or Cleaning
Maintenance Nano Flow Cell - Replacing or Cleaning Nano Flow Cell - Replacing or Cleaning When If parts are contaminated or leaky. Tools required Description Screwdriver, Pozidriv #1 PT3 Wrench, 1/4 inch for capillary connections Parts required Description For parts identification refer to “Nano Flow Cells”...
Page 193 Maintenance Nano Flow Cell - Replacing or Cleaning Disconnect the capillaries from the capillary holder and Unscrew the cell body from the holder. remove the flow cell. Unscrew the capillaries from the flow cell. DO NOT use Using for example a toothpick, press on the plastic part the adapter at this time! and slide the quartz body out of the cell housing.
Page 194 Maintenance Nano Flow Cell - Replacing or Cleaning The quartz body and the cell seal assembly can be This figure shows the correct holding of the quartz body separated for cleaning purpose. and the cell seal assembly. Replace the cell seal assembly onto the quartz body. Slide the quartz body completely into the cell body to the Always use a new seal assembly to exclude damage front stop (use for example a toothpick).
Page 195 Maintenance Nano Flow Cell - Replacing or Cleaning Insert the flow cell capillaries and tighten them Reassemble the flow cell body to the holder. fingertight. Use the wrench and torque adapter as described on Figure 60 on page 196 and tighten the fittings alternately.
Page 196 Maintenance Nano Flow Cell - Replacing or Cleaning With the instrument accessory kit comes a 4-mm wrench and with the Sealing Kit a N O T E special adapter. Both together work as a torque wrench with pre-defined torque (maximum allowed torque for the cell fittings is 0.7 Nm).
Page 197: Cleaning Or Exchanging The Holmium Oxide Filter
Maintenance Cleaning or Exchanging the Holmium Oxide Filter Cleaning or Exchanging the Holmium Oxide Filter When If holmium oxide filter is contaminated Tools required Description Screwdriver, Pozidriv #1 PT3 Screwdriver, flat blade Wrench, 1/4 inch for capillary connections Pair of tweezers Parts required Description 79880-22711...
Page 198 Maintenance Cleaning or Exchanging the Holmium Oxide Filter Unscrew the six screws and remove the flow cell cover. If not already in this position, move the filter up. While releasing the holder with a screw driver (at the While releasing the holder with a screw driver, carefully top), carefully remove the holmium oxide filter.
Page 199 Maintenance Cleaning or Exchanging the Holmium Oxide Filter Next Steps: Replace the flow cell cover and fix the six screws. Perform a holmium oxide test, see “Holmium Oxide Test” on page 151 to check the proper function of the holmium oxide filter. Insert the flow cell, see “Exchanging a Flow Cell”...
Page 200: Correcting Leaks
Maintenance Correcting Leaks Correcting Leaks When If a leakage has occurred in the flow cell area or at the heat exchanger or at the capillary connections Tools required Description Tissue Wrench, 1/4 inch for capillary connections 5043-0915 Fitting mounting tool for bio-inert capillaries Preparations Remove the front cover.
Page 201: Replacing Leak Handling System Parts
Maintenance Replacing Leak Handling System Parts Replacing Leak Handling System Parts When If the parts are corroded or broken Tools required None Parts required Description 5041-8388 Leak funnel 5041-8389 Leak funnel holder 5062-2463 Corrugated tubing, PP, 6.5 mm id, 5 m Preparations Remove the front cover.
Page 202: Replacing The Compactflash Card (G1315C/G1365C Only)
Maintenance Replacing the CompactFlash Card (G1315C/G1365C only) Replacing the CompactFlash Card (G1315C/G1365C only) When If defective Tools required None Parts required Description 01100-68700 CompactFlash Card Kit Preparations Turn the detector OFF and have access to the rear of the detector. The G1315C and G1365C is equipped with a CompactFlash card.
Page 203: Replacing The Module's Firmware
Maintenance Replacing the Module’s Firmware Replacing the Module’s Firmware When The installation of newer firmware might be necessary • if a newer version solves problems of older versions or • to keep all systems on the same (validated) revision. The installation of older firmware might be necessary •...
Page 204 Maintenance Replacing the Module’s Firmware Module Specific Information Table 20 Module Specific Information (G1315C/D and G1365C/D) G1315C DAD VL+ / G1365C G1315D DAD / G1365D MWD Initial firmware (main B.01.02 B.01.04 and resident) Compatibility with When using the G1315C/D and G1365C/D in a system, all other 1260/1290 Infinity modules must have firmware revision A.06.xx or B.06.xx or above (main modules...
Page 205: Parts For Maintenance
Standard Flow Cell Bio-inert Semi-Micro Flow Cell Parts Micro Flow Cell Prep Flow Cell - SST Prep Flow Cell - Quartz Nano Flow Cells High Pressure Flow Cell Accessory Kits This chapter provides information on parts for maintenance. Agilent Technologies...
Page 206: Overview Of Maintenance Parts
Parts for Maintenance Overview of Maintenance Parts Overview of Maintenance Parts Item Description 5065-9982 Plastics kit (includes base, top, left and right sides) G4208-67001 Instant Pilot G4208A (requires firmware B.02.08 or above) Flow cells with ID tag G1315-87311 Capillary ST 0.17 mm x 380 mm S/S 5022-6515 Union ZDV G1315-68707...
Page 207 Parts for Maintenance Overview of Maintenance Parts Figure 64 Maintenance Parts Agilent 1260 Infinity DAD and MWD User Manual...
Page 208: Standard Flow Cell
Parts for Maintenance Standard Flow Cell Standard Flow Cell Item Description G1315-60022 Standard flow cell, 10 mm, 13 µL, 120 bar (12 MPa) 79883-22402 Window screw 5062-8553 Washer kit (10/pk) 79883-28801 Compression washer 79883-22301 Window holder 1000-0488 Quartz window G1315-68711 Gasket BACK (PTFE), 2.3 mm hole, outlet side (12/pk) G1315-68710 Gasket FRONT (PTFE), 1.3 mm hole, inlet side (12/pk)
Page 209 Parts for Maintenance Standard Flow Cell Figure 65 Standard Flow Cell Parts Gaskets # 6 and #7 have different hole diameters. N O T E Figure 66 Orientation of Spring Washers Agilent 1260 Infinity DAD and MWD User Manual...
Page 210: Standard Flow Cell Bio-Inert
Parts for Maintenance Standard Flow Cell Bio-inert Standard Flow Cell Bio-inert Item Description G5615-60022 Standard flow cell bio-inert, 10 mm, 13 µL, 120 bar (12 MPa) for MWD/DAD, includes Capillary Kit Flow Cells BIO (p/n G5615-68755) G5615-68755 Capillary Kit Flow Cells BIO includes Capillary PK 0.18 mm x 1.5 m and PEEK Fittings 10/PK (p/n 5063-6591) 79883-22402 Window screw...
Page 211 Parts for Maintenance Standard Flow Cell Bio-inert Figure 67 Standard Flow Cell Bio-inert Figure 68 Orientation of Spring Washers Agilent 1260 Infinity DAD and MWD User Manual...
Page 212: Semi-Micro Flow Cell Parts
Parts for Maintenance Semi-Micro Flow Cell Parts Semi-Micro Flow Cell Parts Item Description G1315-60025 Semi-micro flow cell, 6 mm, 5 µL, 120 bar (12 MPa) 79883-22402 Window screw 5062-8553 Washer kit (10/pk) 79883-28801 Compression washer 79883-22301 Window holder 1000-0488 Quartz window 79883-68702 Gasket BACK (PTFE), 1.8 mm hole, outlet side (12/pk) G1315-68710...
Page 213 Parts for Maintenance Semi-Micro Flow Cell Parts Figure 69 Semi-Micro Flow Cell Parts Gaskets # 6 and #7 have different hole diameters. N O T E Figure 70 Orientation of Spring Washers Agilent 1260 Infinity DAD and MWD User Manual...
Page 214: Micro Flow Cell
Parts for Maintenance Micro Flow Cell Micro Flow Cell Item Description G1315-60024 Micro flow cell, 3 mm, 2 µL, 120 bar (12 MPa) 79883-22402 Window screw 5062-8553 Washer kit (10/pk) 79883-28801 Compression washer 79883-22301 Window holder 1000-0488 Quartz window 79883-68702 Gasket BACK (PTFE), 1.8 mm hole, outlet side (12/pk) G1315-68710 Gasket FRONT (PTFE), 1.3 mm hole, inlet side (12/pk)
Page 215 Parts for Maintenance Micro Flow Cell Figure 71 Micro Flow Cell Parts Gaskets # 6 and #7 have different hole diameters. N O T E Figure 72 Orientation of Spring Washers Agilent 1260 Infinity DAD and MWD User Manual...
Page 216: Prep Flow Cell - Sst
Parts for Maintenance Prep Flow Cell - SST Prep Flow Cell - SST For more details on the Preparative Flow Cells refer to the technical note that comes with N O T E the flow cells. Item Description G1315-60016 Prep flow cell SST - 3 mm, 120 bar (12 MPa) 79883-22402 Window screw 5062-8553...
Page 217 Parts for Maintenance Prep Flow Cell - SST Figure 73 Prep Flow Cell - SST Parts Gaskets # 6 and #7 have different hole diameters. N O T E Figure 74 Orientation of Spring Washers Agilent 1260 Infinity DAD and MWD User Manual...
Page 218: Prep Flow Cell - Quartz
Parts for Maintenance Prep Flow Cell - Quartz Prep Flow Cell - Quartz For more details on the Preparative Flow Cells refer to the technical note that comes with N O T E the flow cells. Item Description G1315-60017 Prep flow cell quartz, 0.3 mm, 20 bar (2 MPa) G1315-60018 Prep flow cell quartz, 0.06 mm (2 MPa) G1315-67301...
Page 219 Parts for Maintenance Prep Flow Cell - Quartz Figure 75 Prep Flow Cell - Quartz Parts Agilent 1260 Infinity DAD and MWD User Manual...
Page 220: Nano Flow Cells
Parts for Maintenance Nano Flow Cells Nano Flow Cells The following kits are available: Table 21 Nano-flow cell kits Part number Comments Semi-nano flow cell kit, 10 mm, 500 nL, 5 completely assembled (includes items 1, 2, 3, 4, 10, MPa (G1315-68724) 11, 12, 13, 14, 15, and 16) Nano flow cell kit, 6 mm, 80 nL, 5 MPa (...
Page 221 Parts for Maintenance Nano Flow Cells Figure 76 Content of kits Agilent 1260 Infinity DAD and MWD User Manual...
Page 222 Parts for Maintenance Nano Flow Cells Specific parts for the semi-nano flow cell Item Description G1315-68724 Semi-nano flow cell kit, 10 mm, 500 nL, 5 MPa G1315-87333 PEEK coated fused silica capillary Inlet (100 µm) pre-mounted to cell, includes Inlet capillary, 300 mm long, 100 µm i.d. with pre-fixed ferrules (#4) and fittings (#3), plus one PEEK Fitting FT (#5) G1315-87338 PEEK coated fused silica capillary Outlet (100 µm) pre-mounted to...
Page 223 Parts for Maintenance Nano Flow Cells Specific parts for the nano flow cell Item Description G1315-68716 Nano flow cell kit, 6 mm, 80 nL, 5 MPa G1315-87323 PEEK coated fused silica capillary Inlet (50 µm) alternative, includes Inlet capillary, 400 mm long, 50 µm i.d. with pre-fixed ferrules (#4) and fittings (#3), plus one PEEK Fitting FT (#5) G1315-87328 PEEK coated fused silica capillary Outlet (50 µm), alternative,...
Page 224: High Pressure Flow Cell
Parts for Maintenance High Pressure Flow Cell High Pressure Flow Cell Item Description G1315-60015 High pressure flow cell, 6 mm, 1.7 µL, 400 bar (40 MPa) Window assembly, comprises items 2, 3, 4, 5 and 6 79883-27101 Seal ring 1000-0953 Quartz window 79883-28802 Compression washer...
Page 225 Parts for Maintenance High Pressure Flow Cell Figure 77 High pressure flow cell - parts Agilent 1260 Infinity DAD and MWD User Manual...
Page 226: Accessory Kits
Parts for Maintenance Accessory Kits Accessory Kits Accessory kit (G1315-68755) contains some accessories and tools needed for installation and repair of the module. Item Description 5063-6527 Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.2 m (to waste) 5062-2462 Tube PTFE 0.8 mm x 2 m, re-order 5 m 0100-1516 Fitting male PEEK, 2/pk G1315-87311...
Page 227 Parts for Maintenance Accessory Kits Figure 78 Waste Tubing Parts Figure 79 Inlet Capillary (Column-Detector) Parts Agilent 1260 Infinity DAD and MWD User Manual...
Page 228 Parts for Maintenance Accessory Kits Agilent 1260 Infinity DAD and MWD User Manual...
Page 229: Identifying Cables
Agilent 1260 Infinity DAD and MWD User Manual Identifying Cables Cable Overview Analog Cables Remote Cables BCD Cables CAN/LAN Cables Agilent 1200 module to PC This chapter provides information on cables used with the Agilent 1200 Infinity Series modules. Agilent Technologies...
Page 230: Cable Overview
Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent module to 3394/6 integrators...
Page 231 Identifying Cables Cable Overview CAN cables Description 5181-1516 CAN cable, Agilent module to module, 0.5 m 5181-1519 CAN cable, Agilent module to module, 1 m LAN cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection)
Page 232: Analog Cables
Identifying Cables Analog Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules. The other end depends on the instrument to which connection is being made. Agilent Module to 3394/6 Integrators p/n 35900-60750 Pin 3394/6 Pin Agilent Signal Name...
Page 233 Identifying Cables Analog Cables Agilent Module to BNC Connector p/n 8120-1840 Pin BNC Pin Agilent Signal Name module Shield Shield Analog - Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog +...
Page 234: Remote Cables
Identifying Cables Remote Cables Remote Cables One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Agilent Module to 3396A Integrators...
Page 235 Identifying Cables Remote Cables Agilent Module to 3396 Series III / 3395B Integrators p/n 03396-61010 Pin 33XX Pin Agilent Signal Name Active module (TTL) 1 - White Digital ground 2 - Brown Prepare run 3 - Gray Start 4 - Blue Shut down 5 - Pink connected...
Page 236 Identifying Cables Remote Cables Agilent Module to General Purpose p/n 01046-60201 Wire Color Pin Agilent Signal Name Active module (TTL) White Digital ground Brown Prepare run Gray Start Blue Shut down Pink connected Yellow Power on High Ready High Green Stop Black Start request...
Page 237: Bcd Cables
Identifying Cables BCD Cables BCD Cables One end of these cables provides a 15-pin BCD connector to be connected to the Agilent modules. The other end depends on the instrument to be connected to Agilent Module to General Purpose p/n G1351-81600 Wire Color Pin Agilent Signal Name...
Page 238 Identifying Cables BCD Cables Agilent Module to 3396 Integrators p/n 03396-60560 Pin 3396 Pin Agilent Signal Name BCD Digit module BCD 5 BCD 7 BCD 6 BCD 4 BCD0 BCD 3 BCD 2 BCD 1 Digital ground + 5 V Agilent 1260 Infinity DAD and MWD User Manual...
Page 239: Can/Lan Cables
Identifying Cables CAN/LAN Cables CAN/LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors. CAN Cables Description 5181-1516 CAN cable, Agilent module to module, 0.5 m 5181-1519 CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203...
Page 240: Agilent 1200 Module To Pc
Identifying Cables Agilent 1200 module to PC Agilent 1200 module to PC Description G1530-60600 RS-232 cable, 2 m RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It's also called "Null Modem Cable"...
Page 241: Hardware Information
Electrical Connections Serial Number Information (ALL) Rear view of the module Interfaces Interfaces Overview Setting the 8-bit Configuration Switch Special Settings Instrument Layout Early Maintenance Feedback (EMF) This chapter describes the detector in more detail on hardware and electronics. Agilent Technologies...
Page 242: Firmware Description
Hardware Information Firmware Description Firmware Description The firmware of the instrument consists of two independent sections: • a non-instrument specific section, called resident system • an instrument specific section, called main system Resident System This resident section of the firmware is identical for all Agilent 1100/1200/1220/1260/1290 series modules.
Page 243 Hardware Information Firmware Description Firmware Updates Firmware updates can be done using your user interface: • PC and Firmware Update Tool with local files on the hard disk • Instant Pilot (G4208A) with files from a USB Flash Disk • Agilent Lab Advisor software B.01.03 and above The file naming conventions are: PPPP_RVVV_XXX.dlb, where PPPP is the product number, for example, 1315AB for the G1315A/B DAD,...
Page 244 Hardware Information Firmware Description Some modules are limited in downgrading due to their main board version or their initial N O T E firmware revision. For example, a G1315C DAD SL cannot be downgraded below firmware revision B.01.02 or to a A.xx.xx. Some modules can be re-branded (e.g.
Page 245: Electrical Connections
There are no externally accessible fuses, because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations.
Page 246: Serial Number Information (All)
Hardware Information Electrical Connections Serial Number Information (ALL) The serial number information on the instrument labels provide the following information: CCXZZ00000 Format Country of manufacturing • DE = Germany • JP = Japan • CN = China Alphabetic character A-Z (used by manufacturing) Alpha-numeric code 0-9, A-Z, where each combination unambiguously denotes a module (there can be more than one code for the same module)
Page 247: Interfaces
Hardware Information Interfaces Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces: Table 22 Agilent 1200 Infinity Series Interfaces Module LAN/BCD RS-232 Analog Special (optional) (on-board) Remote Pumps G1310B Iso Pump G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump K1312B Bin Pump Clinical Ed.
Page 248 Hardware Information Interfaces Table 22 Agilent 1200 Infinity Series Interfaces Module LAN/BCD RS-232 Analog Special (optional) (on-board) Remote Detectors G1314B VWD VL G1314C VWD VL+ G1314E/F VWD K1314F Clinical Ed. G4212A/B DAD K4212B DAD Clinical Ed. G1315C DAD VL+ G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD...
Page 249 Hardware Information Interfaces Table 22 Agilent 1200 Infinity Series Interfaces Module LAN/BCD RS-232 Analog Special (optional) (on-board) Remote G4227A Flex Cube CAN-DC- OUT for CAN slaves G4240A CHIP CUBE CAN-DC- OUT for CAN slaves THERMOSTAT for G1330A/B (NOT USED), K1330B Requires a HOST module with on-board LAN (e.g.
Page 250: Interfaces Overview
Hardware Information Interfaces Interfaces Overview The CAN is inter-module communication interface. It is a 2-wire serial bus system supporting high speed data communication and real-time requirement. The modules have either an interface slot for an LAN card (e.g. Agilent G1369B/C LAN Interface) or they have an on-board LAN interface (e.g. detectors G1315C/D DAD and G1365C/D MWD).
Page 251 Hardware Information Interfaces Table 23 RS-232C Connection Table Direction Function Ground Figure 82 RS-232 Cable Analog Signal Output The analog signal output can be distributed to a recording device. For details refer to the description of the module’s main board. Agilent 1260 Infinity DAD and MWD User Manual...
Page 252 APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
Page 253 Hardware Information Interfaces Table 24 Remote Signal Distribution Signal Description DGND Digital ground PREPARE (L) Request to prepare for analysis (for example, calibration, detector lamp on). Receiver is any module performing pre-analysis activities. START (L) Request to start run / timetable. Receiver is any module performing run-time controlled activities.
Page 254: Setting The 8-Bit Configuration Switch
Hardware Information Setting the 8-bit Configuration Switch Setting the 8-bit Configuration Switch The 8-bit configuration switch is located at the rear of the module. Switch settings provide configuration parameters for LAN, serial communication protocol and instrument specific initialization procedures. All modules with on-board LAN: •...
Page 255 Hardware Information Setting the 8-bit Configuration Switch To perform any LAN configuration, SW1 and SW2 must be set to OFF. For details on the N O T E LAN settings/configuration refer to chapter LAN Configuration. Table 25 8-bit Configuration Switch (with on-board LAN) Mode Function SW 1...
Page 256: Special Settings
Hardware Information Setting the 8-bit Configuration Switch For explanation of "Boot Resident System" and "Revert to Default Data (Coldstart)" refer N O T E “Special Settings” on page 256. Special Settings The special settings are required for specific actions (normally in a service case).
Page 257 Hardware Information Setting the 8-bit Configuration Switch Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings. Loss of data C AU T I O N Forced cold start erases all methods and data stored in the non-volatile memory. Exceptions are calibration settings, diagnosis and repair log books which will not be erased.
Page 258: Instrument Layout
Hardware Information Instrument Layout Instrument Layout The industrial design of the module incorporates several innovative features. It uses Agilent’s E-PAC concept for the packaging of electronics and mechanical assemblies. This concept is based upon the use of expanded polypropylene (EPP) layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed.
Page 259: Early Maintenance Feedback (Emf)
Hardware Information Early Maintenance Feedback (EMF) Early Maintenance Feedback (EMF) Maintenance requires the exchange of components which are subject to wear or stress. Ideally, the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions, and not on a predefined time interval.
Page 260 Hardware Information Early Maintenance Feedback (EMF) Agilent 1260 Infinity DAD and MWD User Manual...
Page 261: Lan Configuration
Changing the IP Address of an Instrument Using the Agilent BootP Service Storing the settings permanently with Bootp Manual Configuration With Telnet With the Instant Pilot (G4208A) This chapter provides information on connecting the detector to the Agilent ChemStation PC. Agilent Technologies...
Page 262: What You Have To Do First
LAN Configuration What you have to do first What you have to do first The module has an on-board LAN communication interface. 1 Note the MAC (Media Access Control) address for further reference. The MAC or hardware address of the LAN interfaces is a world wide unique identifier.
Page 263: Tcp/Ip Parameter Configuration
LAN Configuration TCP/IP parameter configuration TCP/IP parameter configuration To operate properly in a network environment, the LAN interface must be configured with valid TCP/IP network parameters. These parameters are: • IP address • Subnet Mask • Default Gateway The TCP/IP parameters can be configured by the following methods: •...
Page 264: Configuration Switch
LAN Configuration Configuration Switch Configuration Switch The configuration switch can be accessed at the rear of the module. Figure 86 Location of Configuration Switch The module is shipped with all switches set to OFF, as shown above. To perform any LAN configuration, SW1 and SW2 must be set to OFF. N O T E Table 28 Factory Default Settings...
Page 265: Initialization Mode Selection
LAN Configuration Initialization mode selection Initialization mode selection The following initialization (init) modes are selectable: Table 29 Initialization Mode Switches SW 6 SW 7 SW 8 Init Mode Bootp Bootp & Store Using Stored Using Default DHCP Requires firmware B.06.40 or above. Modules without LAN on board, see G1369C LAN Interface Card Bootp When the initialization mode Bootp is selected, the module tries to download the parameters from a Bootp Server.
Page 266 LAN Configuration Initialization mode selection Bootp & Store When Bootp & Store is selected, the parameters obtained from a Bootp Server become the active parameters immediately. In addition, they are stored to the non-volatile memory of the module. Thus, after a power cycle they are still available.
Page 267 LAN Configuration Initialization mode selection Using Stored When initialization mode Using Stored is selected, the parameters are taken from the non-volatile memory of the module. The TCP/IP connection will be established using these parameters. The parameters were configured previously by one of the described methods. Figure 89 Using Stored (Principle) Using Default...
Page 268 LAN Configuration Initialization mode selection Since the default IP address is a so-called local address, it will not be routed by any network device. Thus, the PC and the module must reside in the same subnet. The user may open a Telnet session using the default IP address and change the parameters stored in the non-volatile memory of the module.
Page 269: Dynamic Host Configuration Protocol (Dhcp)
LAN Configuration Dynamic Host Configuration Protocol (DHCP) Dynamic Host Configuration Protocol (DHCP) General Information (DHCP) The Dynamic Host Configuration Protocol (DHCP) is an auto configuration protocol used on IP networks. The DHCP functionality is available on all Agilent HPLC modules with on-board LAN Interface or LAN Interface Card, and “B”-firmware (B.06.40 or above).
Page 270: Setup (Dhcp)
LAN Configuration Dynamic Host Configuration Protocol (DHCP) Setup (DHCP) Software required The modules in the stack must have at least firmware from set A.06.34 and the above mentioned modules B.06.40 or above (must from the same firmware set). 1 Note the MAC address of the LAN interface (provided with G1369C LAN Interface Card or Main Board).
Page 271 LAN Configuration Dynamic Host Configuration Protocol (DHCP) Table 32 LC Modules inclusive 1120/1220 (configuration switch at rear of the instru- ment) SW 6 SW 7 SW 8 Initialization Mode DHCP 3 Turn on the module that hosts the LAN interface. 4 Configure your Control Software (e.g.
Page 272: Link Configuration Selection
LAN Configuration Link configuration selection Link configuration selection The LAN interface supports 10 or 100 Mbps operation in full- or half-duplex modes. In most cases, full-duplex is supported when the connecting network device - such as a network switch or hub - supports IEEE 802.3u auto-negotiation specifications.
Page 273: Automatic Configuration With Bootp
LAN Configuration Automatic Configuration with BootP Automatic Configuration with BootP All examples shown in this chapter will not work in your environment. You need your own N O T E IP-, Subnet-Mask- and Gateway addresses. Assure that the detector configuration switch is set properly. The setting should be either N O T E BootP or BootP &...
Page 274: How Bootp Service Works
LAN Configuration Automatic Configuration with BootP How BootP Service Works When an instrument is powered on, an LAN Interface in the instrument broadcasts a request for an IP address or host name and provides its hardware MAC address as an identifier. The Agilent BootP Service answers this request and passes a previously defined IP address and host name associated with the hardware MAC address to the requesting instrument.
Page 275: Installation Of Bootp Service
LAN Configuration Automatic Configuration with BootP Installation of BootP Service Before installing and configuring the Agilent BootP Service, be sure to have the IP addresses of the computer and instruments on hand. 1 Log on as Administrator or other user with Administrator privileges. 2 Close all Windows programs.
Page 276 LAN Configuration Automatic Configuration with BootP 11 Files load; when finished, the BootP Settings screen appears. Figure 93 BootP Settings screen 12 In the Default Settings part of the screen, if known, you can enter the subnet mask and gateway. Defaults can be used: •...
Page 277: Two Methods To Determine The Mac Address
LAN Configuration Automatic Configuration with BootP Two Methods to Determine the MAC Address Enabling logging to discover the MAC address using BootP If you want to see the MAC address, select the Do you want to log BootP requests? check box. 1 Open BootP Settings from Start >...
Page 278: Assigning Ip Addresses Using The Agilent Bootp Service
LAN Configuration Automatic Configuration with BootP Assigning IP Addresses Using the Agilent BootP Service The Agilent BootP Service assigns the Hardware MAC address of the instrument to an IP address. Determining the MAC address of the instrument using BootP Service 1 Power cycle the Instrument.
Page 279 LAN Configuration Automatic Configuration with BootP The Do you want to log BootP requests? box must be unchecked when you have finished configuring instruments; otherwise, the log file will quickly fill up disk space. 3 Click Edit BootP Addresses... The Edit BootP Addresses screen appears. 4 Click Add...
Page 280 LAN Configuration Automatic Configuration with BootP 5 Make these entries for the instrument: • MAC address • Host name, Enter a Hostname of your choice. The Host Name must begin with "alpha" characters (i.e. LC1260) • IP address • Comment (optional) •...
Page 281: Changing The Ip Address Of An Instrument Using The Agilent Bootp Service
LAN Configuration Automatic Configuration with BootP Changing the IP Address of an Instrument Using the Agilent BootP Service Agilent BootP Service starts automatically when your PC reboots. To change Agilent BootP Service settings, you must stop the service, make the changes, and then restart the service.
Page 282 LAN Configuration Automatic Configuration with BootP Editing the IP address and other parameters in EditBootPSettings 1 Select Start > All Programs > Agilent BootP Service and select Edit BootP Settings. The BootP Settings screen appears. 2 When the BootP Settings screen is first opened, it shows the default settings from installation.
Page 283: Storing The Settings Permanently With Bootp
LAN Configuration Storing the settings permanently with Bootp Storing the settings permanently with Bootp If you want to change parameters of the module using the Bootp follow the instructions below. 1 Turn off the module. 2 Change the module's settings of the Configuration Switch to “Bootp & Store”...
Page 284: Manual Configuration
LAN Configuration Manual Configuration Manual Configuration Manual configuration only alters the set of parameters stored in the non-volatile memory of the module. It never affects the currently active parameters. Therefore, manual configuration can be done at any time. A power cycle is mandatory to make the stored parameters become the active parameters, given that the initialization mode selection switches are allowing Figure 98 Manual Configuration (Principle)
Page 285: With Telnet
LAN Configuration Manual Configuration With Telnet Whenever a TCP/IP connection to the module is possible (TCP/IP parameters set by any method), the parameters may be altered by opening a Telnet session. 1 Open the system (DOS) prompt window by clicking on Windows START button and select “Run...”.
Page 286 LAN Configuration Manual Configuration 3 Type ? and press enter to see the available commands. Figure 101 Telnet Commands Table 34 Telnet Commands Value Description displays syntax and descriptions of commands displays current LAN settings ip <x.x.x.x> sets new ip address sm <x.x.x.x>...
Page 287 LAN Configuration Manual Configuration 5 Use the “/” and press Enter to list the current settings. information about the LAN interface MAC address, initialization mode Initialization mode is Using Stored active TCP/IP settings TCP/IP status - here ready connected to PC with controller software (e.g. Agilent ChemStation), here not connected Figure 102 Telnet - Current settings in "Using Stored"...
Page 288 LAN Configuration Manual Configuration 7 When you have finished typing the configuration parameters, type exit and press Enter to exit with storing parameters. Figure 104 Closing the Telnet Session If the Initialization Mode Switch is changed now to “Using Stored” mode, the instrument N O T E will take the stored settings when the module is re-booted.
Page 289: With The Instant Pilot (G4208A)
LAN Configuration Manual Configuration With the Instant Pilot (G4208A) To configure the TCP/IP parameters before connecting the module to the network, the Instant Pilot (G4208A) can be used. 1 From the Welcome screen press the More button. 2 Select Configure. 3 Press the DAD button.
Page 290 LAN Configuration Manual Configuration Agilent 1260 Infinity DAD and MWD User Manual...
Page 291 Declaration of Conformity for HOX2 Filter Installation of Stainless Steel Cladded PEEK Capillaries First Step: Finger-tight Fitting Second Step: Installation to Connector Removing Capillaries Agilent Technologies on Internet This chapter provides addition information on safety, legal and web. Agilent Technologies...
Page 292: Appendix Safety Information
Appendix Safety Information Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument.
Page 293 Appendix Safety Information the instrument must be made inoperative and be secured against any intended operation. Make sure that only fuses with the required rated current and of the specified type (normal blow, time delay, and so on) are used for replacement. The use of repaired fuses and the short-circuiting of fuse holders must be avoided.
Page 294 Appendix Safety Information Safety Symbols Table 35 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage. Indicates dangerous voltages.
Page 295: The Waste Electrical And Electronic Equipment (Weee) Directive (2002/96/Ec)
Appendix The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Abstract The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC), adopted by EU Commission on 13 February 2003, is introducing producer responsibility on all Electric and Electronic appliances from 13 August 2005.
Page 296: Radio Interference
Appendix Radio Interference Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with equipment unscreened...
Page 297: Sound Emission
Appendix Sound Emission Sound Emission Manufacturer’s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991. This product has a sound pressure emission (at the operator position) < 70 dB. •...
Page 298: Uv-Radiation
Appendix UV-Radiation UV-Radiation This information is only valid for UV-lamps without cover (e.g. 2140-0590 and 2140-0813). N O T E Emissions of ultraviolet radiation (200-315 nm) from this product is limited such that radiant exposure incident upon the unprotected skin or eye of operator or service personnel is limited to the following TLVs (Threshold Limit Values) according to the American Conference of Governmental Industrial Hygienists:...
Page 299: Solvent Information
Appendix Solvent Information Solvent Information Flow Cell To protect optimal functionality of your flow-cell: • Avoid the use of alkaline solutions (pH > 9.5) which can attack quartz and thus impair the optical properties of the flow cell. • If the flow cell is transported while temperatures are below 5 °C, it must be assured that the cell is filled with alcohol.
Page 300 Appendix Solvent Information • Chromatographic grade ethers, which can contain peroxides (for example, THF, dioxane, di-isopropylether) such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides, • Solvents containing strong complexing agents (e.g. EDTA), • Mixtures of carbon tetrachloride with 2-propanol or THF. Agilent 1260 Infinity DAD and MWD User Manual...
Page 301: Declaration Of Conformity For Hox2 Filter
Appendix Declaration of Conformity for HOX2 Filter Declaration of Conformity for HOX2 Filter Agilent 1260 Infinity DAD and MWD User Manual...
Page 302: Installation Of Stainless Steel Cladded Peek Capillaries
Appendix Installation of Stainless Steel Cladded PEEK Capillaries Installation of Stainless Steel Cladded PEEK Capillaries This installation procedure applies for capillaries and corresponding fittings used in N O T E modules delivered before January 2013. For current capillaries and fittings, see “Installing UHP-FF Fittings”...
Page 303: First Step: Finger-Tight Fitting
Appendix Installation of Stainless Steel Cladded PEEK Capillaries First Step: Finger-tight Fitting 1 Tighten the fitting using your fingers. Second Step: Installation to Connector In the second step (“Second Step: Installation to Hard Connectors” page 303 or “Second Step: Installation to Soft Connectors” on page 304), a wrench is used to rotate the fitting relative to the finger-tight position by a defined angle.
Page 304 Appendix Installation of Stainless Steel Cladded PEEK Capillaries First installation of a capillary to a hard connector 1 When tightening a fitting for the first time, start from the finger-tight position (which is not necessarily a vertical wrench position) and rotate the wrench by 135 –...
Page 305 Appendix Installation of Stainless Steel Cladded PEEK Capillaries • to the autosampler needle and • to PEEK columns (like many bio-inert columns). For the installation of bio-inert ZDV unions, see “Installation of the Bio-inert Zero Dead Volume (ZDV) Union” on page 59. First installation of a capillary to a soft connector 1 When tightening a fitting for the first time, start from the finger-tight position (which does not necessarily need to be a vertical wrench position)
Page 306 Appendix Installation of Stainless Steel Cladded PEEK Capillaries Summary for Second Step Table 38 Summary for second step First installation Subsequent installations Step Hard connectors Soft connectors Agilent 1260 Infinity DAD and MWD User Manual...
Page 307: Removing Capillaries
Appendix Installation of Stainless Steel Cladded PEEK Capillaries Removing Capillaries Potential damage of capillaries C AU T I O N ➔ Do not remove fittings from used capillaries. To keep the flow path free of stainless steel, the front end of the capillary is made of PEEK.
Page 308: Agilent Technologies On Internet
Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Agilent 1260 Infinity DAD and MWD User Manual...
Page 309 Index Index beta and alpha line overview remote bio-inert 172, 176, 8-bit configuration switch RS-232 materials on-board LAN BootP service cable installation restart cleaning the module achromat settings compensation sensor open source lens stop compensation sensor short Agilent Lab Advisor software Bootp condensation Agilent Lab Advisor...
Page 310 Index visible lamp voltage run recovery wavelength calibration failed information wavelength recalibration lost drift (ASTM) and noise 24, 27, 30, on uv-radiation DSP not running initialization mode selection installation fan failed bench space features electrical connections delivery checklist safety and maintenance 35, 26, descriptions of environment...
Page 311 Index what you have to do first no run data available in device damaged remote timeout leak sensor open parts identification uv heater current cables leak sensor short uv ignition failed for maintenance leak uv lamp current peak width (response time) correcting uv lamp voltage performance...
Page 312 Index RS-232C noise and drift (ASTM) 33, 24, 27, time constant versus response time cable timeout noise and linearity run recovery troubleshooting performance 33, 24, 27, automatic error messages 125, programmable slit width 33, 24, manual status indicators 119, no run data available in safety and maintenance 35, 26, unpacking...
Page 314 • introcduction and specifications, • installation, • using and optimizing, • troubleshooting and diagnose, • maintenance, • parts identification, • safety and related information. © Agilent Technologies 2006-2017, 2018 Printed in Germany 07/2018 *G1315-90015* *G1315-90015* G1315-90015 Rev. C Agilent Technologies...

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1260 infinity g1365d 1260 infinity g1315d 1260 infinity g1365c

Agilent Technologies 1260 Infinity G1315C User Manual

Table of Contents

2 Site Requirements and Specifications

3 Installing the Module

4 Using the Detector

5 How to Optimize the Detector

6 Troubleshooting and Diagnostics

7 Error Information

8 Test Functions

9 Maintenance

10 Parts for Maintenance

12 Hardware Information

13 LAN Configuration

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