Sign In
Upload
Manuals
Brands
SCIEX Manuals
Control Unit
CESI 8000 Plus
SCIEX CESI 8000 Plus Manuals
Manuals and User Guides for SCIEX CESI 8000 Plus. We have
4
SCIEX CESI 8000 Plus manuals available for free PDF download: User Manual, Preinstallation Manual, Site Planning Manual
SCIEX CESI 8000 Plus User Manual (376 pages)
Brand:
SCIEX
| Category:
Laboratory Equipment
| Size: 17 MB
Table of Contents
Table of Contents
3
CHAPTER 1 Safety, Notices and Labels
13
Alerts for Warning, Caution, Important, and Note
14
Instrument Safety Precautions
15
Moving Parts or Sharp Objects
16
Electrical Safety
16
Laser Safety (for Optional Laser Device)
17
Class 1 Laser Caution Label
17
Chemical Precautions
17
Safety Symbols and Labels
18
High Voltage Electric Shock Risk Symbol
18
Attention Safety Symbol
18
Sharp Object Label
18
Mobile Cart Caution Label
19
Cancer and Reproductive Harm Label
19
Rohs Notices
19
China Rohs Caution Label
19
Other Instrument Labels
20
Recycling Label (WEEE)
20
Disposal of Devices Containing Mercury Components
21
CE Mark Label
21
RCM Mark Label
21
CSA Mark Label
21
Overview
13
安全、通知和标签
23
综述
23
警告、注意、重要事项以及注释的提示
24
仪器安全防护措施
25
活动部件或尖锐物
26
电气安全
26
激光安全 (适用于可选激光设备
26
类激光小心标签 (1级激光警告标签)
26
化学品注意事项
27
安全符号和标签
27
高压电击危险符号
27
注意安全符号
27
尖锐物标签
28
流动车警告标签
28
致癌和生殖危害标签
28
Rohs 通知
28
中国 Rohs 警告标签
29
其他仪器标签
29
环保标签 (Weee)
29
处理包含加汞组件的设备
30
Ce 标志标签
30
Rcm 标志标签
30
Csa 标识标签
30
CHAPITRE 3 Sécurité, Consignes Et Étiquettes
31
Alertes « Avertissement, Mise en Garde, Important Et Remarque
32
Précautions de Sécurité Pour L'instrument
33
Pièces Mobiles Ou Objets Tranchants
34
Sécurité Électrique
34
Sécurité Laser (Pour Les Appareils Avec Laser en Option)
35
Étiquette de Mise en Garde : Produit Laser de Classe 1
35
Précautions Chimiques
36
Symboles Et Étiquettes de Sécurité
36
Symbole « Haute Tension, Risque de Choc Électrique
36
Symbole de Sécurité Attention
37
Étiquette Objet Tranchant
37
Étiquette de Mise en Garde Chariot Mobile
37
Étiquette Concernant Les Risques de Cancer Et de Malformations Congénitales
37
Autres Étiquettes de L'instrument
38
Étiquette de Recyclage (WEEE)
39
Élimination des Appareils Contenant des Composants Au Mercure
39
Étiquette Marque CE
39
Étiquette Marque MRC
40
Étiquette Marque CSA
40
Consignes Rohs
38
Étiquette de Mise en Garde Rohs (Chine)
38
About this Manual
41
Optional Detectors
41
Présentation
31
CHAPTER 4: System Overview
41
System Overview
41
Introduction
42
Instrument
43
Sample Handling System
44
Syringe Pump, Power Supply, Leds and Interlock
46
Syringe Pump
46
High Voltage (HV) Power Supply
46
LED Indicators
46
Cartridge and Sample Cover Interlocks
47
Optims Cartridge
48
CHAPTER 5 Integrating the CESI 8000 Plus System with a Mass Spectrometer
51
CESI 8000 Plus System: Installing Optims Cartridge
53
Optims Cartridge Installation
53
Thermo Scientific Mass Spectrometer: Integration with the CESI 8000 Plus System
57
Physical Integration with the CESI 8000 Plus System
57
Optims Cartridge Sprayer Tip Installation into a Thermo Scientific Nanospray II Ion Source
59
Scientific Nanospray II Ion
60
Establishing Communication between the CESI 8000 Plus Controller and the Thermo Scientific Mass Spectrometer
65
Aligning the CESI 8000 Plus System with a Thermo Scientific Mass Spectrometer
66
SCIEX Tripletof ® 5600 Mass Spectrometer: Integration with the CESI 8000
71
Plus System
71
Physical Connections with the CESI 8000 Plus System
71
Optims Cartridge Sprayer Tip Installation into the SCIEX Nanospray
73
Source
73
Establishing Communication between the CESI 8000 Plus Controller and the SCIEX Mass Spectrometer
80
Aligning the CESI 8000 Plus System with the SCIEX Tripletof
81
5600 Mass Spectrometer
81
CHAPTER 6 32 Karat Software Overview
87
Overview of 32 Karat Software
87
System Administration
87
Controller and Instrument Start up
88
Controller/Network Login
88
License Key
89
Demo Mode
89
Launching 32 Karat Software
89
Instrument Start up
89
Instruments and Projects
90
Configuration
90
Online Versus Offline
90
Using the Direct Control Window to Load the Cartridge and Samples
91
Tripletof ® 5600 Mass Spectrometer Using AAO
93
Methods and Sequences
94
Creating and Editing a Method
95
Instrument Setup Window
95
Instrument Setup - Initial Conditions
95
Instrument Setup - Time Program
97
Separate Dialog
98
Rinse Dialog
100
Inject Dialog
100
Stop Data
102
End
102
Saving a Method
102
Overview of Creating a Sequence
103
Single Run and Sequence Runs
103
Programming a Sequence
104
Sequence Table
108
Sequence Validation
109
CHAPTER 7 CESI 8000 Software
111
Overview of the CESI 8000 Software
111
Launching the CESI 8000 Software
112
Login and Logout
113
Lock and Unlock
114
About Dialog
114
Help
114
Users
114
Application Selection
115
Run Application
115
Samples and Vials
118
Samples/Vials Dialog
118
Insert Option
120
Sequence Dialog
121
Sequence Table Rows
123
Fill down Option
127
Display Options
128
Vial Legend
129
Tray Detail View
129
Vial Preview
130
Run Acquisition
131
SCIEX Tripletof ® 5600 Mass Spectrometer Using AAO
133
Graph Options
134
Sequence Transfer
135
Describe Sequence
141
Sequence Verification
144
Sequence Transfer Overview
135
Sequence Display Options
144
CHAPTER 8 Using a SCIEX Tripletof 5600 Mass Spectrometer with the CESI 8000 Plus System
147
Stock Reagents and Other Consumables Required
148
Preparation of Reagent Solutions and Test Sample
149
Preparation of 10% (V/V) Hac-Background Electrolyte (BGE)
150
Preparation of 20% (V/V) Acetic Acid (Hac)-For LE Ph Adjustment
150
Preparation of 200 MM LE (Leading Electrolyte) Buffer
150
Reconstitution of 1 ΜM Beta-Galactosidase Digest Solution (Beta-Galactosidase Stock Solution)
151
Preparation of Beta-Galactosidase Test Sample
151
Preparation of Beta-Galactosidase for Auto-Calibration
152
Buffer Tray Setup
152
Sample Tray Setup
153
Procedure for Installation of Adapter and Optims Cartridge
154
Methods for the CESI 8000 Plus System
155
CESI-MS Beta-Galactosidase System Performance Test
159
Capillary Conditioning
162
Establishing a Stable Spray and Determining Optimum ESI Voltage
165
Running CESI-MS
172
Create a Sequence in the Analyst
173
TF Software
173
Start a Sequence in the Analyst TF Software
176
Load Sample and Reagents on the CESI 8000 Plus System
177
Sample Tray Setup
177
Create a Sequence in the CESI 8000 Software
179
Start a Sequence in the CESI 8000 Software
183
Automatic Rinse Feature
184
Beta-Galactosidase Analysis
185
Introduction
189
Using a Thermo Scientific Mass Spectrometer with the CESI 8000 Plus System
189
CESI-MS Integration Procedures
51
Introduction
147
CHAPTER 9: Using a Thermo Scientific Mass Spectrometer with the CESI 8000 Plus System
190
Preparation of 10% (V/V) Acetic Acid (Hac)-Background Electrolyte (BGE)
191
Required
190
Sample
191
Preparation of 20% (V/V) Acetic Acid (Hac) - for LE Buffer Ph Adjustment
192
Buffer
192
Reconstitution of 1 ΜM Beta-Galactosidase Digest Solution
193
Solution)
193
Sample
193
Test
193
Cartridge
194
Capillary Conditioning
195
Buffer Tray Setup
195
Procedure
196
Voltage
198
Tuning
202
Running CESI-MS
203
Software
204
Software
207
Software
208
System
209
Software
211
Create a Sequence in the CESI
211
Automatic Rinse Feature
212
Software
213
Start a Sequence in the CESI
213
Beta-Galactosidase Analysis
214
CHAPTER 10 Instruments
217
Shutdown
217
Shutdown and Decoupling Overview
217
Decoupling the CESI 8000 Plus System
222
Spectrometer
222
Decoupling from the SCIEX Mass
225
Spectrometer
225
APPENDIX A: Specifications
229
CESI 8000 Plus System
229
Cart Specifications
230
Validated Controller Configuration
230
Capillary Temperature Control
231
Pressure and Vacuum System
231
Sample Temperature Control
231
Detector Specifications
232
UV Detector
232
Laser Induced Fluorescence (LIF) Detector (Optional)
233
Photo Diode Array (PDA) Detector (Optional)
233
APPENDIX B: Nominal Network Configuration
235
Overview
235
Systems
236
Switching from a SCIEX Mass Spectrometer to Another Mass
237
Spectrometer
237
Switching to a SCIEX Mass Spectrometer from Another Mass
239
Spectrometer
239
Advanced Networking and Computer Configuration for the Analyst ® TF
241
Software
241
CE-MS Networking
241
PC Configurations
241
Network Sharing Setting for the Crossover Connection
262
Setting the Mass Spectrometer Type
264
Curtain Gas Patch Installation
265
CE-MS Driver Installation
267
Saving Network Configurations
269
Analyst Hardware Profile Configuration
270
To Configure the Mass Spectrometer Hardware Profile
270
Curtain Gas Setting and Verification
276
Curtain Gas Setting
276
Curtain Gas Verification
278
APPENDIX C: Tripletof5600 Mass Spectrometer
281
Overview
281
Required Reagents
281
Reagent and Sample Preparation
281
Manual Calibration before Auto-Calibration
282
Create a Reference Table for CE-MS Calibration
282
Manual Calibration of the Mass Spectrometer
285
Manual Calibration in TOF Mass Spectrometer Mode
286
Manual Calibration in Product Ion Mode
292
Tripletof ® 5600 Mass Spectrometer Manual and Auto-Calibration Methods
281
Methods for Auto-Calibration
296
CE Method for Auto-Calibration
296
MS Method for Auto-Calibration
297
Executing Auto-Calibration
300
Create a Sequence in the Analyst TF Software
300
Start a Sequence in the Analyst
303
TF Software
303
System
305
Setup
305
Sample Tray Setup
305
Software
306
Create a Sequence in the CESI
306
Software
310
Start a Sequence in the CESI
310
Auto-Calibration Data Analysis
312
Feature
312
Automatic Rinse Feature
312
Failure
317
Auto-Calibration Troubleshooting
318
Table
319
APPENDIX D: Auto Vial Increment Feature
321
Feature
321
Overview
321
Step
321
Injection
322
Step
324
Method
326
Sequence
327
Conditioning
329
Conductive Liquid Capillary
329
Conductive Liquid Capillary (CLC) Conditioning
329
Procedure
329
APPENDIX E: Conductive Liquid Capillary Conditioning , 329
331
APPENDIX F: Using Vials and Hardware Maintenance
331
Maintenance
331
Overview
331
Using CESI-MS Vials and Caps
332
Vials
332
Vials
333
Capillary Cleaning Procedure
334
Procedures
334
Storage)
334
Procedure
335
Plate
336
Block
338
Block
341
Refilling Coolant
343
Rings
344
Replacing Fuses
346
APPENDIX G: Troubleshooting
349
Diagrams
349
Sample Preparation Considerations for Analysis with CESI-MS
350
Properties of a Method or Sequence Cannot be Modified or Saved
350
Tip
351
Possible Cause
351
Possible Solution
351
Blocked Sprayer Tip
351
Electrospray Cannot be Established between the CESI 8000 Plus System and Mass Spectrometer
353
Electrospray Is Detected at Zero Ion Spray (IS) Voltage While Applying CE Voltage
354
Unstable Electrospray While Applying CE Voltage and IS Voltage
355
Droplet at Capillary Tip Disappeared Indicating that Electrospray Has Started, but no Mass Spectrometer Signal Is Detected
356
Signal of Total Ion Current (TIC) Is Lower than Usual And/Or Background Signal Intensity Fluctuates more than 40
357
Unstable CE Current
358
No Analyte Signals Detected by Mass Spectrometer During a CE-MS Separation
359
Some Analyte Peaks Are Missing or Smaller than Expected
360
CE Current Gradually Decreases During CE-MS Separation
361
The Iron-Acetate Cluster M/Z 537 Is Suppressing the Sample Signals
362
High Voltage System Unable to Deliver the Required Current
363
Overview
349
Procedure
349
Troubleshooting
349
Fine Tuning Sprayer Tip Position for Thermo Scientific Mass Spectrometer
364
Fine Tuning Sprayer Tip Position for SCIEX Mass Spectrometer
366
CESI 8000 Plus Mobile Cart Homing Procedure
368
Contact Us
369
Customer Training
369
Cybersecurity
369
Online Learning Center
369
SCIEX Support
369
Documentation
370
Index
371
Advertisement
SCIEX CESI 8000 Plus User Manual (247 pages)
High Performance Separation-ESI Module
Brand:
SCIEX
| Category:
Control Unit
| Size: 21 MB
Table of Contents
Table of Contents
3
Operational Precautions and Limitations
8
General Safety Information
8
Documentation Symbols and Conventions
8
Regulatory Compliance
9
Australia and New Zealand
9
Canada
9
Europe
9
United States
10
International
10
Electrical Precautions
10
Mains Supply
10
Protective Earth Conductor
11
Chemical Precautions
11
System Safe Fluids
12
Physical Precautions
12
Environmental Precautions
12
Electromagnetic Environment
13
Decommissioning and Disposal
14
UV Radiation Precautions
14
Laser Precautions
14
Qualified Personnel
15
Laboratory Conditions
15
Safe Environmental Conditions
15
Performance Specifications
16
Equipment Use and Modification
16
Introduction
17
Description
17
Hardware Overview
19
Sample-Handling System
20
Syringe Pump
22
High-Voltage Power Supply
22
LED Indicators
23
Cartridge and Sample Cover Interlocks
23
Optims Cartridge
25
Mass Spectrometer Adapters
28
Required Reagents and Materials
28
Customer-Supplied Equipment and Supplies
29
Set up the Sytem
30
Install the Cartridge
30
Connect the GPIB-USB Cable
35
Connect an Auxiliary I/O Cable
37
Change the Mass Spectrometer Type
37
Configure the Hardware Profile
38
Install the Adapter
46
Connect the High-Voltage Cables
51
Align the System with the Mass Spectrometer
52
Prepare the Reagents
60
Prepare the 10% Hac Solution
60
Prepare the 20% Hac Solution
60
Prepare the 200 MM le Buffer, Ph 4.0
61
Prepare the Pepcalmix Test Sample
61
Prepare the Pepcalmix Solution for Direct Infusion
61
Prepare the Peptide Test Sample
62
Prepare the Trays
63
About Vials
63
Fill a CESI Vial
64
Fill a Microvial
65
Fill a Nanovial
66
Prepare the Buffer Trays
67
Prepare the Sample Tray
68
Prepare the System
70
Condition the Capillaries
70
Establish a Stable Spray
72
Optimize the ESI Voltage
75
Fine-Tune the Position of the Sprayer Tip
79
Manual Calibration
80
Prepare for Manual Calibration
80
Create a Reference Table
81
Manually Calibrate in TOF MS Mode
85
Manually Calibrate in Product Ion Mode
89
Run Workflow
94
Methods for the Silica Surface Optims Cartridge
94
Initial Conditions Tab
95
Time Program Tab
95
Do a System Suitability Test
97
Create a Sequence in the 32 Karat Software
102
Create a Batch in the Analyst TF Software
106
Start the Batch in the Analyst TF Software
111
Load the Trays
112
Start the Sequence in the 32 Karat Software
113
Analyze the Data from the Pepcalmix Test Sample
115
About Autocalibration
117
Create the MS Acquisition Method for Autocalibration
118
Add Autocalibration to the Sequence
123
Analyze the Autocalibration Data
125
Shutdown and Disconnection
131
Remove the Cartridge
131
Store the Cartridge
135
Disconnect the CESI 8000 Plus System from the Mass Spectrometer
135
Maintenance
138
Remove and Install the Interface Plate
138
Interface Block, Electrodes, and Insertion Levers
141
Replace the Insertion Levers
141
Replace the Electrodes
142
Clean the Electrodes, Insertion Levers, and Interface Block
144
Add Capillary Cartridge Coolant
145
Replace the Quad Rings
147
Replace the Fuses
148
Set the Home Position for the Mobile Cart
150
Troubleshooting
151
Clean Blockage from the Sprayer Tip
151
Conductive Liquid Capillary Contamination
151
Condition the Conductive Liquid Capillary
152
Autocalibration Troubleshooting
153
No Electrospray between CESI 8000 Plus System and Mass Spectrometer
155
Electrospray Is Detected When ESI Voltage Is Zero
156
Electrospray Is Unstable When Voltage Is Applied
157
Droplet at Capillary Tip Disappeared but no Mass Spectrometer Signal Is Detected
158
TIC Signal Is Lower than Usual And/Or Intensity of Background Signal Fluctuates too Much
159
Electrical Current Is Unstable
160
Analyte Signals Are Not Detected by Mass Spectrometer During Separation
161
Some Analyte Peaks Are Missing or Smaller than Expected
162
Current Gradually Decreases During Separation
163
Iron-Acetate Cluster M/Z 537 Is Suppressing Sample Signals
164
High-Voltage System Cannot Deliver Required Electrical Current
165
Order Parts
166
Cartridges and Parts
166
A 32 Karat Software
170
System Administration
170
Controller and System Start-Up
170
Controller and Network Login
170
License Key
171
Start the CESI 8000 Plus System
171
Open the 32 Karat Software
171
Systems and Projects
173
Online and Offline Modes
173
Open the Direct Control Window
174
Create a Toolbar Icon for the Direct Control Window
175
32 Karat Software Methods
175
Create or Change a Method
176
Single Run and Sequence Runs
185
32 Karat Software Sequences
186
Sequence Table Columns
186
Sequence Validation
188
Sequence Transfer
188
About the Auto-Increment Feature
193
Set Auto-Increment for a Rinse Event
193
Set Auto-Increment for an Inject Event
195
Set Auto-Increment for a Separate Event
196
Auto-Increment in a Method
198
Review Vial Positions before a Sequence Is Run
199
B CESI 8000 Plus Software
201
User Roles and Privileges
201
Open the CESI 8000 Plus Software
201
Run an Application
204
Samples/Vials Window
206
Set up Samples and Vials for a Method
206
Set up Samples and Vials for a Sequence
209
Display Options Menu
219
Acquisition Window
223
During a Run
224
Stop a Run
225
Complete a Run
226
Graph Options
226
Describe Sequence Window
227
Describe the Sequence
229
C Connect an Ethernet Cable
231
D System Specifications
233
CESI 8000 Plus System Specifications
233
Supplied Controller Configuration
234
Mobile Cart Specifications
234
Capillary Temperature Control Specifications
235
Sample Temperature Control Specifications
235
Pressure and Vacuum System Specifications
235
Detector Specifications
236
UV Detector Specifications
236
(Optional) LIF Detector Specifications
237
(Optional) PDA Detector Specifications
238
E Glossary of Symbols
239
F Glossary of Warnings
245
Contact Us
246
Customer Training
246
Online Learning Center
246
Purchase Supplies and Reagents
246
SCIEX Support
246
Cybersecurity
246
Documentation
246
SCIEX CESI 8000 Plus Preinstallation Manual (24 pages)
High Performance Separation-ESI Module
Brand:
SCIEX
| Category:
Control Unit
| Size: 0 MB
Table of Contents
Table of Contents
3
Chapter 1 Introduction
5
Customer Site Planner Responsibilities
5
FSE Responsibilities
6
During Installation
6
Operator Familiarization
6
Technical Support
6
Chapter 2 Site Planning Checklist
7
Customer Information
7
Requirements
7
Site Layout
7
Electrical Requirements
8
Ventilation and Waste Collection Requirements
8
Environmental Requirements
8
Biosafety Requirements
9
Customer Preparation
9
Mass Spectrometer Requirements
9
Solutions and Equipment Requirements
9
Mass Spectrometer Information
10
Customer Profile
10
Comments and Exceptions
11
Signoff
11
Appendix A Site Requirements
12
Site Layout Requirements
12
Laboratory Layout and Clearances
12
Height Requirements
13
Weights and Dimensions
13
Electrical Requirements
14
Mains Supply Connections
14
Mains Supply Fluctuations
14
Protective Earth Conductor
14
Surge Suppression
14
System Electrical Specifications
14
Ventilation and Waste Collection Requirements
15
Ventilation Requirements
15
Waste Collection Requirements
15
Environmental Requirements
15
Biosafety Requirements
16
Customer Preparation Requirements
16
Mass Spectrometer Requirements
16
SCIEX Mass Spectrometers
16
Thermo Scientific Mass Spectrometers
17
Bruker Mass Spectrometers
17
Waters Mass Spectrometers
17
Customer-Supplied Solutions and Equipment
17
Appendix B Equipment Safety Categories
19
Appendix C System Specifications
20
Instrument Specifications
20
Cart Specifications
20
Controller Specifications
21
Detector Specifications
21
(Optional) UV Detector Specifications
21
(Optional) Laser Induced Fluorescence (LIF) Detector Specifications
22
(Optional) Photo Diode Array (PDA) Detector Specifications
22
Revision History
24
Advertisement
SCIEX CESI 8000 Plus Site Planning Manual (21 pages)
High Performance Separation-ESI Module
Brand:
SCIEX
| Category:
Laboratory Equipment
| Size: 0 MB
Table of Contents
Table of Contents
3
1 Introduction
5
Customer Site Planner Responsibilities
5
FSE Responsibilities
6
During Installation
6
Customer Familiarization
6
Technical Support
6
2 Site Planning Checklist
7
Customer Information
7
Requirements
7
Site Layout
7
Electrical Requirements
8
Ventilation and Waste Collection Requirements
8
Environmental Requirements
9
Computer, Network, and Software Requirements
9
Mass Spectrometer Requirements (CESI-MS Systems Only)
9
Solutions and Equipment Requirements
10
Customer Preparation
10
Mass Spectrometer Information
11
Customer Profile
11
Comments and Exceptions
12
Signoff
12
A Site Requirements
13
Site Layout Requirements
13
Laboratory Layout and Clearances
13
Weights and Dimensions
14
Electrical Requirements
15
Mains Supply Connections
15
Mains Supply Fluctuations
15
Protective Earth Conductor
15
System Electrical Specifications
15
Ventilation and Waste Collection Requirements
16
Environmental Requirements
16
Biosafety Requirements
17
Computer, Software, and Network Requirements
17
Controller Requirements
17
Printer Requirements
17
LAN Connection (Optional)
17
Customer-Supplied Solutions and Equipment
17
Customer Preparation Requirements
18
Mass Spectrometer Requirements (CESI-MS Systems Only)
18
SCIEX Mass Spectrometers
19
Thermo Scientific Mass Spectrometers
19
Bruker Mass Spectrometers
20
Waters Mass Spectrometers
20
B Equipment Safety Categories
21
Advertisement
Related Products
SCIEX C100HT
SCIEX Categories
Laboratory Equipment
Portable Generator
Medical Equipment
Water Pump
Control Unit
More SCIEX Manuals
Login
Sign In
OR
Sign in with Facebook
Sign in with Google
Upload manual
Upload from disk
Upload from URL