Running Samples; Running A Sample; Injection Volume - Agilent Technologies agilent 7683B Installation, Operation And Maintenance Manual

7

Running Samples

Running a Sample

When running a sample, keep your hands away from the syringe needle. The needle
WA R N I N G
is sharp and may contain hazardous chemicals.
To operate your automatic liquid sampler:
1 Install a clean syringe. See
2 Fill the solvent bottles. See
3 Place the solvent and waste bottles in the turret. See
4 Load the sample vials into the turret or the tray. See
5 Prepare the GC sequence. See your GC or ChemStation documents.
6 Run the sequence by pressing the start button on the GC. On a 5890 GC you

Injection volume

The injection volume depends on the sample size and the syringe size.
• Syringe size may be 1 µL, 5 µL, 10 µL, 25 µL, 50 µL, or 100 µL.
• Sample size may be 2%, 10%, 20%, 30%, 40%, or 50% of the syringe size.
After syringe size is selected, sample sizes are shown in microliters
Table 21
Syringe size, µL
* Nanoliter adapt must be set to on for 6890 GC.
130
page 116.
Bottles in the Sampler"
Vials" on page 110.
may optionally press the start button on the ALS controller. When the GC is
ready, the ALS begins the injections.
Syringe and sample sizes
Available sample sizes, µL
*
2%
1 0.02
5 0.1
10 0.2
25 0.5
50 1.0
100 2.0
"Installing a Syringe"
"Preparing Solvent and Waste Bottles"
on page 118.
10% 20% 30%
0.1 0.2 0.3
0.5 1.0 1.5
1.0 2.0 3.0
2.5 5.0 7.5
5.0 10 15
10 20 30
Installation, Operation, and Maintenance
on page 103.
on
"Placing Vials and
"Preparing Sample
(Table
40% 50%
0.4 0.5
2.0 2.5
4.0 5.0
10.0 12.5
20 25
40 50
Part 2, Operation
21).