Nikon A1 Instruction Manual page 87

Confocal microscope
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Chapter 27. Capturing Simultaneous Photo Activation Imaging (Resonant & Galvano Scanner)
27.3
Set the laser light for activation.
(1) Click [Photo Activation] to switch the setting window.
(2) Click Tab 1 (Stimulation Group 1 setting).
(3) Select lasers used for activation.
Note: During simultaneous photo activation observation, 405 nm and 488 nm lasers can be used. To
use 488 nm lasers, however, the filter for 488 nm simultaneous activation needs to be installed.
(4) Move the laser bar to select the laser power for activation.
(5) Select [Scan Speed] for activation.
Note: Consider that Scan Speed is the time required for a single activation.
When "1 Sec/Frame" is selected, the time for a single activation is one second.
(3)
(6) When there are Stimulation Groups 2 and 3, repeat Steps (2) to (5).
27.4
Set time series for photo activation simultaneous imaging.
(1) Click the
to open the ND Stimulation dialog box.
(2)
(1)
(5)
[Photo Activation] button
87
(4)
A1

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