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MXG102
GeminiBio MXG102 Manuals
Manuals and User Guides for GeminiBio MXG102. We have
1
GeminiBio MXG102 manual available for free PDF download: User Manual
GeminiBio MXG102 User Manual (93 pages)
Brand:
GeminiBio
| Category:
Laboratory Equipment
| Size: 18 MB
Table of Contents
Table of Contents
3
Introduction
6
About the User Guide
6
Conventions Used in the User Guide
7
Safety Precautions
7
Electrical Safety
7
Laser Safety
7
Biological Safety
8
Moxi Go II™ System Overview
9
Getting Started
11
General Guidelines
11
Additional Materials Required
12
Using the Moxi Go II Tm
13
Home (Start) Screen
13
Settings
15
Date and Time
15
Passcode Lock
15
Fluorescence Gain
16
Auto Noise-Find
16
Auto Gate Settings
16
Sample Preparation Considerations
16
Running a Test
18
General Instructions (All Tests Except "Cell QC", "Open Flow" and "Multiplex Bead Assay")
18
Inserting a Cassette
19
System Calibration
19
Loading a Sample
20
"Open Flow Cytometry" Test
21
GO Flow" Tests (Simplified Version of "Open Flow")
23
"Cell Qc" Test
24
Cell QC - Single Run
24
Cell QC - Start Batch
25
Cell QC - Initial Test Output (both Single Run and Batch)
26
Cell QC - Test Output - Continuing Batch Testing
27
Cell QC - Batch Run - Saved Data and .CSV Summary File
28
Cell QC - "Add to Existing Batch
29
Multiplex Bead Assay
29
Overview - Current State
29
Running a Multiplex Reference Sample
30
Running a Multiplex Unknown Sample
32
Locating All Unknown Tests Linked to a "Reference" Sample
33
Test Results
35
Recorded Channels
35
Data Display Formats - General Output Types
35
Fluorescence Vs Size Scatter Plot
36
Histograms
37
Fluorescence Vs. Fluorescence Scatter Plot
38
Reading Raw Data Output (Conc., Size, MFI, and %)
39
Fluorescence Vs. Size Scatter (Dot) Plot
39
Single Channel Histogram Plot
39
Fluorescence Vs. Fluorescence Scatter (Dot) Plot
40
Simplified Output and Auto-Gating Overview
40
"Pbmc Check" Output
41
"CAR-T Expansion" Output
42
Cell QC" - Output (both Single Run and Batch)
44
Quadrant - Data Summary Table Output (Apoptosis, GFP, Cell Health)
45
Analyzing/Gating the Data
45
(General) Recommended Post-Test Workflow
46
Re-Scaling the X Axis (Size Range)
47
Gating the Data
47
Overview
47
Size Gating of Scatter (Dot) Plots
49
Fluorescent Gating of Scatter (Dot) Plots
49
Noise Gating of Scatter (Dot) Plots
50
"Web" Or "Quadrant" Gating - Fluorescence Vs. Fluorescence Scatter Plots
51
Store and Recall of Gate Settings
52
Histogram Overlays on Fluorescence Vs Size Scatter Plots
53
"Size Histogram" Assay
53
Changing Display Axis for Tests (PMT Vs Size, Histogram Only Views, "Web" or Quadrant Views)
55
File/Test Operations
57
Test Naming and Renaming Tests
57
Saving and Deleting Data/Tests
57
Exporting Data Screenshots
58
Editing Saved Tests
58
Compare/Overlay Saved Tests
60
Compensation (of Spillover)
62
Overview/Background
62
Moxi GO II TM Compensation
62
General Workflow
62
Standard - Single Test - Compensation
63
Compensation with Negative Control (Load Control Set)
66
Compensation - Future Tests
67
Exporting Data - Connecting Via USB (USB On-The-Go)
69
Connect the Moxi GO II TM to a Computer
69
Put the Moxi GO II TM in USB Connect Mode
70
Safely Disconnect Moxi Go II
71
TM from the Computer
71
Moxi Go II Tm Apps
72
Instrument Firmware and Software Upgrades
74
Moxi Go II - Tray Insert Replacement Procedure
76
Background
76
System Requirements and Ordering Information
76
Removing an Existing Insert
77
Installing an Insert
79
Troubleshooting
81
Maintenance and Storage
85
Specifications for the Moxi GO II
88
And Cassettes
88
Ordering Information
90
Technical and Customer Service
90
Warranty
91
Applicable Patents
92
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