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Zeiss LSM 510 Brief Operating Manual
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Zeiss LSM 510 Brief Operating Manual

Zeiss LSM 510 Brief Operating Manual

Laser scanning microscopes
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Download this manual See also: Operating Manual, User Manual
LSM 510 and LSM 510 META
Laser Scanning Microscopes
Brief Operating Manual
Release 3.5
July 2005

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Summary of Contents for Zeiss LSM 510

  • Page 1 LSM 510 and LSM 510 META Laser Scanning Microscopes Brief Operating Manual Release 3.5 July 2005...

  • Page 2: Table Of Contents

    Observe the following instructions: − The LSM 510 and LSM 510 META laser scanning microscope, including its original accessories and compatible accessories from other manufacturers, may only be used for the purposes and microscopy techniques described in this manual (intended use).

  • Page 3: Starting The System

    Starting the System Switching on the LSM system • When set to ON the REMOTE CONTROL switch labeled System/PC provides power to the microscope and the computer. This allows to use the microscope and the computer without running the LSM Software (Fig. 1). •...
  • Page 4 Starting the LSM 5 software program • Double click the LSM 510 icon on the desktop of WINDOWS to start the LSM 5 software program. LSM 510 The LSM 510 Switchboard window appears on the screen (Fig. 3). Fig. 3 LSM 510 Switchboard menu •...
  • Page 5 Creating a database for acquired images • Click on the File button in the Main menu toolbar. The File subordinate toolbar appears in the Main menu. Fig. 5 Main menu with File subordinate toolbar • Click on the New button in the File subordinate toolbar. The Create New Database window appears.

  • Page 6: Setting The Microscope

    • Click on the LSM button to set the microscope screen observation via laser excitation using the LSM 510 and software evaluation. Setting the microscope and storing the settings • Click on the VIS button for direct observation.
  • Page 7 Selecting an objective • Open the graphical pop-up menu by clicking on the Objective button (Fig. 10). • Click on the objective you want to select. The selected objective will automatically move into the beam path. Focussing the microscope for transmitted light •...

  • Page 8: Configuring The Beam Path And Lasers

    Configuring the beam path and lasers • Click on the LSM button in the Acquire subordinate toolbar for laser scanning. Choosing the configuration Single Track − Use for single, double and triple labeling; simultaneous scanning only Advantage: faster image acquisition −...
  • Page 9 • Select the appropriate filters and activate the channels. • Click the Excitation button to select the laser lines attenuation values (transmission in %) in the displayed window. For the configuration of the beam path, please refer to the application-specific configurations depending on the used dyes and markers and the existing instrument configuration.
  • Page 10 • Click on the Multi Track button in the Configuration Control window (Fig. 15). • Click on the Descanned button if necessary. The following functions are available in the List of Tracks panel (Fig. 15). Modes Switch tracks after each Line button Tracks are switched during scanning line by line.

  • Page 11: Scanning An Image

    Scanning an image • Click on the Scan button in the Acquire subordinate toolbar to open the Scan Control window. The Scan Control window appears (Fig. 16). Setting the parameters for scanning • Select Mode in the Scan Control window. •...
  • Page 12 In the Sum method, the pixel values of all scans are only added up, without a mean value being calculated. • Select the Number for averaging. Continuous averaging is possible in the Frame mode. In this case a Finish button for ending continuous averaging is displayed instead of the Cont.
  • Page 13 Image optimization Choosing a lookup table • Select Palette in the Image window of the scanned image (Fig. 19). The Color Palette window appears. • In the Color Palette List panel, click on the Range Indicator item (Fig. 20). The scanned image appears in a false-color presentation (Fig.
  • Page 14 Scanning a Z stack • Select Z Stack in the Scan Control window. • Select Frame if necessary. The Z Settings panel appears. • Select Mark First/Last on the Z Settings panel. • Click on the XY cont button. A continuous XY-scan of the set focus position will be performed.

  • Page 15: Storing An Image

    Storing an image • Click on the Save or Save As button in the Image window or in the File subordinate toolbar of the Main menu. The Save Image and Parameter As window appears. Fig. 23 Save Image and Parameter As window •...

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Lsm 510 meta